pycom16g25530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009161
Physical Location & Seq
Reverse (-)
27486147 .. 27489335
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g25530.3

Sequence Viewer

Length: 1206 bp
ATGGCTGAAAACGGGCACAAACTACAACAACAGCAACTAGAAGAAGAAGAAGATGATGCTACGAAGAAGCTATTCTGTTCCTACCTTGGCCTGAGCCTGGGTGTTTTCCTGGCTTTAGCTCCCGACAACTTCCAGGAGCTCCAGAGCCAAGTGGGCGAGCTCTCTGCGCGGCTATGGCAGGCGGAAGAGCAAGTGAGCTTCATGACTTCCAGGCGAAAAGAGGACTCCAAAGCCAATGCCAGGGTCGTCGAGATCGTCGCCAGCCACCGCAACGCTTGGCAAGCCGAGGAGAAGCGGCTGCTGAACCAGATCGACGACGCCCACCAAGAAATCGCCCACCTCCATGCCAGGATTCTGGAGCTGGAGAAAACCCAGGACGATTCCAGTGCCAGGATTCAGGATTTGGAGATGTTGGTTGAGGAGAGGGACGACATGATTGGTTTCATGGCCACCAGCAGCGGAGGAGGACCTGAGGCTGAGCTTGACGGCGACAACCACTGTCACCGCCACCAGCACCGAGGCAAGGAGGAAGTCATTGTCTACGACAATGCCGTTGGCTTCGGCTTCGACTCGCACTTGGTATCTGATGCTTCCAAGATCTGGCAGGATGTACACTATGAATCCCTTGAACCGTTGTATCACATGAAGCATTTTGTACCAAGGGAGTCGCCTTGGAAGCTAGATGGTGAGTCGACAAGAATATCCTCTAAGCTAAAATTACTTGAACAGGAATTACTGAATTTGGAAACTGTTGGAAAGAATGATATTTCTAAGTTACCTTCATTGATGAGGAAGCAGGCCAAGAGGTATCAAGCTCTTGCGGGGAAGATAGATGATCTGTGCAGAAGAATGCAAGGTAGTGATCCCTCCGAACGTACACTCAGTCCAGAGTTTCGTACACAACGACAAACAGAATTTTTACTCGAATCATTTAGACTTCAACAACGTGCGACAGAAACTGGACAGAAGTTGATGGCAGTACAAACGGAGATTGGGAAGAGTTCTTATGGAGATGAGCTGGGGAACCAGCCCAAACACAACACACGACGGTGTTCAGATTCCATTAGAAACAACTTGAAAGAAATCCAAAGAAATTTGGAGATATGGTTGGCCAGAATCATCGGAGATCTTGAAGGTATTCTGGCTAGAGACGGCGCTTCTCGTGTAAGGGAATACTATATCTCTAGCTGTCCTTTTGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

45.95

Weight (kDa)

5.48

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 600
AccI GTMKAC 2 cut(s) 540, 692
AccII CGCG 1 cut(s) 169
AciI CCGC 7 cut(s) 169, 182, 268, 295, 459, 505, 821
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 2 cut(s) 447, 1110
AcsI RAATTY 3 cut(s) 739, 914, 1093
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 5 cut(s) 612, 657, 877, 898, 981
AfiI CCNNNNNNNGG 5 cut(s) 97, 240, 390, 523, 600
AgsI TTSAA 5 cut(s) 629, 725, 941, 1078, 1133
AjnI CCWGG 8 cut(s) 96, 108, 132, 209, 239, 347, 372, 389
AleI CACNNNNGTG 1 cut(s) 1048
Alw21I GWGCWC 2 cut(s) 141, 162
Alw26I GTCTC 1 cut(s) 1143
AlwI GGATC 1 cut(s) 857
AlwNI CAGNNNCTG 1 cut(s) 959
AoxI GGCC 4 cut(s) 88, 447, 798, 1110
ApeKI GCWGC 2 cut(s) 298, 456
ApoI RAATTY 3 cut(s) 739, 914, 1093
Asp700I GAANNNNTTC 2 cut(s) 71, 1137
AspLEI GCGC 2 cut(s) 169, 1157
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 2 cut(s) 494, 698
AvaII GGWCC 1 cut(s) 467
AxyI CCTNAGG 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 18
BaeI ACNNNNGTAYC 2 cut(s) 620, 653
BalI TGGCCA 2 cut(s) 449, 1112
BanII GRGCYC 2 cut(s) 141, 162
BauI CACGAG 1 cut(s) 1161
Bbv12I GWGCWC 2 cut(s) 141, 162
BbvI GCAGC 2 cut(s) 285, 468
BccI CCATC 2 cut(s) 677, 967
BceAI ACGGC 3 cut(s) 502, 536, 1168
BcgI CGANNNNNNTGC 4 cut(s) 146, 180, 368, 402
BciT130I CCWGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
BcoDI GTCTC 1 cut(s) 1143
BfaI CTAG 4 cut(s) 38, 680, 1146, 1185
BfoI RGCGCY 1 cut(s) 1158
BglI GCCNNNNNGGC 1 cut(s) 153
BglII AGATCT 2 cut(s) 597, 1126
BisI GCNGC 4 cut(s) 170, 296, 299, 457
