Rmu_sc0005964.1_g000002

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005964.1
OG0014746
Physical Location & Seq
Forward (+)
4555 .. 6143
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005964.1_g000002.1.cds

Sequence Viewer

Length: 414 bp
atggagggtatcaagggtggtgttacatacgtgattatggataacttggtagtgaagcctatatctaccatatcaattgtagatgtgcttaaagagttgaatgttatggaggttggtgcccttgaacataagttggttagtcttgacacagaagaggggctaaagttggtgaaggcatctttggagtcaaatacagttctcagcatgtatgccttgagctccaagagctcattattgcgtgaaacagtaagacccgacctgattacccaaccttactttatgatttcgtctccgataacaagcatagcatccactaaagctctgaaattgaacgattcagcctacccagctagcagcgtcactgatttgtcgctcgtcaacgccgccgtaaaggacatggagcttcaggtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

137

Amino Acids

14.77

Weight (kDa)

4.93

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 389
AgsI TTSAA 3 cut(s) 100, 125, 331
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 5 cut(s) 219, 228, 320, 350, 403
AluI AGCT 5 cut(s) 219, 228, 320, 350, 403
Alw21I GWGCWC 2 cut(s) 221, 230
Alw26I GTCTC 1 cut(s) 294
ApeKI GCWGC 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 181
AsuNHI GCTAGC 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 221, 230
Bbv12I GWGCWC 2 cut(s) 221, 230
BbvI GCAGC 1 cut(s) 366
BceAI ACGGC 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 2 cut(s) 351, 412
BisI GCNGC 2 cut(s) 355, 384
BlsI GCNGC 2 cut(s) 356, 385
BmiI GGNNCC 1 cut(s) 118
BmsI GCATC 2 cut(s) 185, 317
BmtI GCTAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 31
BseGI GGATG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 214
BseSI GKGCMC 1 cut(s) 121
BseXI GCAGC 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 346
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 2 cut(s) 221, 230
BsmAI GTCTC 1 cut(s) 294
BsmBI CGTCTC 1 cut(s) 294
Bsp1286I GDGCHC 3 cut(s) 121, 221, 230
BspACI CCGC 1 cut(s) 384
BspCNI CTCAG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 118
BspOI GCTAGC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 196, 247
Bst6I CTCTTC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 308
BstMAI GTCTC 1 cut(s) 294
BstMWI GCNNNNNNNGC 2 cut(s) 225, 347
BstNSI RCATGY 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 121
BstV1I GCAGC 1 cut(s) 366
BtsCI GGATG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 352
CseI GACGC 1 cut(s) 346
CviAII CATG 2 cut(s) 205, 397
CviJI RGCY 8 cut(s) 58, 160, 219, 228, 320, 341, 350, 403
CviKI_1 RGCY 8 cut(s) 58, 160, 219, 228, 320, 341, 350, 403
DdeI CTNAG 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Ecl136II GAGCTC 2 cut(s) 219, 228
Eco24I GRGCYC 2 cut(s) 221, 230
Eco53kI GAGCTC 2 cut(s) 219, 228
Eco57I CTGAAG 1 cut(s) 389
EcoICRI GAGCTC 2 cut(s) 219, 228
EcoT38I GRGCYC 2 cut(s) 221, 230
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 2 cut(s) 208, 400
FatI CATG 2 cut(s) 204, 396
Fnu4HI GCNGC 2 cut(s) 355, 384
FokI GGATG 1 cut(s) 295
FriOI GRGCYC 2 cut(s) 221, 230
Fsp4HI GCNGC 2 cut(s) 355, 384
FspBI CTAG 2 cut(s) 351, 412
GluI GCNGC 2 cut(s) 355, 384
GsaI CCCAGC 1 cut(s) 350
HgaI GACGC 1 cut(s) 346
Hin1II CATG 2 cut(s) 208, 400
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 2 cut(s) 185, 335
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 2 cut(s) 294, 324
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 2 cut(s) 196, 247
HpyCH4IV ACGT 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 347
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 2 cut(s) 208, 400
LmnI GCTCC 2 cut(s) 224, 400
LpnPI CCDG 3 cut(s) 272, 360, 392
Lsp1109I GCAGC 1 cut(s) 366
LweI GCATC 2 cut(s) 185, 317
MaeI CTAG 2 cut(s) 351, 412
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 2 cut(s) 22, 358
MboII GAAGA 1 cut(s) 164
MfeI CAATTG 1 cut(s) 75
MhlI GDGCHC 3 cut(s) 121, 221, 230
MluCI AATT 2 cut(s) 75, 326
MlyI GAGTC 1 cut(s) 194
MnlI CCTC 2 cut(s) 103, 148
MseI TTAA 1 cut(s) 90
MunI CAATTG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 225, 347
NheI GCTAGC 1 cut(s) 350
NlaIII CATG 2 cut(s) 208, 400
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 358
NspI RCATGY 1 cut(s) 208
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 2 cut(s) 356, 385
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Ppu21I YACGTR 1 cut(s) 31
Psp124BI GAGCTC 2 cut(s) 221, 230
PspFI CCCAGC 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 118
SacI GAGCTC 2 cut(s) 221, 230
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 2 cut(s) 355, 384
SchI GAGTC 1 cut(s) 194
SduI GDGCHC 3 cut(s) 121, 221, 230
SfaNI GCATC 2 cut(s) 185, 317
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 2 cut(s) 75, 326
SsiI CCGC 1 cut(s) 384
SspMI CTAG 2 cut(s) 351, 412
SstI GAGCTC 2 cut(s) 221, 230
TaaI ACNGT 2 cut(s) 196, 247
TaiI ACGT 1 cut(s) 33
TasI AATT 2 cut(s) 75, 326
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 354
Tsp45I GTSAC 1 cut(s) 358
TspRI CASTG 1 cut(s) 367
XceI RCATGY 1 cut(s) 208
XspI CTAG 2 cut(s) 351, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.