Rorug07G0118600

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005057
Physical Location & Seq
Forward (+)
9317577 .. 9319138
1562 bp
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UTR
Exon/CDS
Intron
Rorug07G0118600.1

Sequence Viewer

Length: 660 bp
ATGAAGCCCCACCGCACCCTGAGGCCTCCGACATCAGAAACTTCCACTCCGACGACTACGTCGACTTCCTCGCCTCCGTCTCCCCCGAAACCCTCTCCGACGCCGTCCACGCCCGCCACCTCAAGCGCTTCAACATCGGCGAGGACTGCCCCCTTCTTCGACGGCTTCTTCCAGGCCTCCTCCGGCGGCTCTATTGGCGCCGCCGTCAAGCTGAACCGGAGGGACTGCGATATTGCGATCAATTGCGCCGGTGGAGTTCACCATGCCAAGAAAGCTGAGGCCTCAGGGTTTTGGTTAATTTTTCTGTGTGGAGTAGTTAGTTTAGTGCTTATGGATCTGGTAATTCTTGCTGCACAGCGTGTTCTTTATGTAGACATTGATGTTCACCACGGAGACGGAGTTGAGGAGGCATTTTACACTACTGACAGGATGCAGTTGTTTTTCAGTGTGGAGCGGATTCACTATCTGGTGATCGGTTGGGGTGCTTCAACTTTATCTGTCAAAGGTCATGCCGTTTGTCGCCGCTATCTTCAATCTTTCAATGTTCCTTTGATGGTTTTCGGCGGCGGAGGATATACGATCAGGAATGTTGCTCGTTGCTGGTGCTATGAGATAGCAGTTGCTGTTGGGGTGGATCCTTACAATAAATACTACGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

219

Amino Acids

23.71

Weight (kDa)

