Rmu_ssc0000460.1_g000022

Protein of unknown function (DUF1138)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000460.1
OG0002659
Physical Location & Seq
Reverse (-)
104142 .. 104461
320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000460.1_g000022.1.cds

Sequence Viewer

Length: 240 bp
atggcgggaaagtacatcataggatctcttgtggcaacctttggagttgcatatgtttgtgacaccattatttctgacagaaagatatttggaggtaccactcccggaactgtttcaaacaagggatggtgggaagaaactgacaagaaattccaggcctggcctcgcactgcaggacctcccgttgtcatgaaccccatcagtcgccagaatttcattgtcaaatcccagtcagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1138

Protein Analysis

79

Amino Acids

8.68

Weight (kDa)

9.3

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 31
AcsI RAATTY 2 cut(s) 149, 211
AfaI GTAC 2 cut(s) 14, 97
AgsI TTSAA 1 cut(s) 117
AjnI CCWGG 2 cut(s) 153, 158
AlwI GGATC 1 cut(s) 31
AoxI GGCC 2 cut(s) 156, 161
ApoI RAATTY 2 cut(s) 149, 211
Asp700I GAANNNNTTC 1 cut(s) 112
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 95
BccI CCATC 2 cut(s) 120, 206
BciT130I CCWGG 2 cut(s) 155, 160
BcnI CCSGG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 171
Bme1390I CCNGG 3 cut(s) 105, 155, 160
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 3 cut(s) 105, 155, 160
BmrI ACTGGG 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 223
BpuMI CCSGG 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 155, 160
BseGI GGATG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 229
BshFI GGCC 2 cut(s) 158, 163
BshNI GGYRCC 1 cut(s) 95
BsiSI CCGG 1 cut(s) 105
BsnI GGCC 2 cut(s) 158, 163
Bsp143I GATC 1 cut(s) 23
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 158, 163
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 95
BsrI ACTGG 1 cut(s) 229
BssMI GATC 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 155, 160
Bst4CI ACNGT 1 cut(s) 112
BstF5I GGATG 1 cut(s) 131
BstKTI GATC 1 cut(s) 26
BstMBI GATC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 155, 160
BstSCI CCNGG 3 cut(s) 103, 153, 158
BstSFI CTRYAG 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
BsuRI GGCC 2 cut(s) 158, 163
BtsCI GGATG 1 cut(s) 131
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 168
CciI TCATGA 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 13, 96
CviAII CATG 1 cut(s) 190
CviJI RGCY 2 cut(s) 158, 163
CviKI_1 RGCY 2 cut(s) 158, 163
CviQI GTAC 2 cut(s) 13, 96
DpnI GATC 1 cut(s) 25
DpnII GATC 1 cut(s) 23
Eco147I AGGCCT 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 153, 158
FaeI CATG 1 cut(s) 193
FaiI YATR 4 cut(s) 20, 52, 54, 191
FatI CATG 1 cut(s) 189
FauNDI CATATG 1 cut(s) 52
FokI GGATG 1 cut(s) 138
HaeIII GGCC 2 cut(s) 158, 163
HapII CCGG 1 cut(s) 105
Hin1II CATG 1 cut(s) 193
HpaII CCGG 1 cut(s) 105
Hpy188I TCNGA 2 cut(s) 76, 235
Hpy188III TCNNGA 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 2 cut(s) 50, 173
Hsp92II CATG 1 cut(s) 193
KpnI GGTACC 1 cut(s) 99
Kzo9I GATC 1 cut(s) 23
LpnPI CCDG 7 cut(s) 118, 140, 145, 159, 167, 172, 221
MaeIII GTNAC 1 cut(s) 59
MalI GATC 1 cut(s) 25
MboI GATC 1 cut(s) 23
MboII GAAGA 1 cut(s) 146
MflI RGATCY 1 cut(s) 23
MluCI AATT 2 cut(s) 149, 211
MnlI CCTC 3 cut(s) 86, 174, 189
MroXI GAANNNNTTC 1 cut(s) 112
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 3 cut(s) 105, 155, 160
MvaI CCWGG 2 cut(s) 155, 160
NciI CCSGG 1 cut(s) 105
NdeI CATATG 1 cut(s) 52
NdeII GATC 1 cut(s) 23
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 59
PagI TCATGA 1 cut(s) 189
PceI AGGCCT 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 112
PfoI TCCNGGA 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 153, 158
PspGI CCWGG 2 cut(s) 153, 158
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 176
PspPPI RGGWCCY 1 cut(s) 176
PstI CTGCAG 1 cut(s) 175
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 2 cut(s) 14, 97
RsaNI GTAC 2 cut(s) 13, 96
Sau3AI GATC 1 cut(s) 23
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 3 cut(s) 105, 155, 160
SetI ASST 3 cut(s) 41, 97, 181
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 176
Sse9I AATT 2 cut(s) 149, 211
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 1 cut(s) 5
StuI AGGCCT 1 cut(s) 158
StyD4I CCNGG 3 cut(s) 103, 153, 158
TaaI ACNGT 1 cut(s) 112
TasI AATT 2 cut(s) 149, 211
TatI WGTACW 1 cut(s) 12
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 2 cut(s) 205, 206
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 176
XapI RAATTY 2 cut(s) 149, 211
XmnI GAANNNNTTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.