RLG00000003064

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005057
Physical Location & Seq
Forward (+)
43858871 .. 43866296
7426 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003064

Sequence Viewer

Length: 1398 bp
ATGGATTCTTCAGTTCCCAAGTCTTTCAATCCAAACAGACGTCTCAAGGAAGAATTCGTCAGCAATTTGACTGGATCTTCTATGCTGGAAATCGCCGCCATCACCACAATAATCCCAGCTCTGTTCCTGCTGCGGCGAGCAGTCGGCTTCAATCGTCCTGCTGATGATGTCACATTGAAGAAAAATGATGATGCAAAAGTTGGTTCTAAGAGTTGGCAGGCTTACATGGCTAGGCTGGCTGTGGATTTTCTATTCATTATGGTTCCATTTGTCTTAATTTTCACTGTCTTATCAGAGTGGATGTATATCTGGGCAACTTTGCTAATTCTGTTACTTGTTTTCAGCATTGCAGCCAGGAGTTTTGGTTTTGGTTCTACTTTGGAAGAAGAACCCGATTCTATTAGGACAAGTATTACATCATTTAGGGTTGCAACGATGATGCTAACATGCATATGTATCTTGGCTGTTGACTTCACAATTTTTCCTAGAAGATATGCCAAGACTGAGACTTATGGTACTGGCTGGATGGATCTTGGGGTTGGCTCATTTGTCGTAGCAAACTCATTTGTGTCTCGGCAAGCACGAAATTTATCATGGACGAAATGGAAGACTGCAATTCAATCTACTAGTCCACTAATCATCCTTGGGTTTGTTCGTCTTCTCTCTACTGCAGGTCTAGACTATCAGCATCACGCAGCTGAATATGGAGTACACTGGAATTTCTTTTTCACACTTGCTGCCATATCATTTCTTACATCCATAATTAATGTGCCCCCGCAGTATTCTGGAATTCTTGGTGCACTAATTCTAGTAGGTTACGAAGGTTGGTTGATGCAGGGGTTAAACATGTATCTGCTTTCTAATGAAAGGGGAAGTGATATAATTAGCCAAAATAAGGAGGGATTTTTTAGCATATTTGGATATTGGAGCATGTACCTCATTGGTGTCCAGGTGGGCAATTTTCTTTTTTTCAGTAACCACTCCTCTGCTACAACAAGAAGCCATAAATGGACAAGGAATAGAGCTGCAATACTTTCACTTCTGTTTTGGCTAGTAACTGTGGTTATAGATAGCCATGTCCAGAGAGTTTCCCGTAGAATGTGCAATCTGGCTTATGTTACACTGGTATTGGCGGTAAATCTAGAGGGTATGGCCATAGTTCTGTTCTCTGATTGTTTCCCTGGAAGCAAAACATCAGTACTTGAAGAAGCATATAACCGCAATTTGCTAGCAATATTTCTTTTGGCAAATTTGCTTACTGGTCTGGTAAACTTTTTGGTGGATACCCTGTTTGCATCATTAGTCAAAGCTCTTTCTGTGATGATTGCGTATGCATTCACGTTAACTTTCATTCCTGGATTGCTGGACTTTTGCGGCATCCGATTTAGATTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

466

Amino Acids

52.26

Weight (kDa)

