Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0011260

Physical Location & Seq

Genomic Coordinates Reverse (-)

360241 .. 364651

Gene Structure 4411 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG005700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGTTATGGTGCGGTCGCCATGTTATTGACCAATTCAGAACTCTACGCGCACAGGCCTTTTCATCAAACCCTCTTCACTACTACTGTAACTACACAAATCTCCTTGAAGCCTGCATTCGATCTAAATCTCTCGCTCAAGCCAAGATAATCCATCAACTTGTTATAAAAGACAATACCCAGATCGAGGACCGGTCTCTTCTACTTGAAAAACTAGCCCGGCTCTACTTGACCTGTAATCAAGTTGAGGTCGCACGCCGAGTGTTCGACGAAATTCCCCAACCAAATGTTATTATTTGGAACCTATTGATAAGAGCCTATGCATGGAACGGACCGTTTGAACGAGCCATTGAGTTGTACTATGGCATGCTCGAACTGGGCGTTAAACCAAGCAAGTACACGTACCCTTTTGTTCTCAAGGCATGCTCGGGTTTGCAGGCACTACACGTTGGTCAAGACATTCATCAACATGCGAAGCGACTTGGGCTCGGCTCTGACGTCTATGTTTGCACTGCTTTGATTGATTTGTACGCAAAATGTGGAGACTTGGTAGCTGCGCAGAGTGTGTTTGATGGTATGCTGTATAAAGATGTTGTGGCGTGGAATGCTATGATTGCTGGGTTTTCGCTTCATGGTCTTTGTGGTGACACTGCAGAGTTGCTGGTTCAAATGCAGAGAGCGGGAACTGCCCCGAATGCCTCTACCATTGTTGCGGTTCTTCCCACAGTTGCGCAAGCTAACGCGCTGAGCCAAGGGAAGGCCATACATGGTTACTCCATGAGGAGGAGGTTTAGTGATGAGGACGTGGTACTTAGTACTGCGCTTTTGGATATGTATGCTAAATGTCAGTGCATAGTTTACGCGAGGAGAATCTTTGATGCAATGAGTGTCAGGAATGAGGTTTGTTGGAGTGCGATGATAGGCGCATATGTTGGCTGTGATTCCATGAGAGAGGCAATGTCATTGTTTGATGAAATGGTGGTGAAAGATATAAGAAAACTGACACGAGTTACACTGGGGAGCATACTCCGAGCTTGCACAAAGCTAACTGATCTGAGTAGAGGGAGACGAGTACATTGTTATGCAATTAAAGCCGGTTTTGATTTAAATACAATGGTGGGCAACACCATCCTTTCTATGTATGCAAAGTGTGGGATTATAGATGATGCATTCAGGTTATTTGATATAATGGATCCAAAAGACACGGTTTCTTATGGGGCTATCATCTCAGGTTGTGTGCAGAATGGTTATGCAAAGGAAGCTTTGCTTATATTCTACCAAATGCAGTTGTCTGGGATTGATCCAGATTTGGCAACCATGGCAGGTGTTTTGCCGGCTTGTTCACATTTGGCAGCCCTGCAACATGGGGCCTGTGGCCATGGTTATTCAATTGTTCATGGCTTTGCTACAGACACCTCAATTTGTAATGCCTTAATAGACATGTACTCGAAATGTGGGAAGATCCATATAGGCAGGCAAGTATTCGACAGAATGCTTAAGCGGGATACTGTCTCATGGAACGCAATGATAGTTGGTTATGGGATTCATGGCCTTGGGATGGAAGCAATTTCACAGTTCCACAATATGCAGGCAGCTGGAATAAAGCCCGACGATGTGACTTTCATTGGCCTCTTATCTGCTTGCAGTCATTCGGGGCTTGTCACTCAAGGAAAACACTGGTTTAGTGCTATGAGTCAAGATTTCAGCATAACCCCAAGGATGGAACACTACATATGCATGGTGGATCTTCTTGCCCGAACTGGACTGTTGGCCGAGGCGCACAGTTTCATTCAAAGCATGCCATTTGAGGCTGATGTTCGAGTATGGAGTGCACTACTTGCTGCATGTAGGGTTCACAATAATATTGAACTTGGGGAAGAAGTGTCCAGGAAAGTTCAAGGGCTAGGATCTGAAGGTACCGGAAATTTGGTCCTCTTATCTAATATGTACAGTTCTATTGGGAGATGGGATGATGCAGCGCATGTCAGAATTAAACAGAAGGATCAAGGCCTTAAGAAGAGTCCAGGATGTAGTTGGGTCGAGATTAATGGGGTGATTCATGGATTTCTCGGTGGAGATCAGTCCCATCCTGAATCGGCTCAGATACATGAAAAATTAGAGGAACTGCTAGTAGACATGAAAAAGATGGGATATTGTGCAGAAAATAGTTTTGTTCTCCAAGATGTTGAGGAAGAGGAGAAAGAAAGGATCCTCCTTTATCATAGTGAAAAACTAGCCATTGCTTATGGAACTCTTCATCTGAGCCCGGGGAAACCCGTTTTTGTTACTAAAAATCTGCGGGTCTGTGGTGATTGCCATGCTGCTATAAAGTTCATCTCTCTTGTTACAAAGAGAGAGATAACCGTAAGAGATGTGAGTCGGTTTCATCATTTCAGTGATGGAAATTGCAGTTGTGCAGACTTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

808

Amino Acids

89.71

Weight (kDa)

6.99

Isoelectric Point (pI)

40.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	94 - 141	2.3e-10	PPR repeat family
PPR_2	PF13041	298 - 346	7.9e-06	PPR repeat family
PPR_2	PF13041	399 - 446	1.8e-06	PPR repeat family
PPR_2	PF13041	500 - 548	1.3e-09	PPR repeat family
E_motif	PF20431	618 - 680	5.5e-18	E motif
Eplus_motif	PF20430	685 - 709	2.6e-07	E+ motif
DYW_deaminase	PF14432	716 - 808	1.1e-38	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011260)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16610
fragaria_vesca	FvH4_5g18140 FvH4_5g18140
malus_domestica	MD14G1243800.v1.1
prunus_persica	Prupe.5G241400_v2.0.a1 Prupe.5G241400_v2.0.a1
pyrus_communis	pycom14g20490
rosa_chinensis	RchiOBHm_Chr7g0177471
rosa_laevigata	RLG00000005535
rosa_multiflora	Rmu_co8313469.1_g000001 Rmu_sc0036713.1_g000001 Rmu_sc0036714.1_g000001
rosa_roxburghii	Rroxscaffold_3G00275850
rosa_rugosa	Rorug06G0406200 Rorug06G0406300
rosa_samantha	Rh7AG005800 Rh7BG005700 Rh7CG006100 Rh7DG005600
rosa_wichuraiana	Rw7G000470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	164
AarI	CACCTGC	1 cut(s)	1310
AatII	GACGTC	1 cut(s)	498
Acc16I	TGCGCA	2 cut(s)	555, 729
Acc36I	ACCTGC	1 cut(s)	1310
Acc65I	GGTACC	1 cut(s)	1913
AccB1I	GGYRCC	1 cut(s)	1913
AccBSI	CCGCTC	1 cut(s)	677
AccI	GTMKAC	1 cut(s)	2130
AccII	CGCG	3 cut(s)	48, 740, 860
AciI	CCGC	5 cut(s)	12, 677, 710, 1498, 2296
AclWI	GGATC	9 cut(s)	1184, 1197, 1292, 1453, 1749, 1912, 2007, 2200, 2213
AcoI	YGGCCR	2 cut(s)	1372, 1767
AcsI	RAATTY	2 cut(s)	270, 1921
AcuI	CTGAAG	1 cut(s)	1929
AcyI	GRCGYC	1 cut(s)	495
AfiI	CCNNNNNNNGG	6 cut(s)	184, 321, 754, 780, 1555, 1717
AflII	CTTAAG	2 cut(s)	1493, 2009
AflIII	ACRYGT	3 cut(s)	396, 442, 1437
AgeI	ACCGGT	1 cut(s)	189
AgsI	TTSAA	8 cut(s)	107, 206, 338, 665, 1386, 1790, 1865, 1895
