FvH4_5g22370

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005057
Physical Location & Seq
Reverse (-)
13754335 .. 13762357
8023 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22370.t2

Sequence Viewer

Length: 1395 bp
ATGGATTCGTTTCCCAAGTCTCTCAATCCAAACAAACGACTCAAAGAAGAATTCGTCAGCAATCTGACTGGAACTTCCATGCTCGAAATCGCCGCCATCACCACAATAATCCCTCTTCTGTTCCTGGTGCGGCGATCATTGGGTTTCATGCATCCTTCTGATGTTGTTACATTGAAGAAGGATGATGATGCAAAAGTTGGTGCTAAGAGTTGGCAGGCTTACATGGCTGCACTGTCTGTGGATTTTCTATTCATAATGGTTCCCTTCGTCTTATTTTTGACTGTTCTAGCAGATTGGATGTATATCTGGACAACTTTGCTAACATTGTTAGTTGTTTTCAGCTTTGCAGCCAAAAGATTTGGTTTTGGTTCTACTTTGAAAGAAGAACCCGATTCTGTTAGGAAAACTATTACATCACATAGGGTTGTAATGATGATCCTAACATGCTTATGCATCTTGGCTGTTGACTTCAAGATTTTTCCTAGAAGATATGCTAAGACTGAAACTTATGGTACTGGCTTGATGGATTTCGGAGTTGGCTCATTTGTTGTAATAAACTCATTTGTGTCTCGGCAAGCACGAAATCTGTCGTGGGAGAAATGGAAGACTGCTATAAAATCTACTACTCCGCTAATCATCCTTGGGTTAGCTCGCATTCTCTCTACTGCAGGTGTAGACTATCAGGTTCATGCAGCTGAATATGGAGTACACTGGAATTTCTTTTTCACAATGGCTGCCATTTCATTTCTTACATCCATAATCAATATGCCCGCACAATATTCTGGAATTCTCGGTGCACTAATTCTAGTAGGTTACGAGGGTTGGTTGGTGCAGGGGTTGAACATGTATCTGCTGTCTAATGACAGGGGAAGCGACATAATTAGCCAAAATAAGGAGGGATTGTTTAGCATATTTGGTTATTGGAGCATGTACCTCATTGGCGTCCAGGTGGGGAATTTTCTGTTTTTCAGTAACCGTTCTTCTGCTACAACAAGTAGAAAGAAATCGACAAGGAATAGAGCCGCAATACTTTCACTTCTGTTTTGGCTAGTAACTCTGGTTGTAGATAGCCATGTCGAGAGAGTTTCTCGTAGATCGTGCAATCTTCCTTATGTTACACTGGTATTGGCAACAAATCTACAGGGTCTGGCAATAGTTTTGGTCTCTGATTCTCTCCCTGGAACCAAAACATCAACAATTGAAGAAGCATATAACCGCAATTTGCTAGCAATATTTCTTCTGGCAAATTTGCTTACAGGTCTGATAAATTTTTTGGTGGATACCCTGTTTGCATCAACAGTCAAAGCTCTTTCTGTGATGATTGCCTATGCATTCATGTTAACTTTCATTCCTGGATTGCTTGACTTTTGTGGCATCCGAGTTAAATTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

465

Amino Acids

51.91

Weight (kDa)

