pycom06g01380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009161
Physical Location & Seq
Reverse (-)
1392474 .. 1395329
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g01380.2

Sequence Viewer

Length: 1197 bp
ATGGCTGAAAACGGGCACAAACTACAACAACAGCAGCCAGAAGAAGAAGATGACACGAAGAAGCTATTGTGCTCCTACCTTGGCCTGAGCCTGGGCATATTCCTGGCTTTAGCTCCCGACAACTTCCAGGAGCTCCAGACCCAAGTGGGCGAGCTCTCTGCCCGGCTGTGGCAGGCGGAAGAGCAAGTCAGGTTCATGACTTCCCGCCGGAAAGAGGACTCCAAAGCTAACGCCAGGGTCGTCGAGATCTTCGCCAGCCACCGCAACGCTTGGCAAGCCGAGGAGAAGCGGCTGCTACACCAGATCGACGCCGCCCATCAAGAAATTGGCCACCTCCATGCCAGGATTCTGGAGCTGGAGAAAACCCAGGACGATTCCAGCGCCAGGATTCGGGATTTGGAGAGGGAGGTTGGGGAGAGGGACGACATGATTGGCTTCATGGCCACCAGAGGCGGAGGAGGAGCTGATGCTGAGGCTGTTGACGCCGACCACCACTGTCACCGCCACCAAGGCAAGGAGGAAGTCAATGTCTACGACAATGCCGTCGCCTTCAGCTTCGACTCCCACTTGGTAGCCGATGCTTCCAAGCTCTGGCAGGATGTACAGTATGAATCCCTTGAACCGTTGTATCACATGAAGCATTTTGTGCCAAGGGAGTCACCTTGGAAGCTAGATGGTGAGTCGACCAGAATATCCTCGAAGCTAAAATTACTTGAACAGGAATTACTGAATTTGGAAACTATTGGGAAGAGTGATCTTTCCAAGGTACCTTCATCGATGAGGAAGCAGACCAAGAGGTATCAAGCTCTTGCGGGGAAGATAGACGATCTGTGCAGAAGAATGCAAGGTAGTGATCCCTCCGAACCTACACTCAGTCCAGAGTTTCGTACACAAAGACAAACAGAGTTTTTACTCGAATCATTTAGACTTCAACAACGTGCAACGGAAACTGGACAGAAGCTGATGGCAGTACAAACGGAGATTGGGAAGAGTTCTTATGGGGACGAGCTGGGGAACCAGGCCAAACACAACACACGACGGTCTTTGGATTCGATCAGAAACAACTTGAAAGAAATACAAAGAAATTTGGAGATATGGCTGGCCCGAATCATCGGAGATCTTGAAGGTATTCTGGCTAGAGATGGCGCTTCTCGTGTAGGGGAATATTGTGTCTCTAGATATCCTTTTGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

399

Amino Acids

45.48

Weight (kDa)

5.76

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 542
Acc65I GGTACC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 766
AccB7I CCANNNNNTGG 1 cut(s) 591
AccI GTMKAC 2 cut(s) 531, 683
AciI CCGC 8 cut(s) 176, 205, 262, 289, 312, 453, 502, 812
AclWI GGATC 1 cut(s) 848
AcoI YGGCCR 2 cut(s) 328, 441
AcsI RAATTY 2 cut(s) 730, 1084
AcuI CTGAAG 1 cut(s) 535
AcyI GRCGYC 2 cut(s) 309, 483
AfaI GTAC 4 cut(s) 603, 768, 889, 972
AfiI CCNNNNNNNGG 7 cut(s) 91, 168, 214, 384, 390, 514, 591
AgsI TTSAA 5 cut(s) 620, 716, 932, 1069, 1124
AjnI CCWGG 8 cut(s) 90, 102, 126, 233, 341, 366, 383, 1017
Alw21I GWGCWC 3 cut(s) 74, 135, 156
Alw26I GTCTC 1 cut(s) 1177
AlwI GGATC 1 cut(s) 848
AlwNI CAGNNNCTG 1 cut(s) 961
AoxI GGCC 5 cut(s) 82, 328, 441, 1020, 1101
ApeKI GCWGC 2 cut(s) 34, 292
ApoI RAATTY 2 cut(s) 730, 1084
Asp700I GAANNNNTTC 1 cut(s) 1128
Asp718I GGTACC 1 cut(s) 766
AspLEI GCGC 2 cut(s) 383, 1148
AspS9I GGNCC 1 cut(s) 1102
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 491, 651, 689
