RLG00000027410

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014746
Physical Location & Seq
Reverse (-)
11315255 .. 11316357
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027410

Sequence Viewer

Length: 666 bp
ATGATGGTATGGTTGGTTGCCTTAGATCTTTATGAGACCGTTGAAGATCTCGGTGACACACACATGGACCCAACTTTAACAAGAACATCCTGCTCAAACCCAAGACAAATTGTTGGTGCTAATATCCTTGGTGTAAACAAAAATGTCTGTACGTCTTGTAGCTGCCATTTCCCTGGAGATGTTTGTCCACTGTGTGAATGCGCTTTGTCTCCAACAGCTGCATCTACTGGAGAAGCGCCCTGCAGTAATGGAGGTTACGTCAAGGGTGGTGTTACATACGTGATTATGGATAACTTGGTAGTGAAGCCCATGTCTACCGTATCCATTGTAGATGTGCTAAAAGAGTTAAATGTTATGGAGGGGCTGAAGTTGGTGAAAGCATCTTTGGAGTCAAATACAGTTCTCAGCAATGTATTCCTTGGTAATGAACTGCATAAACGACGTTTCAACTTTGGTGCTCTAGATATGCAATTAGCCTCTTGGGTCCAGGTAGTCTCAGATCGGTCAGATGGAGTGGCAAGGGCAAAAGCTGGCAAAGCAGCTGATGCTGATACTGCTAATGTCATTTGCGATTGGGGGGCTTCTCACTGGTTACATATCGATGAAGATGATGTTGCTGATGTTTGCTGGTGGTGTGGTTCTGACTGCAATGACCGCATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

222

Amino Acids

23.83

Weight (kDa)