AjiI	CACGTC	1 cut(s)	802
AjnI	CCWGG	2 cut(s)	1883, 2020
AluBI	AGCT	6 cut(s)	551, 734, 1031, 1042, 1259, 1592
AluI	AGCT	6 cut(s)	551, 734, 1031, 1042, 1259, 1592
Alw21I	GWGCWC	1 cut(s)	1831
Alw26I	GTCTC	4 cut(s)	198, 534, 1057, 1513
Alw44I	GTGCAC	1 cut(s)	1827
AlwI	GGATC	9 cut(s)	1184, 1197, 1292, 1453, 1749, 1912, 2007, 2200, 2213
Ama87I	CYCGRG	2 cut(s)	424, 2263
AoxI	GGCC	8 cut(s)	54, 756, 1365, 1372, 1546, 1624, 1767, 2005
ApaLI	GTGCAC	1 cut(s)	1827
ApeKI	GCWGC	6 cut(s)	551, 1349, 1589, 1838, 1973, 2318
ApoI	RAATTY	2 cut(s)	270, 1921
AseI	ATTAAT	1 cut(s)	2043
AsiGI	ACCGGT	1 cut(s)	189
Asp718I	GGTACC	1 cut(s)	1913
AspLEI	GCGC	8 cut(s)	50, 556, 730, 742, 820, 923, 1777, 1978
AspS9I	GGNCC	4 cut(s)	187, 329, 1365, 1927
AsuC2I	CCSGG	3 cut(s)	217, 2264, 2265
AsuHPI	GGTGA	4 cut(s)	653, 991, 2062, 2318
AvaI	CYCGRG	2 cut(s)	424, 2263
AvaII	GGWCC	3 cut(s)	187, 329, 1927
BaeGI	GKGCMC	1 cut(s)	1831
BalI	TGGCCA	1 cut(s)	1374
BamHI	GGATCC	2 cut(s)	1189, 2205
BanI	GGYRCC	1 cut(s)	1913
BanII	GRGCYC	2 cut(s)	486, 2264
BauI	CACGAG	1 cut(s)	1002
Bbv12I	GWGCWC	1 cut(s)	1831
BbvI	GCAGC	6 cut(s)	538, 1361, 1601, 1825, 1985, 2305
BccI	CCATC	9 cut(s)	159, 563, 1133, 1549, 1711, 1956, 2091, 2137, 2390
BciT130I	CCWGG	2 cut(s)	1885, 2022
BciVI	GTATCC	1 cut(s)	1495
BcnI	CCSGG	3 cut(s)	217, 2264, 2265
BcoDI	GTCTC	4 cut(s)	198, 534, 1057, 1513
BfaI	CTAG	4 cut(s)	212, 1901, 2126, 2231
BfmI	CTRYAG	2 cut(s)	648, 1404
BfrI	CTTAAG	2 cut(s)	1493, 2009
BfuAI	ACCTGC	1 cut(s)	1310
BfuI	GTATCC	1 cut(s)	1495
BisI	GCNGC	6 cut(s)	552, 1350, 1590, 1839, 1974, 2319
BlpI	GCTNAGC	1 cut(s)	743
BlsI	GCNGC	6 cut(s)	553, 1351, 1591, 1840, 1975, 2320
BmcAI	AGTACT	1 cut(s)	814
Bme1390I	CCNGG	5 cut(s)	217, 1885, 2022, 2264, 2265
Bme18I	GGWCC	3 cut(s)	187, 329, 1927
BmeT110I	CYCGRG	2 cut(s)	424, 2263
BmgBI	CACGTC	1 cut(s)	802
BmgT120I	GGNCC	4 cut(s)	187, 329, 1365, 1927
BmiI	GGNNCC	5 cut(s)	299, 1191, 1366, 1915, 2207
BmrFI	CCNGG	5 cut(s)	217, 1885, 2022, 2264, 2265
BmrI	ACTGGG	2 cut(s)	383, 1022
BmsI	GCATC	3 cut(s)	865, 1153, 1960
BmuI	ACTGGG	2 cut(s)	383, 1022
Bpu1102I	GCTNAGC	1 cut(s)	743
BpuEI	CTTGAG	3 cut(s)	120, 398, 1647
BpuMI	CCSGG	3 cut(s)	217, 2264, 2265
BsaAI	YACGTR	1 cut(s)	399
BsaBI	GATNNNNATC	1 cut(s)	124
BsaHI	GRCGYC	1 cut(s)	495
BsaI	GGTCTC	1 cut(s)	198