BaeGI GKGCMC 1 cut(s) 18
BaeI ACNNNNGTAYC 2 cut(s) 611, 644
BalI TGGCCA 2 cut(s) 330, 443
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 2 cut(s) 135, 156
BauI CACGAG 1 cut(s) 1152
Bbv12I GWGCWC 3 cut(s) 74, 135, 156
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 46, 279
BccI CCATC 4 cut(s) 324, 668, 958, 1136
BceAI ACGGC 1 cut(s) 527
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 8 cut(s) 92, 104, 128, 235, 343, 368, 385, 1019
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 1177
BfaI CTAG 3 cut(s) 671, 1137, 1176
BfoI RGCGCY 2 cut(s) 384, 1149
BglI GCCNNNNNGGC 1 cut(s) 510
BglII AGATCT 2 cut(s) 246, 1117
BisI GCNGC 4 cut(s) 35, 290, 293, 312
BlsI GCNGC 4 cut(s) 36, 291, 294, 313
Bme1390I CCNGG 9 cut(s) 92, 104, 128, 163, 235, 343, 368, 385, 1019
BmgT120I GGNCC 1 cut(s) 1102
BmiI GGNNCC 2 cut(s) 768, 1016
BmrFI CCNGG 9 cut(s) 92, 104, 128, 163, 235, 343, 368, 385, 1019
BmsI GCATC 2 cut(s) 457, 568
BpmI CTGGAG 3 cut(s) 119, 371, 377
Bpu10I CCTNAGC 2 cut(s) 86, 471
BpuMI CCSGG 1 cut(s) 163
Bsa29I ATCGAT 1 cut(s) 776
BsaHI GRCGYC 2 cut(s) 309, 483
BsaJI CCNNGG 9 cut(s) 79, 91, 234, 279, 366, 508, 650, 662, 762
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 168, 214, 384, 390, 514, 591
Bse1I ACTGG 1 cut(s) 955
BseBI CCWGG 8 cut(s) 92, 104, 128, 235, 343, 368, 385, 1019
BseCI ATCGAT 1 cut(s) 776
BseDI CCNNGG 9 cut(s) 79, 91, 234, 279, 366, 508, 650, 662, 762
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 7 cut(s) 91, 168, 214, 384, 390, 514, 591
BseMII CTCAG 3 cut(s) 77, 462, 886
BseNI ACTGG 1 cut(s) 955
BseRI GAGGAG 3 cut(s) 296, 471, 474
BseSI GKGCMC 1 cut(s) 18
BseXI GCAGC 2 cut(s) 46, 279
BseYI CCCAGC 1 cut(s) 1009
BsgI GTGCAG 1 cut(s) 853
BshFI GGCC 5 cut(s) 84, 330, 443, 1022, 1103
BshNI GGYRCC 1 cut(s) 766
BshVI ATCGAT 1 cut(s) 776
BsiHKAI GWGCWC 3 cut(s) 74, 135, 156
BsiSI CCGG 2 cut(s) 163, 208
BslFI GGGAC 2 cut(s) 434, 1016
BslI CCNNNNNNNGG 7 cut(s) 91, 168, 214, 384, 390, 514, 591
BsmAI GTCTC 1 cut(s) 1177
BsmFI GGGAC 2 cut(s) 434, 1016
BsmI GAATGC 1 cut(s) 846
BsnI GGCC 5 cut(s) 84, 330, 443, 1022, 1103
Bsp1286I GDGCHC 4 cut(s) 18, 74, 135, 156
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
BspACI CCGC 8 cut(s) 176, 205, 262, 289, 312, 453, 502, 812
BspANI GGCC 5 cut(s) 84, 330, 443, 1022, 1103
BspCNI CTCAG 3 cut(s) 78, 463, 885
BspDI ATCGAT 1 cut(s) 776
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 768, 1016
BspPI GGATC 1 cut(s) 848
BspQI GCTCTTC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 766
