FvH4_5g24100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009161
Physical Location & Seq
Reverse (-)
15388962 .. 15392411
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24100.t1

Sequence Viewer

Length: 1164 bp
ATGGCCGAGAGCCAACCACAAGACGACCCAGCAGACCTCAAGAAGCTCATGTGCTCCTACCTCGGCCTCAGCCTCAGCATGTTCCTCGCCCTAGCTCCCCACAAGTTCCAGGAGCTCCAGCTCCAGGTCCGCGAGCTCTCCAAGCGGCTGTGGCAGGCGGAGGAGCAGGTGCGCCAGATGAGATCGAGGCGAAAGGAGGACTCCAAGGCCAACGCCAGAGTCGTTGAGATCTTCGCCAGCCACCGCAACGCGTGGCAGGCCGAGGAGAAGCGGCTGCTGAGCCAGATCGACGCGGCGGAGAGGGAAATGGCGAGTCTGAATGCGAGGGTGGAGGAGCTGGAGAAGAGCCGGGAGGACTCGGAGTCGAGGATTTGTGAGTTGGAGAGGGAGGTTGGGGAGAGAGATGATATGATTGGGTTTATGGCCACTAGAGGAGAAGGCGGAGAGGCAGCGGCGCAGCAGCGGGGACTTTGTCACCAGTTCAAGGATGTTAATGTGTATGACAATGCCTATGCGTTTGGTTTCGACTCCGAATTGGTCGCCGAGGCTTCGAAACTCTGGCAGGATGTACAATGTGAATCCCTTGAACCATTGTATCATACGAAGCATTTTGTGCCAAGGGAGTCCCCATGGAAGGTTGATGGTGAATCGACTGGAGTATCATCTAAACTTAAAGTACTTGAACAGGAACTACTGAATTTGGAAAATATTGGAAAAAATGATCTTTCTAAGGTACCTTCAATAATGAGGAAGCAGACAAAGAGGTATCAAGCTCTTGCGGGGAAGATAGATGATCTGTGCCGAAGAATGCAGGCTAGTGATCCCTGTGAAGCCACAGTGAGTCCCGAGTTCCGTATGCAAAGACAAACAGAATTTCTACTTGAATCATTTAGACTTCAGCAACGTGCAACAGACACCGCACAGAAGCTGATGGCAGTGCAAACTGAGATAGGGAAAAGTTATTATGGGGATGAGCTGGGGAATCATGCCAAAGTTACGACGAGACGGTCTTTGGATTCCATCAGAAACAATTTCAGAGAACTTCAAAGGAACTTAGAGATATGGTTGGCCAGAATTACTGGAGATCTTGAGGGAATTCTTGCCAGAGATGGTGCTTCTCGTGTACGAGAATATTATGTATCTAGATATCCTTTTGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

44.61

Weight (kDa)

5.89

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
AasI GACNNNNNNGTC 1 cut(s) 1006
Acc36I ACCTGC 1 cut(s) 157
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccII CGCG 3 cut(s) 132, 251, 293
AclWI GGATC 1 cut(s) 815
AcoI YGGCCR 3 cut(s) 3, 423, 1068
AcsI RAATTY 3 cut(s) 697, 872, 1095
AcuI CTGAAG 1 cut(s) 881
AfaI GTAC 4 cut(s) 570, 678, 735, 1125
AfiI CCNNNNNNNGG 3 cut(s) 124, 484, 634
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 7 cut(s) 484, 587, 683, 741, 884, 1046, 1160
AhdI GACNNNNNGTC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 108, 123
AluBI AGCT 9 cut(s) 46, 95, 115, 121, 136, 337, 773, 928, 976
AluI AGCT 9 cut(s) 46, 95, 115, 121, 136, 337, 773, 928, 976
Alw21I GWGCWC 3 cut(s) 56, 117, 138
Alw26I GTCTC 1 cut(s) 997
AlwI GGATC 1 cut(s) 815
AlwNI CAGNNNCTG 1 cut(s) 928
Ama87I CYCGRG 1 cut(s) 845
AoxI GGCC 6 cut(s) 3, 64, 207, 258, 423, 1068
ApeKI GCWGC 4 cut(s) 274, 449, 457, 460
ApoI RAATTY 3 cut(s) 697, 872, 1095
Asp718I GGTACC 1 cut(s) 733
AspLEI GCGC 2 cut(s) 174, 457
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 467, 656
