RLG00000002916

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009043
Physical Location & Seq
Reverse (-)
41711902 .. 41717966
6065 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002916

Sequence Viewer

Length: 537 bp
ATGCAGGACCTTGTTTCTGCAAAATCGAATCGATCAACAACGTCAACAGAAAAAATGGTATCTGAGTTAATAAATGCGAATCCAGTCATCTATGAAAAGAAAGAGCGGCGTGTTCGTAGTGTTCCAAGTGCTGCAGATGAATATGCTGTTGAACCAATTGACCAAGAGGAAATTTTTGATATCCTTTCTGATGATTATATTAAAGACCCGGAACACCCTTACTCTTTGGAAGAGCTCAAAGTAATTTCAGAAGATGCAATTGAAGTAGATGATGGTCGTGGTCATGTTAGGGTCACATTTACTCCTACTGTTGAACATTGCAGTATGGCAACAGTTATTGGTCTTTGCATACGTGTTAAACTCTTAAGGAGCCTGCCTTCTCGTTTCAAGGTGGACATTAGGGTGGCACCTGGATCTCATGCAACTGAAGCTGCAGTTAATAAGCAACTGAATGATAAAGAGCGGGTAGCAGCGGCATTGGAAAACCCAAATGTTGTGGATATGGTTGATGAATGCCTTACTCCTTCATATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

179

Amino Acids

19.89

Weight (kDa)