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 1 cut(s) 955
BssECI CCNNGG 9 cut(s) 79, 91, 234, 279, 366, 508, 650, 662, 762
BssMI GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
BssNI GRCGYC 2 cut(s) 309, 483
BssSI CACGAG 1 cut(s) 1152
BssT1I CCWWGG 5 cut(s) 79, 508, 650, 662, 762
Bst2BI CACGAG 1 cut(s) 1152
Bst2UI CCWGG 8 cut(s) 92, 104, 128, 235, 343, 368, 385, 1019
Bst4CI ACNGT 4 cut(s) 497, 606, 624, 1041
Bst6I CTCTTC 3 cut(s) 174, 743, 983
BstACI GRCGYC 2 cut(s) 309, 483
BstAPI GCANNNNNTGC 1 cut(s) 646
BstAUI TGTACA 1 cut(s) 601
BstC8I GCNNGC 5 cut(s) 152, 174, 256, 276, 1101
BstDEI CTNAG 3 cut(s) 86, 471, 872
BstF5I GGATG 1 cut(s) 604
BstH2I RGCGCY 2 cut(s) 384, 1149
BstHHI GCGC 2 cut(s) 383, 1148
BstKTI GATC 7 cut(s) 249, 306, 757, 829, 856, 1056, 1120
BstMAI GTCTC 1 cut(s) 1177
BstMBI GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
BstMWI GCNNNNNNNGC 4 cut(s) 275, 482, 510, 646
BstNI CCWGG 8 cut(s) 92, 104, 128, 235, 343, 368, 385, 1019
BstSCI CCNGG 9 cut(s) 90, 102, 126, 161, 233, 341, 366, 383, 1017
BstSLI GKGCMC 1 cut(s) 18
BstV1I GCAGC 2 cut(s) 46, 279
BstX2I RGATCY 2 cut(s) 246, 1117
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 2 cut(s) 246, 1117
Bsu15I ATCGAT 1 cut(s) 776
BsuRI GGCC 5 cut(s) 84, 330, 443, 1022, 1103
BsuTUI ATCGAT 1 cut(s) 776
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 493
Cac8I GCNNGC 5 cut(s) 152, 174, 256, 276, 1101
CaiI CAGNNNCTG 1 cut(s) 961
CciI TCATGA 1 cut(s) 195
CfoI GCGC 2 cut(s) 383, 1148
Cfr13I GGNCC 1 cut(s) 1102
ClaI ATCGAT 1 cut(s) 776
CseI GACGC 2 cut(s) 317, 491
Csp6I GTAC 4 cut(s) 602, 767, 888, 971
CviAII CATG 5 cut(s) 196, 338, 427, 439, 634
CviQI GTAC 4 cut(s) 602, 767, 888, 971
DdeI CTNAG 3 cut(s) 86, 471, 872
DpnI GATC 7 cut(s) 248, 305, 756, 828, 855, 1055, 1119
DpnII GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
EaeI YGGCCR 2 cut(s) 328, 441
Eam1104I CTCTTC 3 cut(s) 174, 743, 983
EarI CTCTTC 3 cut(s) 174, 743, 983
EciI GGCGGA 2 cut(s) 191, 468
Ecl136II GAGCTC 2 cut(s) 133, 154
Eco130I CCWWGG 5 cut(s) 79, 508, 650, 662, 762
Eco24I GRGCYC 2 cut(s) 135, 156
Eco32I GATATC 1 cut(s) 1181
Eco53kI GAGCTC 2 cut(s) 133, 154
Eco57I CTGAAG 1 cut(s) 535
EcoICRI GAGCTC 2 cut(s) 133, 154
EcoRII CCWGG 8 cut(s) 90, 102, 126, 233, 341, 366, 383, 1017
EcoRV GATATC 1 cut(s) 1181
EcoT14I CCWWGG 5 cut(s) 79, 508, 650, 662, 762
EcoT38I GRGCYC 2 cut(s) 135, 156
ErhI CCWWGG 5 cut(s) 79, 508, 650, 662, 762
FaeI CATG 5 cut(s) 199, 341, 430, 442, 637