BsaJI	CCNNGG	8 cut(s)	748, 1314, 1375, 1549, 1712, 1770, 2263, 2264
BsaWI	WCCGGW	2 cut(s)	189, 1916
BsaXI	ACNNNNNCTCC	4 cut(s)	531, 561, 775, 805
Bsc4I	CCNNNNNNNGG	6 cut(s)	184, 321, 754, 780, 1555, 1717
Bse118I	RCCGGY	3 cut(s)	189, 1091, 1330
Bse1I	ACTGG	4 cut(s)	378, 1017, 1679, 1762
Bse3DI	GCAATG	4 cut(s)	885, 960, 1527, 2235
Bse8I	GATNNNNATC	1 cut(s)	124
BseBI	CCWGG	2 cut(s)	1885, 2022
BseDI	CCNNGG	8 cut(s)	748, 1314, 1375, 1549, 1712, 1770, 2263, 2264
BseGI	GGATG	6 cut(s)	1125, 1560, 1722, 1972, 2030, 2083
BseJI	GATNNNNATC	1 cut(s)	124
BseLI	CCNNNNNNNGG	6 cut(s)	184, 321, 754, 780, 1555, 1717
BseMI	GCAATG	4 cut(s)	885, 960, 1527, 2235
BseMII	CTCAG	5 cut(s)	734, 1043, 1239, 2111, 2249
BseNI	ACTGG	4 cut(s)	378, 1017, 1679, 1762
BseRI	GAGGAG	4 cut(s)	793, 796, 877, 2207
BseSI	GKGCMC	1 cut(s)	1831
BseXI	GCAGC	6 cut(s)	538, 1361, 1601, 1825, 1985, 2305
BseYI	CCCAGC	1 cut(s)	614
BsgI	GTGCAG	2 cut(s)	1256, 2175
Bsh1236I	CGCG	3 cut(s)	48, 740, 860
Bsh1285I	CGRYCG	1 cut(s)	16
BshFI	GGCC	8 cut(s)	56, 758, 1367, 1374, 1548, 1626, 1769, 2007
BshNI	GGYRCC	1 cut(s)	1913
BshTI	ACCGGT	1 cut(s)	189
BsiEI	CGRYCG	1 cut(s)	16
BsiHKAI	GWGCWC	1 cut(s)	1831
BsiHKCI	CYCGRG	2 cut(s)	424, 2263
BsiSI	CCGG	6 cut(s)	190, 217, 1092, 1331, 1917, 2264
BslFI	GGGAC	1 cut(s)	2065
BslI	CCNNNNNNNGG	6 cut(s)	184, 321, 754, 780, 1555, 1717
BsmAI	GTCTC	4 cut(s)	198, 534, 1057, 1513
BsmBI	CGTCTC	1 cut(s)	1057
BsmFI	GGGAC	1 cut(s)	2065
BsmI	GAATGC	5 cut(s)	114, 607, 697, 1166, 1494
BsnI	GGCC	8 cut(s)	56, 758, 1367, 1374, 1548, 1626, 1769, 2007
Bso31I	GGTCTC	1 cut(s)	198
BsoBI	CYCGRG	2 cut(s)	424, 2263
Bsp1286I	GDGCHC	3 cut(s)	486, 1831, 2264
Bsp1407I	TGTACA	1 cut(s)	1944
Bsp1720I	GCTNAGC	1 cut(s)	743
Bsp19I	CCATGG	2 cut(s)	1314, 1375
BspACI	CCGC	5 cut(s)	12, 677, 710, 1498, 2296
BspANI	GGCC	8 cut(s)	56, 758, 1367, 1374, 1548, 1626, 1769, 2007
BspCNI	CTCAG	5 cut(s)	735, 1044, 1238, 2110, 2250
BspFNI	CGCG	3 cut(s)	48, 740, 860
BspLI	GGNNCC	5 cut(s)	299, 1191, 1366, 1915, 2207
BspMAI	CTGCAG	1 cut(s)	652
BspMI	ACCTGC	1 cut(s)	1310
BspPI	GGATC	9 cut(s)	1184, 1197, 1292, 1453, 1749, 1912, 2007, 2200, 2213
BspT107I	GGYRCC	1 cut(s)	1913
BspTI	CTTAAG	2 cut(s)	1493, 2009
BspTNI	GGTCTC	1 cut(s)	198
BsrBI	CCGCTC	1 cut(s)	677
BsrDI	GCAATG	4 cut(s)	885, 960, 1527, 2235
