Rw7G021420

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005057
Physical Location & Seq
Reverse (-)
24881177 .. 24888290
7114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021420.1

Sequence Viewer

Length: 1392 bp
ATGGATTCTGTTCCCAAGTCTTTGAATCCAAACAGGCGTCTCAAGGAAGAATTCGTCAGCAATTTGACTGGATCTTCCATGCTGGAAATCGCCGCCATCACCACAATAATCCCAGCTCTGTTTCTGCTGCGGCGAGCAGTCGGCTTCAATCGTCCTGCTGATGATGTCACATTGAAGAAAAATGATGATGCAAAAGTTGGTTCTAAGAGTTGGCAGGCTTACATGGCTAGGCTGGCTGTGGATTTTCTCTTCATTATGGTTCCATTTGTCTTAATTTTCACTGTCTTATCAGAGTGGATGTATATCTGGGCAACTTTGCTAATTCTGTTACTTGTTTTCAGCATTGCAGCCAGAAGTTCTGGTTTTGGTTCTAATTTGGAAGAACCCGATTCTATTAGGACAAGTATTACATCATTTAGGGTCGCAACGATGATGCTAACATGCATATGTATCTTGGCTGTTGACTTCACAATTTTTCCTAGAAGATATGCCAAGACTGAGACTTATGGTACTGGCTGGATGGATCTCGGGGTTGGCTCATTTGTCGTAGCAAACTCATTTGTGTCTCGGCAAGCACGAAATTTATCATGGACGAAATGGAAGACTGCAATTCAATCTACTAGTCCACTAATCATCCTTGGGTTTGTTCGTCTTCTCTCTACTGCAGGTCTAGACTATCAGCATCATGCAGCTGAATATGGAGTACACTGGAATTTCTTTTTCACACTTGCTGCCATATCATTTCTTACATCCATAATTAATGTGCCCGCGTGGTATTCTGGAATTCTCGGTGCACTAATTCTAGTAGGTTACGAAGGTTGGTTGATGCAGGGGTTAAACACGTATCTGCTTTCTAATGAAAGGGGAAGTGATATAATTAGCCAAAATAAGGAGGGATTTTTTAGCATATTTGGATATTGGAGCATGTACCTCATTGGTGTCCAGGTGGGGAATTTTCTTTTTTTCAGTAACCACTCCTCTGCTACAACAAGAAGCCATAAATGGACAAGGAATAGAGCTGCAATGCTTTCACTTCTGTTTTGGCTAGTAACTGTGGTTATAGATAGCCATGTCCAGAGAGTTTCCCGTAGAATGTGCAATCTGGCTTACGTTACACTGGTATTGGCCGTAAATCTAGAGGGTATGGCCATAGTTCTGTTCTCTGATTGTTTCCCTGGAAGCAAAACATCAGTACTTGAAGAAGCATATAACCGCAATTTGCTAGCAATATTTCTTTTGGCAAATTTGCTTACTGGTCTGGTAAACTTTTTGGTGGATACCCTGTTTGCATCATCAGTCAAAGCTCTTTCTGTGATGATTGCCTATGCATTCACTTTAACTTTCATTCCTGGATTGCTAGACTTTTGCGGCATCCGATTTAGATTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

463

Amino Acids

51.96

Weight (kDa)