9.12

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 13 - 452 1.9e-130 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 662
AciI CCGC 6 cut(s) 96, 133, 776, 1133, 1219, 1374
AclWI GGATC 2 cut(s) 82, 537
AcoI YGGCCR 1 cut(s) 1152
AcsI RAATTY 5 cut(s) 53, 586, 718, 789, 1249
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 4 cut(s) 517, 711, 935, 1200
AfiI CCNNNNNNNGG 1 cut(s) 895
AflIII ACRYGT 1 cut(s) 846
AgsI TTSAA 5 cut(s) 28, 151, 178, 620, 1205
AhlI ACTAGT 1 cut(s) 626
AjnI CCWGG 4 cut(s) 353, 948, 1180, 1354
AluBI AGCT 4 cut(s) 119, 698, 1025, 1310
AluI AGCT 4 cut(s) 119, 698, 1025, 1310
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 3 cut(s) 47, 500, 576
Alw44I GTGCAC 1 cut(s) 798
AlwI GGATC 2 cut(s) 82, 537
AoxI GGCC 1 cut(s) 1152
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 5 cut(s) 130, 350, 695, 737, 1025
ApoI RAATTY 5 cut(s) 53, 586, 718, 789, 1249
AseI ATTAAT 1 cut(s) 765
AsuHPI GGTGA 1 cut(s) 94
AsuNHI GCTAGC 1 cut(s) 1228
BaeGI GKGCMC 2 cut(s) 774, 802
BalI TGGCCA 1 cut(s) 1154
BbsI GAAGAC 2 cut(s) 614, 650
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 5 cut(s) 117, 362, 707, 724, 1012
BccI CCATC 2 cut(s) 107, 520
BciT130I CCWGG 4 cut(s) 355, 950, 1182, 1356
BciVI GTATCC 1 cut(s) 1276
BcoDI GTCTC 3 cut(s) 47, 500, 576
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 8 cut(s) 231, 486, 627, 677, 809, 1052, 1142, 1229
BfmI CTRYAG 1 cut(s) 669
BfuAI ACCTGC 1 cut(s) 662
BfuI GTATCC 1 cut(s) 1276
BisI GCNGC 8 cut(s) 96, 131, 134, 351, 696, 738, 1026, 1375
BlsI GCNGC 8 cut(s) 97, 132, 135, 352, 697, 739, 1027, 1376
BmcAI AGTACT 1 cut(s) 1200
Bme1390I CCNGG 4 cut(s) 355, 950, 1182, 1356
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 4 cut(s) 355, 950, 1182, 1356
BmsI GCATC 6 cut(s) 181, 429, 697, 822, 1304, 1386
BmtI GCTAGC 1 cut(s) 1232
BpiI GAAGAC 2 cut(s) 614, 650
BpuEI CTTGAG 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 643, 1180
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 895
Bse1I ACTGG 5 cut(s) 76, 523, 719, 1128, 1264
Bse3DI GCAATG 1 cut(s) 345
Bse8I GATNNNNATC 1 cut(s) 305
BseBI CCWGG 4 cut(s) 355, 950, 1182, 1356
BseDI CCNNGG 2 cut(s) 643, 1180
BseGI GGATG 5 cut(s) 306, 531, 639, 755, 1377
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 895
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 5 cut(s) 76, 523, 719, 1128, 1264
BseRI GAGGAG 1 cut(s) 973
BseSI GKGCMC 2 cut(s) 774, 802
BseXI GCAGC 5 cut(s) 117, 362, 707, 724, 1012
BseYI CCCAGC 1 cut(s) 115
BshFI GGCC 1 cut(s) 1154
BsiHKAI GWGCWC 1 cut(s) 802
BslI CCNNNNNNNGG 1 cut(s) 895
BsmAI GTCTC 3 cut(s) 47, 500, 576
BsmBI CGTCTC 1 cut(s) 47
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 1 cut(s) 1154
Bsp1286I GDGCHC 2 cut(s) 774, 802
Bsp143I GATC 2 cut(s) 74, 529
BspACI CCGC 6 cut(s) 96, 133, 776, 1133, 1219, 1374
BspANI GGCC 1 cut(s) 1154
BspCNI CTCAG 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 264
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 662
BspOI GCTAGC 1 cut(s) 1232
BspPI GGATC 2 cut(s) 82, 537
