Rh7DG259400

GWT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005057
Physical Location & Seq
Reverse (-)
27279564 .. 27287297
7734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG259400.1

Sequence Viewer

Length: 1317 bp
ATGCTGGAAATCGCCGCCATCACCACAATAATCCCAGCTCTGTTTCTGCTGCGGCGAGCAGTCGGCTTCAATCGTCCTGCTGATGATGTCACATTGAAGAAAAATGATGATGCAAAAGTTGGTTCTAAGAGTTGGCAGGCTTACATGGCTAGGCTGGCTGTGGATTTTCTATTCATTATGGTTCCATTTGTCTTAATTTTCACTGTCTTATCAGAGTGGATGTATATCTGGGCAACTTTGCTAATTCTGTTACTTGTTTTCAGCATTGCAGCCAGAAGTTTTGGTTTTGGTTCTAATTTGGAAGAAGAACCCGATTCTATTAGGACAAGTATTACATCATTTAGGGTCGCAACGATGATGCTAACATGCGTATGTATCTTGGCTGTTGACTTCACAATTTTTCCTAGAAGATATGCCAAGACTGAGACTTATGGTACTGGCTGGATGGATCTTGGGGTTGGCTCATTTGTCGTAGCAAACTCATTTGTGTCTCGGCAAGCACGAAATTTATCATGGACGAAATGGAAGACTGCAATTCAATCTACTAGTCCACTAATCATCCTTGGGTTTGTTCGTCTTCTCTCTACTGCAGGTCTAGACTATCAGCATCATGCAGCTGAATATGGAGTACACTGGAATTTCTTTTTCACACTTGCTGCCATATCATTTCTTACATCCATAATTAATGTGCCCGCGCGGTATTCTGGAATTCTCGGTGCACTAATTCTAGTAGGTTACGAAGGTTGGTTGATGCAGGGGTTAAACACGTATCTGCTTTCTAATGAAAGAGGAAGTGATATAATTAGCCAAAATAAGGAGGGATTTTTTAGCATATTTGGATATTGGAGCATGTACCTCATTGGTGTCCAGGTGGGGAATTTTCTTTTTTTCAGTAACCACTCCTCTGCTACAACAAGAAGCCATAAATGGACAAGGAATAGAGCTGCAATGCTTTCACTTCTGTTTTGGCTAGTAACTGTGGTTATAGATAGCCATGTCCAGAGAGTTTCCCGTAGAATGTGCAATCTGGCTTATGTTACACTGGTATTGGCCGTAAATCTAGAGGGTATGGCCATAGTTCTGTTCTCTGATTGTTTCCCTGGAAGCAAAACATCAGTACTTGAAGAAGCATATAACCGCAATTTGCTAGCAATATTTCTTTTGGCAAATTTGCTTACTGGTCTGGTAAACTTTTTGGTGGATACGCTGTTTGCATCATCAGTCAAAGCTCTTTCTGTGATGATTGCCTATGCATTCACGTTAACTTTCATTCCTGGATTGCTAGACTTTTGCGGCATCCGATTTAGATTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
GWT1

Protein Analysis

438

Amino Acids

49.22

Weight (kDa)

9.15

Isoelectric Point (pI)