BsrFI	RCCGGY	3 cut(s)	189, 1091, 1330
BsrGI	TGTACA	1 cut(s)	1944
BsrI	ACTGG	4 cut(s)	378, 1017, 1679, 1762
BssAI	RCCGGY	3 cut(s)	189, 1091, 1330
BssECI	CCNNGG	8 cut(s)	748, 1314, 1375, 1549, 1712, 1770, 2263, 2264
BssNI	GRCGYC	1 cut(s)	495
BssSI	CACGAG	1 cut(s)	1002
BssT1I	CCWWGG	5 cut(s)	748, 1314, 1375, 1549, 1712
Bst2BI	CACGAG	1 cut(s)	1002
Bst2UI	CCWGG	2 cut(s)	1885, 2022
Bst6I	CTCTTC	5 cut(s)	78, 201, 2009, 2184, 2256
BstACI	GRCGYC	1 cut(s)	495
BstAFI	CTTAAG	2 cut(s)	1493, 2009
BstAPI	GCANNNNNTGC	1 cut(s)	1835
BstAUI	TGTACA	1 cut(s)	1944
BstBAI	YACGTR	1 cut(s)	399
BstDEI	CTNAG	6 cut(s)	743, 809, 1052, 1225, 2097, 2258
BstDSI	CCRYGG	2 cut(s)	1314, 1375
BstF5I	GGATG	6 cut(s)	1125, 1560, 1722, 1972, 2030, 2083
BstFNI	CGCG	3 cut(s)	48, 740, 860
BstHHI	GCGC	8 cut(s)	50, 556, 730, 742, 820, 923, 1777, 1978
BstMAI	GTCTC	4 cut(s)	198, 534, 1057, 1513
BstMCI	CGRYCG	1 cut(s)	16
BstMWI	GCNNNNNNNGC	9 cut(s)	481, 602, 611, 683, 692, 1088, 1256, 1316, 1835
BstNI	CCWGG	2 cut(s)	1885, 2022
BstNSI	RCATGY	7 cut(s)	367, 423, 470, 1441, 1798, 1845, 1982
BstSCI	CCNGG	5 cut(s)	215, 1883, 2020, 2262, 2263
BstSFI	CTRYAG	2 cut(s)	648, 1404
BstSLI	GKGCMC	1 cut(s)	1831
BstUI	CGCG	3 cut(s)	48, 740, 860
BstV1I	GCAGC	6 cut(s)	538, 1361, 1601, 1825, 1985, 2305
BstX2I	RGATCY	5 cut(s)	1189, 1458, 1741, 1904, 2205
BstYI	RGATCY	5 cut(s)	1189, 1458, 1741, 1904, 2205
BsuI	GTATCC	1 cut(s)	1495
BsuRI	GGCC	8 cut(s)	56, 758, 1367, 1374, 1548, 1626, 1769, 2007
BtgI	CCRYGG	2 cut(s)	1314, 1375
BtgZI	GCGATG	1 cut(s)	926
BtrI	CACGTC	1 cut(s)	802
BtsCI	GGATG	6 cut(s)	1125, 1560, 1722, 1972, 2030, 2083
BtsI	GCAGTG	2 cut(s)	507, 645
BtsIMutI	CAGTG	6 cut(s)	507, 645, 851, 1010, 1672, 2398
BveI	ACCTGC	1 cut(s)	1310
CfoI	GCGC	8 cut(s)	50, 556, 730, 742, 820, 923, 1777, 1978
Cfr10I	RCCGGY	3 cut(s)	189, 1091, 1330
Cfr13I	GGNCC	4 cut(s)	187, 329, 1365, 1927
Cfr9I	CCCGGG	1 cut(s)	2263
CpoI	CGGWCCG	1 cut(s)	329
CspAI	ACCGGT	1 cut(s)	189
CspI	CGGWCCG	1 cut(s)	329
DdeI	CTNAG	6 cut(s)	743, 809, 1052, 1225, 2097, 2258
DraI	TTTAAA	1 cut(s)	1104
EaeI	YGGCCR	2 cut(s)	1372, 1767
Eam1104I	CTCTTC	5 cut(s)	78, 201, 2009, 2184, 2256
EarI	CTCTTC	5 cut(s)	78, 201, 2009, 2184, 2256
Eco130I	CCWWGG	5 cut(s)	748, 1314, 1375, 1549, 1712
Eco147I	AGGCCT	2 cut(s)	56, 2007
Eco24I	GRGCYC	2 cut(s)	486, 2264