9.47

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 12 - 451 6.8e-131 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 659
Acc36I ACCTGC 1 cut(s) 659
AccI GTMKAC 1 cut(s) 675
AciI CCGC 6 cut(s) 93, 130, 629, 771, 1023, 1216
AclWI GGATC 1 cut(s) 430
AcsI RAATTY 7 cut(s) 50, 715, 786, 955, 1246, 1267, 1385
AcyI GRCGYC 1 cut(s) 942
AfaI GTAC 3 cut(s) 514, 708, 932
AfiI CCNNNNNNNGG 1 cut(s) 892
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 5 cut(s) 175, 379, 472, 841, 1202
AjnI CCWGG 4 cut(s) 123, 945, 1177, 1351
AluBI AGCT 4 cut(s) 342, 650, 695, 1307
AluI AGCT 4 cut(s) 342, 650, 695, 1307
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 3 cut(s) 24, 573, 1168
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 1147
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 4 cut(s) 227, 347, 692, 734
ApoI RAATTY 7 cut(s) 50, 715, 786, 955, 1246, 1267, 1385
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 1225
BaeGI GKGCMC 1 cut(s) 799
BarI GAAGNNNNNNTAC 2 cut(s) 964, 996
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 4 cut(s) 214, 359, 704, 721
BccI CCATC 2 cut(s) 104, 517
BciT130I CCWGG 4 cut(s) 125, 947, 1179, 1353
BciVI GTATCC 1 cut(s) 1273
BcoDI GTCTC 3 cut(s) 24, 573, 1168
BfaI CTAG 5 cut(s) 287, 483, 806, 1049, 1226
BfmI CTRYAG 2 cut(s) 666, 1139
BfuAI ACCTGC 1 cut(s) 659
BfuI GTATCC 1 cut(s) 1273
BisI GCNGC 7 cut(s) 93, 131, 228, 348, 693, 735, 1023
BlsI GCNGC 7 cut(s) 94, 132, 229, 349, 694, 736, 1024
Bme1390I CCNGG 4 cut(s) 125, 947, 1179, 1353
BmiI GGNNCC 2 cut(s) 261, 1183
BmrFI CCNGG 4 cut(s) 125, 947, 1179, 1353
BmsI GCATC 5 cut(s) 160, 178, 462, 1301, 1383
BmtI GCTAGC 1 cut(s) 1229
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 1072, 1104
BsaBI GATNNNNATC 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 942
BsaI GGTCTC 1 cut(s) 1168
BsaJI CCNNGG 2 cut(s) 640, 1177
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse1I ACTGG 4 cut(s) 73, 520, 716, 1125
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 4 cut(s) 125, 947, 1179, 1353
BseDI CCNNGG 2 cut(s) 640, 1177
BseGI GGATG 6 cut(s) 151, 187, 303, 636, 752, 1374
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 892
BseNI ACTGG 4 cut(s) 73, 520, 716, 1125
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 4 cut(s) 214, 359, 704, 721
BsgI GTGCAG 2 cut(s) 213, 851
BsiHKAI GWGCWC 1 cut(s) 799
BslI CCNNNNNNNGG 1 cut(s) 892
BsmAI GTCTC 3 cut(s) 24, 573, 1168
BsmI GAATGC 2 cut(s) 654, 1331
Bso31I GGTCTC 1 cut(s) 1168
Bsp1286I GDGCHC 1 cut(s) 799
Bsp143I GATC 3 cut(s) 134, 435, 1094
BspACI CCGC 6 cut(s) 93, 130, 629, 771, 1023, 1216
BspLI GGNNCC 2 cut(s) 261, 1183
BspMAI CTGCAG 1 cut(s) 670
BspMI ACCTGC 1 cut(s) 659
BspOI GCTAGC 1 cut(s) 1229
BspPI GGATC 1 cut(s) 430
BspTNI GGTCTC 1 cut(s) 1168
BsrI ACTGG 4 cut(s) 73, 520, 716, 1125
BssECI CCNNGG 2 cut(s) 640, 1177