AsuII TTCGAA 1 cut(s) 551
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 1 cut(s) 127
BaeI ACNNNNGTAYC 4 cut(s) 578, 611, 642, 675
BalI TGGCCA 2 cut(s) 425, 1070
BanI GGYRCC 1 cut(s) 733
BanII GRGCYC 2 cut(s) 117, 138
BauI CACGAG 1 cut(s) 1119
Bbv12I GWGCWC 3 cut(s) 56, 117, 138
BbvCI CCTCAGC 2 cut(s) 68, 74
BbvI GCAGC 4 cut(s) 261, 461, 469, 472
BccI CCATC 4 cut(s) 635, 925, 1028, 1103
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 110, 125
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 997
BfaI CTAG 4 cut(s) 92, 429, 816, 1143
BfuAI ACCTGC 1 cut(s) 157
BglII AGATCT 2 cut(s) 228, 1084
BisI GCNGC 8 cut(s) 146, 272, 275, 294, 450, 453, 458, 461
BlpI GCTNAGC 1 cut(s) 278
BlsI GCNGC 8 cut(s) 147, 273, 276, 295, 451, 454, 459, 462
BmcAI AGTACT 1 cut(s) 678
Bme1390I CCNGG 3 cut(s) 110, 125, 350
Bme18I GGWCC 1 cut(s) 127
BmeRI GACNNNNNGTC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 845
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 1 cut(s) 735
BmrFI CCNGG 3 cut(s) 110, 125, 350
BpmI CTGGAG 5 cut(s) 101, 107, 359, 675, 1101
Bpu10I CCTNAGC 2 cut(s) 68, 74
Bpu1102I GCTNAGC 1 cut(s) 278
Bpu14I TTCGAA 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 23, 1109
BpuMI CCSGG 1 cut(s) 350
BsaJI CCNNGG 6 cut(s) 61, 204, 261, 543, 617, 629
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 484, 634
Bse1I ACTGG 3 cut(s) 478, 658, 1084
BseBI CCWGG 2 cut(s) 110, 125
BseDI CCNNGG 6 cut(s) 61, 204, 261, 543, 617, 629
BseGI GGATG 3 cut(s) 493, 571, 976
BseLI CCNNNNNNNGG 3 cut(s) 124, 484, 634
BseMII CTCAG 4 cut(s) 82, 88, 269, 936
BseNI ACTGG 3 cut(s) 478, 658, 1084
BseRI GAGGAG 4 cut(s) 176, 278, 347, 447
BseXI GCAGC 4 cut(s) 261, 461, 469, 472
BseYI CCCAGC 2 cut(s) 28, 976
Bsh1236I CGCG 3 cut(s) 132, 251, 293
BshFI GGCC 6 cut(s) 5, 66, 209, 260, 425, 1070
BshNI GGYRCC 1 cut(s) 733
BsiHKAI GWGCWC 3 cut(s) 56, 117, 138
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 1 cut(s) 349
BslFI GGGAC 3 cut(s) 480, 610, 828
BslI CCNNNNNNNGG 3 cut(s) 124, 484, 634
BsmAI GTCTC 1 cut(s) 997
BsmBI CGTCTC 1 cut(s) 997
BsmFI GGGAC 3 cut(s) 480, 610, 828
BsmI GAATGC 2 cut(s) 325, 813
BsnI GGCC 6 cut(s) 5, 66, 209, 260, 425, 1070
BsoBI CYCGRG 1 cut(s) 845
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 3 cut(s) 56, 117, 138
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
Bsp1720I GCTNAGC 1 cut(s) 278
Bsp19I CCATGG 1 cut(s) 629
BspANI GGCC 6 cut(s) 5, 66, 209, 260, 425, 1070
BspCNI CTCAG 4 cut(s) 81, 87, 270, 937
BspFNI CGCG 3 cut(s) 132, 251, 293
BspLI GGNNCC 1 cut(s) 735
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 1 cut(s) 815
