MD06G1036200.v1.1

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005057
Physical Location & Seq
Reverse (-)
4656091 .. 4659669
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1036200.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGATTCTCTTCCCAACACCTTCAATCCAAACAAACGTCTCAAAGAAGAGTTTGTGAGCAATTTGAGTGGATCGTCGATGATGGAGATTGCCGCGCTTACCACGATAATCCCTATTCTGATACTTCTCCGGCACTCAGTTGGCTCTCATCGTGCTGTCGATGTTGTATCGAAGAAAAATGATGATGCAGTAGCTGGTTCTAAGGGATGGGCAGCTTACATGGCTTCAATGTCTCTTGATTTTCTTCTCATTATAGTTCCAATCATCTTAGTTTACACTGTTTTGGCAGAGTGGATGTATATCTGGGCAACTTTGTTGATATTGTTACTACTTTTCAGTATTGCAGCCAAAAGGTTTGGTTCCTATTCTACTTTGAAGGAAGATCCCTTTTCTATAAGGCAAACTTTTTCATCTTACAGAGTCGCTACGATGATCATAACATGCGTCTGTATCTTGGCTGTTGACTTCACAATATTCCCTAGAAGATATGCCAAGACTGAGACTTATGGTACTGGCTGGTTGTTGAGCTGCTGGGCAACCACGAGGACGCCGTGCGCCGCCAAAAACGTAGATCTACTGCCACGGCTGCTCTTCCAGTTCCATCCGTTGAGAAACATACCCATAACTTCGAATCCCGAAACCAATGAGGACAAAGAGTTGTTGGAATTGGCCCCGGCTCCTTCCCCCACTGTTTCCTCCTGCTCCTGCGCTGCCACCGCCATATCCGCTGGAAAAGAAAGCTCTGGCTTTTGGGATGAAAAGGAAAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

258

Amino Acids

28.53

Weight (kDa)

