MD06G1036000.v1.1

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005057
Physical Location & Seq
Reverse (-)
4651894 .. 4655813
3920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1036000.v1.1.491

Sequence Viewer

Length: 933 bp
ATGATTGTGGTGGTGCTGATTTTCAGATCAGCTTTGGTGGCTGTCGAAGAGAAGAAGAAAATGGGTTGGTGCAGGTGGAGGAAAGGGAAGTCCCGCATCCCTAGGTCATGGAAGACTGCTATTCAATCTGCTAGTCCACTAATCATCCTTGGGTTTGCCCGTCTTCTCTCTACCACAGGTGTGGACTATCAGGTAGGTTTATCATGTTATCAGGTCAATTTGCTTCATGTGGCGGAATATGGAGTGCACTGGAATTTCTTCTTCACGCTTGCTGCGATATCAATTCTTACATCCATAATTAATATTCCCACGCAGTATTCTGGCATTCTTGGCTCACTAATTCTAGTAGGTTACCAAGTTTGCTTGATGCATGGGTTAAACTCGTATCTGCTTTCTAATGAAAGGGGAACTGATATAATCAGCCAAAATAAGGAGGGGTTTTTTAGCATATTTGGATATTGGGGCATGTACCTTGTTGGTGTCCAATTGGGAAACTTTCTTCTATTCAATTACCATTCCTCTGCTACAATGGGAAGCAATAAATGGGCAACAATTAGAGTTTGGATACTTTCACTTCTCCTTTGGTTATTAACTGTGATTATAGACCGCCATGTAGAGAGAGTTTCCCGTCGGATGTGCAACCTGGCTTATGTTACACTGGTTTTGGCCGTAAATTTACAGGTGTTGGCAATATTTCTTCTCTCTGATTTTCTCCCCGGAAGCAAAACTTCAGTGCTGGAAGAAGCATATAACCGCAATTTGCTGGGGACATTTCTTCTGGCGAATTTGCTCACGGGTTTGGTGAACTTGATGGTGGAGACCCTGTTTGCATCGGCAGTCAAAGCCCTTTTTATTTTAATTGCCTATACATTTTCGACGACCTTCATCATTGGACTGCTAGATTACTGCGGCATCCGGTTGAAATTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

311

Amino Acids

34.94

Weight (kDa)

