Rorug03G0194400

Protein of unknown function (DUF1138)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002659
Physical Location & Seq
Forward (+)
17110246 .. 17114938
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0194400.1

Sequence Viewer

Length: 3723 bp
ATGGAGTTCTATCAGTATGTCTTTGAGACGATGAGGATGATTATTGTGATTAGTGCGGTAATGGTGCAATGGTTCGAATTCGGTGTCATAGCCTGCCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTGATTGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTTACACGGTTGAAACCCCAGAGCAGATATCAGAGGGTAATCAGGAACTGGTTGACGTCGTTGAAACCCCAGATCCAATATCAGAGGATCATCAAGAACCGGTTGACGTCGACGCCTCTGCATCATCAGCTGAATCACCAGCTCCTACGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTAAAGATACTCGTAAGAGCATCACATTCTCTTTACACAAACGACTGCAAAAAAGGAGAATCAAAACATTCATGGATGACCGAGATCTTGAACGAGGGGATGTTATTTCTGACGCTCTGCTAACGGCAATTAAAAAATCAAGGTTTGCAATTGTTGTTCTCTCTCAAAACTATGCCTCTTCCGCTTGGTGTTTGGAGGAACTGAGACACATTTGTGAATGCATGAAAGGAGACGACAAAAGAATTCTACCACTTTTTTATAATGTGGATCCTACTGATGTAAGACGTCAGAAGAGCAGTTTCGGAGATGCTTTAACTATGCATGAAAATTCTAGGCGACACGGAGAGAAGGTGAAGCAGTGGAGAGATGCTGTAACAAAGGTGGCAAATTTCTCCGGGTGGCATTCAGAGACATTTAACAATGACGAAGAACTTTTGGTTAAAATTGTGGAAGAGGTGTGCAATAAATTACGACCGACTGAACCTGTGTTAAAAGTGTCCCTCGGAGATTTTGAAGAATTTGCAGCAACAAAAGAAGCCATTGATAAGGTCATGGAGGCGCTAAAAGATGGTGAGGCCACTACCATTGGAGTCTACGGCATGGGGGGCGTTGGAAAGACAACAATGGTGAAACATGTAGGTGTACAAGCCGAAGAAAAAAAGCTTTTTAATAAAGTGATTATGGCTGTCGTATCCCAAAAACCCGACTTGAAGAAAATTCAACAGGAATTTGCAGAAACGCTGGGCTTTGAACTCAAGGAGAAGACGGAAATTGTAAGAGCCCGTGAATTGAAGGAGAAAATAATGAGAGAAACCGGGATCCTCATAATCTTGGATGACATTTGGCAGAGAATAAAGTTTTCAGACATTGGAATTCCGAGCCACAACGAACTTCAAACACGCAATTCCAAAGTCCTAATGACCACCAGAAGATTGAAAGTTTGTAAGATGATGGGGACCCATGCAAAAATTCCTCTCAATACCTTATCAGAAGAAGATTCTTGGCGCTTATTTGTGAGGGAAGCGGGGATGTCTTCTGACAAATCTTCCAATTTCTATGATGTAGCACATGAGGTGGCAAGAGAATGTGCCGGTCTACCAATTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGAACAGATGGGAAGAAGCTGCAGGACGACTAAAAGCGTCTCAACCTCCCATCCGTGAAGATGAGAAAGCTGTGTTCAAATGCATAAAGTTAAGCTATGATTTCTTGAAGGATGATGAATTGAAATCATGCTTCTTGCTTTGCTGCTTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGGTCATGTATGGAATTGGGAAAGGAATGTTTCGAGATTTAACCATGCTACAAGCCCGAGAGTCAACATATTCAGTGGTGCAGGACCTTGAAGATTCTAGCTTGCTTTTAGGCGGTAGAGATGACGGATGTGTAAGGATGCATGATGTCATTCGGGATATGGCAATATTAATGACATCATTATCTGAAGACGGCCAGCGGTTCTTGGTGAAAGTTGGCTGTGAATTGACGAATTGGCCAGAGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCGAGCTACCAGAAAAGTTGGTATGTCCAAATCTCCAGATTTTATCACTACGACAGAATGCTTCTTTAAATGATATCCCAAAATCTTTTTTCCAAAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCAACACTCGTATTTCATTACTACCCCAATCAATCGTTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCAGAAACATAATGGACACTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGAAAAAATTGTCAAGAGAAATAGAACAACTGACCAATCTAAGGATGCTCGATATAAGTGCTGCAGCTTTATCCTTGGGAGGAAGTATTTGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAGCTGTACATGCAATATGGATTTTGGGACTGGGGGAGTAAAATTGATGGAAAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTAAGAATTTTGAAAGTTTGCATATTAGATGCAAATTGCATCCCTAAAAGTGTTAAGGTCGAACCGAAGTGGGTTTACTTTGATATTAGTATTTGCAGCGGCAAGAGTGGACAGGGAATCTATAACATCCTTCGATCTGGTAATAATTATAGATCCTTGAGTCTTGATAGTACTGACACAACCATGGGAACCCTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAAAACTGAGAGGCTGAGTTATAAAGAATGCACAGGGTTGAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTGCATGGACTGAAAGTTCTCTCTGTAATTGACTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATATGCGTATGCCTGTGTTTGAGAACATGGAAGAGTTGCATCTGAAAGAGCTGAATGACCTGGAGGAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCCATCAGTGTCGTAACTTGGAGAAAGTACTGTTGCCATCAAATTTGTTGCAGAAACTACCAAATTTGGAAATACTAAACTGTGAGGAAAGTAAAGTGGATCATGTGTTTGAATGTAAAGGATTTGAGCCAGATCAAACAAAACTGAGAGAGATGGAGTTGCGAGATCTAGATTTAATAAGAAGCATATGTAATGGTCCTGCTCCACGCCGAATGTTCCAGGCTCTAAAGCAATTGGTAATTCACAATTGCAACTTCCAAGGAAGTCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAAGCCTTGTTGTATCCGAATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAAGAGGAAAACATTGAACAACAACAAGACCGTTCTTCCAAAATTGAAGAACTTATGTTTGCAATATCTTCCAATGTTGTACAAGGGAAGTGCTACTGTTGATTTTGTGTGTCCTTCATTGGAAACCTTGCATCTGAGGGACTGCCCCCACTTGTCATTTCCACCCTCCTCTGCTTCTGACTACTTCCACAGCAGGAACCCAGTCAATGAAGATTGGAGTCTGTTTCTCCGATTTAAAGTAAAGGCTTCAGTATTTCCAAGAAGAAACATGAAGAAAGCAAATCTGAGTGAGAGGTCGCGGCCTCGCTCCAGAGGGAGAGAACACATGAAGAAAGCAAATCTGAGTGATAGGGCGCGGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1138

