FvH4_5g27260

Protein of unknown function (DUF1138)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002659
Physical Location & Seq
Forward (+)
18524155 .. 18526615
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27260.t1

Sequence Viewer

Length: 345 bp
ATGTTGTGGCTTCTATGTACATGGTGGTGTCGGTCCGTCTTCGAAGGCAAGAGGCGGAGGCTGGGAACTCTGGGTTGTGGCTATCATGTGCATTTGGGTTCACTAGTAGCGGCAAAGTACATAATAGGATCTGTCCTGGCAAGCTTTGGAGTTGCATATGTTTGTGACACCACTGTTTCTGACAAAAAGATATTTGGAGGTACCACTCCGGGTACTGTTTCTAACAAGGGATGGTGGGAAGAAACTGACAAGAAATTCCAAGCTTGGCCTCGCACTGCAGGACCCCCCGTTGTCATGAACCCCATCAGTTGCCAGAATTTCATTGTCAAATCCCAGTCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1138

Protein Analysis

115

Amino Acids

12.66

Weight (kDa)

9.27

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1138 PF06592 38 - 110 1.4e-42 Protein of unknown function (DUF1138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 2 cut(s) 55, 110
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 2 cut(s) 254, 316
AfaI GTAC 4 cut(s) 19, 119, 202, 214
AhlI ACTAGT 1 cut(s) 103
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 2 cut(s) 144, 263
AluI AGCT 2 cut(s) 144, 263
AlwI GGATC 1 cut(s) 136
AoxI GGCC 1 cut(s) 266
ApoI RAATTY 2 cut(s) 254, 316
Asp718I GGTACC 1 cut(s) 200
AspS9I GGNCC 2 cut(s) 33, 281
AsuC2I CCSGG 1 cut(s) 210
AsuII TTCGAA 1 cut(s) 42
AvaII GGWCC 2 cut(s) 33, 281
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 31
BccI CCATC 2 cut(s) 225, 311
BciT130I CCWGG 1 cut(s) 137
BcnI CCSGG 1 cut(s) 210
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 137, 210
Bme18I GGWCC 2 cut(s) 33, 281
BmgT120I GGNCC 2 cut(s) 33, 281
BmiI GGNNCC 2 cut(s) 202, 283
BmrFI CCNGG 2 cut(s) 137, 210
BmrI ACTGGG 1 cut(s) 328
BmuI ACTGGG 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 334
BseBI CCWGG 1 cut(s) 137
BseGI GGATG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 334
BseYI CCCAGC 1 cut(s) 61
BshFI GGCC 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 209
BsnI GGCC 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 42
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 1 cut(s) 128
BspACI CCGC 2 cut(s) 55, 110
BspANI GGCC 1 cut(s) 268
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 2 cut(s) 202, 283
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 1 cut(s) 136
BspT104I TTCGAA 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 17
BsrI ACTGG 1 cut(s) 334
BssMI GATC 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 175, 217
BstAUI TGTACA 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 142
BstF5I GGATG 1 cut(s) 236
BstKTI GATC 1 cut(s) 131
BstMBI GATC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 2 cut(s) 135, 208
BstSFI CTRYAG 1 cut(s) 276
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 1 cut(s) 268
BtsCI GGATG 1 cut(s) 236
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 171, 273
Cac8I GCNNGC 1 cut(s) 142
CciI TCATGA 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 33, 281
CpoI CGGWCCG 1 cut(s) 33
Csp6I GTAC 4 cut(s) 18, 118, 201, 213
CspI CGGWCCG 1 cut(s) 33
CviAII CATG 3 cut(s) 21, 86, 295
CviJI RGCY 6 cut(s) 10, 61, 81, 144, 263, 268
CviKI_1 RGCY 6 cut(s) 10, 61, 81, 144, 263, 268
CviQI GTAC 4 cut(s) 18, 118, 201, 213
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
EciI GGCGGA 1 cut(s) 70
Eco47I GGWCC 2 cut(s) 33, 281
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 135
FaeI CATG 3 cut(s) 24, 89, 298
FaiI YATR 7 cut(s) 16, 22, 87, 122, 157, 159, 296
FatI CATG 3 cut(s) 20, 85, 294
FauNDI CATATG 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 1 cut(s) 243
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 104
GluI GCNGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 65
HaeIII GGCC 1 cut(s) 268
HapII CCGG 1 cut(s) 209
Hin1II CATG 3 cut(s) 24, 89, 298
HindIII AAGCTT 2 cut(s) 142, 261
HpaII CCGG 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 2 cut(s) 181, 340
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 2 cut(s) 175, 217
HpyCH4V TGCA 3 cut(s) 91, 155, 278
Hsp92II CATG 3 cut(s) 24, 89, 298
KpnI GGTACC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 128
LpnPI CCDG 7 cut(s) 47, 56, 122, 149, 222, 264, 326
MaeI CTAG 1 cut(s) 104
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 130
MboI GATC 1 cut(s) 128
MboII GAAGA 2 cut(s) 31, 251
MflI RGATCY 1 cut(s) 128
MluCI AATT 2 cut(s) 254, 316
MnlI CCTC 4 cut(s) 45, 51, 191, 279
MslI CAYNNNNRTG 1 cut(s) 25
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 137, 210
MvaI CCWGG 1 cut(s) 137
NciI CCSGG 1 cut(s) 210
NdeI CATATG 1 cut(s) 157
NdeII GATC 1 cut(s) 128
NlaIII CATG 3 cut(s) 24, 89, 298
NlaIV GGNNCC 2 cut(s) 202, 283
NmuCI GTSAC 1 cut(s) 164
NspV TTCGAA 1 cut(s) 42
PagI TCATGA 1 cut(s) 294
PkrI GCNGC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 202, 283
PspPI GGNCC 2 cut(s) 33, 281
PspPPI RGGWCCY 1 cut(s) 281
PstI CTGCAG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 128
RsaI GTAC 4 cut(s) 19, 119, 202, 214
RsaNI GTAC 4 cut(s) 18, 118, 201, 213
RseI CAYNNNNRTG 1 cut(s) 25
Rsr2I CGGWCCG 1 cut(s) 33
RsrII CGGWCCG 1 cut(s) 33
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 1 cut(s) 128
Sau96I GGNCC 2 cut(s) 33, 281
ScrFI CCNGG 2 cut(s) 137, 210
SetI ASST 3 cut(s) 146, 202, 265
SfcI CTRYAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 42
SinI GGWCC 2 cut(s) 33, 281
SmiMI CAYNNNNRTG 1 cut(s) 25
SpeI ACTAGT 1 cut(s) 103
Sse9I AATT 2 cut(s) 254, 316
SsiI CCGC 2 cut(s) 55, 110
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 135, 208
TaaI ACNGT 2 cut(s) 175, 217
TaqI TCGA 1 cut(s) 42
TaqII GACCGA 1 cut(s) 21
TasI AATT 2 cut(s) 254, 316
TatI WGTACW 2 cut(s) 17, 117
TauI GCSGC 1 cut(s) 113
TscAI CASTG 2 cut(s) 178, 280
TseFI GTSAC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 2 cut(s) 310, 311
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 178, 280
VpaK11BI GGWCC 2 cut(s) 33, 281
XapI RAATTY 2 cut(s) 254, 316
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.