Rh4DG114800

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014746
Physical Location & Seq
Forward (+)
18112790 .. 18123898
11109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG114800.1

Sequence Viewer

Length: 372 bp
ATGAAGCATGGTTGTTCGTTGCTCTTCCAATTCAGATCGGTGACTACTGCGTCCGATTGGAAGTTCGTTGTTCTTTTAAGAGTTCTCTACTGGAGAAGCGTTGTGAGCAATGGAGGGTACGTCAAGGGTGGTGTTACATACTTGATTATGGATAACTTGGTAGTGAAGCCTATGTCTACCGTATCAATTGTAGATGTGCTTAAAGAGTTGAATGTTATGGAGGTTGGTGTCCTTGAACAGAAGTTGGTTGGTCTTGGCACGGAAGAGGGGCTAAAGTTGGTGAAGGCATCTTTGGAGTCAAATACAGTTCTCAGCAATATATTCCTTGGTAATGAACTGCATAAATTACGTTTCAACTCTGGTGCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

123

Amino Acids

13.66

Weight (kDa)

9.13

Isoelectric Point (pI)

20.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 36 - 112 2e-12 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 176
AfaI GTAC 1 cut(s) 119
AgsI TTSAA 3 cut(s) 211, 236, 355
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AsuHPI GGTGA 2 cut(s) 52, 292
BmsI GCATC 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 325
BseNI ACTGG 1 cut(s) 95
Bsp143I GATC 1 cut(s) 35
BspCNI CTCAG 1 cut(s) 324
BspQI GCTCTTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 115
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 325
Bst4CI ACNGT 2 cut(s) 181, 307
Bst6I CTCTTC 2 cut(s) 29, 258
BstDEI CTNAG 1 cut(s) 311
BstKTI GATC 1 cut(s) 38
BstMBI GATC 1 cut(s) 35
BstMWI GCNNNNNNNGC 1 cut(s) 105
CseI GACGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 8
CviJI RGCY 2 cut(s) 169, 271
CviKI_1 RGCY 2 cut(s) 169, 271
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 311
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
DrdI GACNNNNNNGTC 1 cut(s) 49
DseDI GACNNNNNNGTC 1 cut(s) 49
Eam1104I CTCTTC 2 cut(s) 29, 258
EarI CTCTTC 2 cut(s) 29, 258
Eco130I CCWWGG 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 1 cut(s) 11
FaiI YATR 7 cut(s) 9, 139, 149, 173, 218, 320, 342
FatI CATG 1 cut(s) 7
FblI GTMKAC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 112
HgaI GACGC 1 cut(s) 39
Hin1II CATG 1 cut(s) 11
HinfI GANTC 1 cut(s) 296
HphI GGTGA 2 cut(s) 52, 292
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 2 cut(s) 35, 55
Hpy8I GTNNAC 1 cut(s) 177
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 2 cut(s) 181, 307
HpyCH4IV ACGT 2 cut(s) 120, 349
HpyCH4V TGCA 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 2 cut(s) 120, 349
Hsp92II CATG 1 cut(s) 11
Kzo9I GATC 1 cut(s) 35
LguI GCTCTTC 1 cut(s) 29
LpnPI CCDG 2 cut(s) 76, 345
LweI GCATC 1 cut(s) 296
MaeII ACGT 2 cut(s) 120, 349
MaeIII GTNAC 2 cut(s) 40, 133
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MboII GAAGA 2 cut(s) 16, 275
MfeI CAATTG 1 cut(s) 186
MluCI AATT 3 cut(s) 29, 186, 344
MlyI GAGTC 1 cut(s) 305
MnlI CCTC 3 cut(s) 107, 214, 259
MseI TTAA 2 cut(s) 77, 201
MunI CAATTG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 105
NdeII GATC 1 cut(s) 35
NlaIII CATG 1 cut(s) 11
NmuCI GTSAC 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 29
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 77, 201
Sau3AI GATC 1 cut(s) 35
SchI GAGTC 1 cut(s) 305
SetI ASST 3 cut(s) 123, 225, 352
SfaNI GCATC 1 cut(s) 296
SgeI CNNG 9 cut(s) 20, 103, 136, 154, 169, 245, 266, 271, 338
Sse9I AATT 3 cut(s) 29, 186, 344
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 2 cut(s) 181, 307
TaiI ACGT 2 cut(s) 123, 352
TasI AATT 3 cut(s) 29, 186, 344
Tru1I TTAA 2 cut(s) 77, 201
Tru9I TTAA 2 cut(s) 77, 201
TseFI GTSAC 1 cut(s) 40
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 17, 348
TspGWI ACGGA 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.