4.8

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 67 - 133 3.6e-07 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 406
AccBSI CCGCTC 2 cut(s) 106, 463
AciI CCGC 3 cut(s) 106, 463, 473
AclWI GGATC 1 cut(s) 421
AcsI RAATTY 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 447
AflII CTTAAG 1 cut(s) 364
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 4 cut(s) 152, 263, 314, 388
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 2 cut(s) 235, 431
AluI AGCT 2 cut(s) 235, 431
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 1 cut(s) 421
ApeKI GCWGC 3 cut(s) 131, 431, 470
ApoI RAATTY 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 7
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 118, 418, 482
BccI CCATC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 411
BcnI CCSGG 1 cut(s) 209
BfmI CTRYAG 2 cut(s) 132, 432
BfrI CTTAAG 1 cut(s) 364
BisI GCNGC 5 cut(s) 107, 132, 432, 471, 474
BlsI GCNGC 5 cut(s) 108, 133, 433, 472, 475
Bme1390I CCNGG 2 cut(s) 209, 411
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 2 cut(s) 371, 408
BmrFI CCNGG 2 cut(s) 209, 411
BmsI GCATC 1 cut(s) 244
BpuMI CCSGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 31
BsaAI YACGTR 1 cut(s) 353
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bse1I ACTGG 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 411
BseCI ATCGAT 1 cut(s) 31
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 83
BseXI GCAGC 3 cut(s) 118, 418, 482
BshNI GGYRCC 1 cut(s) 406
BshVI ATCGAT 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 1 cut(s) 209
BsmI GAATGC 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 32, 413
BspACI CCGC 3 cut(s) 106, 463, 473
BspCNI CTCAG 1 cut(s) 55
BspDI ATCGAT 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 371, 408
BspMAI CTGCAG 2 cut(s) 136, 436
BspPI GGATC 1 cut(s) 421
BspQI GCTCTTC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 406
BspTI CTTAAG 1 cut(s) 364
BsrBI CCGCTC 2 cut(s) 106, 463
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 1 cut(s) 83
BssMI GATC 2 cut(s) 32, 413
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 310, 334
Bst6I CTCTTC 1 cut(s) 225
BstAFI CTTAAG 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 63
BstKTI GATC 2 cut(s) 35, 416
BstMBI GATC 2 cut(s) 32, 413
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 2 cut(s) 207, 409
BstSFI CTRYAG 2 cut(s) 132, 432
BstV1I GCAGC 3 cut(s) 118, 418, 482
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
Bsu15I ATCGAT 1 cut(s) 31
BsuTUI ATCGAT 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 31
CspCI CAANNNNNGTGG 2 cut(s) 477, 512
CviAII CATG 2 cut(s) 284, 419
CviJI RGCY 3 cut(s) 235, 372, 431
CviKI_1 RGCY 3 cut(s) 235, 372, 431
DdeI CTNAG 1 cut(s) 63
DpnI GATC 2 cut(s) 34, 415
DpnII GATC 2 cut(s) 32, 413
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 409
EcoRV GATATC 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 2 cut(s) 287, 422
FatI CATG 2 cut(s) 283, 418
FauI CCCGC 1 cut(s) 456
FauNDI CATATG 1 cut(s) 529
Fnu4HI GCNGC 5 cut(s) 107, 132, 432, 471, 474
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 107, 132, 432, 471, 474
GluI GCNGC 5 cut(s) 107, 132, 432, 471, 474
HapII CCGG 1 cut(s) 209
Hin1II CATG 2 cut(s) 287, 422
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 2 cut(s) 28, 79
HpaII CCGG 1 cut(s) 209
Hpy166II GTNNAC 2 cut(s) 45, 394
Hpy188I TCNGA 3 cut(s) 64, 190, 250
Hpy8I GTNNAC 2 cut(s) 45, 394
HpyAV CCTTC 2 cut(s) 387, 534
HpyCH4III ACNGT 2 cut(s) 310, 334
HpyCH4IV ACGT 2 cut(s) 41, 352
HpyCH4V TGCA 8 cut(s) 4, 20, 134, 257, 321, 348, 422, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 2 cut(s) 41, 352
Hsp92II CATG 2 cut(s) 287, 422
Kzo9I GATC 2 cut(s) 32, 413
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 369
LpnPI CCDG 5 cut(s) 96, 222, 386, 396, 423
Lsp1109I GCAGC 3 cut(s) 118, 418, 482
LweI GCATC 1 cut(s) 244
MaeII ACGT 2 cut(s) 41, 352
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 34, 415
MbiI CCGCTC 2 cut(s) 106, 463
MboI GATC 2 cut(s) 32, 413
MboII GAAGA 2 cut(s) 242, 263
MfeI CAATTG 2 cut(s) 156, 258
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 4 cut(s) 156, 171, 243, 258
MnlI CCTC 1 cut(s) 160
MseI TTAA 5 cut(s) 68, 201, 357, 365, 438
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 473
MspCI CTTAAG 1 cut(s) 364
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 209, 411
MunI CAATTG 2 cut(s) 156, 258
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 428
NciI CCSGG 1 cut(s) 209
NdeI CATATG 1 cut(s) 529
NdeII GATC 2 cut(s) 32, 413
NlaIII CATG 2 cut(s) 287, 422
NlaIV GGNNCC 2 cut(s) 371, 408
NmuCI GTSAC 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 225
PctI GAATGC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 28, 79
PkrI GCNGC 5 cut(s) 108, 133, 433, 472, 475
Ppu21I YACGTR 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 7
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 2 cut(s) 371, 408
PspPI GGNCC 1 cut(s) 7
PspPPI RGGWCCY 1 cut(s) 7
PstI CTGCAG 2 cut(s) 136, 436
PsuI RGATCY 1 cut(s) 413
RseI CAYNNNNRTG 1 cut(s) 401
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 5 cut(s) 68, 201, 357, 365, 438
SatI GCNGC 5 cut(s) 107, 132, 432, 471, 474
Sau3AI GATC 2 cut(s) 32, 413
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 209, 411
SduI GDGCHC 1 cut(s) 237
SetI ASST 7 cut(s) 12, 44, 237, 355, 393, 412, 433
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 2 cut(s) 132, 432
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
Sse9I AATT 4 cut(s) 156, 171, 243, 258
SsiI CCGC 3 cut(s) 106, 463, 473
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 207, 409
TaaI ACNGT 2 cut(s) 310, 334
TaiI ACGT 2 cut(s) 44, 355
TaqI TCGA 2 cut(s) 26, 31
TasI AATT 4 cut(s) 156, 171, 243, 258
TauI GCSGC 2 cut(s) 109, 476
TfiI GAWTC 2 cut(s) 28, 79
Tru1I TTAA 5 cut(s) 68, 201, 357, 365, 438
Tru9I TTAA 5 cut(s) 68, 201, 357, 365, 438
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 131, 431, 470
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 4 cut(s) 108, 153, 516, 525
Vha464I CTTAAG 1 cut(s) 364
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.