Eco31I	GGTCTC	1 cut(s)	198
Eco47I	GGWCC	3 cut(s)	187, 329, 1927
Eco57I	CTGAAG	1 cut(s)	1929
Eco88I	CYCGRG	2 cut(s)	424, 2263
EcoO109I	RGGNCCY	1 cut(s)	1365
EcoRII	CCWGG	2 cut(s)	1883, 2020
EcoT14I	CCWWGG	5 cut(s)	748, 1314, 1375, 1549, 1712
EcoT22I	ATGCAT	3 cut(s)	322, 1168, 1736
EcoT38I	GRGCYC	2 cut(s)	486, 2264
ErhI	CCWWGG	5 cut(s)	748, 1314, 1375, 1549, 1712
Esp3I	CGTCTC	1 cut(s)	1057
FalI	AAGNNNNNCTT	2 cut(s)	1248, 1280
FaqI	GGGAC	1 cut(s)	2065
FauI	CCCGC	3 cut(s)	670, 1491, 2289
FauNDI	CATATG	2 cut(s)	925, 1730
FblI	GTMKAC	1 cut(s)	2130
Fnu4HI	GCNGC	6 cut(s)	552, 1350, 1590, 1839, 1974, 2319
FokI	GGATG	6 cut(s)	1112, 1567, 1729, 1979, 2037, 2070
FriOI	GRGCYC	2 cut(s)	486, 2264
Fsp4HI	GCNGC	6 cut(s)	552, 1350, 1590, 1839, 1974, 2319
FspBI	CTAG	4 cut(s)	212, 1901, 2126, 2231
FspI	TGCGCA	2 cut(s)	555, 729
GlaI	GCGC	8 cut(s)	49, 555, 729, 741, 819, 922, 1776, 1977
GluI	GCNGC	6 cut(s)	552, 1350, 1590, 1839, 1974, 2319
GsaI	CCCAGC	1 cut(s)	618
HaeIII	GGCC	8 cut(s)	56, 758, 1367, 1374, 1548, 1626, 1769, 2007
HapII	CCGG	6 cut(s)	190, 217, 1092, 1331, 1917, 2264
HhaI	GCGC	8 cut(s)	50, 556, 730, 742, 820, 923, 1777, 1978
Hin1I	GRCGYC	1 cut(s)	495
Hin6I	GCGC	8 cut(s)	48, 554, 728, 740, 818, 921, 1775, 1976
HinP1I	GCGC	8 cut(s)	48, 554, 728, 740, 818, 921, 1775, 1976
HindIII	AAGCTT	1 cut(s)	1257
HinfI	GANTC	8 cut(s)	867, 938, 1540, 1690, 2017, 2053, 2090, 2374
HpaII	CCGG	6 cut(s)	190, 217, 1092, 1331, 1917, 2264
HphI	GGTGA	4 cut(s)	653, 991, 2062, 2318
Hpy166II	GTNNAC	6 cut(s)	396, 856, 1340, 1829, 1852, 2131
Hpy188I	TCNGA	8 cut(s)	38, 493, 1028, 1053, 1909, 1985, 2100, 2259
Hpy188III	TCNNGA	6 cut(s)	452, 889, 1301, 1694, 2038, 2087
Hpy8I	GTNNAC	6 cut(s)	396, 856, 1340, 1829, 1852, 2131
Hpy99I	CGWCG	2 cut(s)	269, 1610
HpyAV	CCTTC	3 cut(s)	748, 1904, 1990
HpyCH4IV	ACGT	4 cut(s)	398, 444, 495, 801
HpyF10VI	GCNNNNNNNGC	9 cut(s)	481, 602, 611, 683, 692, 1088, 1256, 1316, 1835
HpyF3I	CTNAG	6 cut(s)	743, 809, 1052, 1225, 2097, 2258
HpySE526I	ACGT	4 cut(s)	398, 444, 495, 801
Hsp92I	GRCGYC	1 cut(s)	495
HspAI	GCGC	8 cut(s)	48, 554, 728, 740, 818, 921, 1775, 1976
KpnI	GGTACC	1 cut(s)	1917
KroI	GCCGGC	1 cut(s)	1330
KroNI	GCCGGC	1 cut(s)	1332
LmnI	GCTCC	1 cut(s)	1017
Lsp1109I	GCAGC	6 cut(s)	538, 1361, 1601, 1825, 1985, 2305
LweI	GCATC	3 cut(s)	865, 1153, 1960
