Rroxscaffold_3G00248610

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005057
Physical Location & Seq
Forward (+)
41966843 .. 41974833
7991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248610.1

Sequence Viewer

Length: 1395 bp
ATGGATTCTGTTCCCAATTCTTTCAATCCAAACAGACGTCTCAAGGAAGAATTCGTCAGCAATTTGACTGGATCTTCCATGCTGGAAATCGCTGCCATCACCACAATAATCCCAGCTCTGTTTCTGCTGCGGCGAGCAGTCGGCTTCAATCGTCCTGCTGATGATGTCACATTGAAGAAAAATGATGATGCAAAAGTTGGTTCTAAGAGTTGGCAGGCTTACATGGCTAGGCTGGCTGTGGATTTTCTATTCATTATGGTTCCATTCGTCTTAATTTTCACTGTCTTATCAGAGTGGATGTATATCTGGGCAACTTTGCTAATTCTGTTACTTGTTTTCAGCTTTGCAGCCAGAAGTTTCGGTTTTGGTTCTACTTTGGAAGAAGAACCCGATTCTATTAGGACAAGTATTACATCATTTAGGGTTGCAACGATGATGCTAACATGCATATGCATCTTGGCTGTTGACTTCACAATTTTTCCTAGAAGATATGCCAAGACTGAGACTTATGGTACTGGCTGGATGGATCTCGGGGTTGGCTCATTTGTCGTAGCAAACTCATTTGTGTCTCGGCAAGCACGAAATTTATCATGGACGAAATGGAAGACTGCAATTCAATCTACTAGTCCACTAATCATCCTTGGGTTTGTTCGTCTTCTCTCTACTACAGGTCTAGACTATCAGCATCATGCAGCTGAATATGGAGTACACTGGAATTTCTTTTTCACACTTGCTGCCATATCATTTCTTACATCCATAATTAATGTGCCCGCGCGGTATTCTGGAATTCTCGGTGCACTAATTCTAGTAGGTTATGAAGGTTGGTTGATGCAGGGTTTAAACACATATCTGCTTTCTAATGAAAGGGGAAGTGATATAATTAGCCAAAATAAGGAGGGATTTTTTAGCATATTTGGATATTGGAGCATGTACCTCATTGGTGTCCAGGTGGGGAATTTTCTTTTTTTCAGTAACCACTCCTCTGCTACAACAAGAAGCCATAAATGGACAAGGAATAGAGCTGCAATACTTTCACTTCTGTTTTGGCTAGTAACTGTGGTTATAGATAGCCATGTCCAGAGAGTTTCCCGTAGAATGTGCAATCTGGCTTATGTTACACTGGTATTGGCGGTAAATCTAGAGGGTATGGCTATAGTTCTGTTCTCTGATTGTTTCCCTGGAAGCAAAACATCAGTACTTGAAGAAGCATATAACCGCAATTTGCTAGCAATATTTCTTTTGGCAAATTTGCTTACTGGTCTGGTAAACTTTTTGGTGGATACCCTGTTTGCATCATCAGTCAAAGCTCTTTCTGTGATGATTGCCTATGCATTCACGTTAACTTTCATTCCTGGATTGCTAGACTTTTGCGGCATCCGATTTAGATTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

464

Amino Acids

52.17

Weight (kDa)