BspQI GCTCTTC 1 cut(s) 338
BspT104I TTCGAA 1 cut(s) 551
BspT107I GGYRCC 1 cut(s) 733
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 3 cut(s) 478, 658, 1084
BssECI CCNNGG 6 cut(s) 61, 204, 261, 543, 617, 629
BssMI GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 3 cut(s) 204, 617, 629
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 2 cut(s) 110, 125
Bst4CI ACNGT 2 cut(s) 838, 1008
Bst6I CTCTTC 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 613
BstAUI TGTACA 1 cut(s) 568
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 5 cut(s) 134, 156, 238, 258, 813
BstDEI CTNAG 6 cut(s) 68, 74, 278, 729, 945, 1054
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 3 cut(s) 493, 571, 976
BstFNI CGCG 3 cut(s) 132, 251, 293
BstHHI GCGC 2 cut(s) 174, 457
BstKTI GATC 7 cut(s) 185, 231, 288, 724, 796, 823, 1087
BstMAI GTCTC 1 cut(s) 997
BstMBI GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
BstMWI GCNNNNNNNGC 4 cut(s) 142, 151, 257, 613
BstNI CCWGG 2 cut(s) 110, 125
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 3 cut(s) 108, 123, 348
BstUI CGCG 3 cut(s) 132, 251, 293
BstV1I GCAGC 4 cut(s) 261, 461, 469, 472
BstX2I RGATCY 2 cut(s) 228, 1084
BstYI RGATCY 2 cut(s) 228, 1084
BsuRI GGCC 6 cut(s) 5, 66, 209, 260, 425, 1070
BtgI CCRYGG 1 cut(s) 629
BtsCI GGATG 3 cut(s) 493, 571, 976
BtsI GCAGTG 1 cut(s) 942
BtsIMutI CAGTG 2 cut(s) 843, 942
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 5 cut(s) 134, 156, 238, 258, 813
CaiI CAGNNNCTG 1 cut(s) 928
CfoI GCGC 2 cut(s) 174, 457
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 299
Csp6I GTAC 4 cut(s) 569, 677, 734, 1124
CviAII CATG 4 cut(s) 49, 79, 630, 986
CviQI GTAC 4 cut(s) 569, 677, 734, 1124
DdeI CTNAG 6 cut(s) 68, 74, 278, 729, 945, 1054
DpnI GATC 7 cut(s) 184, 230, 287, 723, 795, 822, 1086
DpnII GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
DrdI GACNNNNNNGTC 1 cut(s) 1006
DriI GACNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 1006
EaeI YGGCCR 3 cut(s) 3, 423, 1068
Eam1104I CTCTTC 1 cut(s) 338
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 338
EciI GGCGGA 3 cut(s) 173, 311, 456
Ecl136II GAGCTC 2 cut(s) 115, 136
Eco130I CCWWGG 3 cut(s) 204, 617, 629
Eco24I GRGCYC 2 cut(s) 117, 138
Eco32I GATATC 1 cut(s) 1148
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 2 cut(s) 115, 136
Eco57I CTGAAG 1 cut(s) 881
Eco88I CYCGRG 1 cut(s) 845
EcoICRI GAGCTC 2 cut(s) 115, 136
EcoRI GAATTC 1 cut(s) 1095
EcoRII CCWGG 2 cut(s) 108, 123
EcoRV GATATC 1 cut(s) 1148
EcoT14I CCWWGG 3 cut(s) 204, 617, 629
