FvH4_5g20050

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014746
Physical Location & Seq
Reverse (-)
11824607 .. 11826270
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20050.t1

Sequence Viewer

Length: 849 bp
ATGGAAACTTTTAACCCTACTGGTGTTAGTATGTTGCTCACAATTGACACTACGCGCAACAGAGTTCTATTCGCCGAAACTAGCAAGGAAGCTGTCGATTTTCTTTTCACTCTTCTATCTTTGCCTGTTGCCACAGTCATTAGCCTCCTCTCTGGGGATGGTATGGTTGGTTGCATCGGAAATCTGTATGAGAGCGTTCAAGATCTCGGAGACACACACTTGGAACCCAACTTTAACAAGGACATGCTGCTGAAACCCAAAGCAACAGTTGTTGGTGCCGATATCCTTGGCGTAAACAACAATGTCTGTAAGTCTTGCAGCTGCCATTTCCCTGGATCTGTTTGTCCATTATGCGAATGCGCCGTGTCTACGACAGCTGCATCTACTGGAGAAGCGCCCTGCAGCAATGGAGGGTACGTCAAAGGTGGTGTTGTATTCGTGATTATGGATAACTTGGTAGTGAAGCCTATGTCTACCGTATCAATTCTAGATGTGCTTAAAGAGCTGAATGTTATGGAGGTTGACGCCCTTGAACAGAAGTTGGTTAGTCTTGGCAAGGAAGAGGGGCTGAAGTTGGTGAAGGCATCTTTGGAGTCGAATGCAGTTCTTAGCAGTGCATTCCTTGGTAACGCAATTGGGACTCCGGGCCAGTCCCGCACACATACTGATGGTTTGGAAGCTGAAATCCAGGGTGAATATGTTGCTCTTTCAGAATTCATTGGGTTAAATAGGGTAGAACAAGCAGATGTGTTCGATGAGAATTGGAAAAATGAAGAGAAAAAATGGAACAAATATGGCAAGTATAAAGCACTGAAGAAACTAAGAAACACAAGAACAAGGAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

283

Amino Acids

30.49

Weight (kDa)