Protein Analysis

1240

Amino Acids

141.58

Weight (kDa)

6.91

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 115 - 285 1.6e-52 TIR domain
TIR_2 PF13676 118 - 214 2.4e-16 TIR domain
NB-ARC PF00931 301 - 469 6.9e-30 NB-ARC domain
LRR_8 PF13855 673 - 732 4e-10 Leucine rich repeat
LRR_14 PF23598 675 - 810 1.5e-14 Leucine-rich repeat region
LRR_RPS2 PF23247 969 - 1110 3e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1049 - 1179 6.7e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 2748
AasI GACNNNNNNGTC 1 cut(s) 2708
AatII GACGTC 3 cut(s) 249, 300, 658
AccI GTMKAC 3 cut(s) 300, 963, 1465
AccII CGCG 2 cut(s) 3655, 3712
AccIII TCCGGA 1 cut(s) 2698
AciI CCGC 8 cut(s) 56, 552, 1394, 1809, 1894, 2601, 3655, 3712
AcoI YGGCCR 2 cut(s) 1888, 1931
AcuI CTGAAG 3 cut(s) 349, 1902, 3588
AcyI GRCGYC 4 cut(s) 246, 297, 303, 655
AfaI GTAC 5 cut(s) 1014, 2408, 2674, 3030, 3437
AfiI CCNNNNNNNGG 3 cut(s) 165, 2307, 3337
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 2 cut(s) 1003, 2867
AgeI ACCGGT 1 cut(s) 289
AjnI CCWGG 3 cut(s) 2937, 2968, 3219
AjuI GAANNNNNNNTTGG 2 cut(s) 2454, 2486
AleI CACNNNNGTG 1 cut(s) 582
AloI GAACNNNNNNTCC 2 cut(s) 2799, 2831
Alw26I GTCTC 6 cut(s) 20, 568, 594, 773, 1557, 3272
AlwNI CAGNNNCTG 1 cut(s) 238
Ama87I CYCGRG 1 cut(s) 1752
Aor13HI TCCGGA 1 cut(s) 2698
AoxI GGCC 6 cut(s) 945, 1494, 1888, 1931, 3656, 3713
ApaI GGGCCC 1 cut(s) 1498
ApeKI GCWGC 6 cut(s) 893, 1532, 1656, 2327, 2330, 2598
ArsI GACNNNNNNTTYG 4 cut(s) 69, 101, 3318, 3350
AseI ATTAAT 1 cut(s) 1865
AsiGI ACCGGT 1 cut(s) 289
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 3 cut(s) 931, 1377, 3712
AspS9I GGNCC 6 cut(s) 1326, 1494, 1495, 1780, 3001, 3197
AsuC2I CCSGG 2 cut(s) 766, 1186
AsuHPI GGTGA 7 cut(s) 145, 318, 733, 953, 1009, 1915, 2969
AsuII TTCGAA 1 cut(s) 75
AvaI CYCGRG 1 cut(s) 1752
AvaII GGWCC 4 cut(s) 1326, 1780, 3001, 3197
BaeGI GKGCMC 1 cut(s) 1498
BalI TGGCCA 1 cut(s) 1933
BamHI GGATCC 2 cut(s) 637, 1188
BanII GRGCYC 2 cut(s) 1153, 1498
BarI GAAGNNNNNNTAC 2 cut(s) 2561, 2593
BbsI GAAGAC 3 cut(s) 1139, 1395, 1890
BbvI GCAGC 6 cut(s) 905, 1519, 1643, 2314, 2342, 2610
BccI CCATC 9 cut(s) 932, 1315, 1515, 1571, 2374, 2441, 3012, 3046, 3148
BceAI ACGGC 3 cut(s) 510, 982, 1903
BcgI CGANNNNNNTGC 4 cut(s) 290, 324, 2306, 2340
BciT130I CCWGG 3 cut(s) 2939, 2970, 3221
BciVI GTATCC 2 cut(s) 1072, 3331
BcnI CCSGG 2 cut(s) 766, 1186
BcoDI GTCTC 6 cut(s) 20, 568, 594, 773, 1557, 3272
BfaI CTAG 3 cut(s) 702, 1794, 3170
BfmI CTRYAG 3 cut(s) 1533, 2328, 2980
BfoI RGCGCY 2 cut(s) 932, 1378
BfrI CTTAAG 1 cut(s) 98
BfuI GTATCC 2 cut(s) 1072, 3331
BglII AGATCT 2 cut(s) 454, 3166
BisI GCNGC 9 cut(s) 894, 1533, 1657, 2328, 2331, 2599, 2602, 3656, 3713
BlsI GCNGC 9 cut(s) 895, 1534, 1658, 2329, 2332, 2600, 2603, 3657, 3714
BmcAI AGTACT 2 cut(s) 2674, 3030
Bme1390I CCNGG 5 cut(s) 766, 1186, 2939, 2970, 3221
Bme18I GGWCC 4 cut(s) 1326, 1780, 3001, 3197
BmeT110I CYCGRG 1 cut(s) 1752
BmgT120I GGNCC 6 cut(s) 1326, 1494, 1495, 1780, 3001, 3197
BmiI GGNNCC 7 cut(s) 639, 1190, 1327, 1328, 1496, 2692, 3554
BmrFI CCNGG 5 cut(s) 766, 1186, 2939, 2970, 3221
BmrI ACTGGG 2 cut(s) 2440, 3551
BmuI ACTGGG 2 cut(s) 2440, 3551
BpiI GAAGAC 3 cut(s) 1139, 1395, 1890
BpmI CTGGAG 3 cut(s) 2009, 2960, 3649