BssMI GATC 3 cut(s) 134, 435, 1094
BssNI GRCGYC 1 cut(s) 942
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 4 cut(s) 125, 947, 1179, 1353
Bst4CI ACNGT 4 cut(s) 234, 283, 977, 1300
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 942
BstC8I GCNNGC 5 cut(s) 216, 576, 652, 771, 1227
BstDEI CTNAG 2 cut(s) 204, 495
BstF5I GGATG 6 cut(s) 151, 187, 303, 636, 752, 1374
BstKTI GATC 3 cut(s) 137, 438, 1097
BstMAI GTCTC 3 cut(s) 24, 573, 1168
BstMBI GATC 3 cut(s) 134, 435, 1094
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 4 cut(s) 125, 947, 1179, 1353
BstNSI RCATGY 3 cut(s) 447, 847, 931
BstSCI CCNGG 4 cut(s) 123, 945, 1177, 1351
BstSFI CTRYAG 2 cut(s) 666, 1139
BstSLI GKGCMC 1 cut(s) 799
BstV1I GCAGC 4 cut(s) 214, 359, 704, 721
BstV2I GAAGAC 1 cut(s) 611
BsuI GTATCC 1 cut(s) 1273
BtsCI GGATG 6 cut(s) 151, 187, 303, 636, 752, 1374
BtsIMutI CAGTG 3 cut(s) 230, 709, 1118
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 5 cut(s) 216, 576, 652, 771, 1227
CaiI CAGNNNCTG 1 cut(s) 1147
CseI GACGC 1 cut(s) 931
Csp6I GTAC 3 cut(s) 513, 707, 931
CviAII CATG 9 cut(s) 79, 148, 223, 444, 689, 844, 928, 1073, 1336
CviQI GTAC 3 cut(s) 513, 707, 931
DdeI CTNAG 2 cut(s) 204, 495
DpnI GATC 3 cut(s) 136, 437, 1096
DpnII GATC 3 cut(s) 134, 435, 1094
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 640
Eco31I GGTCTC 1 cut(s) 1168
EcoRI GAATTC 2 cut(s) 50, 786
EcoRII CCWGG 4 cut(s) 123, 945, 1177, 1351
EcoT14I CCWWGG 1 cut(s) 640
EcoT22I ATGCAT 3 cut(s) 153, 455, 1333
ErhI CCWWGG 1 cut(s) 640
FaeI CATG 9 cut(s) 82, 151, 226, 447, 692, 847, 931, 1076, 1339
FatI CATG 9 cut(s) 78, 147, 222, 443, 688, 843, 927, 1072, 1335
FauI CCCGC 1 cut(s) 778
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 7 cut(s) 93, 131, 228, 348, 693, 735, 1023
FokI GGATG 6 cut(s) 138, 194, 310, 623, 739, 1361
Fsp4HI GCNGC 7 cut(s) 93, 131, 228, 348, 693, 735, 1023
FspBI CTAG 5 cut(s) 287, 483, 806, 1049, 1226
GluI GCNGC 7 cut(s) 93, 131, 228, 348, 693, 735, 1023
HgaI GACGC 1 cut(s) 931
Hin1I GRCGYC 1 cut(s) 942
Hin1II CATG 9 cut(s) 82, 151, 226, 447, 692, 847, 931, 1076, 1339
HincII GTYRAC 2 cut(s) 466, 1341
HindII GTYRAC 2 cut(s) 466, 1341
HinfI GANTC 4 cut(s) 5, 39, 392, 1169
HpaI GTTAAC 1 cut(s) 1341
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 5 cut(s) 466, 676, 709, 797, 1341
Hpy188I TCNGA 6 cut(s) 66, 160, 533, 1168, 1263, 1379
Hpy188III TCNNGA 4 cut(s) 307, 472, 783, 1078
Hpy8I GTNNAC 5 cut(s) 466, 676, 709, 797, 1341
HpyAV CCTTC 3 cut(s) 165, 172, 274
HpyCH4III ACNGT 4 cut(s) 234, 283, 977, 1300
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 2 cut(s) 204, 495
Hsp92I GRCGYC 1 cut(s) 942
Hsp92II CATG 9 cut(s) 82, 151, 226, 447, 692, 847, 931, 1076, 1339
KspAI GTTAAC 1 cut(s) 1341
Kzo9I GATC 3 cut(s) 134, 435, 1094
LmnI GCTCC 1 cut(s) 924