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 4 cut(s) 171, 297, 300, 458
Bme1390I CCNGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 1 cut(s) 1025
BmrFI CCNGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
BmsI GCATC 2 cut(s) 46, 577
BpmI CTGGAG 3 cut(s) 125, 377, 383
Bpu10I CCTNAGC 1 cut(s) 92
Bpu1102I GCTNAGC 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 8 cut(s) 85, 97, 240, 285, 372, 517, 659, 671
BsaXI ACNNNNNCTCC 2 cut(s) 1091, 1121
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 240, 390, 523, 600
Bse1I ACTGG 2 cut(s) 384, 964
Bse21I CCTNAGG 1 cut(s) 471
BseBI CCWGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
BseDI CCNNGG 8 cut(s) 85, 97, 240, 285, 372, 517, 659, 671
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 5 cut(s) 97, 240, 390, 523, 600
BseMII CTCAG 4 cut(s) 83, 462, 468, 895
BseNI ACTGG 2 cut(s) 384, 964
BseRI GAGGAG 3 cut(s) 302, 434, 477
BseSI GKGCMC 1 cut(s) 18
BseXI GCAGC 2 cut(s) 285, 468
BseYI CCCAGC 1 cut(s) 1018
BsgI GTGCAG 1 cut(s) 862
Bsh1236I CGCG 1 cut(s) 169
BshFI GGCC 4 cut(s) 90, 449, 800, 1112
BsiHKAI GWGCWC 2 cut(s) 141, 162
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 5 cut(s) 97, 240, 390, 523, 600
BsmAI GTCTC 1 cut(s) 1143
BsmBI CGTCTC 1 cut(s) 1143
BsmFI GGGAC 1 cut(s) 440
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 4 cut(s) 90, 449, 800, 1112
Bsp1286I GDGCHC 3 cut(s) 18, 141, 162
Bsp1407I TGTACA 1 cut(s) 610
Bsp143I GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
Bsp1720I GCTNAGC 1 cut(s) 477
BspACI CCGC 7 cut(s) 169, 182, 268, 295, 459, 505, 821
BspANI GGCC 4 cut(s) 90, 449, 800, 1112
BspCNI CTCAG 4 cut(s) 84, 463, 469, 894
BspFNI CGCG 1 cut(s) 169
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 1025
BspPI GGATC 1 cut(s) 857
BspQI GCTCTTC 1 cut(s) 180
BsrGI TGTACA 1 cut(s) 610
BsrI ACTGG 2 cut(s) 384, 964
BssECI CCNNGG 8 cut(s) 85, 97, 240, 285, 372, 517, 659, 671
BssMI GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 1161
BssT1I CCWWGG 3 cut(s) 85, 659, 671
Bst2BI CACGAG 1 cut(s) 1161
Bst2UI CCWGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
Bst4CI ACNGT 4 cut(s) 500, 633, 751, 1050
Bst6I CTCTTC 2 cut(s) 180, 992
BstACI GRCGYC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 610
BstC8I GCNNGC 5 cut(s) 158, 180, 262, 282, 798
BstDEI CTNAG 6 cut(s) 92, 471, 477, 708, 771, 881
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 1158
BstHHI GCGC 2 cut(s) 169, 1157
BstKTI GATC 6 cut(s) 255, 312, 600, 838, 865, 1129
BstMAI GTCTC 1 cut(s) 1143
BstMBI GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 153, 166, 175, 281, 676
BstNI CCWGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
BstSCI CCNGG 8 cut(s) 96, 108, 132, 209, 239, 347, 372, 389
BstSLI GKGCMC 1 cut(s) 18
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 285, 468
BstX2I RGATCY 2 cut(s) 597, 1126
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 2 cut(s) 597, 1126
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 4 cut(s) 90, 449, 800, 1112
BtsCI GGATG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 391, 496
Cac8I GCNNGC 5 cut(s) 158, 180, 262, 282, 798
