Rh5BG177300

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014746
Physical Location & Seq
Reverse (-)
19168479 .. 19170722
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG177300.1

Sequence Viewer

Length: 309 bp
ATGGGTAACTTGGTAGTGAAGCCTATGTCTACCATATCAATTGTAGATGTGCTTAAAGAGTTGAATGTTATGGAGGTTGGTGCCCTTGAACAGAAGTTGGTTAGTCTTGGCACGAAAGAGGGGCTAAAGTTGGTGAAGGCATCTTTGGAGTCAAATACAGTTCTTAGCAATATATTCCTTGGCTTTGTTGAGATTTGGCACTTCAATCAGATCTTTGCCAATGAGTCACTTGAATATTTGCCAATGAGTCACTTGAATATTACAAAAATTGTGCAGGCATGCTGTAAAGGTACAGGATATGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

102

Amino Acids

11.26

Weight (kDa)

5.6

Isoelectric Point (pI)

23.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 3 - 63 3.4e-07 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 29
AfaI GTAC 1 cut(s) 292
AgsI TTSAA 5 cut(s) 64, 89, 205, 233, 256
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AsuHPI GGTGA 1 cut(s) 145
BaeGI GKGCMC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 80
BglII AGATCT 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 82
BmsI GCATC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 178
BseDI CCNNGG 1 cut(s) 178
BseSI GKGCMC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 178
Bst4CI ACNGT 1 cut(s) 160
BstC8I GCNNGC 2 cut(s) 276, 280
BstDEI CTNAG 1 cut(s) 164
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstNSI RCATGY 1 cut(s) 282
BstSLI GKGCMC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 276, 280
Csp6I GTAC 1 cut(s) 291
CviAII CATG 1 cut(s) 279
CviJI RGCY 3 cut(s) 22, 124, 183
CviKI_1 RGCY 3 cut(s) 22, 124, 183
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 1 cut(s) 164
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 1 cut(s) 282
FaiI YATR 6 cut(s) 26, 35, 71, 173, 280, 300
FatI CATG 1 cut(s) 278
FblI GTMKAC 1 cut(s) 29
Hin1II CATG 1 cut(s) 282
HinfI GANTC 3 cut(s) 149, 224, 247
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 1 cut(s) 210
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 274
HpyF3I CTNAG 1 cut(s) 164
Hsp92II CATG 1 cut(s) 282
Kzo9I GATC 1 cut(s) 210
LpnPI CCDG 2 cut(s) 260, 279
LweI GCATC 1 cut(s) 149
MaeIII GTNAC 3 cut(s) 5, 225, 248
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 2 cut(s) 39, 267
MlyI GAGTC 3 cut(s) 158, 233, 256
MnlI CCTC 2 cut(s) 67, 112
MseI TTAA 2 cut(s) 54, 307
MunI CAATTG 1 cut(s) 39
NdeII GATC 1 cut(s) 210
NlaIII CATG 1 cut(s) 282
NlaIV GGNNCC 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 225, 248
NspI RCATGY 1 cut(s) 282
PaeI GCATGC 1 cut(s) 282
PleI GAGTC 3 cut(s) 157, 232, 255
PpsI GAGTC 3 cut(s) 157, 232, 255
PspN4I GGNNCC 1 cut(s) 82
PsuI RGATCY 1 cut(s) 210
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 54, 307
Sau3AI GATC 1 cut(s) 210
SchI GAGTC 3 cut(s) 158, 233, 256
SduI GDGCHC 1 cut(s) 85
SetI ASST 2 cut(s) 78, 292
SfaNI GCATC 1 cut(s) 149
SgeI CNNG 9 cut(s) 22, 98, 119, 124, 191, 242, 265, 287, 291
SphI GCATGC 1 cut(s) 282
Sse9I AATT 2 cut(s) 39, 267
SspI AATATT 2 cut(s) 236, 259
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 160
TasI AATT 2 cut(s) 39, 267
Tru1I TTAA 2 cut(s) 54, 307
Tru9I TTAA 2 cut(s) 54, 307
TseFI GTSAC 2 cut(s) 225, 248
Tsp45I GTSAC 2 cut(s) 225, 248
XceI RCATGY 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.