9.53

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 5 - 298 2.1e-77 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 63
AciI CCGC 5 cut(s) 94, 233, 607, 754, 909
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 4 cut(s) 253, 673, 784, 923
AcuI CTGAAG 1 cut(s) 714
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 3 cut(s) 125, 508, 922
AjnI CCWGG 1 cut(s) 642
AleI CACNNNNGTG 1 cut(s) 179
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 812
Alw44I GTGCAC 1 cut(s) 245
AoxI GGCC 1 cut(s) 666
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 4 cut(s) 253, 673, 784, 923
AseI ATTAAT 1 cut(s) 300
Asp700I GAANNNNTTC 1 cut(s) 257
AspA2I CCTAGG 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 1 cut(s) 814
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 249
BbsI GAAGAC 2 cut(s) 119, 155
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 259
BccI CCATC 1 cut(s) 805
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 1 cut(s) 644
BciVI GTATCC 1 cut(s) 558
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 4 cut(s) 102, 132, 344, 899
BfuAI ACCTGC 1 cut(s) 63
BfuI GTATCC 1 cut(s) 558
BisI GCNGC 2 cut(s) 273, 910
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 2 cut(s) 274, 911
Bme1390I CCNGG 2 cut(s) 644, 717
BmrFI CCNGG 2 cut(s) 644, 717
BmsI GCATC 4 cut(s) 105, 357, 839, 921
BpiI GAAGAC 2 cut(s) 119, 155
BpuMI CCSGG 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 812
BsaJI CCNNGG 3 cut(s) 101, 148, 715
BsaWI WCCGGW 1 cut(s) 915
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 2 cut(s) 254, 663
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 3 cut(s) 101, 148, 715
BseGI GGATG 5 cut(s) 96, 144, 290, 639, 912
BseLI CCNNNNNNNGG 1 cut(s) 430
BseNI ACTGG 2 cut(s) 254, 663
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 1 cut(s) 259
BseYI CCCAGC 1 cut(s) 763
BsgI GTGCAG 1 cut(s) 91
BshFI GGCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 2 cut(s) 717, 916
BslFI GGGAC 2 cut(s) 76, 781
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 812
BsmFI GGGAC 2 cut(s) 76, 781
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 1 cut(s) 668
Bso31I GGTCTC 1 cut(s) 812
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 1 cut(s) 26
BspACI CCGC 5 cut(s) 94, 233, 607, 754, 909
BspANI GGCC 1 cut(s) 668
BspMI ACCTGC 1 cut(s) 63
BspTNI GGTCTC 1 cut(s) 812
BsrI ACTGG 2 cut(s) 254, 663
BssECI CCNNGG 3 cut(s) 101, 148, 715
BssMI GATC 1 cut(s) 26
BssT1I CCWWGG 2 cut(s) 101, 148
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 270
BstEII GGTNACC 1 cut(s) 350
BstF5I GGATG 5 cut(s) 96, 144, 290, 639, 912
BstKTI GATC 1 cut(s) 29
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 38, 330, 842
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 469
BstPI GGTNACC 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 642, 715
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 2 cut(s) 119, 155
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 1 cut(s) 668
BtsCI GGATG 5 cut(s) 96, 144, 290, 639, 912
BtsIMutI CAGTG 3 cut(s) 247, 656, 738
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 270
Csp6I GTAC 1 cut(s) 469
CviAII CATG 6 cut(s) 108, 204, 227, 371, 466, 611
CviJI RGCY 7 cut(s) 32, 41, 333, 423, 647, 668, 845
CviKI_1 RGCY 7 cut(s) 32, 41, 333, 423, 647, 668, 845
CviQI GTAC 1 cut(s) 469
DpnI GATC 1 cut(s) 28
DpnII GATC 1 cut(s) 26
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 2 cut(s) 101, 148
Eco31I GGTCTC 1 cut(s) 812
Eco32I GATATC 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 714
Eco91I GGTNACC 1 cut(s) 350
EcoO65I GGTNACC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 642
EcoRV GATATC 1 cut(s) 279
EcoT14I CCWWGG 2 cut(s) 101, 148
EcoT22I ATGCAT 1 cut(s) 372
ErhI CCWWGG 2 cut(s) 101, 148
FaeI CATG 6 cut(s) 111, 207, 230, 374, 469, 614
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FaqI GGGAC 2 cut(s) 76, 781
FatI CATG 6 cut(s) 107, 203, 226, 370, 465, 610
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 2 cut(s) 273, 910
FokI GGATG 5 cut(s) 83, 131, 277, 646, 899
Fsp4HI GCNGC 2 cut(s) 273, 910
FspBI CTAG 4 cut(s) 102, 132, 344, 899
GluI GCNGC 2 cut(s) 273, 910
GsaI CCCAGC 1 cut(s) 767
HaeIII GGCC 1 cut(s) 668
HapII CCGG 2 cut(s) 717, 916
Hin1II CATG 6 cut(s) 111, 207, 230, 374, 469, 614
HpaII CCGG 2 cut(s) 717, 916
HphI GGTGA 1 cut(s) 814
Hpy166II GTNNAC 4 cut(s) 137, 184, 247, 805
Hpy188I TCNGA 3 cut(s) 26, 633, 706
Hpy8I GTNNAC 4 cut(s) 137, 184, 247, 805
Hpy99I CGWCG 2 cut(s) 633, 880
HpyAV CCTTC 1 cut(s) 892
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4V TGCA 5 cut(s) 72, 247, 370, 639, 830
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 330, 842
Hsp92II CATG 6 cut(s) 111, 207, 230, 374, 469, 614
Kzo9I GATC 1 cut(s) 26
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 4 cut(s) 105, 357, 839, 921
MaeI CTAG 4 cut(s) 102, 132, 344, 899
MaeIII GTNAC 2 cut(s) 350, 652
MalI GATC 1 cut(s) 28
MboI GATC 1 cut(s) 26
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 611
MnlI CCTC 3 cut(s) 72, 427, 529
Mph1103I ATGCAT 1 cut(s) 372
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 4 cut(s) 300, 377, 590, 857
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 2 cut(s) 717, 916
MspR9I CCNGG 2 cut(s) 644, 717
MunI CAATTG 1 cut(s) 485
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 1 cut(s) 644
MwoI GCNNNNNNNGC 3 cut(s) 38, 330, 842
NciI CCSGG 1 cut(s) 717
NdeII GATC 1 cut(s) 26
NlaIII CATG 6 cut(s) 111, 207, 230, 374, 469, 614
NsiI ATGCAT 1 cut(s) 372
NspI RCATGY 1 cut(s) 469
OliI CACNNNNGTG 1 cut(s) 179
PaqCI CACCTGC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 272
PctI GAATGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 274, 911
PshBI ATTAAT 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 642
PspEI GGTNACC 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 763
PspGI CCWGG 1 cut(s) 642
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 4 cut(s) 300, 377, 590, 857
SatI GCNGC 2 cut(s) 273, 910
Sau3AI GATC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 644, 717
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 4 cut(s) 105, 357, 839, 921
SmiMI CAYNNNNRTG 1 cut(s) 179
SsiI CCGC 5 cut(s) 94, 233, 607, 754, 909
SspI AATATT 2 cut(s) 304, 693
SspMI CTAG 4 cut(s) 102, 132, 344, 899
StyD4I CCNGG 2 cut(s) 642, 715
StyI CCWWGG 2 cut(s) 101, 148
TaaI ACNGT 1 cut(s) 595
TaqI TCGA 2 cut(s) 45, 875
TauI GCSGC 1 cut(s) 912
Tru1I TTAA 4 cut(s) 300, 377, 590, 857
Tru9I TTAA 4 cut(s) 300, 377, 590, 857
TscAI CASTG 3 cut(s) 254, 663, 738
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 3 cut(s) 215, 414, 874
TspRI CASTG 3 cut(s) 254, 663, 738
VneI GTGCAC 1 cut(s) 245
VspI ATTAAT 1 cut(s) 300
XapI RAATTY 4 cut(s) 253, 673, 784, 923
XceI RCATGY 1 cut(s) 469
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 4 cut(s) 102, 132, 344, 899
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.