Rroxscaffold_3G00242730

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014746
Physical Location & Seq
Reverse (-)
35196462 .. 35197476
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242730.1

Sequence Viewer

Length: 408 bp
ATGCGCTCGCCTATAACAGCTGCATCTACCGGAGAAGCGCCATGTAGCAATGGAGGGTACGTCAAGGGTGGTGTTACATACGTGATTATGGATAACTTGGTAGTGAAGCCTATGTCTACCATATCAATTTTAGGTGTGCTTAAAGAGTTGAATGTTATTGAGAGAATTCGCGGCCACCCAACCTCACCGGTTGGCTTCGGCGCCGCCGTGGATCATGCCATTGAATCCCACCACAAAGAGGGAAAGATCAAAACCCTCCAGTTATGTTTCGATCCGTCCGATGTTGAGTTTCTTCTTGAGAAGTGGGTTCGGAAATCGGTCAAGAAAGGAGTCGAGGAGCTCGATCTCGACATCTTTCGCGACATCTTTAATTTGAAGGCTCGGTATCGCCGACCACCTTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

135

Amino Acids

15.08

Weight (kDa)

8.93

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 15 - 52 8e-07 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 116
AccII CGCG 2 cut(s) 171, 360
AciI CCGC 2 cut(s) 171, 204
AclWI GGATC 2 cut(s) 219, 266
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 165
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 238
AgeI ACCGGT 1 cut(s) 187
AgsI TTSAA 3 cut(s) 151, 224, 376
AluBI AGCT 2 cut(s) 20, 340
AluI AGCT 2 cut(s) 20, 340
Alw21I GWGCWC 1 cut(s) 342
AlwI GGATC 2 cut(s) 219, 266
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 165
AsiGI ACCGGT 1 cut(s) 187
AspLEI GCGC 3 cut(s) 6, 40, 203
AsuHPI GGTGA 1 cut(s) 177
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 7
BceAI ACGGC 1 cut(s) 191
BfoI RGCGCY 2 cut(s) 41, 204
BisI GCNGC 3 cut(s) 21, 172, 204
BlsI GCNGC 3 cut(s) 22, 173, 205
BmiI GGNNCC 1 cut(s) 202
BmsI GCATC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 207, 398
BsaWI WCCGGW 2 cut(s) 29, 187
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 55
BseDI CCNNGG 2 cut(s) 207, 398
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 55
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 2 cut(s) 171, 360
BshFI GGCC 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 2 cut(s) 30, 188
BslI CCNNNNNNNGG 1 cut(s) 238
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 4 cut(s) 211, 246, 271, 343
Bsp68I TCGCGA 1 cut(s) 360
BspACI CCGC 2 cut(s) 171, 204
BspANI GGCC 1 cut(s) 174
BspFNI CGCG 2 cut(s) 171, 360
BspLI GGNNCC 1 cut(s) 202
BspPI GGATC 2 cut(s) 219, 266
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 2 cut(s) 207, 398
BssMI GATC 4 cut(s) 211, 246, 271, 343
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 8
BstDSI CCRYGG 1 cut(s) 207
BstFNI CGCG 2 cut(s) 171, 360
BstH2I RGCGCY 2 cut(s) 41, 204
BstHHI GCGC 3 cut(s) 6, 40, 203
BstKTI GATC 4 cut(s) 214, 249, 274, 346
BstMBI GATC 4 cut(s) 211, 246, 271, 343
BstUI CGCG 2 cut(s) 171, 360
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 207
BtuMI TCGCGA 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 8
CfoI GCGC 3 cut(s) 6, 40, 203
Cfr10I RCCGGY 1 cut(s) 187
Csp6I GTAC 1 cut(s) 58
CspAI ACCGGT 1 cut(s) 187
CviAII CATG 2 cut(s) 42, 215
CviJI RGCY 6 cut(s) 20, 109, 174, 195, 340, 380
CviKI_1 RGCY 6 cut(s) 20, 109, 174, 195, 340, 380
CviQI GTAC 1 cut(s) 58
DinI GGCGCC 1 cut(s) 202
DpnI GATC 4 cut(s) 213, 248, 273, 345
