Rorug06G0406300

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011260
Physical Location & Seq
Forward (+)
54991642 .. 54999221
7580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0406300.1

Sequence Viewer

Length: 882 bp
ATGGCAACCACTAGAATATTGCGCACGTTTAAGCCTATGACTATTCATTCCTCCTCTGCTTCTTCTTCTTCTTCACCACCAACAACTGTTAGGTATCTGAGCAAGGATGCCGGGTTGATGAGTGGAGAGAAAAAACTTGGTACTGCGAATGAGAGAAAACAAATGGCGGTTGTTAAGGCTTCAGTGGCCGGTTCCCAGACACTTACCACATCGAAGCCACAAGTGAATCGAGGAGTTATAGATTTGGCTTCCTTGGTTACAAAGACCAATCGTTCTTTGCTCATTCTACTAAGAACGGCTCTCAAGAGAAAATCTATACAGCAGCAGGTCCAGATGTTCATTGAAAGGGGCATAATTGACTGTCGGTTCTTTACATTACTTGCTGTTGCAGGATCTTTACTTGGTTCAGTACTGTGCTTTGTCGAGGGTTGTTTTATTGTTCTAGAGTCATATTTCCAGTATTTTCATGCCTTGTCTCAGAAGACAGATCAAGGACATATGGTGCACCTACTTATTGAAGCAATAGACATGTTCCTAGTAGGAACAGCCATGCTTATATTCGGAGTTGGGTTGTATGCCATGTTCATGGGGTCAAAGGCTGCGAAAGATAGAGGACCGCGGTTCAGTGAGTCAAACTTGTTTGGGCTGTTCTACATGAAGGCAGCTCCAGCATGGGTTGACATGAAATCAGTTTCGCAAGCAAAATCTAAAATTGGGCATGCAGTGGTGATGATACTTCAAGTGGGACTGTTGGAGAAGTTCAAAAGTATACCTCTTGTCACACCACTCGATCTTGCTTGCTTTGCCGGAGCTGTGCTCGTCTCCTCAGCTTGTATCTTCCTTCTTTCCAGACTTGATTATTCTGCTACCACTGCTGGCTGA

Protein Analysis

293

Amino Acids

31.81

Weight (kDa)