9.13

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 12 - 451 6.6e-131 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 40
AccII CGCG 2 cut(s) 773, 775
AciI CCGC 6 cut(s) 130, 771, 775, 1130, 1216, 1371
AclWI GGATC 2 cut(s) 79, 534
AcsI RAATTY 6 cut(s) 50, 583, 715, 786, 955, 1246
AcyI GRCGYC 1 cut(s) 37
AfaI GTAC 4 cut(s) 514, 708, 932, 1197
AfiI CCNNNNNNNGG 1 cut(s) 892
AgsI TTSAA 5 cut(s) 25, 148, 175, 617, 1202
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 3 cut(s) 945, 1177, 1351
AluBI AGCT 5 cut(s) 116, 342, 695, 1022, 1307
AluI AGCT 5 cut(s) 116, 342, 695, 1022, 1307
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 3 cut(s) 44, 497, 573
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 2 cut(s) 79, 534
Ama87I CYCGRG 1 cut(s) 530
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 6 cut(s) 92, 127, 347, 692, 734, 1022
ApoI RAATTY 6 cut(s) 50, 583, 715, 786, 955, 1246
AseI ATTAAT 1 cut(s) 762
AspLEI GCGC 1 cut(s) 775
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 1225
AvaI CYCGRG 1 cut(s) 530
BaeGI GKGCMC 2 cut(s) 771, 799
BbsI GAAGAC 2 cut(s) 611, 647
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 6 cut(s) 79, 114, 359, 704, 721, 1009
BccI CCATC 2 cut(s) 104, 517
BciT130I CCWGG 3 cut(s) 947, 1179, 1353
BciVI GTATCC 1 cut(s) 1273
BcoDI GTCTC 3 cut(s) 44, 497, 573
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1049, 1139, 1226, 1361
BfmI CTRYAG 2 cut(s) 666, 1152
BfuI GTATCC 1 cut(s) 1273
BisI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1023, 1372
BlsI GCNGC 8 cut(s) 94, 129, 132, 349, 694, 736, 1024, 1373
BmcAI AGTACT 1 cut(s) 1197
Bme1390I CCNGG 3 cut(s) 947, 1179, 1353
BmeT110I CYCGRG 1 cut(s) 530
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 3 cut(s) 947, 1179, 1353
BmsI GCATC 7 cut(s) 178, 426, 462, 694, 819, 1301, 1383
BmtI GCTAGC 1 cut(s) 1229
BpiI GAAGAC 2 cut(s) 611, 647
BpuEI CTTGAG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 640, 1177
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse1I ACTGG 5 cut(s) 73, 520, 716, 1125, 1261
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 3 cut(s) 947, 1179, 1353
BseDI CCNNGG 2 cut(s) 640, 1177
BseGI GGATG 5 cut(s) 303, 528, 636, 752, 1374
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 5 cut(s) 73, 520, 716, 1125, 1261
BseRI GAGGAG 1 cut(s) 970
BseSI GKGCMC 2 cut(s) 771, 799
BseXI GCAGC 6 cut(s) 79, 114, 359, 704, 721, 1009
BseYI CCCAGC 1 cut(s) 112
Bsh1236I CGCG 2 cut(s) 773, 775
BsiHKAI GWGCWC 1 cut(s) 799
BsiHKCI CYCGRG 1 cut(s) 530
BslI CCNNNNNNNGG 1 cut(s) 892
BsmAI GTCTC 3 cut(s) 44, 497, 573
BsmBI CGTCTC 1 cut(s) 44
BsmI GAATGC 1 cut(s) 1331
BsoBI CYCGRG 1 cut(s) 530
Bsp1286I GDGCHC 2 cut(s) 771, 799
Bsp143I GATC 2 cut(s) 71, 526
BspACI CCGC 6 cut(s) 130, 771, 775, 1130, 1216, 1371
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 2 cut(s) 773, 775
BspLI GGNNCC 1 cut(s) 261
BspOI GCTAGC 1 cut(s) 1229
BspPI GGATC 2 cut(s) 79, 534
BsrI ACTGG 5 cut(s) 73, 520, 716, 1125, 1261
BssECI CCNNGG 2 cut(s) 640, 1177
