FvH4_5g23251

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009043
Physical Location & Seq
Forward (+)
14602263 .. 14605048
2786 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23251.t1

Sequence Viewer

Length: 501 bp
ATGGAGTCTAATGAAGCTAAGCACCACTGTGGTGAAAGGTTAATTTTGTATATACATTTTCTGGTGGATACCATTGTTGAACTGAATGTACTCAGTCTCTTGGAGTTCTCTTATTGGTTCCCATTGCCAGTGGATATACTGGGTGCATATCCCCACATAGATATTAAAGACCCAGAGCACCCTTACTCTTTGGAAGAGCTCAAAGTAATTTCAGAAGATGCAATTGAAGTAGATGATGTTCGTGGTCATGTTAGGGTCACATTTACTCCTACTGTTGAACATTGCAGTATGGCAACAGTTATTGGTCTTTGCCTACGTGTTAAACTCTTAAGGAGCCTTCCTTCTCGATTCAAGGTCGACATTAGGGTGGCACCTGGATCTCATGCAACTGAAGCTTCAGTTAATAAGCAACTGAATGATAAAGAGCGGGTAGCAGCAGCATTGGAAAACCCAAATGTTGTGGAAATGGTTGACGAATGCCTCACTCCTTCATATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

167

Amino Acids

18.76

Weight (kDa)