FaiI YATR 9 cut(s) 98, 197, 339, 428, 440, 609, 635, 999, 1096
FaqI GGGAC 2 cut(s) 434, 1016
FatI CATG 5 cut(s) 195, 337, 426, 438, 633
FauI CCCGC 2 cut(s) 212, 805
FblI GTMKAC 2 cut(s) 531, 683
Fnu4HI GCNGC 4 cut(s) 35, 290, 293, 312
FokI GGATG 1 cut(s) 611
FriOI GRGCYC 2 cut(s) 135, 156
Fsp4HI GCNGC 4 cut(s) 35, 290, 293, 312
FspBI CTAG 3 cut(s) 671, 1137, 1176
GlaI GCGC 2 cut(s) 382, 1147
GluI GCNGC 4 cut(s) 35, 290, 293, 312
GsaI CCCAGC 1 cut(s) 1013
GsuI CTGGAG 3 cut(s) 119, 371, 377
HaeII RGCGCY 2 cut(s) 384, 1149
HaeIII GGCC 5 cut(s) 84, 330, 443, 1022, 1103
HapII CCGG 2 cut(s) 163, 208
HgaI GACGC 2 cut(s) 317, 491
HhaI GCGC 2 cut(s) 383, 1148
Hin1I GRCGYC 2 cut(s) 309, 483
Hin1II CATG 5 cut(s) 199, 341, 430, 442, 637
Hin6I GCGC 2 cut(s) 381, 1146
HinP1I GCGC 2 cut(s) 381, 1146
HincII GTYRAC 2 cut(s) 481, 684
HindII GTYRAC 2 cut(s) 481, 684
HpaII CCGG 2 cut(s) 163, 208
HphI GGTGA 3 cut(s) 491, 651, 689
Hpy166II GTNNAC 4 cut(s) 481, 532, 684, 890
Hpy188I TCNGA 3 cut(s) 862, 1058, 1115
Hpy8I GTNNAC 4 cut(s) 481, 532, 684, 890
Hpy99I CGWCG 4 cut(s) 245, 311, 548, 1041
HpyAV CCTTC 3 cut(s) 559, 780, 1118
HpyCH4III ACNGT 4 cut(s) 497, 606, 624, 1041
HpyCH4IV ACGT 1 cut(s) 937
HpyCH4V TGCA 3 cut(s) 834, 844, 941
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 482, 510, 646
HpyF3I CTNAG 3 cut(s) 86, 471, 872
HpySE526I ACGT 1 cut(s) 937
Hsp92I GRCGYC 2 cut(s) 309, 483
Hsp92II CATG 5 cut(s) 199, 341, 430, 442, 637
HspAI GCGC 2 cut(s) 381, 1146
KpnI GGTACC 1 cut(s) 770
Kzo9I GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 6 cut(s) 77, 118, 130, 138, 352, 461
Lsp1109I GCAGC 2 cut(s) 46, 279
LweI GCATC 2 cut(s) 457, 568
MaeI CTAG 3 cut(s) 671, 1137, 1176
MaeII ACGT 1 cut(s) 937
MaeIII GTNAC 2 cut(s) 497, 657
MalI GATC 7 cut(s) 248, 305, 756, 828, 855, 1055, 1119
MboI GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
MflI RGATCY 2 cut(s) 246, 1117
MhlI GDGCHC 4 cut(s) 18, 74, 135, 156
MlsI TGGCCA 2 cut(s) 330, 443
MluCI AATT 5 cut(s) 324, 707, 722, 730, 1084
MluNI TGGCCA 2 cut(s) 330, 443
MlyI GAGTC 4 cut(s) 212, 554, 665, 689
Mox20I TGGCCA 2 cut(s) 330, 443
MroXI GAANNNNTTC 1 cut(s) 1128
MscI TGGCCA 2 cut(s) 330, 443
MslI CAYNNNNRTG 1 cut(s) 336
Msp20I TGGCCA 2 cut(s) 330, 443
MspI CCGG 2 cut(s) 163, 208
MspR9I CCNGG 9 cut(s) 92, 104, 128, 163, 235, 343, 368, 385, 1019
Mva1269I GAATGC 1 cut(s) 846
MvaI CCWGG 8 cut(s) 92, 104, 128, 235, 343, 368, 385, 1019
MwoI GCNNNNNNNGC 4 cut(s) 275, 482, 510, 646
NciI CCSGG 1 cut(s) 163
NdeII GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