7.58

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 12 - 173 1.5e-36 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 4 cut(s) 93, 558, 717, 726
AclWI GGATC 2 cut(s) 79, 377
AcyI GRCGYC 1 cut(s) 548
AfaI GTAC 1 cut(s) 511
AgsI TTSAA 3 cut(s) 25, 228, 376
AluBI AGCT 4 cut(s) 194, 215, 528, 741
AluI AGCT 4 cut(s) 194, 215, 528, 741
Alw26I GTCTC 3 cut(s) 44, 237, 494
AlwI GGATC 2 cut(s) 79, 377
AlwNI CAGNNNCTG 1 cut(s) 194
AoxI GGCC 1 cut(s) 669
ApeKI GCWGC 5 cut(s) 212, 344, 528, 586, 710
AspLEI GCGC 3 cut(s) 97, 557, 710
AspS9I GGNCC 1 cut(s) 670
AsuC2I CCSGG 1 cut(s) 674
AsuII TTCGAA 1 cut(s) 629
BauI CACGAG 1 cut(s) 541
BbvI GCAGC 5 cut(s) 224, 356, 515, 573, 697
BccI CCATC 3 cut(s) 76, 201, 609
BceAI ACGGC 2 cut(s) 535, 599
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 44, 237, 494
BfaI CTAG 1 cut(s) 480
BglII AGATCT 1 cut(s) 571
BisI GCNGC 7 cut(s) 93, 213, 345, 529, 558, 587, 711
BlsI GCNGC 7 cut(s) 94, 214, 346, 530, 559, 588, 712
Bme1390I CCNGG 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 670
BmiI GGNNCC 3 cut(s) 361, 672, 678
BmrFI CCNGG 1 cut(s) 674
BmsI GCATC 1 cut(s) 175
Bpu14I TTCGAA 1 cut(s) 629
BpuMI CCSGG 1 cut(s) 674
BsaBI GATNNNNATC 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 581, 672
Bse1I ACTGG 2 cut(s) 517, 595
Bse8I GATNNNNATC 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 581, 672
BseGI GGATG 4 cut(s) 212, 300, 601, 760
BseJI GATNNNNATC 1 cut(s) 299
BseMII CTCAG 2 cut(s) 150, 489
BseNI ACTGG 2 cut(s) 517, 595
BseXI GCAGC 5 cut(s) 224, 356, 515, 573, 697
BseYI CCCAGC 1 cut(s) 531
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 1 cut(s) 671
BsiSI CCGG 2 cut(s) 130, 674
BsmAI GTCTC 3 cut(s) 44, 237, 494
BsmBI CGTCTC 1 cut(s) 44
BsnI GGCC 1 cut(s) 671
Bsp119I TTCGAA 1 cut(s) 629
Bsp143I GATC 4 cut(s) 71, 382, 432, 571
BspACI CCGC 4 cut(s) 93, 558, 717, 726
BspANI GGCC 1 cut(s) 671
BspCNI CTCAG 2 cut(s) 149, 490
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 3 cut(s) 361, 672, 678
BspPI GGATC 2 cut(s) 79, 377
BspQI GCTCTTC 1 cut(s) 596
BspT104I TTCGAA 1 cut(s) 629
BsrI ACTGG 2 cut(s) 517, 595
BssECI CCNNGG 2 cut(s) 581, 672
BssMI GATC 4 cut(s) 71, 382, 432, 571
BssNI GRCGYC 1 cut(s) 548
BssSI CACGAG 1 cut(s) 541
Bst2BI CACGAG 1 cut(s) 541
Bst4CI ACNGT 2 cut(s) 280, 691
Bst6I CTCTTC 3 cut(s) 15, 42, 596
BstACI GRCGYC 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 629
BstDEI CTNAG 4 cut(s) 136, 201, 268, 498
BstDSI CCRYGG 1 cut(s) 581
BstF5I GGATG 4 cut(s) 212, 300, 601, 760
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 3 cut(s) 97, 557, 710
BstKTI GATC 4 cut(s) 74, 385, 435, 574
BstMAI GTCTC 3 cut(s) 44, 237, 494
BstMBI GATC 4 cut(s) 71, 382, 432, 571
BstMWI GCNNNNNNNGC 4 cut(s) 221, 586, 716, 725
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 672
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 5 cut(s) 224, 356, 515, 573, 697
BstX2I RGATCY 2 cut(s) 382, 571
BstYI RGATCY 2 cut(s) 382, 571
BsuRI GGCC 1 cut(s) 671
BtgI CCRYGG 1 cut(s) 581
BtsCI GGATG 4 cut(s) 212, 300, 601, 760
BtsIMutI CAGTG 2 cut(s) 276, 687
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 3 cut(s) 97, 557, 710
Cfr13I GGNCC 1 cut(s) 670
CseI GACGC 2 cut(s) 433, 556
Csp6I GTAC 1 cut(s) 510
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 2 cut(s) 220, 441
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 4 cut(s) 136, 201, 268, 498
DpnI GATC 4 cut(s) 73, 384, 434, 573
DpnII GATC 4 cut(s) 71, 382, 432, 571
Eam1104I CTCTTC 3 cut(s) 15, 42, 596
EarI CTCTTC 3 cut(s) 15, 42, 596