Lsp1109I GCAGC 4 cut(s) 214, 359, 704, 721
LweI GCATC 5 cut(s) 160, 178, 462, 1301, 1383
MaeI CTAG 5 cut(s) 287, 483, 806, 1049, 1226
MaeIII GTNAC 5 cut(s) 166, 812, 971, 1051, 1114
MalI GATC 3 cut(s) 136, 437, 1096
MboI GATC 3 cut(s) 134, 435, 1094
MfeI CAATTG 1 cut(s) 1197
MhlI GDGCHC 1 cut(s) 799
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 4 cut(s) 123, 811, 889, 944
Mph1103I ATGCAT 3 cut(s) 153, 455, 1333
MseI TTAA 2 cut(s) 1340, 1383
MslI CAYNNNNRTG 1 cut(s) 448
MspA1I CMGCKG 1 cut(s) 695
MspR9I CCNGG 4 cut(s) 125, 947, 1179, 1353
MunI CAATTG 1 cut(s) 1197
Mva1269I GAATGC 2 cut(s) 654, 1331
MvaI CCWGG 4 cut(s) 125, 947, 1179, 1353
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeII GATC 3 cut(s) 134, 435, 1094
NheI GCTAGC 1 cut(s) 1225
NlaIII CATG 9 cut(s) 82, 151, 226, 447, 692, 847, 931, 1076, 1339
NlaIV GGNNCC 2 cut(s) 261, 1183
NmeAIII GCCGAG 1 cut(s) 550
NsiI ATGCAT 3 cut(s) 153, 455, 1333
NspI RCATGY 3 cut(s) 447, 847, 931
PaqCI CACCTGC 1 cut(s) 659
PciI ACATGT 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 577
PctI GAATGC 2 cut(s) 654, 1331
PfeI GAWTC 3 cut(s) 5, 392, 1169
PfoI TCCNGGA 1 cut(s) 1351
PkrI GCNGC 7 cut(s) 94, 132, 229, 349, 694, 736, 1024
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PscI ACATGT 1 cut(s) 843
Psp6I CCWGG 4 cut(s) 123, 945, 1177, 1351
PspGI CCWGG 4 cut(s) 123, 945, 1177, 1351
PspN4I GGNNCC 2 cut(s) 261, 1183
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 1147
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 3 cut(s) 514, 708, 932
RsaNI GTAC 3 cut(s) 513, 707, 931
RseI CAYNNNNRTG 1 cut(s) 448
SaqAI TTAA 2 cut(s) 1340, 1383
SatI GCNGC 7 cut(s) 93, 131, 228, 348, 693, 735, 1023
Sau3AI GATC 3 cut(s) 134, 435, 1094
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 4 cut(s) 125, 947, 1179, 1353
SduI GDGCHC 1 cut(s) 799
SfaNI GCATC 5 cut(s) 160, 178, 462, 1301, 1383
SfcI CTRYAG 2 cut(s) 666, 1139
SmiMI CAYNNNNRTG 1 cut(s) 448
SsiI CCGC 6 cut(s) 93, 130, 629, 771, 1023, 1216
SspI AATATT 2 cut(s) 779, 1233
SspMI CTAG 5 cut(s) 287, 483, 806, 1049, 1226
StyD4I CCNGG 4 cut(s) 123, 945, 1177, 1351
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 4 cut(s) 234, 283, 977, 1300
TaqI TCGA 3 cut(s) 84, 1007, 1077
TatI WGTACW 1 cut(s) 706
TauI GCSGC 3 cut(s) 95, 133, 1025
TfiI GAWTC 3 cut(s) 5, 392, 1169
Tru1I TTAA 2 cut(s) 1340, 1383
Tru9I TTAA 2 cut(s) 1340, 1383
TscAI CASTG 3 cut(s) 237, 716, 1125
TseI GCWGC 4 cut(s) 227, 347, 692, 734
TspDTI ATGAA 6 cut(s) 136, 241, 677, 732, 1324, 1336
TspRI CASTG 3 cut(s) 237, 716, 1125
VneI GTGCAC 1 cut(s) 795
XapI RAATTY 7 cut(s) 50, 715, 786, 955, 1246, 1267, 1385
XceI RCATGY 3 cut(s) 447, 847, 931
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 5 cut(s) 287, 483, 806, 1049, 1226
Zsp2I ATGCAT 3 cut(s) 153, 455, 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.