DpnII GATC 4 cut(s) 211, 246, 271, 343
EaeI YGGCCR 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 2 cut(s) 45, 218
FaiI YATR 8 cut(s) 14, 43, 79, 89, 113, 122, 216, 265
FatI CATG 2 cut(s) 41, 214
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 3 cut(s) 21, 172, 204
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 3 cut(s) 21, 172, 204
GlaI GCGC 3 cut(s) 5, 39, 202
GluI GCNGC 3 cut(s) 21, 172, 204
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 2 cut(s) 41, 204
HaeIII GGCC 1 cut(s) 174
HapII CCGG 2 cut(s) 30, 188
HhaI GCGC 3 cut(s) 6, 40, 203
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 2 cut(s) 45, 218
Hin6I GCGC 3 cut(s) 4, 38, 201
HinP1I GCGC 3 cut(s) 4, 38, 201
HinfI GANTC 2 cut(s) 224, 330
HpaII CCGG 2 cut(s) 30, 188
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 2 cut(s) 280, 312
Hpy188III TCNNGA 4 cut(s) 296, 322, 347, 359
Hpy8I GTNNAC 1 cut(s) 117
HpyAV CCTTC 1 cut(s) 370
HpyCH4IV ACGT 2 cut(s) 60, 81
HpyCH4V TGCA 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 60, 81
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 2 cut(s) 45, 218
HspAI GCGC 3 cut(s) 4, 38, 201
KasI GGCGCC 1 cut(s) 200
Kzo9I GATC 4 cut(s) 211, 246, 271, 343
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 3 cut(s) 43, 201, 272
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 32
MaeII ACGT 2 cut(s) 60, 81
MaeIII GTNAC 1 cut(s) 73
MalI GATC 4 cut(s) 213, 248, 273, 345
MboI GATC 4 cut(s) 211, 246, 271, 343
MboII GAAGA 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 3 cut(s) 126, 165, 370
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 1 cut(s) 339
MnlI CCTC 5 cut(s) 47, 193, 232, 266, 328
MseI TTAA 2 cut(s) 141, 369
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 30, 188
MvnI CGCG 2 cut(s) 171, 360
NarI GGCGCC 1 cut(s) 201
NdeII GATC 4 cut(s) 211, 246, 271, 343
NlaIII CATG 2 cut(s) 45, 218
NlaIV GGNNCC 1 cut(s) 202
NruI TCGCGA 1 cut(s) 360
PcsI WCGNNNNNNNCGW 4 cut(s) 204, 276, 339, 388
PfeI GAWTC 1 cut(s) 224
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 3 cut(s) 22, 173, 205
PleI GAGTC 1 cut(s) 338
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 338
Ppu21I YACGTR 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 20
RruI TCGCGA 1 cut(s) 360
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 141, 369
SatI GCNGC 3 cut(s) 21, 172, 204
Sau3AI GATC 4 cut(s) 211, 246, 271, 343
SchI GAGTC 1 cut(s) 339
SduI GDGCHC 1 cut(s) 342
SetI ASST 7 cut(s) 22, 63, 84, 136, 185, 342, 400
SfaNI GCATC 1 cut(s) 32
SfoI GGCGCC 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 3 cut(s) 126, 165, 370
SsiI CCGC 2 cut(s) 171, 204
SspDI GGCGCC 1 cut(s) 200
SstI GAGCTC 1 cut(s) 342
StyI CCWWGG 1 cut(s) 398
TaiI ACGT 2 cut(s) 63, 84
TaqI TCGA 4 cut(s) 270, 333, 342, 348
TaqII GACCGA 1 cut(s) 307
TasI AATT 3 cut(s) 126, 165, 370
TauI GCSGC 2 cut(s) 174, 206
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 2 cut(s) 141, 369
Tru9I TTAA 2 cut(s) 141, 369
TseI GCWGC 1 cut(s) 20
TspGWI ACGGA 1 cut(s) 264
XapI RAATTY 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.