FvH4_5g18900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007512
Physical Location & Seq
Reverse (-)
10860716 .. 10862584
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18900.t1

Sequence Viewer

Length: 579 bp
ATGGCTGCTGAACACTCGGAAAATCTCCACAAACTCCTCGAAAGTTTTCAAGATGATCTCCAGGACCTCAACCTGACCCTTCAAAGGAAGTTGATCAAGCAGTTGGAGGAGCTGGTTGGGCCTCAAGAAATTTCCATTCGTGAGGAAATTGCTGCTTTTGAGAGAGAAAAGGCTGAGGTCGAAGAGATAGCTACTAGAATTAGTGAATGGATGGAAAATGTGAATAAGCAGTTTAAGGAGCAGTTGAAGGAAGAAATAGCTACTTCTCAGCCTGAGCATTTGGCGGCCTTTGGAATGTACTTGGGACACGATTTTGAGGCTGAGCTTGATGAAGCTGTTAATGGATTAAAATCAACAACTGAACAACCTAGGGGAGATGGTTTTGGCAAGGATCTGATGAAGAAGATTTTCACGATGCAGCTTGAGATACATGCTGCAGCTCAGAGTAATCTGGATGAGCTTAAAAAGGTTCTTAAAAAACTAGAAAGAAGGAAGGAATGGGTGGAGAATACGGAGAAGCAGCTTCAAGAACTAAAAGGAATGATTGAATTGGTGCAGAATGCTCTCGTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

22.24

Weight (kDa)