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 5 cut(s) 76, 523, 719, 1128, 1264
BssECI CCNNGG 2 cut(s) 643, 1180
BssMI GATC 2 cut(s) 74, 529
BssNI GRCGYC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 643
Bst2UI CCWGG 4 cut(s) 355, 950, 1182, 1356
Bst4CI ACNGT 2 cut(s) 286, 1060
BstACI GRCGYC 1 cut(s) 40
BstC8I GCNNGC 5 cut(s) 138, 219, 237, 579, 1230
BstDEI CTNAG 2 cut(s) 207, 504
BstF5I GGATG 5 cut(s) 306, 531, 639, 755, 1377
BstKTI GATC 2 cut(s) 77, 532
BstMAI GTCTC 3 cut(s) 47, 500, 576
BstMBI GATC 2 cut(s) 74, 529
BstMWI GCNNNNNNNGC 2 cut(s) 227, 236
BstNI CCWGG 4 cut(s) 355, 950, 1182, 1356
BstNSI RCATGY 3 cut(s) 450, 850, 934
BstSCI CCNGG 4 cut(s) 353, 948, 1180, 1354
BstSFI CTRYAG 1 cut(s) 669
BstSLI GKGCMC 2 cut(s) 774, 802
BstV1I GCAGC 5 cut(s) 117, 362, 707, 724, 1012
BstV2I GAAGAC 2 cut(s) 614, 650
BstX2I RGATCY 2 cut(s) 74, 529
BstYI RGATCY 2 cut(s) 74, 529
BsuI GTATCC 1 cut(s) 1276
BsuRI GGCC 1 cut(s) 1154
BtsCI GGATG 5 cut(s) 306, 531, 639, 755, 1377
BtsIMutI CAGTG 3 cut(s) 282, 712, 1121
BveI ACCTGC 1 cut(s) 662
Cac8I GCNNGC 5 cut(s) 138, 219, 237, 579, 1230
Csp6I GTAC 4 cut(s) 516, 710, 934, 1199
CviAII CATG 6 cut(s) 226, 447, 594, 847, 931, 1076
CviQI GTAC 4 cut(s) 516, 710, 934, 1199
DdeI CTNAG 2 cut(s) 207, 504
DpnI GATC 2 cut(s) 76, 531
DpnII GATC 2 cut(s) 74, 529
EaeI YGGCCR 1 cut(s) 1152
Eco130I CCWWGG 1 cut(s) 643
EcoRI GAATTC 2 cut(s) 53, 789
EcoRII CCWGG 4 cut(s) 353, 948, 1180, 1354
EcoT14I CCWWGG 1 cut(s) 643
EcoT22I ATGCAT 2 cut(s) 452, 1336
ErhI CCWWGG 1 cut(s) 643
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 6 cut(s) 229, 450, 597, 850, 934, 1079
FatI CATG 6 cut(s) 225, 446, 593, 846, 930, 1075
FauI CCCGC 1 cut(s) 783
FauNDI CATATG 1 cut(s) 452
Fnu4HI GCNGC 8 cut(s) 96, 131, 134, 351, 696, 738, 1026, 1375
FokI GGATG 5 cut(s) 313, 538, 626, 742, 1364
Fsp4HI GCNGC 8 cut(s) 96, 131, 134, 351, 696, 738, 1026, 1375
FspBI CTAG 8 cut(s) 231, 486, 627, 677, 809, 1052, 1142, 1229
GluI GCNGC 8 cut(s) 96, 131, 134, 351, 696, 738, 1026, 1375
GsaI CCCAGC 1 cut(s) 119
HaeIII GGCC 1 cut(s) 1154
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 6 cut(s) 229, 450, 597, 850, 934, 1079
HincII GTYRAC 2 cut(s) 469, 1344
HindII GTYRAC 2 cut(s) 469, 1344
HinfI GANTC 2 cut(s) 5, 395
HpaI GTTAAC 1 cut(s) 1344
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 6 cut(s) 469, 632, 712, 800, 1270, 1344
Hpy188I TCNGA 3 cut(s) 295, 1171, 1382
Hpy188III TCNNGA 4 cut(s) 677, 786, 1081, 1142
Hpy8I GTNNAC 6 cut(s) 469, 632, 712, 800, 1270, 1344
HpyAV CCTTC 1 cut(s) 815
HpyCH4III ACNGT 2 cut(s) 286, 1060
HpyCH4IV ACGT 2 cut(s) 40, 1340
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 236
HpyF3I CTNAG 2 cut(s) 207, 504
HpySE526I ACGT 2 cut(s) 40, 1340
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 6 cut(s) 229, 450, 597, 850, 934, 1079
KspAI GTTAAC 1 cut(s) 1344
Kzo9I GATC 2 cut(s) 74, 529
LmnI GCTCC 1 cut(s) 927
Lsp1109I GCAGC 5 cut(s) 117, 362, 707, 724, 1012
LweI GCATC 6 cut(s) 181, 429, 697, 822, 1304, 1386
MaeI CTAG 8 cut(s) 231, 486, 627, 677, 809, 1052, 1142, 1229
MaeII ACGT 2 cut(s) 40, 1340