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 2 cut(s) 223, 444
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FatI CATG 2 cut(s) 219, 440
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 7 cut(s) 93, 213, 345, 529, 558, 587, 711
FokI GGATG 4 cut(s) 219, 307, 588, 767
Fsp4HI GCNGC 7 cut(s) 93, 213, 345, 529, 558, 587, 711
FspBI CTAG 1 cut(s) 480
GlaI GCGC 3 cut(s) 96, 556, 709
GluI GCNGC 7 cut(s) 93, 213, 345, 529, 558, 587, 711
GsaI CCCAGC 1 cut(s) 535
HaeIII GGCC 1 cut(s) 671
HapII CCGG 2 cut(s) 130, 674
HgaI GACGC 2 cut(s) 433, 556
HhaI GCGC 3 cut(s) 97, 557, 710
Hin1I GRCGYC 1 cut(s) 548
Hin1II CATG 2 cut(s) 223, 444
Hin6I GCGC 3 cut(s) 95, 555, 708
HinP1I GCGC 3 cut(s) 95, 555, 708
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 3 cut(s) 5, 420, 631
HpaII CCGG 2 cut(s) 130, 674
Hpy166II GTNNAC 2 cut(s) 274, 463
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 2 cut(s) 236, 635
Hpy8I GTNNAC 2 cut(s) 274, 463
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 31, 370, 690
HpyCH4III ACNGT 2 cut(s) 280, 691
HpyCH4IV ACGT 2 cut(s) 37, 567
HpyCH4V TGCA 2 cut(s) 188, 344
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 586, 716, 725
HpyF3I CTNAG 4 cut(s) 136, 201, 268, 498
HpySE526I ACGT 2 cut(s) 37, 567
Hsp92I GRCGYC 1 cut(s) 548
Hsp92II CATG 2 cut(s) 223, 444
HspAI GCGC 3 cut(s) 95, 555, 708
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 4 cut(s) 71, 382, 432, 571
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 2 cut(s) 682, 707
Lsp1109I GCAGC 5 cut(s) 224, 356, 515, 573, 697
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 480
MaeII ACGT 2 cut(s) 37, 567
MaeIII GTNAC 1 cut(s) 324
MalI GATC 4 cut(s) 73, 384, 434, 573
MboI GATC 4 cut(s) 71, 382, 432, 571
MboII GAAGA 6 cut(s) 59, 184, 236, 392, 495, 583
MflI RGATCY 2 cut(s) 382, 571
MluCI AATT 2 cut(s) 61, 665
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 1 cut(s) 642
MnlI CCTC 3 cut(s) 537, 640, 706
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 2 cut(s) 130, 674
MspR9I CCNGG 1 cut(s) 674
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 4 cut(s) 221, 586, 716, 725
NciI CCSGG 1 cut(s) 674
NdeII GATC 4 cut(s) 71, 382, 432, 571
NlaIII CATG 2 cut(s) 223, 444
NlaIV GGNNCC 3 cut(s) 361, 672, 678
NspI RCATGY 1 cut(s) 444
NspV TTCGAA 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 548
PfeI GAWTC 2 cut(s) 5, 631
PkrI GCNGC 7 cut(s) 94, 214, 346, 530, 559, 588, 712
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 531
PspN4I GGNNCC 3 cut(s) 361, 672, 678
PspPI GGNCC 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 2 cut(s) 382, 571
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 596
SatI GCNGC 7 cut(s) 93, 213, 345, 529, 558, 587, 711
Sau3AI GATC 4 cut(s) 71, 382, 432, 571
Sau96I GGNCC 1 cut(s) 670
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 674
SetI ASST 8 cut(s) 23, 40, 196, 217, 356, 530, 570, 743
SfaNI GCATC 1 cut(s) 175
SfuI TTCGAA 1 cut(s) 629
Sse9I AATT 2 cut(s) 61, 665
SsiI CCGC 4 cut(s) 93, 558, 717, 726
SspI AATATT 1 cut(s) 474
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 672
TaaI ACNGT 2 cut(s) 280, 691
TaiI ACGT 2 cut(s) 40, 570
TaqI TCGA 4 cut(s) 77, 159, 170, 629
TasI AATT 2 cut(s) 61, 665
TauI GCSGC 2 cut(s) 95, 560
TfiI GAWTC 2 cut(s) 5, 631
TscAI CASTG 2 cut(s) 283, 694
TseI GCWGC 5 cut(s) 212, 344, 528, 586, 710
TspDTI ATGAA 2 cut(s) 399, 771
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 2 cut(s) 283, 694
XceI RCATGY 1 cut(s) 444
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.