29.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 1 - 425 3.3e-122 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 581
AccII CGCG 2 cut(s) 695, 697
AciI CCGC 6 cut(s) 15, 52, 693, 697, 1138, 1293
AclWI GGATC 1 cut(s) 456
AcoI YGGCCR 2 cut(s) 1050, 1071
AcsI RAATTY 5 cut(s) 505, 637, 708, 877, 1168
AfaI GTAC 4 cut(s) 436, 630, 854, 1119
AfiI CCNNNNNNNGG 1 cut(s) 814
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 4 cut(s) 70, 97, 539, 1124
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 3 cut(s) 867, 1099, 1273
AluBI AGCT 4 cut(s) 38, 617, 944, 1229
AluI AGCT 4 cut(s) 38, 617, 944, 1229
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 2 cut(s) 419, 495
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 1 cut(s) 456
AoxI GGCC 2 cut(s) 1050, 1071
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 5 cut(s) 49, 269, 614, 656, 944
ApoI RAATTY 5 cut(s) 505, 637, 708, 877, 1168
AseI ATTAAT 1 cut(s) 684
AspLEI GCGC 1 cut(s) 697
AsuHPI GGTGA 1 cut(s) 13
AsuNHI GCTAGC 1 cut(s) 1147
BaeGI GKGCMC 2 cut(s) 693, 721
BalI TGGCCA 1 cut(s) 1073
BbsI GAAGAC 2 cut(s) 533, 569
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 5 cut(s) 36, 281, 626, 643, 931
BccI CCATC 2 cut(s) 26, 439
BceAI ACGGC 1 cut(s) 1037
BciT130I CCWGG 3 cut(s) 869, 1101, 1275
BciVI GTATCC 1 cut(s) 1195
BcoDI GTCTC 2 cut(s) 419, 495
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 9 cut(s) 150, 405, 546, 596, 728, 971, 1061, 1148, 1283
BfmI CTRYAG 1 cut(s) 588
BfuAI ACCTGC 1 cut(s) 581
BfuI GTATCC 1 cut(s) 1195
BisI GCNGC 8 cut(s) 15, 50, 53, 270, 615, 657, 945, 1294
BlsI GCNGC 8 cut(s) 16, 51, 54, 271, 616, 658, 946, 1295
BmcAI AGTACT 1 cut(s) 1119
Bme1390I CCNGG 3 cut(s) 869, 1101, 1275
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 3 cut(s) 869, 1101, 1275
BmsI GCATC 6 cut(s) 100, 348, 616, 741, 1223, 1305
BmtI GCTAGC 1 cut(s) 1151
BpiI GAAGAC 2 cut(s) 533, 569
BsaAI YACGTR 1 cut(s) 768
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 562, 1099
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse1I ACTGG 4 cut(s) 442, 638, 1047, 1183
Bse3DI GCAATG 2 cut(s) 264, 954
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 3 cut(s) 869, 1101, 1275
BseDI CCNNGG 2 cut(s) 562, 1099
BseGI GGATG 5 cut(s) 225, 450, 558, 674, 1296
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMI GCAATG 2 cut(s) 264, 954
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 4 cut(s) 442, 638, 1047, 1183
BseRI GAGGAG 1 cut(s) 892
BseSI GKGCMC 2 cut(s) 693, 721
BseXI GCAGC 5 cut(s) 36, 281, 626, 643, 931
BseYI CCCAGC 1 cut(s) 34
Bsh1236I CGCG 2 cut(s) 695, 697
BshFI GGCC 2 cut(s) 1052, 1073
BsiHKAI GWGCWC 1 cut(s) 721
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 2 cut(s) 419, 495
BsmI GAATGC 1 cut(s) 1253
BsnI GGCC 2 cut(s) 1052, 1073
Bsp1286I GDGCHC 2 cut(s) 693, 721
Bsp143I GATC 1 cut(s) 448
BspACI CCGC 6 cut(s) 15, 52, 693, 697, 1138, 1293
BspANI GGCC 2 cut(s) 1052, 1073
BspCNI CTCAG 1 cut(s) 415
BspFNI CGCG 2 cut(s) 695, 697
BspLI GGNNCC 1 cut(s) 183
BspMAI CTGCAG 1 cut(s) 592
BspMI ACCTGC 1 cut(s) 581