9.22

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 12 - 450 9.5e-131 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 656
AccII CGCG 1 cut(s) 770
AciI CCGC 5 cut(s) 93, 130, 768, 1213, 1368
AclWI GGATC 2 cut(s) 79, 531
AcoI YGGCCR 2 cut(s) 1125, 1146
AcsI RAATTY 6 cut(s) 50, 580, 712, 783, 952, 1243
AcyI GRCGYC 1 cut(s) 37
AfaI GTAC 4 cut(s) 511, 705, 929, 1194
AfiI CCNNNNNNNGG 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 5 cut(s) 25, 148, 175, 614, 1199
AhlI ACTAGT 1 cut(s) 620
AjnI CCWGG 3 cut(s) 942, 1174, 1348
AluBI AGCT 4 cut(s) 116, 692, 1019, 1304
AluI AGCT 4 cut(s) 116, 692, 1019, 1304
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 3 cut(s) 44, 494, 570
Alw44I GTGCAC 1 cut(s) 792
AlwI GGATC 2 cut(s) 79, 531
Ama87I CYCGRG 1 cut(s) 527
AoxI GGCC 2 cut(s) 1125, 1146
ApaLI GTGCAC 1 cut(s) 792
ApeKI GCWGC 5 cut(s) 127, 347, 689, 731, 1019
ApoI RAATTY 6 cut(s) 50, 580, 712, 783, 952, 1243
AseI ATTAAT 1 cut(s) 759
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 1222
AvaI CYCGRG 1 cut(s) 527
BaeGI GKGCMC 2 cut(s) 768, 796
BalI TGGCCA 1 cut(s) 1148
BbsI GAAGAC 2 cut(s) 608, 644
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 5 cut(s) 114, 359, 701, 718, 1006
BccI CCATC 2 cut(s) 104, 514
BceAI ACGGC 1 cut(s) 1112
BciT130I CCWGG 3 cut(s) 944, 1176, 1350
BciVI GTATCC 1 cut(s) 1270
BcoDI GTCTC 3 cut(s) 44, 494, 570
BcuI ACTAGT 1 cut(s) 620
BfaI CTAG 9 cut(s) 228, 480, 621, 671, 803, 1046, 1136, 1223, 1358
BfmI CTRYAG 1 cut(s) 663
BfuAI ACCTGC 1 cut(s) 656
BfuI GTATCC 1 cut(s) 1270
BisI GCNGC 8 cut(s) 93, 128, 131, 348, 690, 732, 1020, 1369
BlsI GCNGC 8 cut(s) 94, 129, 132, 349, 691, 733, 1021, 1370
BmcAI AGTACT 1 cut(s) 1194
Bme1390I CCNGG 3 cut(s) 944, 1176, 1350
BmeT110I CYCGRG 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 3 cut(s) 944, 1176, 1350
BmsI GCATC 6 cut(s) 178, 423, 691, 816, 1298, 1380
BmtI GCTAGC 1 cut(s) 1226
BpiI GAAGAC 2 cut(s) 608, 644
BpuEI CTTGAG 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 843
BsaBI GATNNNNATC 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 637, 1174
Bsc4I CCNNNNNNNGG 1 cut(s) 889
Bse1I ACTGG 5 cut(s) 73, 517, 713, 1122, 1258
Bse3DI GCAATG 2 cut(s) 342, 1029
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 3 cut(s) 944, 1176, 1350
BseDI CCNNGG 2 cut(s) 637, 1174
BseGI GGATG 5 cut(s) 303, 525, 633, 749, 1371
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 889
BseMI GCAATG 2 cut(s) 342, 1029
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 5 cut(s) 73, 517, 713, 1122, 1258
BseRI GAGGAG 1 cut(s) 967
BseSI GKGCMC 2 cut(s) 768, 796
BseXI GCAGC 5 cut(s) 114, 359, 701, 718, 1006
BseYI CCCAGC 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 770
BshFI GGCC 2 cut(s) 1127, 1148
BsiHKAI GWGCWC 1 cut(s) 796
BsiHKCI CYCGRG 1 cut(s) 527
BslI CCNNNNNNNGG 1 cut(s) 889
BsmAI GTCTC 3 cut(s) 44, 494, 570
BsmBI CGTCTC 1 cut(s) 44
BsmI GAATGC 1 cut(s) 1328
BsnI GGCC 2 cut(s) 1127, 1148
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 768, 796
Bsp143I GATC 2 cut(s) 71, 523
BspACI CCGC 5 cut(s) 93, 130, 768, 1213, 1368
BspANI GGCC 2 cut(s) 1127, 1148
BspCNI CTCAG 1 cut(s) 490
BspFNI CGCG 1 cut(s) 770
BspLI GGNNCC 1 cut(s) 261