5.27

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 9 - 211 1.4e-34 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 368, 473
AccII CGCG 1 cut(s) 55
AciI CCGC 1 cut(s) 655
AclWI GGATC 1 cut(s) 343
AcsI RAATTY 1 cut(s) 713
AcuI CTGAAG 2 cut(s) 590, 833
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 2 cut(s) 200, 533
AjnI CCWGG 2 cut(s) 331, 687
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 5 cut(s) 92, 321, 377, 505, 680
AluI AGCT 5 cut(s) 92, 321, 377, 505, 680
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 1 cut(s) 343
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 5 cut(s) 247, 318, 321, 377, 402
ApoI RAATTY 1 cut(s) 713
AspLEI GCGC 3 cut(s) 57, 362, 397
AspS9I GGNCC 1 cut(s) 646
AsuC2I CCSGG 1 cut(s) 645
AsuHPI GGTGA 2 cut(s) 589, 704
BanI GGYRCC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 234, 308, 330, 364, 414
BccI CCATC 2 cut(s) 152, 662
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 2 cut(s) 333, 689
BcnI CCSGG 1 cut(s) 645
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 2 cut(s) 81, 488
BfmI CTRYAG 1 cut(s) 400
BfoI RGCGCY 1 cut(s) 398
BglII AGATCT 1 cut(s) 202
BisI GCNGC 5 cut(s) 248, 319, 322, 378, 403
BlsI GCNGC 5 cut(s) 249, 320, 323, 379, 404
Bme1390I CCNGG 3 cut(s) 333, 645, 689
BmgT120I GGNCC 1 cut(s) 646
BmiI GGNNCC 2 cut(s) 225, 277
BmrFI CCNGG 3 cut(s) 333, 645, 689
BmsI GCATC 3 cut(s) 183, 389, 593
BpmI CTGGAG 1 cut(s) 408
BpuMI CCSGG 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 4 cut(s) 286, 331, 622, 688
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 3 cut(s) 25, 391, 649
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 2 cut(s) 333, 689
BseDI CCNNGG 4 cut(s) 286, 331, 622, 688
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 412
BseNI ACTGG 3 cut(s) 25, 391, 649
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 5 cut(s) 234, 308, 330, 364, 414
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 1 cut(s) 648
BshNI GGYRCC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 644
BslFI GGGAC 2 cut(s) 637, 652
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 2 cut(s) 637, 652
BsmI GAATGC 3 cut(s) 362, 604, 617
BsnI GGCC 1 cut(s) 648
Bsp143I GATC 2 cut(s) 202, 335
BspACI CCGC 1 cut(s) 655
BspANI GGCC 1 cut(s) 648
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 225, 277
BspMAI CTGCAG 1 cut(s) 404
BspPI GGATC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 412
BsrI ACTGG 3 cut(s) 25, 391, 649
BssECI CCNNGG 4 cut(s) 286, 331, 622, 688
BssMI GATC 2 cut(s) 202, 335
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 286, 622
Bst2UI CCWGG 2 cut(s) 333, 689
Bst4CI ACNGT 3 cut(s) 136, 268, 478
Bst6I CTCTTC 3 cut(s) 117, 555, 768
BstACI GRCGYC 1 cut(s) 525
BstDEI CTNAG 2 cut(s) 608, 821
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 3 cut(s) 57, 362, 397
BstKTI GATC 2 cut(s) 205, 338
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 2 cut(s) 202, 335
BstMWI GCNNNNNNNGC 2 cut(s) 502, 654
BstNI CCWGG 2 cut(s) 333, 689
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 3 cut(s) 331, 643, 687
BstSFI CTRYAG 1 cut(s) 400
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 5 cut(s) 234, 308, 330, 364, 414
BstX2I RGATCY 2 cut(s) 202, 335
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 2 cut(s) 202, 335
BsuRI GGCC 1 cut(s) 648
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 619, 809
CfoI GCGC 3 cut(s) 57, 362, 397
Cfr13I GGNCC 1 cut(s) 646
CseI GACGC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 415
CviAII CATG 1 cut(s) 244
CviJI RGCY 9 cut(s) 92, 144, 321, 377, 466, 505, 568, 648, 680
CviKI_1 RGCY 9 cut(s) 92, 144, 321, 377, 466, 505, 568, 648, 680
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 608, 821
DpnI GATC 2 cut(s) 204, 337
DpnII GATC 2 cut(s) 202, 335
Eam1104I CTCTTC 3 cut(s) 117, 555, 768
EarI CTCTTC 3 cut(s) 117, 555, 768
Eco130I CCWWGG 2 cut(s) 286, 622