9.8

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 118 - 254 3.6e-33 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 23
Acc36I ACCTGC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 769
AccII CGCG 1 cut(s) 619
AciI CCGC 3 cut(s) 167, 617, 619
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 186
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 2 cut(s) 142, 411
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 4 cut(s) 344, 518, 740, 763
AluBI AGCT 3 cut(s) 665, 812, 830
AluI AGCT 3 cut(s) 665, 812, 830
Alw21I GWGCWC 2 cut(s) 507, 819
Alw26I GTCTC 2 cut(s) 480, 826
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 1 cut(s) 400
AoxI GGCC 1 cut(s) 186
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 3 cut(s) 322, 599, 662
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 2 cut(s) 328, 614
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 2 cut(s) 66, 739
AvaII GGWCC 2 cut(s) 328, 614
BaeGI GKGCMC 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 507, 819
BbvCI CCTCAGC 1 cut(s) 826
BbvI GCAGC 3 cut(s) 334, 586, 674
BceAI ACGGC 1 cut(s) 312
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 480, 826
BfaI CTAG 3 cut(s) 12, 443, 536
BfuAI ACCTGC 1 cut(s) 316
BisI GCNGC 3 cut(s) 323, 600, 663
BlsI GCNGC 3 cut(s) 324, 601, 664
BmcAI AGTACT 1 cut(s) 411
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 2 cut(s) 328, 614
BmgT120I GGNCC 2 cut(s) 328, 614
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 488
BplI GAGNNNNNCTC 2 cut(s) 801, 833
BpmI CTGGAG 1 cut(s) 651
Bpu10I CCTNAGC 1 cut(s) 826
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 252, 617
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 457
BseDI CCNNGG 2 cut(s) 252, 617
BseGI GGATG 1 cut(s) 112
BseMII CTCAG 3 cut(s) 89, 491, 840
BseNI ACTGG 1 cut(s) 457
BseRI GAGGAG 3 cut(s) 43, 246, 814
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 3 cut(s) 334, 586, 674
Bsh1236I CGCG 1 cut(s) 619
BshFI GGCC 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 507, 819
BsiSI CCGG 3 cut(s) 111, 189, 807
BslFI GGGAC 1 cut(s) 759
BsmAI GTCTC 2 cut(s) 480, 826
BsmBI CGTCTC 1 cut(s) 826
BsmFI GGGAC 1 cut(s) 759
BsnI GGCC 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 507, 819
Bsp143I GATC 3 cut(s) 392, 487, 790
BspACI CCGC 3 cut(s) 167, 617, 619
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 3 cut(s) 90, 490, 839
BspFNI CGCG 1 cut(s) 619
BspLI GGNNCC 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 1 cut(s) 400
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 1 cut(s) 457
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 2 cut(s) 252, 617
BssMI GATC 3 cut(s) 392, 487, 790
BssNAI GTATAC 1 cut(s) 770
BssT1I CCWWGG 1 cut(s) 252
Bst1107I GTATAC 1 cut(s) 770
Bst4CI ACNGT 4 cut(s) 88, 362, 414, 750
BstC8I GCNNGC 4 cut(s) 699, 720, 799, 877
BstDEI CTNAG 4 cut(s) 98, 290, 477, 826
BstDSI CCRYGG 1 cut(s) 617
BstF5I GGATG 1 cut(s) 112
BstFNI CGCG 1 cut(s) 619
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 3 cut(s) 395, 490, 793
BstMAI GTCTC 2 cut(s) 480, 826
BstMBI GATC 3 cut(s) 392, 487, 790
BstMWI GCNNNNNNNGC 4 cut(s) 185, 668, 803, 872
BstNSI RCATGY 2 cut(s) 532, 722
BstSCI CCNGG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 507
BstUI CGCG 1 cut(s) 619
BstV1I GCAGC 3 cut(s) 334, 586, 674
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 1 cut(s) 392
BstZ17I GTATAC 1 cut(s) 770
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 617
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 2 cut(s) 729, 870
BtsIMutI CAGTG 4 cut(s) 189, 631, 729, 870
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 4 cut(s) 699, 720, 799, 877
CfoI GCGC 1 cut(s) 24
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 328, 614
Cfr42I CCGCGG 1 cut(s) 620
Csp6I GTAC 2 cut(s) 141, 410
CviAII CATG 9 cut(s) 467, 529, 550, 580, 586, 655, 672, 682, 719
CviQI GTAC 2 cut(s) 141, 410
DdeI CTNAG 4 cut(s) 98, 290, 477, 826
DpnI GATC 3 cut(s) 394, 489, 792
DpnII GATC 3 cut(s) 392, 487, 790
EaeI YGGCCR 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 328, 614
Eco57I CTGAAG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 826