BssMI GATC 2 cut(s) 71, 526
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 3 cut(s) 947, 1179, 1353
Bst4CI ACNGT 2 cut(s) 283, 1057
BstACI GRCGYC 1 cut(s) 37
BstC8I GCNNGC 6 cut(s) 135, 216, 234, 576, 771, 1227
BstDEI CTNAG 2 cut(s) 204, 501
BstF5I GGATG 5 cut(s) 303, 528, 636, 752, 1374
BstFNI CGCG 2 cut(s) 773, 775
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 2 cut(s) 74, 529
BstMAI GTCTC 3 cut(s) 44, 497, 573
BstMBI GATC 2 cut(s) 71, 526
BstMWI GCNNNNNNNGC 2 cut(s) 224, 233
BstNI CCWGG 3 cut(s) 947, 1179, 1353
BstNSI RCATGY 2 cut(s) 447, 931
BstSCI CCNGG 3 cut(s) 945, 1177, 1351
BstSFI CTRYAG 2 cut(s) 666, 1152
BstSLI GKGCMC 2 cut(s) 771, 799
BstUI CGCG 2 cut(s) 773, 775
BstV1I GCAGC 6 cut(s) 79, 114, 359, 704, 721, 1009
BstV2I GAAGAC 2 cut(s) 611, 647
BstX2I RGATCY 2 cut(s) 71, 526
BstYI RGATCY 2 cut(s) 71, 526
BsuI GTATCC 1 cut(s) 1273
BtsCI GGATG 5 cut(s) 303, 528, 636, 752, 1374
BtsIMutI CAGTG 3 cut(s) 279, 709, 1118
Cac8I GCNNGC 6 cut(s) 135, 216, 234, 576, 771, 1227
CfoI GCGC 1 cut(s) 775
Csp6I GTAC 4 cut(s) 513, 707, 931, 1196
CviAII CATG 7 cut(s) 79, 223, 444, 591, 689, 928, 1073
CviQI GTAC 4 cut(s) 513, 707, 931, 1196
DdeI CTNAG 2 cut(s) 204, 501
DpnI GATC 2 cut(s) 73, 528
DpnII GATC 2 cut(s) 71, 526
DraI TTTAAA 1 cut(s) 840
Eco130I CCWWGG 1 cut(s) 640
Eco88I CYCGRG 1 cut(s) 530
EcoRI GAATTC 2 cut(s) 50, 786
EcoRII CCWGG 3 cut(s) 945, 1177, 1351
EcoT14I CCWWGG 1 cut(s) 640
EcoT22I ATGCAT 3 cut(s) 449, 455, 1333
ErhI CCWWGG 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1076
FatI CATG 7 cut(s) 78, 222, 443, 590, 688, 927, 1072
FauI CCCGC 1 cut(s) 778
FauNDI CATATG 1 cut(s) 449
Fnu4HI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1023, 1372
FokI GGATG 5 cut(s) 310, 535, 623, 739, 1361
Fsp4HI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1023, 1372
FspBI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1049, 1139, 1226, 1361
GlaI GCGC 1 cut(s) 774
GluI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1023, 1372
GsaI CCCAGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 775
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1076
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HincII GTYRAC 2 cut(s) 466, 1341
HindII GTYRAC 2 cut(s) 466, 1341
HinfI GANTC 2 cut(s) 5, 392
HpaI GTTAAC 1 cut(s) 1341
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 6 cut(s) 466, 629, 709, 797, 1267, 1341
Hpy188I TCNGA 3 cut(s) 292, 1168, 1379
Hpy188III TCNNGA 4 cut(s) 674, 783, 1078, 1139
Hpy8I GTNNAC 6 cut(s) 466, 629, 709, 797, 1267, 1341
HpyAV CCTTC 1 cut(s) 812
HpyCH4III ACNGT 2 cut(s) 283, 1057
HpyCH4IV ACGT 2 cut(s) 37, 1337
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 233
HpyF3I CTNAG 2 cut(s) 204, 501
HpySE526I ACGT 2 cut(s) 37, 1337
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1076
HspAI GCGC 1 cut(s) 773
KspAI GTTAAC 1 cut(s) 1341
Kzo9I GATC 2 cut(s) 71, 526
LmnI GCTCC 1 cut(s) 924
Lsp1109I GCAGC 6 cut(s) 79, 114, 359, 704, 721, 1009
LweI GCATC 7 cut(s) 178, 426, 462, 694, 819, 1301, 1383
