Rroxscaffold_3G00247040

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009043
Physical Location & Seq
Reverse (-)
40373674 .. 40379357
5684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247040.1

Sequence Viewer

Length: 477 bp
ATGGTATCCGAGTTAATAAATGCGAATCCAGTCATCTATGAAAAGAAAGAGCGGCGGGTTCGTAGTGTTCCAAGTGCTGCAGATGAATATGCTGTTGAACCAATTGACCAAGAAGAAATTTTTGATCATATTAGAGATATTAAAGACCCGGAACACCCTTACTCTTTGGAAGAGCTCAAAGTAATTTCAGAAGATGCAATTGAAGTAGATGATGGTCGTGGTCATGTTAGGGTCACATTTACTCCTACTGTTGAACATTGCAGTATGGCAACAGTTATTGGTCTTTGCATACGTGTTAAACTCTTGAGGAGCCTGCCTTCTCGTTTCAAGGTGGACATTAGGGTGGCACCTGGATCTCATGCAACTGAAGCAGCAGTTAATAAGCAACTGAATGATAAAGAGCGGGTAGCTGCGGCATTGGAAAACCCAAATGTTGTGGAAATGGTTGATGAATGCCTTACTCCTTCATATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

158

Amino Acids

17.75

Weight (kDa)

4.91

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 38 - 113 3e-09 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccBSI CCGCTC 2 cut(s) 52, 403
AciI CCGC 4 cut(s) 52, 55, 403, 413
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 1 cut(s) 117
AcuI CTGAAG 1 cut(s) 387
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 4 cut(s) 98, 203, 254, 328
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 2 cut(s) 175, 410
AluI AGCT 2 cut(s) 175, 410
Alw21I GWGCWC 1 cut(s) 177
AlwI GGATC 1 cut(s) 361
ApeKI GCWGC 3 cut(s) 77, 371, 410
ApoI RAATTY 1 cut(s) 117
AsuC2I CCSGG 1 cut(s) 149
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 3 cut(s) 64, 383, 397
BccI CCATC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 16
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 78
BfuI GTATCC 1 cut(s) 16
BisI GCNGC 5 cut(s) 53, 78, 372, 411, 414
BlsI GCNGC 5 cut(s) 54, 79, 373, 412, 415
Bme1390I CCNGG 2 cut(s) 149, 351
BmiI GGNNCC 2 cut(s) 311, 348
BmrFI CCNGG 2 cut(s) 149, 351
BmsI GCATC 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bse1I ACTGG 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 256
BseBI CCWGG 1 cut(s) 351
BseMI GCAATG 1 cut(s) 256
BseNI ACTGG 1 cut(s) 29
BseRI GAGGAG 1 cut(s) 322
BseXI GCAGC 3 cut(s) 64, 383, 397
BshNI GGYRCC 1 cut(s) 346
BsiHKAI GWGCWC 1 cut(s) 177
BsiSI CCGG 1 cut(s) 149
BsmI GAATGC 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 2 cut(s) 124, 353
BspACI CCGC 4 cut(s) 52, 55, 403, 413
BspLI GGNNCC 2 cut(s) 311, 348
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 361
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 346
BsrBI CCGCTC 2 cut(s) 52, 403
BsrDI GCAATG 1 cut(s) 256
BsrI ACTGG 1 cut(s) 29
BssMI GATC 2 cut(s) 124, 353
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 250, 274
Bst6I CTCTTC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 314
BstKTI GATC 2 cut(s) 127, 356
BstMBI GATC 2 cut(s) 124, 353
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 2 cut(s) 147, 349
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 3 cut(s) 64, 383, 397
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuI GTATCC 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 314
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 2 cut(s) 224, 359
CviJI RGCY 3 cut(s) 175, 312, 410
CviKI_1 RGCY 3 cut(s) 175, 312, 410
DpnI GATC 2 cut(s) 126, 355
DpnII GATC 2 cut(s) 124, 353
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 177
FaeI CATG 2 cut(s) 227, 362
FaiI YATR 9 cut(s) 39, 90, 129, 225, 266, 290, 360, 469, 471
FatI CATG 2 cut(s) 223, 358
FauI CCCGC 2 cut(s) 48, 396
FauNDI CATATG 1 cut(s) 469
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 5 cut(s) 53, 78, 372, 411, 414
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 5 cut(s) 53, 78, 372, 411, 414
GluI GCNGC 5 cut(s) 53, 78, 372, 411, 414
HapII CCGG 1 cut(s) 149
Hin1II CATG 2 cut(s) 227, 362
HinfI GANTC 1 cut(s) 25
HpaII CCGG 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 10, 190
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 2 cut(s) 327, 474
HpyCH4III ACNGT 2 cut(s) 250, 274
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 5 cut(s) 80, 197, 261, 288, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 2 cut(s) 227, 362
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 2 cut(s) 124, 353
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 5 cut(s) 42, 162, 326, 336, 363
Lsp1109I GCAGC 3 cut(s) 64, 383, 397
LweI GCATC 1 cut(s) 184
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 232
MalI GATC 2 cut(s) 126, 355
MbiI CCGCTC 2 cut(s) 52, 403
MboI GATC 2 cut(s) 124, 353
MboII GAAGA 3 cut(s) 125, 182, 203
MfeI CAATTG 2 cut(s) 102, 198
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 4 cut(s) 102, 117, 183, 198
MnlI CCTC 1 cut(s) 300
MseI TTAA 4 cut(s) 14, 141, 297, 378
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 149, 351
MunI CAATTG 2 cut(s) 102, 198
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 368
NciI CCSGG 1 cut(s) 149
NdeI CATATG 1 cut(s) 469
NdeII GATC 2 cut(s) 124, 353
NlaIII CATG 2 cut(s) 227, 362
NlaIV GGNNCC 2 cut(s) 311, 348
NmuCI GTSAC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 25
PkrI GCNGC 5 cut(s) 54, 79, 373, 412, 415
Ppu21I YACGTR 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 311, 348
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 4 cut(s) 14, 141, 297, 378
SatI GCNGC 5 cut(s) 53, 78, 372, 411, 414
Sau3AI GATC 2 cut(s) 124, 353
ScrFI CCNGG 2 cut(s) 149, 351
SduI GDGCHC 1 cut(s) 177
SetI ASST 5 cut(s) 177, 295, 333, 352, 412
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 4 cut(s) 102, 117, 183, 198
SsiI CCGC 4 cut(s) 52, 55, 403, 413
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 147, 349
TaaI ACNGT 2 cut(s) 250, 274
TaiI ACGT 1 cut(s) 295
TasI AATT 4 cut(s) 102, 117, 183, 198
TauI GCSGC 2 cut(s) 55, 416
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 4 cut(s) 14, 141, 297, 378
Tru9I TTAA 4 cut(s) 14, 141, 297, 378
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 3 cut(s) 77, 371, 410
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 4 cut(s) 54, 99, 456, 465
XapI RAATTY 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.