8.53

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hist_deacetyl PF00850 57 - 146 8e-19 Histone deacetylase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 58
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 454
AccI GTMKAC 2 cut(s) 62, 372
AciI CCGC 8 cut(s) 13, 114, 186, 201, 454, 523, 564, 567
AclWI GGATC 3 cut(s) 342, 629, 642
AcyI GRCGYC 2 cut(s) 101, 198
AdeI CACNNNGTG 1 cut(s) 359
AfeI AGCGCT 1 cut(s) 127
AgsI TTSAA 4 cut(s) 132, 489, 533, 541
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 2 cut(s) 211, 275
AluI AGCT 2 cut(s) 211, 275
Alw26I GTCTC 2 cut(s) 84, 387
AlwI GGATC 3 cut(s) 342, 629, 642
AlwNI CAGNNNCTG 1 cut(s) 623
Aor51HI AGCGCT 1 cut(s) 127
AoxI GGCC 3 cut(s) 23, 174, 279
ApeKI GCWGC 1 cut(s) 350
AspLEI GCGC 3 cut(s) 128, 200, 248
AsuHPI GGTGA 3 cut(s) 251, 377, 481
AxyI CCTNAGG 2 cut(s) 20, 283
BamHI GGATCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 197
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 337
BccI CCATC 1 cut(s) 547
BceAI ACGGC 4 cut(s) 88, 178, 188, 497
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 84, 387
BfoI RGCGCY 2 cut(s) 129, 201
BisI GCNGC 5 cut(s) 187, 201, 351, 523, 565
BlsI GCNGC 5 cut(s) 188, 202, 352, 524, 566
Bme1390I CCNGG 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 199, 636
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 106
BsaHI GRCGYC 2 cut(s) 101, 198
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 384, 414
Bse118I RCCGGY 1 cut(s) 248
Bse21I CCTNAGG 2 cut(s) 20, 283
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 435
BseMII CTCAG 3 cut(s) 11, 267, 297
BseRI GAGGAG 2 cut(s) 169, 419
BseXI GCAGC 1 cut(s) 337
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 3 cut(s) 25, 176, 281
BshNI GGYRCC 1 cut(s) 197
BsiSI CCGG 3 cut(s) 183, 217, 249
BslFI GGGAC 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 84, 387
BsmBI CGTCTC 2 cut(s) 84, 387
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 3 cut(s) 25, 176, 281
Bsp143I GATC 5 cut(s) 237, 334, 471, 579, 634
BspACI CCGC 8 cut(s) 13, 114, 186, 201, 454, 523, 564, 567
BspANI GGCC 3 cut(s) 25, 176, 281
BspCNI CTCAG 3 cut(s) 12, 268, 296
BspLI GGNNCC 2 cut(s) 199, 636
BspPI GGATC 3 cut(s) 342, 629, 642
BspT107I GGYRCC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 5 cut(s) 237, 334, 471, 579, 634
BssNI GRCGYC 2 cut(s) 101, 198
Bst2UI CCWGG 1 cut(s) 173
BstACI GRCGYC 2 cut(s) 101, 198
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 3 cut(s) 20, 276, 283
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 1 cut(s) 435
BstH2I RGCGCY 2 cut(s) 129, 201
BstHHI GCGC 3 cut(s) 128, 200, 248
BstKTI GATC 5 cut(s) 240, 337, 474, 582, 637
BstMAI GTCTC 2 cut(s) 84, 387
BstMBI GATC 5 cut(s) 237, 334, 471, 579, 634
BstMWI GCNNNNNNNGC 4 cut(s) 109, 146, 195, 272
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 337
BstX2I RGATCY 2 cut(s) 334, 634
BstYI RGATCY 2 cut(s) 334, 634
Bsu36I CCTNAGG 2 cut(s) 20, 283
BsuRI GGCC 3 cut(s) 25, 176, 281
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 451
Cac8I GCNNGC 1 cut(s) 114
CaiI CAGNNNCTG 1 cut(s) 623
CfoI GCGC 3 cut(s) 128, 200, 248
Cfr10I RCCGGY 1 cut(s) 248
CseI GACGC 1 cut(s) 109
CviAII CATG 2 cut(s) 263, 509
CviJI RGCY 8 cut(s) 7, 25, 165, 176, 189, 211, 275, 281
CviKI_1 RGCY 8 cut(s) 7, 25, 165, 176, 189, 211, 275, 281
DdeI CTNAG 3 cut(s) 20, 276, 283
DinI GGCGCC 1 cut(s) 199
DpnI GATC 5 cut(s) 239, 336, 473, 581, 636
DpnII GATC 5 cut(s) 237, 334, 471, 579, 634
DraIII CACNNNGTG 1 cut(s) 359
DrdI GACNNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 582
Eco147I AGGCCT 3 cut(s) 25, 176, 281
Eco47III AGCGCT 1 cut(s) 127