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 1 cut(s) 656
BspOI GCTAGC 1 cut(s) 1226
BspPI GGATC 2 cut(s) 79, 531
BsrDI GCAATG 2 cut(s) 342, 1029
BsrI ACTGG 5 cut(s) 73, 517, 713, 1122, 1258
BssECI CCNNGG 2 cut(s) 637, 1174
BssMI GATC 2 cut(s) 71, 523
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 637
Bst2UI CCWGG 3 cut(s) 944, 1176, 1350
Bst4CI ACNGT 2 cut(s) 283, 1054
Bst6I CTCTTC 1 cut(s) 254
BstACI GRCGYC 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 843
BstC8I GCNNGC 6 cut(s) 135, 216, 234, 573, 768, 1224
BstDEI CTNAG 2 cut(s) 204, 498
BstF5I GGATG 5 cut(s) 303, 525, 633, 749, 1371
BstFNI CGCG 1 cut(s) 770
BstKTI GATC 2 cut(s) 74, 526
BstMAI GTCTC 3 cut(s) 44, 494, 570
BstMBI GATC 2 cut(s) 71, 523
BstMWI GCNNNNNNNGC 2 cut(s) 224, 233
BstNI CCWGG 3 cut(s) 944, 1176, 1350
BstNSI RCATGY 2 cut(s) 444, 928
BstSCI CCNGG 3 cut(s) 942, 1174, 1348
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 2 cut(s) 768, 796
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 5 cut(s) 114, 359, 701, 718, 1006
BstV2I GAAGAC 2 cut(s) 608, 644
BstX2I RGATCY 2 cut(s) 71, 523
BstYI RGATCY 2 cut(s) 71, 523
BsuI GTATCC 1 cut(s) 1270
BsuRI GGCC 2 cut(s) 1127, 1148
BtsCI GGATG 5 cut(s) 303, 525, 633, 749, 1371
BtsIMutI CAGTG 3 cut(s) 279, 706, 1115
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 6 cut(s) 135, 216, 234, 573, 768, 1224
CseI GACGC 1 cut(s) 26
Csp6I GTAC 4 cut(s) 510, 704, 928, 1193
CviAII CATG 7 cut(s) 79, 223, 441, 588, 686, 925, 1070
CviQI GTAC 4 cut(s) 510, 704, 928, 1193
DdeI CTNAG 2 cut(s) 204, 498
DpnI GATC 2 cut(s) 73, 525
DpnII GATC 2 cut(s) 71, 523
EaeI YGGCCR 2 cut(s) 1125, 1146
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 637
Eco88I CYCGRG 1 cut(s) 527
EcoRI GAATTC 2 cut(s) 50, 783
EcoRII CCWGG 3 cut(s) 942, 1174, 1348
EcoT14I CCWWGG 1 cut(s) 637
EcoT22I ATGCAT 2 cut(s) 446, 1330
ErhI CCWWGG 1 cut(s) 637
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 7 cut(s) 82, 226, 444, 591, 689, 928, 1073
FatI CATG 7 cut(s) 78, 222, 440, 587, 685, 924, 1069
FauI CCCGC 1 cut(s) 775
FauNDI CATATG 1 cut(s) 446
Fnu4HI GCNGC 8 cut(s) 93, 128, 131, 348, 690, 732, 1020, 1369
FokI GGATG 5 cut(s) 310, 532, 620, 736, 1358
Fsp4HI GCNGC 8 cut(s) 93, 128, 131, 348, 690, 732, 1020, 1369
FspBI CTAG 9 cut(s) 228, 480, 621, 671, 803, 1046, 1136, 1223, 1358
GluI GCNGC 8 cut(s) 93, 128, 131, 348, 690, 732, 1020, 1369
GsaI CCCAGC 1 cut(s) 116
HaeIII GGCC 2 cut(s) 1127, 1148
HgaI GACGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 7 cut(s) 82, 226, 444, 591, 689, 928, 1073
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 3 cut(s) 5, 25, 389
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 5 cut(s) 463, 626, 706, 794, 1264
Hpy188I TCNGA 3 cut(s) 292, 1165, 1376
Hpy188III TCNNGA 4 cut(s) 671, 780, 1075, 1136
Hpy8I GTNNAC 5 cut(s) 463, 626, 706, 794, 1264
HpyAV CCTTC 1 cut(s) 809
HpyCH4III ACNGT 2 cut(s) 283, 1054
HpyCH4IV ACGT 2 cut(s) 842, 1110
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 233
HpyF3I CTNAG 2 cut(s) 204, 498
HpySE526I ACGT 2 cut(s) 842, 1110
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 7 cut(s) 82, 226, 444, 591, 689, 928, 1073
Kzo9I GATC 2 cut(s) 71, 523