MaeIII GTNAC 6 cut(s) 169, 330, 815, 974, 1054, 1117
MalI GATC 2 cut(s) 76, 531
MboI GATC 2 cut(s) 74, 529
MboII GAAGA 9 cut(s) 62, 69, 190, 395, 398, 501, 619, 650, 1217
MflI RGATCY 2 cut(s) 74, 529
MhlI GDGCHC 2 cut(s) 774, 802
MlsI TGGCCA 1 cut(s) 1154
MluNI TGGCCA 1 cut(s) 1154
MnlI CCTC 4 cut(s) 892, 947, 994, 1138
Mox20I TGGCCA 1 cut(s) 1154
Mph1103I ATGCAT 2 cut(s) 452, 1336
MscI TGGCCA 1 cut(s) 1154
MseI TTAA 4 cut(s) 275, 765, 842, 1343
MslI CAYNNNNRTG 1 cut(s) 451
Msp20I TGGCCA 1 cut(s) 1154
MspA1I CMGCKG 1 cut(s) 698
MspR9I CCNGG 4 cut(s) 355, 950, 1182, 1356
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 4 cut(s) 355, 950, 1182, 1356
MwoI GCNNNNNNNGC 2 cut(s) 227, 236
NdeI CATATG 1 cut(s) 452
NdeII GATC 2 cut(s) 74, 529
NheI GCTAGC 1 cut(s) 1228
NlaIII CATG 6 cut(s) 229, 450, 597, 850, 934, 1079
NlaIV GGNNCC 1 cut(s) 264
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 1 cut(s) 169
NsiI ATGCAT 2 cut(s) 452, 1336
NspI RCATGY 3 cut(s) 450, 850, 934
PciI ACATGT 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 580
PctI GAATGC 1 cut(s) 1334
PfeI GAWTC 2 cut(s) 5, 395
PfoI TCCNGGA 1 cut(s) 1354
PkrI GCNGC 8 cut(s) 97, 132, 135, 352, 697, 739, 1027, 1376
PscI ACATGT 1 cut(s) 846
PshBI ATTAAT 1 cut(s) 765
Psp6I CCWGG 4 cut(s) 353, 948, 1180, 1354
PspFI CCCAGC 1 cut(s) 115
PspGI CCWGG 4 cut(s) 353, 948, 1180, 1354
PspN4I GGNNCC 1 cut(s) 264
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 2 cut(s) 74, 529
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 4 cut(s) 517, 711, 935, 1200
RsaNI GTAC 4 cut(s) 516, 710, 934, 1199
RseI CAYNNNNRTG 1 cut(s) 451
SaqAI TTAA 4 cut(s) 275, 765, 842, 1343
SatI GCNGC 8 cut(s) 96, 131, 134, 351, 696, 738, 1026, 1375
Sau3AI GATC 2 cut(s) 74, 529
ScaI AGTACT 1 cut(s) 1200
ScrFI CCNGG 4 cut(s) 355, 950, 1182, 1356
SduI GDGCHC 2 cut(s) 774, 802
SfaNI GCATC 6 cut(s) 181, 429, 697, 822, 1304, 1386
SfcI CTRYAG 1 cut(s) 669
SmiMI CAYNNNNRTG 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SpeI ACTAGT 1 cut(s) 626
SsiI CCGC 6 cut(s) 96, 133, 776, 1133, 1219, 1374
SspI AATATT 1 cut(s) 1236
SspMI CTAG 8 cut(s) 231, 486, 627, 677, 809, 1052, 1142, 1229
StyD4I CCNGG 4 cut(s) 353, 948, 1180, 1354
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 286, 1060
TaiI ACGT 2 cut(s) 43, 1343
TatI WGTACW 2 cut(s) 709, 1198
TauI GCSGC 3 cut(s) 98, 136, 1377
TfiI GAWTC 2 cut(s) 5, 395
Tru1I TTAA 4 cut(s) 275, 765, 842, 1343
Tru9I TTAA 4 cut(s) 275, 765, 842, 1343
TscAI CASTG 3 cut(s) 289, 719, 1128
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 5 cut(s) 130, 350, 695, 737, 1025
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 244, 879, 1339
TspRI CASTG 3 cut(s) 289, 719, 1128
VneI GTGCAC 1 cut(s) 798
VspI ATTAAT 1 cut(s) 765
XapI RAATTY 5 cut(s) 53, 586, 718, 789, 1249
XbaI TCTAGA 2 cut(s) 676, 1141
XceI RCATGY 3 cut(s) 450, 850, 934
XspI CTAG 8 cut(s) 231, 486, 627, 677, 809, 1052, 1142, 1229
ZraI GACGTC 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 1200
Zsp2I ATGCAT 2 cut(s) 452, 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.