Rh1AG332100

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014746
Physical Location & Seq
Forward (+)
56733099 .. 56733479
381 bp
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UTR
Exon/CDS
Intron
Rh1AG332100.1

Sequence Viewer

Length: 381 bp
ATGGTTGGCTGCCTAGGAAATCTTTACGAGAGCATTGAAGATCTCGGTGACACACACATGAAACCCAATTTTAACAAAAACATCCTGCTGAAACCCAAGACAACAGTTGTCGGTGCTAATATCCTTAGTGTAAACAAAAATGTATGTACGTCATGCAGCTGCCATTTCCCTGGAGATGTTTGTCCACTGTGTGAATGTGCCGTGTCTCCAACTGCTGCATCTACTAGAGAAGCGCCCTACAGTAATGGAGGGTACGTCAAGGGTGGTGTTACATACGTGATTATGGATAACTTGGCAGTGAAGCCCATGTCGACCGTATCTATTGTTGATGTGCTAAAAGAGTTGAATGTTATGGAGGTTGGTGTGCTTGAACAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

126

Amino Acids

13.44

Weight (kDa)

5.59

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 78 - 118 3.5e-07 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 311
AdeI CACNNNGTG 1 cut(s) 191
AfaI GTAC 2 cut(s) 148, 254
AgsI TTSAA 3 cut(s) 38, 346, 371
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 1 cut(s) 159
AluI AGCT 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 210
ApeKI GCWGC 4 cut(s) 9, 156, 159, 215
AspA2I CCTAGG 1 cut(s) 13
AspLEI GCGC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 59
AvrII CCTAGG 1 cut(s) 13
BbvI GCAGC 3 cut(s) 146, 168, 202
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 2 cut(s) 14, 225
BfmI CTRYAG 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 236
BglII AGATCT 1 cut(s) 40
BisI GCNGC 4 cut(s) 10, 157, 160, 216
BlnI CCTAGG 1 cut(s) 13
BlsI GCNGC 4 cut(s) 11, 158, 161, 217
Bme1390I CCNGG 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 277
BsaJI CCNNGG 2 cut(s) 13, 169
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 13, 169
BseGI GGATG 1 cut(s) 81
BseXI GCAGC 3 cut(s) 146, 168, 202
Bsh1285I CGRYCG 1 cut(s) 315
BsiEI CGRYCG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 210
Bsp143I GATC 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 13, 169
BssMI GATC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 4 cut(s) 106, 189, 242, 316
BstBAI YACGTR 1 cut(s) 277
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 1 cut(s) 40
BstMCI CGRYCG 1 cut(s) 315
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 238
BstV1I GCAGC 3 cut(s) 146, 168, 202
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 1 cut(s) 170
BstYI RGATCY 1 cut(s) 40
BtsCI GGATG 1 cut(s) 81
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 2 cut(s) 185, 303
CfoI GCGC 1 cut(s) 235
Csp6I GTAC 2 cut(s) 147, 253
CviAII CATG 3 cut(s) 58, 153, 307
CviJI RGCY 3 cut(s) 9, 159, 304
CviKI_1 RGCY 3 cut(s) 9, 159, 304
CviQI GTAC 2 cut(s) 147, 253
DdeI CTNAG 1 cut(s) 125
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DraIII CACNNNGTG 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 13
FaeI CATG 3 cut(s) 61, 156, 310
FaiI YATR 7 cut(s) 59, 145, 154, 274, 284, 308, 353
FatI CATG 3 cut(s) 57, 152, 306
FblI GTMKAC 1 cut(s) 311
Fnu4HI GCNGC 4 cut(s) 10, 157, 160, 216
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 4 cut(s) 10, 157, 160, 216
FspBI CTAG 2 cut(s) 14, 225
GlaI GCGC 1 cut(s) 234
GluI GCNGC 4 cut(s) 10, 157, 160, 216
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 236
HhaI GCGC 1 cut(s) 235
Hin1II CATG 3 cut(s) 61, 156, 310
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 3 cut(s) 133, 185, 312
Hpy8I GTNNAC 3 cut(s) 133, 185, 312
HpyCH4III ACNGT 4 cut(s) 106, 189, 242, 316
HpyCH4IV ACGT 3 cut(s) 149, 255, 276
HpyCH4V TGCA 2 cut(s) 156, 218
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 3 cut(s) 149, 255, 276
Hsp92II CATG 3 cut(s) 61, 156, 310
HspAI GCGC 1 cut(s) 233
Kzo9I GATC 1 cut(s) 40
LpnPI CCDG 3 cut(s) 98, 156, 183
Lsp1109I GCAGC 3 cut(s) 146, 168, 202
LweI GCATC 1 cut(s) 227
MaeI CTAG 2 cut(s) 14, 225
MaeII ACGT 3 cut(s) 149, 255, 276
MaeIII GTNAC 2 cut(s) 47, 268
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 50
MflI RGATCY 1 cut(s) 40
MluCI AATT 1 cut(s) 67
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 2 cut(s) 242, 349
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
NdeII GATC 1 cut(s) 40
NlaIII CATG 3 cut(s) 61, 156, 310
NmuCI GTSAC 1 cut(s) 47
PkrI GCNGC 4 cut(s) 11, 158, 161, 217
Ppu21I YACGTR 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 159
RsaI GTAC 2 cut(s) 148, 254
RsaNI GTAC 2 cut(s) 147, 253
RseI CAYNNNNRTG 1 cut(s) 56
SalI GTCGAC 1 cut(s) 310
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 4 cut(s) 10, 157, 160, 216
Sau3AI GATC 1 cut(s) 40
ScrFI CCNGG 1 cut(s) 171
SetI ASST 5 cut(s) 152, 161, 258, 279, 360
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 1 cut(s) 67
SspMI CTAG 2 cut(s) 14, 225
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 13
TaaI ACNGT 4 cut(s) 106, 189, 242, 316
TaiI ACGT 3 cut(s) 152, 258, 279
TaqI TCGA 1 cut(s) 311
TasI AATT 1 cut(s) 67
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 2 cut(s) 192, 303
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 4 cut(s) 9, 156, 159, 215
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 74
TspRI CASTG 2 cut(s) 192, 303
XmaJI CCTAGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 311
XspI CTAG 2 cut(s) 14, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.