4.95

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 284
AclWI GGATC 1 cut(s) 399
AcsI RAATTY 1 cut(s) 129
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 5 cut(s) 50, 83, 247, 527, 548
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 9 cut(s) 112, 191, 260, 325, 335, 421, 440, 460, 523
AluI AGCT 9 cut(s) 112, 191, 260, 325, 335, 421, 440, 460, 523
AlwI GGATC 1 cut(s) 399
AoxI GGCC 2 cut(s) 119, 285
ApeKI GCWGC 6 cut(s) 5, 152, 418, 434, 437, 520
ApoI RAATTY 1 cut(s) 129
Asp700I GAANNNNTTC 2 cut(s) 45, 407
AspA2I CCTAGG 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 64, 119
AvaII GGWCC 1 cut(s) 64
AvrII CCTAGG 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 5 cut(s) 139, 421, 430, 449, 532
BccI CCATC 2 cut(s) 205, 371
BciT130I CCWGG 1 cut(s) 62
BclI TGATCA 1 cut(s) 93
BfaI CTAG 3 cut(s) 195, 369, 482
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 7 cut(s) 6, 153, 285, 419, 435, 438, 521
BlnI CCTAGG 1 cut(s) 368
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 7 cut(s) 7, 154, 286, 420, 436, 439, 522
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 119
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 44
Bpu10I CCTNAGC 2 cut(s) 174, 273
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 2 cut(s) 108, 443
BsaBI GATNNNNATC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 349
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 2 cut(s) 216, 460
BseJI GATNNNNATC 1 cut(s) 349
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMII CTCAG 5 cut(s) 165, 264, 281, 312, 455
BseRI GAGGAG 2 cut(s) 26, 122
BseXI GCAGC 5 cut(s) 139, 421, 430, 449, 532
BsgI GTGCAG 1 cut(s) 575
BshFI GGCC 2 cut(s) 121, 287
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 318
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 2 cut(s) 121, 287
Bsp143I GATC 3 cut(s) 55, 93, 391
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 1 cut(s) 284
BspANI GGCC 2 cut(s) 121, 287
BspCNI CTCAG 5 cut(s) 166, 265, 280, 313, 454
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 3 cut(s) 55, 93, 391
BssT1I CCWWGG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 62
Bst6I CTCTTC 1 cut(s) 177
BstDEI CTNAG 5 cut(s) 174, 267, 273, 321, 441
BstF5I GGATG 2 cut(s) 216, 460
BstKTI GATC 3 cut(s) 58, 96, 394
BstMBI GATC 3 cut(s) 55, 93, 391
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 1 cut(s) 434
BstSCI CCNGG 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 5 cut(s) 139, 421, 430, 449, 532
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 2 cut(s) 121, 287
BtsCI GGATG 2 cut(s) 216, 460
Cfr13I GGNCC 2 cut(s) 64, 119
Csp6I GTAC 1 cut(s) 298
CviAII CATG 1 cut(s) 431
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 5 cut(s) 174, 267, 273, 321, 441
DpnI GATC 3 cut(s) 57, 95, 393
DpnII GATC 3 cut(s) 55, 93, 391
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 64
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 368
FaeI CATG 1 cut(s) 434
FaiI YATR 1 cut(s) 432
FaqI GGGAC 1 cut(s) 318
FatI CATG 1 cut(s) 430
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 7 cut(s) 6, 153, 285, 419, 435, 438, 521
FokI GGATG 2 cut(s) 223, 467
Fsp4HI GCNGC 7 cut(s) 6, 153, 285, 419, 435, 438, 521
FspBI CTAG 3 cut(s) 195, 369, 482
GluI GCNGC 7 cut(s) 6, 153, 285, 419, 435, 438, 521
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 2 cut(s) 121, 287
Hin1II CATG 1 cut(s) 434
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 3 cut(s) 19, 396, 444
Hpy188III TCNNGA 6 cut(s) 50, 125, 140, 412, 452, 527
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 4 cut(s) 89, 241, 483, 487
HpyCH4V TGCA 3 cut(s) 418, 437, 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 5 cut(s) 174, 267, 273, 321, 441
Hsp92II CATG 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 3 cut(s) 55, 93, 391
LmnI GCTCC 2 cut(s) 109, 238
LpnPI CCDG 6 cut(s) 47, 74, 86, 98, 285, 437
Lsp1109I GCAGC 5 cut(s) 139, 421, 430, 449, 532
LweI GCATC 1 cut(s) 405
MaeI CTAG 3 cut(s) 195, 369, 482
MalI GATC 3 cut(s) 57, 95, 393
MboI GATC 3 cut(s) 55, 93, 391
MboII GAAGA 4 cut(s) 194, 263, 412, 415
MflI RGATCY 1 cut(s) 391
MluCI AATT 4 cut(s) 129, 147, 198, 548
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 7 cut(s) 47, 77, 100, 132, 136, 169, 310
MroXI GAANNNNTTC 2 cut(s) 45, 407
MseI TTAA 5 cut(s) 234, 339, 347, 462, 474
MspR9I CCNGG 1 cut(s) 62
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 118
NdeII GATC 3 cut(s) 55, 93, 391
NlaIII CATG 1 cut(s) 434
NspI RCATGY 1 cut(s) 434
PctI GAATGC 1 cut(s) 565
PdmI GAANNNNTTC 2 cut(s) 45, 407
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 7 cut(s) 7, 154, 286, 420, 436, 439, 522
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspPI GGNCC 2 cut(s) 64, 119
PspPPI RGGWCCY 1 cut(s) 64
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SaqAI TTAA 5 cut(s) 234, 339, 347, 462, 474
SatI GCNGC 7 cut(s) 6, 153, 285, 419, 435, 438, 521
Sau3AI GATC 3 cut(s) 55, 93, 391
Sau96I GGNCC 2 cut(s) 64, 119
ScrFI CCNGG 1 cut(s) 62
SfaNI GCATC 1 cut(s) 405
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 123, 422
SmoI CTYRAG 2 cut(s) 123, 422
Sse9I AATT 4 cut(s) 129, 147, 198, 548
SsiI CCGC 1 cut(s) 284
SspMI CTAG 3 cut(s) 195, 369, 482
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 368
TaqI TCGA 2 cut(s) 39, 180
TasI AATT 4 cut(s) 129, 147, 198, 548
TatI WGTACW 1 cut(s) 297
TauI GCSGC 1 cut(s) 287
Tru1I TTAA 5 cut(s) 234, 339, 347, 462, 474
Tru9I TTAA 5 cut(s) 234, 339, 347, 462, 474
TseI GCWGC 6 cut(s) 5, 152, 418, 434, 437, 520
TspDTI ATGAA 2 cut(s) 345, 413
TspGWI ACGGA 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 129
XceI RCATGY 1 cut(s) 434
XmaJI CCTAGG 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 45, 407
XspI CTAG 3 cut(s) 195, 369, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.