NlaIII CATG 5 cut(s) 199, 341, 430, 442, 637
NlaIV GGNNCC 2 cut(s) 768, 1016
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 497, 657
PagI TCATGA 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 540
PctI GAATGC 1 cut(s) 846
PdmI GAANNNNTTC 1 cut(s) 1128
PfeI GAWTC 7 cut(s) 346, 374, 388, 611, 917, 1049, 1107
PflMI CCANNNNNTGG 1 cut(s) 591
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 4 cut(s) 36, 291, 294, 313
PleI GAGTC 4 cut(s) 212, 554, 664, 688
PpsI GAGTC 4 cut(s) 212, 554, 664, 688
Psp124BI GAGCTC 2 cut(s) 135, 156
Psp6I CCWGG 8 cut(s) 90, 102, 126, 233, 341, 366, 383, 1017
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 8 cut(s) 90, 102, 126, 233, 341, 366, 383, 1017
PspN4I GGNNCC 2 cut(s) 768, 1016
PspPI GGNCC 1 cut(s) 1102
PsrI GAACNNNNNNTAC 2 cut(s) 708, 740
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 2 cut(s) 246, 1117
RsaI GTAC 4 cut(s) 603, 768, 889, 972
RsaNI GTAC 4 cut(s) 602, 767, 888, 971
RseI CAYNNNNRTG 1 cut(s) 336
SacI GAGCTC 2 cut(s) 135, 156
SalI GTCGAC 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 174
SatI GCNGC 4 cut(s) 35, 290, 293, 312
Sau3AI GATC 7 cut(s) 246, 303, 754, 826, 853, 1053, 1117
Sau96I GGNCC 1 cut(s) 1102
SchI GAGTC 4 cut(s) 212, 554, 665, 689
ScrFI CCNGG 9 cut(s) 92, 104, 128, 163, 235, 343, 368, 385, 1019
SduI GDGCHC 4 cut(s) 18, 74, 135, 156
SfaNI GCATC 2 cut(s) 457, 568
SmiMI CAYNNNNRTG 1 cut(s) 336
Sse9I AATT 5 cut(s) 324, 707, 722, 730, 1084
SsiI CCGC 8 cut(s) 176, 205, 262, 289, 312, 453, 502, 812
SspI AATATT 1 cut(s) 1166
SspMI CTAG 3 cut(s) 671, 1137, 1176
SstI GAGCTC 2 cut(s) 135, 156
StyD4I CCNGG 9 cut(s) 90, 102, 126, 161, 233, 341, 366, 383, 1017
StyI CCWWGG 5 cut(s) 79, 508, 650, 662, 762
TaaI ACNGT 4 cut(s) 497, 606, 624, 1041
TaiI ACGT 1 cut(s) 940
TaqI TCGA 8 cut(s) 243, 306, 558, 683, 698, 776, 915, 1052
TasI AATT 5 cut(s) 324, 707, 722, 730, 1084
TatI WGTACW 2 cut(s) 601, 970
TauI GCSGC 2 cut(s) 292, 314
TfiI GAWTC 7 cut(s) 346, 374, 388, 611, 917, 1049, 1107
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 2 cut(s) 497, 657
TseI GCWGC 2 cut(s) 34, 292
Tsp45I GTSAC 2 cut(s) 497, 657
TspDTI ATGAA 5 cut(s) 184, 427, 624, 650, 762
TspGWI ACGGA 2 cut(s) 959, 992
TspRI CASTG 1 cut(s) 500
Van91I CCANNNNNTGG 1 cut(s) 591
XapI RAATTY 2 cut(s) 730, 1084
XbaI TCTAGA 1 cut(s) 1175
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 531, 683
XmnI GAANNNNTTC 1 cut(s) 1128
XspI CTAG 3 cut(s) 671, 1137, 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.