FvH4_7g21290

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014746
Physical Location & Seq
Forward (+)
17101633 .. 17103362
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21290.t1

Sequence Viewer

Length: 834 bp
ATGGAAACTTTTAACCCTACTGGTGTTAGTATGTTGCTCACAATTGACACTAAGCGCAACAGAGTTCTATTCGCCGAAACTAGCAAGGAAGCTGTCGATTTTCTTTTCTCTCTTCTATCTTTGCCTGTTGCCACTGTCATTAGCCTCCTCTCTGGGGATGGCATGGTTGGTTGCATCGGAAATCTGTATGAGAGCGTTGAAGATCTAGGAGACACACACTTGGAGCCCAACTTTAACAAGGATATGCTGCTGAAACCCAAAGCAACAGTTGTTGGTGCCGATATCCTTGGCGTAAACAACAATGTCTGTAAGTCTTGCAGCTGCCATTTCCCTGGATATGTTTGTCCATTATGTGAATGCGCCGTGTCTACGACAGCTGCATCTACTGGAGAAGCGCCCTGCAGCAATGGAGGGTACGTCAAAGGTGGTGTTGTATTTGTGATCATGGATAATTTGGTAGTGAAGCCTATGTCTACCGTATCAATTATAGATGTGCTTAAAGAGCTGAATGTTATGGAGGTTGATGCCCTTGAACAGAAGTTGGTTAGTCTTGGCAAGGAAGAGGGGTTGAAGTTGGTGCAGGCATCTTTGGAGTCGAATACAGTTCTTAGCAGTATATTCCTTGGGTTCAGGAAGTCTCATATTGGTCCGATGGAGTGGCAAGGGCAAAAGCTGATGCTGATAATGCTGGCGCTGATACTGGTGATACTGCTGATGCCATTTGGGATGATGGAGTTTGGCACTGATTACATATCGATGAAGATGATGTTGCTGATGTTTGGTGGTAGTGTAGCTCTTACAGTAATGACCGCCCTAATTGATCACGAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

278

Amino Acids

29.88

Weight (kDa)

4.79

Isoelectric Point (pI)