MaeI	CTAG	4 cut(s)	212, 1901, 2126, 2231
MaeII	ACGT	4 cut(s)	398, 444, 495, 801
MaeIII	GTNAC	8 cut(s)	86, 641, 767, 1006, 1612, 1657, 2281, 2341
MbiI	CCGCTC	1 cut(s)	677
MboII	GAAGA	9 cut(s)	65, 188, 707, 1468, 1736, 1886, 2026, 2201, 2243
MfeI	CAATTG	1 cut(s)	1386
MflI	RGATCY	5 cut(s)	1189, 1458, 1741, 1904, 2205
MhlI	GDGCHC	3 cut(s)	486, 1831, 2264
MlsI	TGGCCA	1 cut(s)	1374
MluNI	TGGCCA	1 cut(s)	1374
MlyI	GAGTC	3 cut(s)	1699, 2026, 2383
MmeI	TCCRAC	1 cut(s)	884
Mox20I	TGGCCA	1 cut(s)	1374
Mph1103I	ATGCAT	3 cut(s)	322, 1168, 1736
MroNI	GCCGGC	1 cut(s)	1330
MscI	TGGCCA	1 cut(s)	1374
MseI	TTAA	8 cut(s)	381, 1086, 1103, 1430, 1494, 1989, 2010, 2043
MslI	CAYNNNNRTG	4 cut(s)	465, 1077, 1733, 2391
Msp20I	TGGCCA	1 cut(s)	1374
MspA1I	CMGCKG	1 cut(s)	1592
MspCI	CTTAAG	2 cut(s)	1493, 2009
MspI	CCGG	6 cut(s)	190, 217, 1092, 1331, 1917, 2264
MspR9I	CCNGG	5 cut(s)	217, 1885, 2022, 2264, 2265
MunI	CAATTG	1 cut(s)	1386
Mva1269I	GAATGC	5 cut(s)	114, 607, 697, 1166, 1494
MvaI	CCWGG	2 cut(s)	1885, 2022
MvnI	CGCG	3 cut(s)	48, 740, 860
MwoI	GCNNNNNNNGC	9 cut(s)	481, 602, 611, 683, 692, 1088, 1256, 1316, 1835
NaeI	GCCGGC	1 cut(s)	1332
NciI	CCSGG	3 cut(s)	217, 2264, 2265
NcoI	CCATGG	2 cut(s)	1314, 1375
NdeI	CATATG	2 cut(s)	925, 1730
NgoMIV	GCCGGC	1 cut(s)	1330
NlaIV	GGNNCC	5 cut(s)	299, 1191, 1366, 1915, 2207
NmeAIII	GCCGAG	3 cut(s)	281, 465, 1795
NmuCI	GTSAC	3 cut(s)	641, 1612, 1657
NsbI	TGCGCA	2 cut(s)	555, 729
NsiI	ATGCAT	3 cut(s)	322, 1168, 1736
NspI	RCATGY	7 cut(s)	367, 423, 470, 1441, 1798, 1845, 1982
PaeI	GCATGC	3 cut(s)	367, 423, 1798
PaqCI	CACCTGC	1 cut(s)	1310
PceI	AGGCCT	2 cut(s)	56, 2007
PciI	ACATGT	1 cut(s)	1437
PcsI	WCGNNNNNNNCGW	2 cut(s)	375, 492
PctI	GAATGC	5 cut(s)	114, 607, 697, 1166, 1494
PdiI	GCCGGC	1 cut(s)	1332
PfeI	GAWTC	5 cut(s)	867, 938, 1540, 2053, 2090
PfoI	TCCNGGA	2 cut(s)	1883, 2020
PinAI	ACCGGT	1 cut(s)	189
PkrI	GCNGC	6 cut(s)	553, 1351, 1591, 1840, 1975, 2320
PleI	GAGTC	3 cut(s)	1698, 2025, 2382
PpsI	GAGTC	3 cut(s)	1698, 2025, 2382
Ppu21I	YACGTR	1 cut(s)	399
PscI	ACATGT	1 cut(s)	1437
PshBI	ATTAAT	1 cut(s)	2043
PsiI	TTATAA	1 cut(s)	164
Psp6I	CCWGG	2 cut(s)	1883, 2020
PspFI	CCCAGC	1 cut(s)	614
PspGI	CCWGG	2 cut(s)	1883, 2020
PspN4I	GGNNCC	5 cut(s)	299, 1191, 1366, 1915, 2207
PspPI	GGNCC	4 cut(s)	187, 329, 1365, 1927