EcoT38I GRGCYC 2 cut(s) 117, 138
ErhI CCWWGG 3 cut(s) 204, 617, 629
Esp3I CGTCTC 1 cut(s) 997
FaeI CATG 4 cut(s) 52, 82, 633, 989
FaqI GGGAC 3 cut(s) 480, 610, 828
FatI CATG 4 cut(s) 48, 78, 629, 985
FauI CCCGC 2 cut(s) 456, 772
Fnu4HI GCNGC 8 cut(s) 146, 272, 275, 294, 450, 453, 458, 461
FokI GGATG 3 cut(s) 500, 578, 983
FriOI GRGCYC 2 cut(s) 117, 138
Fsp4HI GCNGC 8 cut(s) 146, 272, 275, 294, 450, 453, 458, 461
FspBI CTAG 4 cut(s) 92, 429, 816, 1143
GlaI GCGC 2 cut(s) 173, 456
GluI GCNGC 8 cut(s) 146, 272, 275, 294, 450, 453, 458, 461
GsaI CCCAGC 2 cut(s) 32, 980
GsuI CTGGAG 5 cut(s) 101, 107, 359, 675, 1101
HaeIII GGCC 6 cut(s) 5, 66, 209, 260, 425, 1070
HapII CCGG 1 cut(s) 349
HgaI GACGC 1 cut(s) 299
HhaI GCGC 2 cut(s) 174, 457
Hin1II CATG 4 cut(s) 52, 82, 633, 989
Hin6I GCGC 2 cut(s) 172, 455
HinP1I GCGC 2 cut(s) 172, 455
HpaII CCGG 1 cut(s) 349
HphI GGTGA 2 cut(s) 467, 656
Hpy166II GTNNAC 1 cut(s) 1124
Hpy188I TCNGA 5 cut(s) 318, 361, 532, 1025, 1037
Hpy188III TCNNGA 4 cut(s) 40, 845, 1088, 1143
Hpy8I GTNNAC 1 cut(s) 1124
Hpy99I CGWCG 2 cut(s) 293, 1003
HpyAV CCTTC 3 cut(s) 431, 628, 747
HpyCH4III ACNGT 2 cut(s) 838, 1008
HpyCH4IV ACGT 1 cut(s) 904
HpyCH4V TGCA 4 cut(s) 811, 859, 908, 940
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 151, 257, 613
HpyF3I CTNAG 6 cut(s) 68, 74, 278, 729, 945, 1054
HpySE526I ACGT 1 cut(s) 904
Hsp92II CATG 4 cut(s) 52, 82, 633, 989
HspAI GCGC 2 cut(s) 172, 455
KpnI GGTACC 1 cut(s) 737
Kzo9I GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 7 cut(s) 59, 100, 112, 120, 126, 163, 334
Lsp1109I GCAGC 4 cut(s) 261, 461, 469, 472
MaeI CTAG 4 cut(s) 92, 429, 816, 1143
MaeII ACGT 1 cut(s) 904
MaeIII GTNAC 2 cut(s) 473, 994
MalI GATC 7 cut(s) 184, 230, 287, 723, 795, 822, 1086
MboI GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
MboII GAAGA 4 cut(s) 223, 355, 796, 816
MflI RGATCY 2 cut(s) 228, 1084
MhlI GDGCHC 3 cut(s) 56, 117, 138
MlsI TGGCCA 2 cut(s) 425, 1070
MluCI AATT 6 cut(s) 533, 697, 872, 1030, 1074, 1095
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 2 cut(s) 425, 1070
MlyI GAGTC 8 cut(s) 194, 228, 322, 350, 371, 521, 632, 850
MmeI TCCRAC 1 cut(s) 360
Mox20I TGGCCA 2 cut(s) 425, 1070
MscI TGGCCA 2 cut(s) 425, 1070
MseI TTAA 2 cut(s) 492, 672
Msp20I TGGCCA 2 cut(s) 425, 1070
MspA1I CMGCKG 2 cut(s) 452, 463
MspI CCGG 1 cut(s) 349
MspR9I CCNGG 3 cut(s) 110, 125, 350
Mva1269I GAATGC 2 cut(s) 325, 813
MvaI CCWGG 2 cut(s) 110, 125
MvnI CGCG 3 cut(s) 132, 251, 293
MwoI GCNNNNNNNGC 4 cut(s) 142, 151, 257, 613
NciI CCSGG 1 cut(s) 350
NcoI CCATGG 1 cut(s) 629