4.66

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 79 - 118 7e-07 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 314
AciI CCGC 1 cut(s) 655
AcuI CTGAAG 1 cut(s) 386
AdeI CACNNNGTG 1 cut(s) 194
AfaI GTAC 1 cut(s) 151
AgsI TTSAA 2 cut(s) 44, 448
AjnI CCWGG 2 cut(s) 172, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 4 cut(s) 162, 218, 530, 542
AluI AGCT 4 cut(s) 162, 218, 530, 542
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 3 cut(s) 29, 213, 499
ApeKI GCWGC 3 cut(s) 162, 218, 539
AseI ATTAAT 1 cut(s) 660
AspLEI GCGC 2 cut(s) 203, 238
AspS9I GGNCC 2 cut(s) 67, 484
AsuHPI GGTGA 2 cut(s) 65, 385
AvaII GGWCC 2 cut(s) 67, 484
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 3 cut(s) 149, 205, 551
BccI CCATC 1 cut(s) 503
BciT130I CCWGG 2 cut(s) 174, 488
BciVI GTATCC 1 cut(s) 331
BcoDI GTCTC 3 cut(s) 29, 213, 499
BfaI CTAG 1 cut(s) 461
BfmI CTRYAG 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 239
BfuI GTATCC 1 cut(s) 331
BglII AGATCT 2 cut(s) 25, 46
BisI GCNGC 3 cut(s) 163, 219, 540
BlsI GCNGC 3 cut(s) 164, 220, 541
Bme1390I CCNGG 2 cut(s) 174, 488
Bme18I GGWCC 2 cut(s) 67, 484
BmgT120I GGNCC 2 cut(s) 67, 484
BmiI GGNNCC 2 cut(s) 69, 485
BmrFI CCNGG 2 cut(s) 174, 488
BmsI GCATC 3 cut(s) 230, 389, 535
BpmI CTGGAG 2 cut(s) 195, 249
Bsa29I ATCGAT 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 127, 172, 418
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bse1I ACTGG 2 cut(s) 232, 593
Bse3DI GCAATG 2 cut(s) 415, 655
BseBI CCWGG 2 cut(s) 174, 488
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 127, 172, 418
BseGI GGATG 1 cut(s) 86
BseMI GCAATG 2 cut(s) 415, 655
BseMII CTCAG 2 cut(s) 418, 510
BseNI ACTGG 2 cut(s) 232, 593
BseXI GCAGC 3 cut(s) 149, 205, 551
BshVI ATCGAT 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 460
BsmAI GTCTC 3 cut(s) 29, 213, 499
BsmI GAATGC 1 cut(s) 203
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 3 cut(s) 25, 46, 499
BspACI CCGC 1 cut(s) 655
BspCNI CTCAG 2 cut(s) 417, 509
BspDI ATCGAT 1 cut(s) 600
BspLI GGNNCC 2 cut(s) 69, 485
BspMAI CTGCAG 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 29
BsrDI GCAATG 2 cut(s) 415, 655
BsrI ACTGG 2 cut(s) 232, 593
BssECI CCNNGG 3 cut(s) 127, 172, 418
BssMI GATC 3 cut(s) 25, 46, 499
BssT1I CCWWGG 2 cut(s) 127, 418
Bst2UI CCWGG 2 cut(s) 174, 488
Bst4CI ACNGT 4 cut(s) 40, 192, 319, 400
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 3 cut(s) 22, 404, 496
BstF5I GGATG 1 cut(s) 86
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 2 cut(s) 203, 238
BstKTI GATC 3 cut(s) 28, 49, 502
BstMAI GTCTC 3 cut(s) 29, 213, 499
BstMBI GATC 3 cut(s) 25, 46, 499
BstMWI GCNNNNNNNGC 4 cut(s) 536, 545, 554, 654
BstNI CCWGG 2 cut(s) 174, 488
BstSCI CCNGG 2 cut(s) 172, 486
BstSFI CTRYAG 1 cut(s) 241
BstV1I GCAGC 3 cut(s) 149, 205, 551
BstX2I RGATCY 2 cut(s) 25, 46
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 2 cut(s) 25, 46
Bsu15I ATCGAT 1 cut(s) 600
BsuI GTATCC 1 cut(s) 331
BsuTUI ATCGAT 1 cut(s) 600
BtsCI GGATG 1 cut(s) 86
BtsIMutI CAGTG 2 cut(s) 188, 586
Cac8I GCNNGC 1 cut(s) 532
CfoI GCGC 2 cut(s) 203, 238
Cfr13I GGNCC 2 cut(s) 67, 484
ClaI ATCGAT 1 cut(s) 600
Csp6I GTAC 1 cut(s) 150
CviAII CATG 2 cut(s) 64, 310
CviJI RGCY 8 cut(s) 162, 218, 307, 364, 476, 530, 542, 581
CviKI_1 RGCY 8 cut(s) 162, 218, 307, 364, 476, 530, 542, 581
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 3 cut(s) 22, 404, 496
DpnI GATC 3 cut(s) 27, 48, 501
DpnII GATC 3 cut(s) 25, 46, 499
DraIII CACNNNGTG 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 127, 418
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 2 cut(s) 67, 484