CaiI CAGNNNCTG 1 cut(s) 959
CciI TCATGA 1 cut(s) 201
CfoI GCGC 2 cut(s) 169, 1157
Cfr13I GGNCC 1 cut(s) 467
CseI GACGC 1 cut(s) 326
Csp6I GTAC 5 cut(s) 611, 656, 876, 897, 980
CviAII CATG 5 cut(s) 202, 344, 433, 445, 643
CviQI GTAC 5 cut(s) 611, 656, 876, 897, 980
DdeI CTNAG 6 cut(s) 92, 471, 477, 708, 771, 881
DpnI GATC 6 cut(s) 254, 311, 599, 837, 864, 1128
DpnII GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
EaeI YGGCCR 2 cut(s) 447, 1110
Eam1104I CTCTTC 2 cut(s) 180, 992
EarI CTCTTC 2 cut(s) 180, 992
EciI GGCGGA 1 cut(s) 197
Ecl136II GAGCTC 2 cut(s) 139, 160
Eco130I CCWWGG 3 cut(s) 85, 659, 671
Eco24I GRGCYC 2 cut(s) 141, 162
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 2 cut(s) 139, 160
Eco81I CCTNAGG 1 cut(s) 471
EcoICRI GAGCTC 2 cut(s) 139, 160
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 8 cut(s) 96, 108, 132, 209, 239, 347, 372, 389
EcoT14I CCWWGG 3 cut(s) 85, 659, 671
EcoT38I GRGCYC 2 cut(s) 141, 162
ErhI CCWWGG 3 cut(s) 85, 659, 671
Esp3I CGTCTC 1 cut(s) 1143
FaeI CATG 5 cut(s) 205, 347, 436, 448, 646
FaqI GGGAC 1 cut(s) 440
FatI CATG 5 cut(s) 201, 343, 432, 444, 642
FauI CCCGC 1 cut(s) 814
FblI GTMKAC 2 cut(s) 540, 692
Fnu4HI GCNGC 4 cut(s) 170, 296, 299, 457
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 2 cut(s) 141, 162
Fsp4HI GCNGC 4 cut(s) 170, 296, 299, 457
FspBI CTAG 4 cut(s) 38, 680, 1146, 1185
GlaI GCGC 2 cut(s) 168, 1156
GluI GCNGC 4 cut(s) 170, 296, 299, 457
GsaI CCCAGC 1 cut(s) 1022
GsuI CTGGAG 3 cut(s) 125, 377, 383
HaeII RGCGCY 1 cut(s) 1158
HaeIII GGCC 4 cut(s) 90, 449, 800, 1112
HgaI GACGC 1 cut(s) 326
HhaI GCGC 2 cut(s) 169, 1157
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 5 cut(s) 205, 347, 436, 448, 646
Hin6I GCGC 2 cut(s) 167, 1155
HinP1I GCGC 2 cut(s) 167, 1155
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HphI GGTGA 2 cut(s) 494, 698
Hpy166II GTNNAC 5 cut(s) 541, 613, 693, 878, 899
Hpy188I TCNGA 4 cut(s) 586, 871, 1057, 1124
Hpy188III TCNNGA 8 cut(s) 122, 142, 202, 250, 356, 398, 887, 1130
Hpy8I GTNNAC 5 cut(s) 541, 613, 693, 878, 899
Hpy99I CGWCG 5 cut(s) 251, 260, 317, 320, 1050
HpyAV CCTTC 2 cut(s) 789, 1127
HpyCH4III ACNGT 4 cut(s) 500, 633, 751, 1050
HpyCH4IV ACGT 2 cut(s) 874, 946
HpyCH4V TGCA 2 cut(s) 843, 853
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 166, 175, 281, 676
HpyF3I CTNAG 6 cut(s) 92, 471, 477, 708, 771, 881
HpySE526I ACGT 2 cut(s) 874, 946
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 5 cut(s) 205, 347, 436, 448, 646
HspAI GCGC 2 cut(s) 167, 1155
Kzo9I GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 4 cut(s) 124, 136, 144, 358
Lsp1109I GCAGC 2 cut(s) 285, 468
LweI GCATC 2 cut(s) 46, 577
MaeI CTAG 4 cut(s) 38, 680, 1146, 1185
MaeII ACGT 2 cut(s) 874, 946
MaeIII GTNAC 2 cut(s) 500, 774
MalI GATC 6 cut(s) 254, 311, 599, 837, 864, 1128
MboI GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
MboII GAAGA 9 cut(s) 53, 56, 59, 62, 76, 197, 838, 858, 1009
MflI RGATCY 2 cut(s) 597, 1126
MhlI GDGCHC 3 cut(s) 18, 141, 162
MlsI TGGCCA 2 cut(s) 449, 1112
MluCI AATT 5 cut(s) 716, 731, 739, 914, 1093