NdeII GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
NlaIII CATG 4 cut(s) 52, 82, 633, 989
NlaIV GGNNCC 1 cut(s) 735
NmeAIII GCCGAG 4 cut(s) 31, 42, 286, 568
NmuCI GTSAC 1 cut(s) 473
NspI RCATGY 1 cut(s) 82
NspV TTCGAA 1 cut(s) 551
PaqCI CACCTGC 1 cut(s) 157
PciSI GCTCTTC 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 2 cut(s) 325, 813
PfeI GAWTC 5 cut(s) 578, 647, 884, 982, 1016
PflFI GACNNNGTC 1 cut(s) 471
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 8 cut(s) 147, 273, 276, 295, 451, 454, 459, 462
PleI GAGTC 8 cut(s) 194, 227, 321, 350, 370, 521, 631, 849
PpsI GAGTC 8 cut(s) 194, 227, 321, 350, 370, 521, 631, 849
Psp124BI GAGCTC 2 cut(s) 117, 138
Psp6I CCWGG 2 cut(s) 108, 123
PspFI CCCAGC 2 cut(s) 28, 976
PspGI CCWGG 2 cut(s) 108, 123
PspN4I GGNNCC 1 cut(s) 735
PspPI GGNCC 1 cut(s) 127
PsrI GAACNNNNNNTAC 2 cut(s) 675, 707
PstNI CAGNNNCTG 1 cut(s) 928
PsuI RGATCY 2 cut(s) 228, 1084
PsyI GACNNNGTC 1 cut(s) 471
RsaI GTAC 4 cut(s) 570, 678, 735, 1125
RsaNI GTAC 4 cut(s) 569, 677, 734, 1124
SacI GAGCTC 2 cut(s) 117, 138
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 2 cut(s) 492, 672
SatI GCNGC 8 cut(s) 146, 272, 275, 294, 450, 453, 458, 461
Sau3AI GATC 7 cut(s) 182, 228, 285, 721, 793, 820, 1084
Sau96I GGNCC 1 cut(s) 127
ScaI AGTACT 1 cut(s) 678
SchI GAGTC 8 cut(s) 194, 228, 322, 350, 371, 521, 632, 850
ScrFI CCNGG 3 cut(s) 110, 125, 350
SduI GDGCHC 3 cut(s) 56, 117, 138
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 1 cut(s) 127
SmlI CTYRAG 2 cut(s) 38, 1088
SmoI CTYRAG 2 cut(s) 38, 1088
Sse9I AATT 6 cut(s) 533, 697, 872, 1030, 1074, 1095
SspI AATATT 2 cut(s) 709, 1133
SspMI CTAG 4 cut(s) 92, 429, 816, 1143
SstI GAGCTC 2 cut(s) 117, 138
StyD4I CCNGG 3 cut(s) 108, 123, 348
StyI CCWWGG 3 cut(s) 204, 617, 629
TaaI ACNGT 2 cut(s) 838, 1008
TaiI ACGT 1 cut(s) 907
TaqI TCGA 6 cut(s) 185, 288, 365, 525, 551, 650
TasI AATT 6 cut(s) 533, 697, 872, 1030, 1074, 1095
TatI WGTACW 2 cut(s) 568, 676
TauI GCSGC 4 cut(s) 148, 274, 296, 455
TfiI GAWTC 5 cut(s) 578, 647, 884, 982, 1016
Tru1I TTAA 2 cut(s) 492, 672
Tru9I TTAA 2 cut(s) 492, 672
TscAI CASTG 2 cut(s) 843, 942
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 4 cut(s) 274, 449, 457, 460
Tsp45I GTSAC 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 2 cut(s) 843, 942
Tth111I GACNNNGTC 1 cut(s) 471
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 3 cut(s) 697, 872, 1095
XbaI TCTAGA 1 cut(s) 1142
XceI RCATGY 1 cut(s) 82
XspI CTAG 4 cut(s) 92, 429, 816, 1143
ZrmI AGTACT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.