24.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 9 - 212 5.1e-36 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 368, 473
AciI CCGC 1 cut(s) 810
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 3 cut(s) 200, 533, 571
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 6 cut(s) 92, 321, 377, 505, 673, 794
AluI AGCT 6 cut(s) 92, 321, 377, 505, 673, 794
Alw26I GTCTC 2 cut(s) 204, 642
ApeKI GCWGC 5 cut(s) 247, 318, 321, 377, 402
AspLEI GCGC 4 cut(s) 57, 362, 397, 694
AspS9I GGNCC 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 715
AvaII GGWCC 1 cut(s) 647
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 234, 308, 330, 364, 414
BccI CCATC 3 cut(s) 152, 646, 724
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 1 cut(s) 333
BclI TGATCA 2 cut(s) 441, 820
BcoDI GTCTC 2 cut(s) 204, 642
BfaI CTAG 2 cut(s) 81, 206
BfmI CTRYAG 1 cut(s) 400
BfoI RGCGCY 2 cut(s) 398, 695
BglII AGATCT 1 cut(s) 202
BisI GCNGC 5 cut(s) 248, 319, 322, 378, 403
BlsI GCNGC 5 cut(s) 249, 320, 323, 379, 404
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 2 cut(s) 225, 277
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 6 cut(s) 183, 389, 514, 593, 666, 705
BpmI CTGGAG 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 755
BsaJI CCNNGG 3 cut(s) 286, 331, 622
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 3 cut(s) 25, 391, 705
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 333
BseCI ATCGAT 1 cut(s) 755
BseDI CCNNGG 3 cut(s) 286, 331, 622
BseGI GGATG 2 cut(s) 163, 732
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 412
BseNI ACTGG 3 cut(s) 25, 391, 705
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 5 cut(s) 234, 308, 330, 364, 414
BsgI GTGCAG 1 cut(s) 599
BshNI GGYRCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 755
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 204, 642
BsmI GAATGC 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 202, 441, 820
BspACI CCGC 1 cut(s) 810
BspDI ATCGAT 1 cut(s) 755
BspLI GGNNCC 2 cut(s) 225, 277
BspMAI CTGCAG 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 412
BsrI ACTGG 3 cut(s) 25, 391, 705
BssECI CCNNGG 3 cut(s) 286, 331, 622
BssMI GATC 3 cut(s) 202, 441, 820
BssT1I CCWWGG 2 cut(s) 286, 622
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 5 cut(s) 136, 268, 478, 604, 802
Bst6I CTCTTC 2 cut(s) 117, 555
BstC8I GCNNGC 2 cut(s) 582, 690
BstDEI CTNAG 2 cut(s) 51, 608
BstF5I GGATG 2 cut(s) 163, 732
BstH2I RGCGCY 2 cut(s) 398, 695
BstHHI GCGC 4 cut(s) 57, 362, 397, 694
BstKTI GATC 3 cut(s) 205, 444, 823
BstMAI GTCTC 2 cut(s) 204, 642
BstMBI GATC 3 cut(s) 202, 441, 820
BstMWI GCNNNNNNNGC 2 cut(s) 502, 685
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 400
BstV1I GCAGC 5 cut(s) 234, 308, 330, 364, 414
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 755
BsuTUI ATCGAT 1 cut(s) 755
BtsCI GGATG 2 cut(s) 163, 732
BtsIMutI CAGTG 2 cut(s) 132, 741
Cac8I GCNNGC 2 cut(s) 582, 690
CfoI GCGC 4 cut(s) 57, 362, 397, 694
Cfr13I GGNCC 1 cut(s) 647
ClaI ATCGAT 1 cut(s) 755
Csp6I GTAC 1 cut(s) 415
CviAII CATG 2 cut(s) 163, 445
CviJI RGCY 9 cut(s) 92, 144, 226, 321, 377, 466, 505, 673, 794
CviKI_1 RGCY 9 cut(s) 92, 144, 226, 321, 377, 466, 505, 673, 794
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 51, 608
DpnI GATC 3 cut(s) 204, 443, 822
DpnII GATC 3 cut(s) 202, 441, 820
Eam1104I CTCTTC 2 cut(s) 117, 555
EarI CTCTTC 2 cut(s) 117, 555
Eco130I CCWWGG 2 cut(s) 286, 622
Eco24I GRGCYC 1 cut(s) 228
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 331
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 286, 622
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 2 cut(s) 286, 622
FaeI CATG 2 cut(s) 166, 448
FatI CATG 2 cut(s) 162, 444
FbaI TGATCA 2 cut(s) 441, 820
FblI GTMKAC 2 cut(s) 368, 473
Fnu4HI GCNGC 5 cut(s) 248, 319, 322, 378, 403
FokI GGATG 2 cut(s) 170, 739
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 5 cut(s) 248, 319, 322, 378, 403
FspBI CTAG 2 cut(s) 81, 206
GlaI GCGC 4 cut(s) 56, 361, 396, 693
GluI GCNGC 5 cut(s) 248, 319, 322, 378, 403
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 2 cut(s) 398, 695
HhaI GCGC 4 cut(s) 57, 362, 397, 694
Hin1II CATG 2 cut(s) 166, 448
Hin6I GCGC 4 cut(s) 55, 360, 395, 692
HinP1I GCGC 4 cut(s) 55, 360, 395, 692
HinfI GANTC 1 cut(s) 593
HphI GGTGA 1 cut(s) 715
Hpy166II GTNNAC 3 cut(s) 295, 369, 474
Hpy188I TCNGA 2 cut(s) 179, 651
Hpy188III TCNNGA 2 cut(s) 631, 824
Hpy8I GTNNAC 3 cut(s) 295, 369, 474
HpyCH4III ACNGT 5 cut(s) 136, 268, 478, 604, 802
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 174, 318, 380, 402, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 685
HpyF3I CTNAG 2 cut(s) 51, 608
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 2 cut(s) 166, 448
HspAI GCGC 4 cut(s) 55, 360, 395, 692
Ksp22I TGATCA 2 cut(s) 441, 820
Kzo9I GATC 3 cut(s) 202, 441, 820
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 5 cut(s) 234, 308, 330, 364, 414
LweI GCATC 6 cut(s) 183, 389, 514, 593, 666, 705
MaeI CTAG 2 cut(s) 81, 206
MaeII ACGT 1 cut(s) 417
MalI GATC 3 cut(s) 204, 443, 822
MboI GATC 3 cut(s) 202, 441, 820
MboII GAAGA 4 cut(s) 104, 212, 572, 772
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 4 cut(s) 42, 451, 483, 816
MlyI GAGTC 1 cut(s) 602
MnlI CCTC 5 cut(s) 155, 158, 404, 511, 556
MseI TTAA 3 cut(s) 12, 234, 498
MslI CAYNNNNRTG 1 cut(s) 755
MspA1I CMGCKG 2 cut(s) 321, 377
MspR9I CCNGG 1 cut(s) 333
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 502, 685
NdeII GATC 3 cut(s) 202, 441, 820
NlaIII CATG 2 cut(s) 166, 448
NlaIV GGNNCC 2 cut(s) 225, 277
PctI GAATGC 1 cut(s) 362
PkrI GCNGC 5 cut(s) 249, 320, 323, 379, 404
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 225, 277
PspPI GGNCC 1 cut(s) 647
PstI CTGCAG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 202
PvuII CAGCTG 2 cut(s) 321, 377
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 755
SaqAI TTAA 3 cut(s) 12, 234, 498
SatI GCNGC 5 cut(s) 248, 319, 322, 378, 403
Sau3AI GATC 3 cut(s) 202, 441, 820
Sau96I GGNCC 1 cut(s) 647
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 228
SetI ASST 9 cut(s) 94, 323, 379, 420, 427, 507, 522, 675, 796
SfaNI GCATC 6 cut(s) 183, 389, 514, 593, 666, 705
SfcI CTRYAG 1 cut(s) 400
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 755
Sse9I AATT 4 cut(s) 42, 451, 483, 816
SsiI CCGC 1 cut(s) 810
SspMI CTAG 2 cut(s) 81, 206
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 2 cut(s) 286, 622
TaaI ACNGT 5 cut(s) 136, 268, 478, 604, 802
TaiI ACGT 1 cut(s) 420
TaqI TCGA 3 cut(s) 96, 596, 755
TasI AATT 4 cut(s) 42, 451, 483, 816
Tru1I TTAA 3 cut(s) 12, 234, 498
Tru9I TTAA 3 cut(s) 12, 234, 498
TscAI CASTG 2 cut(s) 139, 748
TseI GCWGC 5 cut(s) 247, 318, 321, 377, 402
TspDTI ATGAA 1 cut(s) 773
TspRI CASTG 2 cut(s) 139, 748
VpaK11BI GGWCC 1 cut(s) 647
XmiI GTMKAC 2 cut(s) 368, 473
XspI CTAG 2 cut(s) 81, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.