BspOI GCTAGC 1 cut(s) 1151
BspPI GGATC 1 cut(s) 456
BsrDI GCAATG 2 cut(s) 264, 954
BsrI ACTGG 4 cut(s) 442, 638, 1047, 1183
BssECI CCNNGG 2 cut(s) 562, 1099
BssMI GATC 1 cut(s) 448
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 3 cut(s) 869, 1101, 1275
Bst4CI ACNGT 2 cut(s) 205, 979
BstBAI YACGTR 1 cut(s) 768
BstC8I GCNNGC 6 cut(s) 57, 138, 156, 498, 693, 1149
BstDEI CTNAG 2 cut(s) 126, 423
BstF5I GGATG 5 cut(s) 225, 450, 558, 674, 1296
BstFNI CGCG 2 cut(s) 695, 697
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 1 cut(s) 451
BstMAI GTCTC 2 cut(s) 419, 495
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 2 cut(s) 146, 155
BstNI CCWGG 3 cut(s) 869, 1101, 1275
BstNSI RCATGY 2 cut(s) 369, 853
BstSCI CCNGG 3 cut(s) 867, 1099, 1273
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 2 cut(s) 693, 721
BstUI CGCG 2 cut(s) 695, 697
BstV1I GCAGC 5 cut(s) 36, 281, 626, 643, 931
BstV2I GAAGAC 2 cut(s) 533, 569
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuI GTATCC 1 cut(s) 1195
BsuRI GGCC 2 cut(s) 1052, 1073
BtsCI GGATG 5 cut(s) 225, 450, 558, 674, 1296
BtsIMutI CAGTG 3 cut(s) 201, 631, 1040
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 6 cut(s) 57, 138, 156, 498, 693, 1149
CfoI GCGC 1 cut(s) 697
Csp6I GTAC 4 cut(s) 435, 629, 853, 1118
CviAII CATG 6 cut(s) 145, 366, 513, 611, 850, 995
CviQI GTAC 4 cut(s) 435, 629, 853, 1118
DdeI CTNAG 2 cut(s) 126, 423
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
EaeI YGGCCR 2 cut(s) 1050, 1071
Eco130I CCWWGG 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 708
EcoRII CCWGG 3 cut(s) 867, 1099, 1273
EcoT14I CCWWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 1255
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 6 cut(s) 148, 369, 516, 614, 853, 998
FatI CATG 6 cut(s) 144, 365, 512, 610, 849, 994
FauI CCCGC 1 cut(s) 700
Fnu4HI GCNGC 8 cut(s) 15, 50, 53, 270, 615, 657, 945, 1294
FokI GGATG 5 cut(s) 232, 457, 545, 661, 1283
Fsp4HI GCNGC 8 cut(s) 15, 50, 53, 270, 615, 657, 945, 1294
FspBI CTAG 9 cut(s) 150, 405, 546, 596, 728, 971, 1061, 1148, 1283
GlaI GCGC 1 cut(s) 696
GluI GCNGC 8 cut(s) 15, 50, 53, 270, 615, 657, 945, 1294
GsaI CCCAGC 1 cut(s) 38
HaeIII GGCC 2 cut(s) 1052, 1073
HhaI GCGC 1 cut(s) 697
Hin1II CATG 6 cut(s) 148, 369, 516, 614, 853, 998
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HincII GTYRAC 2 cut(s) 388, 1263
HindII GTYRAC 2 cut(s) 388, 1263
HinfI GANTC 1 cut(s) 314
HpaI GTTAAC 1 cut(s) 1263
HphI GGTGA 1 cut(s) 13
Hpy166II GTNNAC 6 cut(s) 388, 551, 631, 719, 1189, 1263
Hpy188I TCNGA 3 cut(s) 214, 1090, 1301
Hpy188III TCNNGA 4 cut(s) 596, 705, 1000, 1061
Hpy8I GTNNAC 6 cut(s) 388, 551, 631, 719, 1189, 1263
HpyAV CCTTC 1 cut(s) 734
HpyCH4III ACNGT 2 cut(s) 205, 979
HpyCH4IV ACGT 2 cut(s) 767, 1259
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 155
HpyF3I CTNAG 2 cut(s) 126, 423
HpySE526I ACGT 2 cut(s) 767, 1259
Hsp92II CATG 6 cut(s) 148, 369, 516, 614, 853, 998
HspAI GCGC 1 cut(s) 695
KspAI GTTAAC 1 cut(s) 1263
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 1 cut(s) 846