FaeI CATG 9 cut(s) 470, 532, 553, 583, 589, 658, 675, 685, 722
FaqI GGGAC 1 cut(s) 759
FatI CATG 9 cut(s) 466, 528, 549, 579, 585, 654, 671, 681, 718
FauNDI CATATG 1 cut(s) 498
FblI GTMKAC 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 323, 600, 663
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 3 cut(s) 323, 600, 663
FspBI CTAG 3 cut(s) 12, 443, 536
FspI TGCGCA 1 cut(s) 23
GlaI GCGC 1 cut(s) 23
GluI GCNGC 3 cut(s) 323, 600, 663
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 1 cut(s) 188
HapII CCGG 3 cut(s) 111, 189, 807
HhaI GCGC 1 cut(s) 24
Hin1II CATG 9 cut(s) 470, 532, 553, 583, 589, 658, 675, 685, 722
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HinfI GANTC 3 cut(s) 226, 446, 629
HpaII CCGG 3 cut(s) 111, 189, 807
HphI GGTGA 2 cut(s) 66, 739
Hpy166II GTNNAC 3 cut(s) 505, 679, 770
Hpy188I TCNGA 3 cut(s) 99, 480, 563
Hpy188III TCNNGA 4 cut(s) 304, 331, 443, 849
Hpy8I GTNNAC 3 cut(s) 505, 679, 770
HpyAV CCTTC 2 cut(s) 652, 851
HpyCH4III ACNGT 4 cut(s) 88, 362, 414, 750
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 389, 505, 722
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 668, 803, 872
HpyF3I CTNAG 4 cut(s) 98, 290, 477, 826
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 9 cut(s) 470, 532, 553, 583, 589, 658, 675, 685, 722
HspAI GCGC 1 cut(s) 22
KspI CCGCGG 1 cut(s) 620
Kzo9I GATC 3 cut(s) 392, 487, 790
LmnI GCTCC 2 cut(s) 670, 809
Lsp1109I GCAGC 3 cut(s) 334, 586, 674
LweI GCATC 1 cut(s) 97
MaeI CTAG 3 cut(s) 12, 443, 536
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 2 cut(s) 256, 778
MalI GATC 3 cut(s) 394, 489, 792
MboI GATC 3 cut(s) 392, 487, 790
MboII GAAGA 6 cut(s) 54, 57, 60, 63, 493, 829
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 2 cut(s) 507, 819
MluCI AATT 2 cut(s) 354, 711
MlyI GAGTC 2 cut(s) 455, 638
MmeI TCCRAC 1 cut(s) 732
MnlI CCTC 7 cut(s) 61, 64, 224, 418, 605, 783, 835
MseI TTAA 2 cut(s) 30, 174
MslI CAYNNNNRTG 1 cut(s) 584
MspA1I CMGCKG 1 cut(s) 619
MspI CCGG 3 cut(s) 111, 189, 807
MspR9I CCNGG 1 cut(s) 112
MvnI CGCG 1 cut(s) 619
MwoI GCNNNNNNNGC 4 cut(s) 185, 668, 803, 872
NciI CCSGG 1 cut(s) 112
NdeI CATATG 1 cut(s) 498
NdeII GATC 3 cut(s) 392, 487, 790
NlaIII CATG 9 cut(s) 470, 532, 553, 583, 589, 658, 675, 685, 722
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 778
NsbI TGCGCA 1 cut(s) 23
NspI RCATGY 2 cut(s) 532, 722
PaeI GCATGC 1 cut(s) 722
PciI ACATGT 1 cut(s) 528
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 3 cut(s) 324, 601, 664
PleI GAGTC 2 cut(s) 454, 637
PpsI GAGTC 2 cut(s) 454, 637
PscI ACATGT 1 cut(s) 528
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 2 cut(s) 328, 614
PsrI GAACNNNNNNTAC 2 cut(s) 566, 598
PsuI RGATCY 1 cut(s) 392
RsaI GTAC 2 cut(s) 142, 411
RsaNI GTAC 2 cut(s) 141, 410
RseI CAYNNNNRTG 1 cut(s) 584
SacII CCGCGG 1 cut(s) 620
SaqAI TTAA 2 cut(s) 30, 174
SatI GCNGC 3 cut(s) 323, 600, 663
Sau3AI GATC 3 cut(s) 392, 487, 790
Sau96I GGNCC 2 cut(s) 328, 614
ScaI AGTACT 1 cut(s) 411
SchI GAGTC 2 cut(s) 455, 638
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 2 cut(s) 507, 819
SetI ASST 8 cut(s) 29, 95, 330, 510, 667, 775, 814, 832
SfaNI GCATC 1 cut(s) 97
Sfr303I CCGCGG 1 cut(s) 620
SgrBI CCGCGG 1 cut(s) 620
SinI GGWCC 2 cut(s) 328, 614
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SphI GCATGC 1 cut(s) 722
Sse9I AATT 2 cut(s) 354, 711
SsiI CCGC 3 cut(s) 167, 617, 619
SspI AATATT 1 cut(s) 18
SspMI CTAG 3 cut(s) 12, 443, 536
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 4 cut(s) 88, 362, 414, 750
TaiI ACGT 1 cut(s) 29
TaqI TCGA 4 cut(s) 212, 229, 423, 789
TasI AATT 2 cut(s) 354, 711
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 30, 174
Tru9I TTAA 2 cut(s) 30, 174
TscAI CASTG 4 cut(s) 189, 631, 729, 877
TseFI GTSAC 1 cut(s) 778
TseI GCWGC 3 cut(s) 322, 599, 662
Tsp45I GTSAC 1 cut(s) 778
TspDTI ATGAA 6 cut(s) 35, 328, 455, 574, 671, 698
TspRI CASTG 4 cut(s) 189, 631, 729, 877
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 328, 614
XbaI TCTAGA 1 cut(s) 442
XceI RCATGY 2 cut(s) 532, 722
XmiI GTMKAC 1 cut(s) 769
XspI CTAG 3 cut(s) 12, 443, 536
ZrmI AGTACT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.