MluNI TGGCCA 2 cut(s) 449, 1112
MlyI GAGTC 4 cut(s) 218, 563, 674, 698
MmeI TCCRAC 1 cut(s) 733
Mox20I TGGCCA 2 cut(s) 449, 1112
MroXI GAANNNNTTC 2 cut(s) 71, 1137
MscI TGGCCA 2 cut(s) 449, 1112
MslI CAYNNNNRTG 2 cut(s) 342, 1048
Msp20I TGGCCA 2 cut(s) 449, 1112
MspA1I CMGCKG 1 cut(s) 459
MspR9I CCNGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
Mva1269I GAATGC 1 cut(s) 855
MvaI CCWGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 5 cut(s) 153, 166, 175, 281, 676
NdeII GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
NlaIII CATG 5 cut(s) 205, 347, 436, 448, 646
NlaIV GGNNCC 1 cut(s) 1025
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 1048
PagI TCATGA 1 cut(s) 201
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 549, 901
PctI GAATGC 1 cut(s) 855
PdmI GAANNNNTTC 2 cut(s) 71, 1137
PfeI GAWTC 7 cut(s) 352, 380, 394, 620, 926, 1058, 1116
PflMI CCANNNNNTGG 1 cut(s) 600
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 4 cut(s) 171, 297, 300, 458
PleI GAGTC 4 cut(s) 218, 563, 673, 697
PpsI GAGTC 4 cut(s) 218, 563, 673, 697
PpuMI RGGWCCY 1 cut(s) 467
Psp124BI GAGCTC 2 cut(s) 141, 162
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 8 cut(s) 96, 108, 132, 209, 239, 347, 372, 389
PspFI CCCAGC 1 cut(s) 1018
PspGI CCWGG 8 cut(s) 96, 108, 132, 209, 239, 347, 372, 389
PspN4I GGNNCC 1 cut(s) 1025
PspPI GGNCC 1 cut(s) 467
PspPPI RGGWCCY 1 cut(s) 467
PsrI GAACNNNNNNTAC 2 cut(s) 717, 749
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 2 cut(s) 597, 1126
RsaI GTAC 5 cut(s) 612, 657, 877, 898, 981
RsaNI GTAC 5 cut(s) 611, 656, 876, 897, 980
RseI CAYNNNNRTG 2 cut(s) 342, 1048
SacI GAGCTC 2 cut(s) 141, 162
SalI GTCGAC 1 cut(s) 691
SapI GCTCTTC 1 cut(s) 180
SatI GCNGC 4 cut(s) 170, 296, 299, 457
Sau3AI GATC 6 cut(s) 252, 309, 597, 835, 862, 1126
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 4 cut(s) 218, 563, 674, 698
ScrFI CCNGG 8 cut(s) 98, 110, 134, 211, 241, 349, 374, 391
SduI GDGCHC 3 cut(s) 18, 141, 162
SfaNI GCATC 2 cut(s) 46, 577
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 342, 1048
Sse9I AATT 5 cut(s) 716, 731, 739, 914, 1093
SsiI CCGC 7 cut(s) 169, 182, 268, 295, 459, 505, 821
SspMI CTAG 4 cut(s) 38, 680, 1146, 1185
SstI GAGCTC 2 cut(s) 141, 162
StyD4I CCNGG 8 cut(s) 96, 108, 132, 209, 239, 347, 372, 389
StyI CCWWGG 3 cut(s) 85, 659, 671
TaaI ACNGT 4 cut(s) 500, 633, 751, 1050
TaiI ACGT 2 cut(s) 877, 949
TaqI TCGA 5 cut(s) 249, 312, 567, 692, 924
TasI AATT 5 cut(s) 716, 731, 739, 914, 1093
TatI WGTACW 2 cut(s) 610, 979
TauI GCSGC 2 cut(s) 172, 298
TfiI GAWTC 7 cut(s) 352, 380, 394, 620, 926, 1058, 1116
TscAI CASTG 2 cut(s) 391, 503
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 2 cut(s) 298, 456
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 5 cut(s) 190, 433, 633, 659, 771
TspGWI ACGGA 1 cut(s) 1001
TspRI CASTG 2 cut(s) 391, 503
Van91I CCANNNNNTGG 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 3 cut(s) 739, 914, 1093
XmiI GTMKAC 2 cut(s) 540, 692
XmnI GAANNNNTTC 2 cut(s) 71, 1137
XspI CTAG 4 cut(s) 38, 680, 1146, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.