MD12G1077000.v1.1

Protein of unknown function (DUF1138)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002659
Physical Location & Seq
Reverse (-)
9378895 .. 9381175
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1077000.v1.1.491

Sequence Viewer

Length: 432 bp
ATGAATACGACCCCCATTGCATTGTCGTCGATCATCAACAAGTGGCGAGACGAAAAAGGTTCAAGAATTCCTGTCGTGGGAAAGTTTATCTCTCATTATCTTGTCAGAAGTGGAGATAAGAAGAAGGTTCAAGAATTCCTGTTGACAGACACCATCGTGCGGTCTCACCGACTCGGAACTCCTCCTGCGATGCCTGCAAAATACATCATTCCTGCTGTTTTGGGATCATTTGCAGTGGCATGGACAGCTGACCATTATTTCGCTGACAAGAAAATATTTGGAGGAAGTACTCCGTCAACTGTTGCAAACAAAGAATGGTGGGAAGAAACTGACAAGAAGTTTCAGGCATGGCCTCGGACTGCCGGTCCACCGGTGGTCATGAATCCCATTAGCCGTCAGAATTTCATTGTCAAGTCCCGCACCGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1138

Protein Analysis

144

Amino Acids

16.07

Weight (kDa)

9.99

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1138 PF06592 66 - 138 7.9e-42 Protein of unknown function (DUF1138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 160, 418
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 3 cut(s) 66, 134, 400
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 2 cut(s) 77, 159
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 63, 131
AhdI GACNNNNNGTC 1 cut(s) 363
AleI CACNNNNGTG 1 cut(s) 155
AluBI AGCT 1 cut(s) 248
AluI AGCT 1 cut(s) 248
Alw26I GTCTC 2 cut(s) 42, 168
AlwI GGATC 1 cut(s) 232
AoxI GGCC 1 cut(s) 350
ApoI RAATTY 3 cut(s) 66, 134, 400
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AsiGI ACCGGT 1 cut(s) 370
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 158
AvaII GGWCC 1 cut(s) 365
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 378
BcgI CGANNNNNNTGC 2 cut(s) 9, 43
BcoDI GTCTC 2 cut(s) 42, 168
BmcAI AGTACT 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 365
BmeRI GACNNNNNGTC 1 cut(s) 363
BmgT120I GGNCC 1 cut(s) 365
BmsI GCATC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 353
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 159
Bse118I RCCGGY 2 cut(s) 362, 370
Bse3DI GCAATG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 77, 159
BseMI GCAATG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 171
BshFI GGCC 1 cut(s) 352
BshTI ACCGGT 1 cut(s) 370
BsiSI CCGG 2 cut(s) 363, 371
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 77, 159
BsmAI GTCTC 2 cut(s) 42, 168
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 1 cut(s) 352
Bso31I GGTCTC 1 cut(s) 168
Bsp143I GATC 2 cut(s) 30, 224
BspACI CCGC 2 cut(s) 160, 418
BspANI GGCC 1 cut(s) 352
BspHI TCATGA 1 cut(s) 378
BspPI GGATC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 15
BsrFI RCCGGY 2 cut(s) 362, 370
BssAI RCCGGY 2 cut(s) 362, 370
BssECI CCNNGG 1 cut(s) 353
BssMI GATC 2 cut(s) 30, 224
Bst4CI ACNGT 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 195
BstKTI GATC 2 cut(s) 33, 227
BstMAI GTCTC 2 cut(s) 42, 168
BstMBI GATC 2 cut(s) 30, 224
BstMWI GCNNNNNNNGC 2 cut(s) 194, 245
BsuRI GGCC 1 cut(s) 352
BtgZI GCGATG 1 cut(s) 203
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 195
CciI TCATGA 1 cut(s) 378
Cfr10I RCCGGY 2 cut(s) 362, 370
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 288
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 3 cut(s) 240, 348, 379
CviJI RGCY 3 cut(s) 248, 352, 393
CviKI_1 RGCY 3 cut(s) 248, 352, 393
CviQI GTAC 1 cut(s) 288
DpnI GATC 2 cut(s) 32, 226
DpnII GATC 2 cut(s) 30, 224
DriI GACNNNNNGTC 1 cut(s) 363
Eam1105I GACNNNNNGTC 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 365
EcoRI GAATTC 2 cut(s) 66, 134
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 3 cut(s) 243, 351, 382
FaiI YATR 3 cut(s) 241, 349, 380
FaqI GGGAC 1 cut(s) 400
FatI CATG 3 cut(s) 239, 347, 378
FauI CCCGC 1 cut(s) 425
HaeIII GGCC 1 cut(s) 352
HapII CCGG 2 cut(s) 363, 371
Hin1II CATG 3 cut(s) 243, 351, 382
HincII GTYRAC 2 cut(s) 144, 297
HindII GTYRAC 2 cut(s) 144, 297
HinfI GANTC 3 cut(s) 171, 382, 425
HpaII CCGG 2 cut(s) 363, 371
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 3 cut(s) 144, 297, 368
Hpy188I TCNGA 4 cut(s) 107, 176, 357, 399
Hpy188III TCNNGA 4 cut(s) 63, 131, 379, 429
Hpy8I GTNNAC 3 cut(s) 144, 297, 368
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 4 cut(s) 20, 197, 233, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 245
Hsp92II CATG 3 cut(s) 243, 351, 382
Kzo9I GATC 2 cut(s) 30, 224
LpnPI CCDG 8 cut(s) 84, 152, 198, 207, 225, 329, 376, 384
LweI GCATC 1 cut(s) 180
MalI GATC 2 cut(s) 32, 226
MboI GATC 2 cut(s) 30, 224
MboII GAAGA 2 cut(s) 133, 335
MluCI AATT 3 cut(s) 66, 134, 400
MlyI GAGTC 2 cut(s) 165, 419
MnlI CCTC 3 cut(s) 192, 275, 363
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 2 cut(s) 363, 371
MwoI GCNNNNNNNGC 2 cut(s) 194, 245
NdeII GATC 2 cut(s) 30, 224
NlaIII CATG 3 cut(s) 243, 351, 382
OliI CACNNNNGTG 1 cut(s) 155
PagI TCATGA 1 cut(s) 378
PfeI GAWTC 1 cut(s) 382
PinAI ACCGGT 1 cut(s) 370
PleI GAGTC 2 cut(s) 165, 419
PpsI GAGTC 2 cut(s) 165, 419
PspPI GGNCC 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
RseI CAYNNNNRTG 1 cut(s) 155
Sau3AI GATC 2 cut(s) 30, 224
Sau96I GGNCC 1 cut(s) 365
ScaI AGTACT 1 cut(s) 289
SchI GAGTC 2 cut(s) 165, 419
SetI ASST 3 cut(s) 61, 129, 250
SfaNI GCATC 1 cut(s) 180
SgrAI CRCCGGYG 1 cut(s) 370
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 3 cut(s) 66, 134, 400
SsiI CCGC 2 cut(s) 160, 418
SspI AATATT 1 cut(s) 276
TaaI ACNGT 1 cut(s) 301
TaqI TCGA 1 cut(s) 29
TasI AATT 3 cut(s) 66, 134, 400
TatI WGTACW 1 cut(s) 287
TfiI GAWTC 1 cut(s) 382
TscAI CASTG 1 cut(s) 240
TspDTI ATGAA 3 cut(s) 17, 394, 395
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 240
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 3 cut(s) 66, 134, 400
ZrmI AGTACT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.