Lsp1109I GCAGC 5 cut(s) 36, 281, 626, 643, 931
LweI GCATC 6 cut(s) 100, 348, 616, 741, 1223, 1305
MaeI CTAG 9 cut(s) 150, 405, 546, 596, 728, 971, 1061, 1148, 1283
MaeII ACGT 2 cut(s) 767, 1259
MaeIII GTNAC 6 cut(s) 88, 249, 734, 893, 973, 1036
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 7 cut(s) 109, 314, 317, 420, 538, 569, 1136
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 693, 721
MlsI TGGCCA 1 cut(s) 1073
MluNI TGGCCA 1 cut(s) 1073
MnlI CCTC 5 cut(s) 782, 811, 866, 913, 1057
Mox20I TGGCCA 1 cut(s) 1073
Mph1103I ATGCAT 1 cut(s) 1255
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 4 cut(s) 194, 684, 761, 1262
MslI CAYNNNNRTG 1 cut(s) 370
Msp20I TGGCCA 1 cut(s) 1073
MspA1I CMGCKG 1 cut(s) 617
MspR9I CCNGG 3 cut(s) 869, 1101, 1275
Mva1269I GAATGC 1 cut(s) 1253
MvaI CCWGG 3 cut(s) 869, 1101, 1275
MvnI CGCG 2 cut(s) 695, 697
MwoI GCNNNNNNNGC 2 cut(s) 146, 155
NdeII GATC 1 cut(s) 448
NheI GCTAGC 1 cut(s) 1147
NlaIII CATG 6 cut(s) 148, 369, 516, 614, 853, 998
NlaIV GGNNCC 1 cut(s) 183
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 88
NsiI ATGCAT 1 cut(s) 1255
NspI RCATGY 2 cut(s) 369, 853
PcsI WCGNNNNNNNCGW 1 cut(s) 499
PctI GAATGC 1 cut(s) 1253
PfeI GAWTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 1273
PkrI GCNGC 8 cut(s) 16, 51, 54, 271, 616, 658, 946, 1295
Ppu21I YACGTR 1 cut(s) 768
PshBI ATTAAT 1 cut(s) 684
Psp6I CCWGG 3 cut(s) 867, 1099, 1273
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 3 cut(s) 867, 1099, 1273
PspN4I GGNNCC 1 cut(s) 183
PstI CTGCAG 1 cut(s) 592
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 4 cut(s) 436, 630, 854, 1119
RsaNI GTAC 4 cut(s) 435, 629, 853, 1118
RseI CAYNNNNRTG 1 cut(s) 370
SaqAI TTAA 4 cut(s) 194, 684, 761, 1262
SatI GCNGC 8 cut(s) 15, 50, 53, 270, 615, 657, 945, 1294
Sau3AI GATC 1 cut(s) 448
ScaI AGTACT 1 cut(s) 1119
ScrFI CCNGG 3 cut(s) 869, 1101, 1275
SduI GDGCHC 2 cut(s) 693, 721
SfaNI GCATC 6 cut(s) 100, 348, 616, 741, 1223, 1305
SfcI CTRYAG 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 370
SpeI ACTAGT 1 cut(s) 545
SsiI CCGC 6 cut(s) 15, 52, 693, 697, 1138, 1293
SspI AATATT 1 cut(s) 1155
SspMI CTAG 9 cut(s) 150, 405, 546, 596, 728, 971, 1061, 1148, 1283
StyD4I CCNGG 3 cut(s) 867, 1099, 1273
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 2 cut(s) 205, 979
TaiI ACGT 2 cut(s) 770, 1262
TatI WGTACW 2 cut(s) 628, 1117
TauI GCSGC 3 cut(s) 17, 55, 1296
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 4 cut(s) 194, 684, 761, 1262
Tru9I TTAA 4 cut(s) 194, 684, 761, 1262
TscAI CASTG 3 cut(s) 208, 638, 1047
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 5 cut(s) 49, 269, 614, 656, 944
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 3 cut(s) 163, 798, 1258
TspRI CASTG 3 cut(s) 208, 638, 1047
VneI GTGCAC 1 cut(s) 717
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 5 cut(s) 505, 637, 708, 877, 1168
XbaI TCTAGA 2 cut(s) 595, 1060
XceI RCATGY 2 cut(s) 369, 853
XspI CTAG 9 cut(s) 150, 405, 546, 596, 728, 971, 1061, 1148, 1283
ZrmI AGTACT 1 cut(s) 1119
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.