MaeI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1049, 1139, 1226, 1361
MaeII ACGT 2 cut(s) 37, 1337
MaeIII GTNAC 5 cut(s) 166, 327, 971, 1051, 1114
MalI GATC 2 cut(s) 73, 528
MboI GATC 2 cut(s) 71, 526
MboII GAAGA 9 cut(s) 59, 66, 187, 392, 395, 498, 616, 647, 1214
MflI RGATCY 2 cut(s) 71, 526
MhlI GDGCHC 2 cut(s) 771, 799
MnlI CCTC 4 cut(s) 889, 944, 991, 1135
Mph1103I ATGCAT 3 cut(s) 449, 455, 1333
MseI TTAA 4 cut(s) 272, 762, 839, 1340
MslI CAYNNNNRTG 1 cut(s) 448
MspA1I CMGCKG 1 cut(s) 695
MspR9I CCNGG 3 cut(s) 947, 1179, 1353
MssI GTTTAAAC 1 cut(s) 840
Mva1269I GAATGC 1 cut(s) 1331
MvaI CCWGG 3 cut(s) 947, 1179, 1353
MvnI CGCG 2 cut(s) 773, 775
MwoI GCNNNNNNNGC 2 cut(s) 224, 233
NdeI CATATG 1 cut(s) 449
NdeII GATC 2 cut(s) 71, 526
NheI GCTAGC 1 cut(s) 1225
NlaIII CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1076
NlaIV GGNNCC 1 cut(s) 261
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 3 cut(s) 449, 455, 1333
NspI RCATGY 2 cut(s) 447, 931
PcsI WCGNNNNNNNCGW 1 cut(s) 577
PctI GAATGC 1 cut(s) 1331
PfeI GAWTC 2 cut(s) 5, 392
PfoI TCCNGGA 1 cut(s) 1351
PkrI GCNGC 8 cut(s) 94, 129, 132, 349, 694, 736, 1024, 1373
PmeI GTTTAAAC 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 762
Psp6I CCWGG 3 cut(s) 945, 1177, 1351
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 3 cut(s) 945, 1177, 1351
PspN4I GGNNCC 1 cut(s) 261
PsuI RGATCY 2 cut(s) 71, 526
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 4 cut(s) 514, 708, 932, 1197
RsaNI GTAC 4 cut(s) 513, 707, 931, 1196
RseI CAYNNNNRTG 1 cut(s) 448
SaqAI TTAA 4 cut(s) 272, 762, 839, 1340
SatI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1023, 1372
Sau3AI GATC 2 cut(s) 71, 526
ScaI AGTACT 1 cut(s) 1197
ScrFI CCNGG 3 cut(s) 947, 1179, 1353
SduI GDGCHC 2 cut(s) 771, 799
SfaNI GCATC 7 cut(s) 178, 426, 462, 694, 819, 1301, 1383
SfcI CTRYAG 2 cut(s) 666, 1152
SmiMI CAYNNNNRTG 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 623
SsiI CCGC 6 cut(s) 130, 771, 775, 1130, 1216, 1371
SspI AATATT 1 cut(s) 1233
SspMI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1049, 1139, 1226, 1361
StyD4I CCNGG 3 cut(s) 945, 1177, 1351
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 2 cut(s) 283, 1057
TaiI ACGT 2 cut(s) 40, 1340
TatI WGTACW 2 cut(s) 706, 1195
TauI GCSGC 2 cut(s) 133, 1374
TfiI GAWTC 2 cut(s) 5, 392
Tru1I TTAA 4 cut(s) 272, 762, 839, 1340
Tru9I TTAA 4 cut(s) 272, 762, 839, 1340
TscAI CASTG 3 cut(s) 286, 716, 1125
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 6 cut(s) 92, 127, 347, 692, 734, 1022
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 4 cut(s) 241, 831, 876, 1336
TspRI CASTG 3 cut(s) 286, 716, 1125
VneI GTGCAC 1 cut(s) 795
VspI ATTAAT 1 cut(s) 762
XapI RAATTY 6 cut(s) 50, 583, 715, 786, 955, 1246
XbaI TCTAGA 2 cut(s) 673, 1138
XceI RCATGY 2 cut(s) 447, 931
XspI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1049, 1139, 1226, 1361
ZraI GACGTC 1 cut(s) 38
ZrmI AGTACT 1 cut(s) 1197
Zsp2I ATGCAT 3 cut(s) 449, 455, 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.