Eco32I GATATC 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 590, 833
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 331, 687
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 286, 622
ErhI CCWWGG 2 cut(s) 286, 622
FaeI CATG 1 cut(s) 247
FaqI GGGAC 2 cut(s) 637, 652
FatI CATG 1 cut(s) 243
FauI CCCGC 1 cut(s) 662
FblI GTMKAC 2 cut(s) 368, 473
Fnu4HI GCNGC 5 cut(s) 248, 319, 322, 378, 403
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 5 cut(s) 248, 319, 322, 378, 403
FspBI CTAG 2 cut(s) 81, 488
GlaI GCGC 3 cut(s) 56, 361, 396
GluI GCNGC 5 cut(s) 248, 319, 322, 378, 403
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 398
HaeIII GGCC 1 cut(s) 648
HapII CCGG 1 cut(s) 644
HgaI GACGC 1 cut(s) 533
HhaI GCGC 3 cut(s) 57, 362, 397
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 3 cut(s) 55, 360, 395
HinP1I GCGC 3 cut(s) 55, 360, 395
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 2 cut(s) 593, 640
HpaII CCGG 1 cut(s) 644
HphI GGTGA 2 cut(s) 589, 704
Hpy166II GTNNAC 4 cut(s) 295, 369, 474, 523
Hpy188I TCNGA 3 cut(s) 179, 209, 712
Hpy188III TCNNGA 3 cut(s) 200, 439, 488
Hpy8I GTNNAC 4 cut(s) 295, 369, 474, 523
HpyAV CCTTC 1 cut(s) 574
HpyCH4III ACNGT 3 cut(s) 136, 268, 478
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 174, 318, 380, 402, 602, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 654
HpyF3I CTNAG 2 cut(s) 608, 821
HpySE526I ACGT 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 3 cut(s) 55, 360, 395
Kzo9I GATC 2 cut(s) 202, 335
Lsp1109I GCAGC 5 cut(s) 234, 308, 330, 364, 414
LweI GCATC 3 cut(s) 183, 389, 593
MaeI CTAG 2 cut(s) 81, 488
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 1 cut(s) 626
MalI GATC 2 cut(s) 204, 337
MboI GATC 2 cut(s) 202, 335
MboII GAAGA 4 cut(s) 104, 572, 785, 826
MfeI CAATTG 2 cut(s) 42, 633
MflI RGATCY 2 cut(s) 202, 335
MluCI AATT 5 cut(s) 42, 483, 633, 713, 760
MlyI GAGTC 2 cut(s) 602, 634
MnlI CCTC 5 cut(s) 155, 158, 404, 511, 556
MseI TTAA 4 cut(s) 12, 234, 498, 725
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 321, 377
MspI CCGG 1 cut(s) 644
MspR9I CCNGG 3 cut(s) 333, 645, 689
MunI CAATTG 2 cut(s) 42, 633
Mva1269I GAATGC 3 cut(s) 362, 604, 617
MvaI CCWGG 2 cut(s) 333, 689
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 502, 654
NciI CCSGG 1 cut(s) 645
NdeII GATC 2 cut(s) 202, 335
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 2 cut(s) 225, 277
NspI RCATGY 1 cut(s) 247
PctI GAATGC 3 cut(s) 362, 604, 617
PkrI GCNGC 5 cut(s) 249, 320, 323, 379, 404
PleI GAGTC 2 cut(s) 601, 634
PpsI GAGTC 2 cut(s) 601, 634
Psp6I CCWGG 2 cut(s) 331, 687
PspGI CCWGG 2 cut(s) 331, 687
PspN4I GGNNCC 2 cut(s) 225, 277
PspPI GGNCC 1 cut(s) 646
PstI CTGCAG 1 cut(s) 404
PsuI RGATCY 2 cut(s) 202, 335
PvuII CAGCTG 2 cut(s) 321, 377
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 666
SaqAI TTAA 4 cut(s) 12, 234, 498, 725
SatI GCNGC 5 cut(s) 248, 319, 322, 378, 403
Sau3AI GATC 2 cut(s) 202, 335
Sau96I GGNCC 1 cut(s) 646
SchI GAGTC 2 cut(s) 602, 634
ScrFI CCNGG 3 cut(s) 333, 645, 689
SetI ASST 8 cut(s) 94, 323, 379, 420, 427, 507, 522, 682
SfaNI GCATC 3 cut(s) 183, 389, 593
SfcI CTRYAG 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 666
Sse9I AATT 5 cut(s) 42, 483, 633, 713, 760
SsiI CCGC 1 cut(s) 655
SspMI CTAG 2 cut(s) 81, 488
StyD4I CCNGG 3 cut(s) 331, 643, 687
StyI CCWWGG 2 cut(s) 286, 622
TaaI ACNGT 3 cut(s) 136, 268, 478
TaiI ACGT 1 cut(s) 420
TaqI TCGA 3 cut(s) 96, 596, 753
TasI AATT 5 cut(s) 42, 483, 633, 713, 760
Tru1I TTAA 4 cut(s) 12, 234, 498, 725
Tru9I TTAA 4 cut(s) 12, 234, 498, 725
TscAI CASTG 2 cut(s) 619, 816
TseI GCWGC 5 cut(s) 247, 318, 321, 377, 402
TspDTI ATGAA 2 cut(s) 706, 786
TspRI CASTG 2 cut(s) 619, 816
XapI RAATTY 1 cut(s) 713
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 247
XmiI GTMKAC 2 cut(s) 368, 473
XspI CTAG 2 cut(s) 81, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.