Eco81I CCTNAGG 2 cut(s) 20, 283
EcoRII CCWGG 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
Esp3I CGTCTC 2 cut(s) 84, 387
FaeI CATG 2 cut(s) 266, 512
FaiI YATR 6 cut(s) 264, 332, 369, 510, 576, 609
FaqI GGGAC 1 cut(s) 236
FatI CATG 2 cut(s) 262, 508
FauI CCCGC 1 cut(s) 121
FblI GTMKAC 2 cut(s) 62, 372
Fnu4HI GCNGC 5 cut(s) 187, 201, 351, 523, 565
FokI GGATG 1 cut(s) 442
Fsp4HI GCNGC 5 cut(s) 187, 201, 351, 523, 565
GlaI GCGC 3 cut(s) 127, 199, 247
GluI GCNGC 5 cut(s) 187, 201, 351, 523, 565
HaeII RGCGCY 2 cut(s) 129, 201
HaeIII GGCC 3 cut(s) 25, 176, 281
HapII CCGG 3 cut(s) 183, 217, 249
HgaI GACGC 1 cut(s) 109
HhaI GCGC 3 cut(s) 128, 200, 248
Hin1I GRCGYC 2 cut(s) 101, 198
Hin1II CATG 2 cut(s) 266, 512
Hin6I GCGC 3 cut(s) 126, 198, 246
HinP1I GCGC 3 cut(s) 126, 198, 246
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 1 cut(s) 457
HpaII CCGG 3 cut(s) 183, 217, 249
HphI GGTGA 3 cut(s) 251, 377, 481
Hpy166II GTNNAC 5 cut(s) 63, 108, 259, 373, 385
Hpy188I TCNGA 4 cut(s) 30, 37, 51, 99
Hpy188III TCNNGA 1 cut(s) 583
Hpy8I GTNNAC 5 cut(s) 63, 108, 259, 373, 385
Hpy99I CGWCG 4 cut(s) 55, 64, 103, 164
HpyAV CCTTC 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 353, 433
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 146, 195, 272
HpyF3I CTNAG 3 cut(s) 20, 276, 283
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 101, 198
Hsp92II CATG 2 cut(s) 266, 512
HspAI GCGC 3 cut(s) 126, 198, 246
KasI GGCGCC 1 cut(s) 197
Kzo9I GATC 5 cut(s) 237, 334, 471, 579, 634
LmnI GCTCC 1 cut(s) 451
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 1 cut(s) 420
MaeII ACGT 1 cut(s) 59
MalI GATC 5 cut(s) 239, 336, 473, 581, 636
MbiI CCGCTC 1 cut(s) 454
MboI GATC 5 cut(s) 237, 334, 471, 579, 634
MboII GAAGA 3 cut(s) 148, 160, 521
MfeI CAATTG 1 cut(s) 241
MflI RGATCY 2 cut(s) 334, 634
MluCI AATT 3 cut(s) 241, 297, 342
Mly113I GGCGCC 1 cut(s) 198
MmeI TCCRAC 3 cut(s) 53, 74, 122
MseI TTAA 1 cut(s) 296
MspI CCGG 3 cut(s) 183, 217, 249
MspR9I CCNGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 241
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 4 cut(s) 109, 146, 195, 272
NarI GGCGCC 1 cut(s) 198
NdeII GATC 5 cut(s) 237, 334, 471, 579, 634
NlaIII CATG 2 cut(s) 266, 512
NlaIV GGNNCC 2 cut(s) 199, 636
PceI AGGCCT 3 cut(s) 25, 176, 281
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PfeI GAWTC 1 cut(s) 457
PflFI GACNNNGTC 2 cut(s) 58, 103
PkrI GCNGC 5 cut(s) 188, 202, 352, 524, 566
PluTI GGCGCC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 2 cut(s) 199, 636
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 2 cut(s) 334, 634
PsyI GACNNNGTC 2 cut(s) 58, 103
SalI GTCGAC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 5 cut(s) 187, 201, 351, 523, 565
Sau3AI GATC 5 cut(s) 237, 334, 471, 579, 634
ScrFI CCNGG 1 cut(s) 173
SetI ASST 5 cut(s) 62, 122, 213, 277, 508
SfaNI GCATC 1 cut(s) 420
SfoI GGCGCC 1 cut(s) 199
SgrAI CRCCGGYG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 3 cut(s) 241, 297, 342
SseBI AGGCCT 3 cut(s) 25, 176, 281
SsiI CCGC 8 cut(s) 13, 114, 186, 201, 454, 523, 564, 567
SspDI GGCGCC 1 cut(s) 197
StuI AGGCCT 3 cut(s) 25, 176, 281
StyD4I CCNGG 1 cut(s) 171
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 62, 159
TasI AATT 3 cut(s) 241, 297, 342
TauI GCSGC 4 cut(s) 189, 203, 525, 567
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TscAI CASTG 1 cut(s) 451
TseI GCWGC 1 cut(s) 350
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 66, 405, 411
TspRI CASTG 1 cut(s) 451
Tth111I GACNNNGTC 2 cut(s) 58, 103
XmiI GTMKAC 2 cut(s) 62, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.