PstI	CTGCAG	1 cut(s)	652
PsuI	RGATCY	5 cut(s)	1189, 1458, 1741, 1904, 2205
PvuII	CAGCTG	1 cut(s)	1592
RseI	CAYNNNNRTG	4 cut(s)	465, 1077, 1733, 2391
Rsr2I	CGGWCCG	1 cut(s)	329
RsrII	CGGWCCG	1 cut(s)	329
SaqAI	TTAA	8 cut(s)	381, 1086, 1103, 1430, 1494, 1989, 2010, 2043
SatI	GCNGC	6 cut(s)	552, 1350, 1590, 1839, 1974, 2319
Sau96I	GGNCC	4 cut(s)	187, 329, 1365, 1927
ScaI	AGTACT	1 cut(s)	814
SchI	GAGTC	3 cut(s)	1699, 2026, 2383
ScrFI	CCNGG	5 cut(s)	217, 1885, 2022, 2264, 2265
SduI	GDGCHC	3 cut(s)	486, 1831, 2264
SfaNI	GCATC	3 cut(s)	865, 1153, 1960
SfcI	CTRYAG	2 cut(s)	648, 1404
SinI	GGWCC	3 cut(s)	187, 329, 1927
SmaI	CCCGGG	1 cut(s)	2265
SmiI	ATTTAAAT	1 cut(s)	1104
SmiMI	CAYNNNNRTG	4 cut(s)	465, 1077, 1733, 2391
SmlI	CTYRAG	5 cut(s)	135, 413, 1493, 1662, 2009
SmoI	CTYRAG	5 cut(s)	135, 413, 1493, 1662, 2009
SphI	GCATGC	3 cut(s)	367, 423, 1798
SseBI	AGGCCT	2 cut(s)	56, 2007
SsiI	CCGC	5 cut(s)	12, 677, 710, 1498, 2296
SspI	AATATT	1 cut(s)	1861
SspMI	CTAG	4 cut(s)	212, 1901, 2126, 2231
StuI	AGGCCT	2 cut(s)	56, 2007
StyD4I	CCNGG	5 cut(s)	215, 1883, 2020, 2262, 2263
StyI	CCWWGG	5 cut(s)	748, 1314, 1375, 1549, 1712
SwaI	ATTTAAAT	1 cut(s)	1104
TaiI	ACGT	4 cut(s)	401, 447, 498, 804
TaqI	TCGA	8 cut(s)	118, 183, 264, 369, 1445, 1482, 1816, 2037
TatI	WGTACW	6 cut(s)	354, 393, 812, 1069, 1440, 1944
TfiI	GAWTC	5 cut(s)	867, 938, 1540, 2053, 2090
Tru1I	TTAA	8 cut(s)	381, 1086, 1103, 1430, 1494, 1989, 2010, 2043
Tru9I	TTAA	8 cut(s)	381, 1086, 1103, 1430, 1494, 1989, 2010, 2043
TscAI	CASTG	6 cut(s)	514, 652, 851, 1017, 1679, 2398
TseFI	GTSAC	3 cut(s)	641, 1612, 1657
TseI	GCWGC	6 cut(s)	551, 1349, 1589, 1838, 1973, 2318
Tsp45I	GTSAC	3 cut(s)	641, 1612, 1657
TspGWI	ACGGA	1 cut(s)	342
TspMI	CCCGGG	1 cut(s)	2263
TspRI	CASTG	6 cut(s)	514, 652, 851, 1017, 1679, 2398
Vha464I	CTTAAG	2 cut(s)	1493, 2009
VneI	GTGCAC	1 cut(s)	1827
VpaK11BI	GGWCC	3 cut(s)	187, 329, 1927
VspI	ATTAAT	1 cut(s)	2043
XapI	RAATTY	2 cut(s)	270, 1921
XceI	RCATGY	7 cut(s)	367, 423, 470, 1441, 1798, 1845, 1982
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2028
XmaI	CCCGGG	1 cut(s)	2263
XmiI	GTMKAC	1 cut(s)	2130
XspI	CTAG	4 cut(s)	212, 1901, 2126, 2231
ZraI	GACGTC	1 cut(s)	496
ZrmI	AGTACT	1 cut(s)	814
Zsp2I	ATGCAT	3 cut(s)	322, 1168, 1736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.