LmnI GCTCC 1 cut(s) 921
Lsp1109I GCAGC 5 cut(s) 114, 359, 701, 718, 1006
LweI GCATC 6 cut(s) 178, 423, 691, 816, 1298, 1380
MaeI CTAG 9 cut(s) 228, 480, 621, 671, 803, 1046, 1136, 1223, 1358
MaeII ACGT 2 cut(s) 842, 1110
MaeIII GTNAC 6 cut(s) 166, 327, 809, 968, 1048, 1111
MalI GATC 2 cut(s) 73, 525
MboI GATC 2 cut(s) 71, 523
MboII GAAGA 9 cut(s) 59, 66, 187, 241, 392, 495, 613, 644, 1211
MflI RGATCY 2 cut(s) 71, 523
MhlI GDGCHC 2 cut(s) 768, 796
MlsI TGGCCA 1 cut(s) 1148
MluNI TGGCCA 1 cut(s) 1148
MnlI CCTC 4 cut(s) 886, 941, 988, 1132
Mox20I TGGCCA 1 cut(s) 1148
Mph1103I ATGCAT 2 cut(s) 446, 1330
MscI TGGCCA 1 cut(s) 1148
MseI TTAA 4 cut(s) 272, 759, 836, 1337
MslI CAYNNNNRTG 1 cut(s) 445
Msp20I TGGCCA 1 cut(s) 1148
MspA1I CMGCKG 1 cut(s) 692
MspR9I CCNGG 3 cut(s) 944, 1176, 1350
Mva1269I GAATGC 1 cut(s) 1328
MvaI CCWGG 3 cut(s) 944, 1176, 1350
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 2 cut(s) 224, 233
NdeI CATATG 1 cut(s) 446
NdeII GATC 2 cut(s) 71, 523
NheI GCTAGC 1 cut(s) 1222
NlaIII CATG 7 cut(s) 82, 226, 444, 591, 689, 928, 1073
NlaIV GGNNCC 1 cut(s) 261
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 2 cut(s) 446, 1330
NspI RCATGY 2 cut(s) 444, 928
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 1 cut(s) 1328
PfeI GAWTC 3 cut(s) 5, 25, 389
PfoI TCCNGGA 1 cut(s) 1348
PkrI GCNGC 8 cut(s) 94, 129, 132, 349, 691, 733, 1021, 1370
Ppu21I YACGTR 1 cut(s) 843
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 3 cut(s) 942, 1174, 1348
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 3 cut(s) 942, 1174, 1348
PspN4I GGNNCC 1 cut(s) 261
PstI CTGCAG 1 cut(s) 667
PsuI RGATCY 2 cut(s) 71, 523
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 4 cut(s) 511, 705, 929, 1194
RsaNI GTAC 4 cut(s) 510, 704, 928, 1193
RseI CAYNNNNRTG 1 cut(s) 445
SaqAI TTAA 4 cut(s) 272, 759, 836, 1337
SatI GCNGC 8 cut(s) 93, 128, 131, 348, 690, 732, 1020, 1369
Sau3AI GATC 2 cut(s) 71, 523
ScaI AGTACT 1 cut(s) 1194
ScrFI CCNGG 3 cut(s) 944, 1176, 1350
SduI GDGCHC 2 cut(s) 768, 796
SfaNI GCATC 6 cut(s) 178, 423, 691, 816, 1298, 1380
SfcI CTRYAG 1 cut(s) 663
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 620
SsiI CCGC 5 cut(s) 93, 130, 768, 1213, 1368
SspI AATATT 1 cut(s) 1230
SspMI CTAG 9 cut(s) 228, 480, 621, 671, 803, 1046, 1136, 1223, 1358
StyD4I CCNGG 3 cut(s) 942, 1174, 1348
StyI CCWWGG 1 cut(s) 637
TaaI ACNGT 2 cut(s) 283, 1054
TaiI ACGT 2 cut(s) 845, 1113
TatI WGTACW 2 cut(s) 703, 1192
TauI GCSGC 3 cut(s) 95, 133, 1371
TfiI GAWTC 3 cut(s) 5, 25, 389
Tru1I TTAA 4 cut(s) 272, 759, 836, 1337
Tru9I TTAA 4 cut(s) 272, 759, 836, 1337
TscAI CASTG 3 cut(s) 286, 713, 1122
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 5 cut(s) 127, 347, 689, 731, 1019
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 3 cut(s) 241, 873, 1333
TspRI CASTG 3 cut(s) 286, 713, 1122
VneI GTGCAC 1 cut(s) 792
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 6 cut(s) 50, 580, 712, 783, 952, 1243
XbaI TCTAGA 2 cut(s) 670, 1135
XceI RCATGY 2 cut(s) 444, 928
XspI CTAG 9 cut(s) 228, 480, 621, 671, 803, 1046, 1136, 1223, 1358
ZrmI AGTACT 1 cut(s) 1194
Zsp2I ATGCAT 2 cut(s) 446, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.