Eco57I CTGAAG 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 172, 486
EcoT14I CCWWGG 2 cut(s) 127, 418
ErhI CCWWGG 2 cut(s) 127, 418
FaeI CATG 2 cut(s) 67, 313
FatI CATG 2 cut(s) 63, 309
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 3 cut(s) 163, 219, 540
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 3 cut(s) 163, 219, 540
FspBI CTAG 1 cut(s) 461
GlaI GCGC 2 cut(s) 202, 237
GluI GCNGC 3 cut(s) 163, 219, 540
GsuI CTGGAG 2 cut(s) 195, 249
HaeII RGCGCY 1 cut(s) 239
HhaI GCGC 2 cut(s) 203, 238
Hin1II CATG 2 cut(s) 67, 313
Hin6I GCGC 2 cut(s) 201, 236
HinP1I GCGC 2 cut(s) 201, 236
HinfI GANTC 1 cut(s) 389
HphI GGTGA 2 cut(s) 65, 385
Hpy166II GTNNAC 3 cut(s) 136, 188, 315
Hpy188I TCNGA 3 cut(s) 499, 508, 643
Hpy188III TCNNGA 1 cut(s) 461
Hpy8I GTNNAC 3 cut(s) 136, 188, 315
Hpy99I CGWCG 1 cut(s) 444
HpyCH4III ACNGT 4 cut(s) 40, 192, 319, 400
HpyCH4IV ACGT 4 cut(s) 152, 258, 279, 442
HpyCH4V TGCA 5 cut(s) 221, 243, 433, 469, 648
HpyF10VI GCNNNNNNNGC 4 cut(s) 536, 545, 554, 654
HpyF3I CTNAG 3 cut(s) 22, 404, 496
HpySE526I ACGT 4 cut(s) 152, 258, 279, 442
Hsp92II CATG 2 cut(s) 67, 313
HspAI GCGC 2 cut(s) 201, 236
Kzo9I GATC 3 cut(s) 25, 46, 499
Lsp1109I GCAGC 3 cut(s) 149, 205, 551
LweI GCATC 3 cut(s) 230, 389, 535
MaeI CTAG 1 cut(s) 461
MaeII ACGT 4 cut(s) 152, 258, 279, 442
MaeIII GTNAC 4 cut(s) 53, 254, 271, 591
MalI GATC 3 cut(s) 27, 48, 501
MboI GATC 3 cut(s) 25, 46, 499
MboII GAAGA 2 cut(s) 56, 617
MflI RGATCY 2 cut(s) 25, 46
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 3 cut(s) 108, 470, 661
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 3 cut(s) 245, 352, 487
MseI TTAA 3 cut(s) 77, 347, 660
MslI CAYNNNNRTG 2 cut(s) 62, 600
MspA1I CMGCKG 2 cut(s) 218, 542
MspR9I CCNGG 2 cut(s) 174, 488
Mva1269I GAATGC 1 cut(s) 203
MvaI CCWGG 2 cut(s) 174, 488
MwoI GCNNNNNNNGC 4 cut(s) 536, 545, 554, 654
NdeII GATC 3 cut(s) 25, 46, 499
NlaIII CATG 2 cut(s) 67, 313
NlaIV GGNNCC 2 cut(s) 69, 485
NmuCI GTSAC 1 cut(s) 53
PctI GAATGC 1 cut(s) 203
PkrI GCNGC 3 cut(s) 164, 220, 541
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Ppu21I YACGTR 1 cut(s) 280
PshBI ATTAAT 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 172, 486
PspGI CCWGG 2 cut(s) 172, 486
PspN4I GGNNCC 2 cut(s) 69, 485
PspPI GGNCC 2 cut(s) 67, 484
PstI CTGCAG 1 cut(s) 245
PsuI RGATCY 2 cut(s) 25, 46
PvuII CAGCTG 2 cut(s) 218, 542
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
RseI CAYNNNNRTG 2 cut(s) 62, 600
SaqAI TTAA 3 cut(s) 77, 347, 660
SatI GCNGC 3 cut(s) 163, 219, 540
Sau3AI GATC 3 cut(s) 25, 46, 499
Sau96I GGNCC 2 cut(s) 67, 484
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 2 cut(s) 174, 488
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 3 cut(s) 230, 389, 535
SfcI CTRYAG 1 cut(s) 241
SinI GGWCC 2 cut(s) 67, 484
SmiMI CAYNNNNRTG 2 cut(s) 62, 600
Sse9I AATT 3 cut(s) 108, 470, 661
SsiI CCGC 1 cut(s) 655
SspMI CTAG 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 172, 486
StyI CCWWGG 2 cut(s) 127, 418
TaaI ACNGT 4 cut(s) 40, 192, 319, 400
TaiI ACGT 4 cut(s) 155, 261, 282, 445
TaqI TCGA 1 cut(s) 600
TaqII GACCGA 1 cut(s) 492
TasI AATT 3 cut(s) 108, 470, 661
Tru1I TTAA 3 cut(s) 77, 347, 660
Tru9I TTAA 3 cut(s) 77, 347, 660
TscAI CASTG 2 cut(s) 195, 593
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 3 cut(s) 162, 218, 539
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 2 cut(s) 441, 618
TspRI CASTG 2 cut(s) 195, 593
VpaK11BI GGWCC 2 cut(s) 67, 484
VspI ATTAAT 1 cut(s) 660
XbaI TCTAGA 1 cut(s) 460
XmiI GTMKAC 1 cut(s) 314
XspI CTAG 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.