5.03

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 55 - 121 1.1e-06 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AccBSI CCGCTC 1 cut(s) 427
AccI GTMKAC 1 cut(s) 357
AciI CCGC 1 cut(s) 427
AclWI GGATC 1 cut(s) 385
AcuI CTGAAG 2 cut(s) 381, 411
AfaI GTAC 1 cut(s) 90
AflII CTTAAG 1 cut(s) 328
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 4 cut(s) 80, 227, 278, 352
AjnI CCWGG 1 cut(s) 373
AleI CACNNNNGTG 2 cut(s) 27, 30
AluBI AGCT 3 cut(s) 17, 199, 395
AluI AGCT 3 cut(s) 17, 199, 395
Alw21I GWGCWC 2 cut(s) 180, 201
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 385
ApeKI GCWGC 2 cut(s) 434, 437
AsuHPI GGTGA 1 cut(s) 44
BaeI ACNNNNGTAYC 2 cut(s) 60, 93
BanI GGYRCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 180, 201
BbvI GCAGC 2 cut(s) 446, 449
BciT130I CCWGG 1 cut(s) 375
BciVI GTATCC 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 101
BfrI CTTAAG 1 cut(s) 328
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 2 cut(s) 435, 438
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 2 cut(s) 436, 439
Bme1390I CCNGG 1 cut(s) 375
BmiI GGNNCC 3 cut(s) 119, 335, 372
BmrFI CCNGG 1 cut(s) 375
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 208
BmuI ACTGGG 1 cut(s) 149
Bpu1102I GCTNAGC 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bse1I ACTGG 2 cut(s) 128, 144
Bse3DI GCAATG 2 cut(s) 122, 280
BseBI CCWGG 1 cut(s) 375
BseMI GCAATG 2 cut(s) 122, 280
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 2 cut(s) 128, 144
BseXI GCAGC 2 cut(s) 446, 449
BshNI GGYRCC 1 cut(s) 370
BsiHKAI GWGCWC 2 cut(s) 180, 201
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 482
Bsp1286I GDGCHC 2 cut(s) 180, 201
Bsp143I GATC 1 cut(s) 377
Bsp1720I GCTNAGC 1 cut(s) 18
BspACI CCGC 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 105
BspLI GGNNCC 3 cut(s) 119, 335, 372
BspPI GGATC 1 cut(s) 385
BspQI GCTCTTC 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 370
BspTI CTTAAG 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 427
BsrDI GCAATG 2 cut(s) 122, 280
BsrI ACTGG 2 cut(s) 128, 144
BssMI GATC 1 cut(s) 377
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 3 cut(s) 29, 274, 298
Bst6I CTCTTC 1 cut(s) 189
BstAFI CTTAAG 1 cut(s) 328
BstBAI YACGTR 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 18, 92
BstKTI GATC 1 cut(s) 380
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 1 cut(s) 377
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 446, 449
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BsuI GTATCC 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 25, 135
Csp6I GTAC 1 cut(s) 89
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 2 cut(s) 248, 383
CviJI RGCY 4 cut(s) 17, 199, 336, 395
CviKI_1 RGCY 4 cut(s) 17, 199, 336, 395
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 2 cut(s) 18, 92
DpnI GATC 1 cut(s) 379
DpnII GATC 1 cut(s) 377
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 2 cut(s) 381, 411
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 2 cut(s) 251, 386
FatI CATG 2 cut(s) 247, 382
FauI CCCGC 1 cut(s) 420
FauNDI CATATG 1 cut(s) 493
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 435, 438
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 435, 438
GluI GCNGC 2 cut(s) 435, 438
Hin1II CATG 2 cut(s) 251, 386
HincII GTYRAC 2 cut(s) 358, 472
HindII GTYRAC 2 cut(s) 358, 472
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 2 cut(s) 5, 348
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 2 cut(s) 358, 472
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 345
Hpy8I GTNNAC 2 cut(s) 358, 472
HpyAV CCTTC 3 cut(s) 347, 351, 498
HpyCH4III ACNGT 3 cut(s) 29, 274, 298
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 4 cut(s) 146, 221, 285, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 2 cut(s) 18, 92
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 2 cut(s) 251, 386
Kzo9I GATC 1 cut(s) 377
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 333
LpnPI CCDG 6 cut(s) 47, 125, 141, 186, 360, 387
Lsp1109I GCAGC 2 cut(s) 446, 449
LweI GCATC 1 cut(s) 208
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 1 cut(s) 256
MalI GATC 1 cut(s) 379
MbiI CCGCTC 1 cut(s) 427
MboI GATC 1 cut(s) 377
MboII GAAGA 2 cut(s) 206, 227
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 2 cut(s) 180, 201
MluCI AATT 3 cut(s) 42, 207, 222
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 1 cut(s) 491
MseI TTAA 5 cut(s) 41, 165, 321, 329, 402
MslI CAYNNNNRTG 4 cut(s) 27, 30, 365, 496
MspCI CTTAAG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 375
MunI CAATTG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 482
MvaI CCWGG 1 cut(s) 375
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeI CATATG 1 cut(s) 493
NdeII GATC 1 cut(s) 377
NlaIII CATG 2 cut(s) 251, 386
NlaIV GGNNCC 3 cut(s) 119, 335, 372
NmuCI GTSAC 1 cut(s) 256
OliI CACNNNNGTG 2 cut(s) 27, 30
PciSI GCTCTTC 1 cut(s) 189
PctI GAATGC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 348
PkrI GCNGC 2 cut(s) 436, 439
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 3 cut(s) 119, 335, 372
PsrI GAACNNNNNNTAC 4 cut(s) 72, 104, 222, 254
PsuI RGATCY 1 cut(s) 377
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 4 cut(s) 27, 30, 365, 496
SacI GAGCTC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 356
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 5 cut(s) 41, 165, 321, 329, 402
SatI GCNGC 2 cut(s) 435, 438
Sau3AI GATC 1 cut(s) 377
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 2 cut(s) 180, 201
SetI ASST 7 cut(s) 19, 41, 201, 319, 357, 376, 397
SfaNI GCATC 1 cut(s) 208
SmiMI CAYNNNNRTG 4 cut(s) 27, 30, 365, 496
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
Sse9I AATT 3 cut(s) 42, 207, 222
SsiI CCGC 1 cut(s) 427
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 373
TaaI ACNGT 3 cut(s) 29, 274, 298
TaiI ACGT 1 cut(s) 319
TaqI TCGA 2 cut(s) 346, 357
TasI AATT 3 cut(s) 42, 207, 222
TatI WGTACW 1 cut(s) 88
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 5 cut(s) 41, 165, 321, 329, 402
Tru9I TTAA 5 cut(s) 41, 165, 321, 329, 402
TscAI CASTG 2 cut(s) 32, 135
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 2 cut(s) 434, 437
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 2 cut(s) 27, 480
TspRI CASTG 2 cut(s) 32, 135
Vha464I CTTAAG 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.