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 3 cut(s) 1109, 2258, 2680
BpuMI CCSGG 2 cut(s) 766, 1186
BsaAI YACGTR 1 cut(s) 339
BsaHI GRCGYC 4 cut(s) 246, 297, 303, 655
BsaJI CCNNGG 5 cut(s) 871, 2340, 2685, 2791, 3259
BsaWI WCCGGW 2 cut(s) 289, 2698
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 2307, 3337
Bse118I RCCGGY 2 cut(s) 289, 1460
Bse1I ACTGG 5 cut(s) 159, 243, 2435, 2708, 3557
Bse3DI GCAATG 1 cut(s) 74
BseAI TCCGGA 1 cut(s) 2698
BseBI CCWGG 3 cut(s) 2939, 2970, 3221
BseDI CCNNGG 5 cut(s) 871, 2340, 2685, 2791, 3259
BseLI CCNNNNNNNGG 3 cut(s) 165, 2307, 3337
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 8 cut(s) 563, 2724, 2732, 3137, 3302, 3482, 3632, 3689
BseNI ACTGG 5 cut(s) 159, 243, 2435, 2708, 3557
BseRI GAGGAG 2 cut(s) 2957, 3514
BseSI GKGCMC 1 cut(s) 1498
BseXI GCAGC 6 cut(s) 905, 1519, 1643, 2314, 2342, 2610
BseYI CCCAGC 1 cut(s) 1111
BsgI GTGCAG 1 cut(s) 1796
Bsh1236I CGCG 2 cut(s) 3655, 3712
Bsh1285I CGRYCG 1 cut(s) 845
BshFI GGCC 6 cut(s) 947, 1496, 1890, 1933, 3658, 3715
BshTI ACCGGT 1 cut(s) 289
BsiEI CGRYCG 1 cut(s) 845
BsiHKCI CYCGRG 1 cut(s) 1752
BsiSI CCGG 5 cut(s) 290, 765, 1185, 1461, 2699
BslFI GGGAC 4 cut(s) 853, 1339, 2441, 3509
BslI CCNNNNNNNGG 3 cut(s) 165, 2307, 3337
BsmAI GTCTC 6 cut(s) 20, 568, 594, 773, 1557, 3272
BsmBI CGTCTC 3 cut(s) 20, 594, 1557
BsmFI GGGAC 4 cut(s) 853, 1339, 2441, 3509
BsmI GAATGC 6 cut(s) 593, 772, 2053, 2759, 2863, 3332
BsnI GGCC 6 cut(s) 947, 1496, 1890, 1933, 3658, 3715
BsoBI CYCGRG 1 cut(s) 1752
Bsp119I TTCGAA 1 cut(s) 75
Bsp120I GGGCCC 1 cut(s) 1494
Bsp1286I GDGCHC 2 cut(s) 1153, 1498
Bsp13I TCCGGA 1 cut(s) 2698
Bsp1407I TGTACA 3 cut(s) 1012, 2406, 3435
Bsp19I CCATGG 1 cut(s) 2685
BspACI CCGC 8 cut(s) 56, 552, 1394, 1809, 1894, 2601, 3655, 3712
BspANI GGCC 6 cut(s) 947, 1496, 1890, 1933, 3658, 3715
BspCNI CTCAG 8 cut(s) 564, 2725, 2733, 3138, 3301, 3483, 3633, 3690
BspEI TCCGGA 1 cut(s) 2698
BspFNI CGCG 2 cut(s) 3655, 3712
BspLI GGNNCC 7 cut(s) 639, 1190, 1327, 1328, 1496, 2692, 3554
BspMAI CTGCAG 3 cut(s) 1537, 2332, 2984
BspQI GCTCTTC 2 cut(s) 656, 2394
BspT104I TTCGAA 1 cut(s) 75
BspTI CTTAAG 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 2 cut(s) 289, 1460
BsrGI TGTACA 3 cut(s) 1012, 2406, 3435
BsrI ACTGG 5 cut(s) 159, 243, 2435, 2708, 3557
BssAI RCCGGY 2 cut(s) 289, 1460
BssECI CCNNGG 5 cut(s) 871, 2340, 2685, 2791, 3259
BssNI GRCGYC 4 cut(s) 246, 297, 303, 655
BssT1I CCWWGG 3 cut(s) 2340, 2685, 3259
Bst2UI CCWGG 3 cut(s) 2939, 2970, 3221
Bst4CI ACNGT 7 cut(s) 199, 2792, 2834, 3033, 3083, 3388, 3454
Bst6I CTCTTC 7 cut(s) 173, 553, 656, 816, 2394, 2904, 3275
BstACI GRCGYC 4 cut(s) 246, 297, 303, 655
BstAFI CTTAAG 1 cut(s) 98
BstAUI TGTACA 3 cut(s) 1012, 2406, 3435
BstBAI YACGTR 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 4 cut(s) 94, 1799, 1892, 1994
BstDSI CCRYGG 2 cut(s) 2685, 2791
BstFNI CGCG 2 cut(s) 3655, 3712
BstH2I RGCGCY 2 cut(s) 932, 1378
BstHHI GCGC 3 cut(s) 931, 1377, 3712
BstMAI GTCTC 6 cut(s) 20, 568, 594, 773, 1557, 3272
BstMCI CGRYCG 1 cut(s) 845
BstMWI GCNNNNNNNGC 5 cut(s) 317, 551, 975, 1481, 2410
BstNI CCWGG 3 cut(s) 2939, 2970, 3221
BstNSI RCATGY 3 cut(s) 1007, 2413, 2871
BstSCI CCNGG 5 cut(s) 764, 1184, 2937, 2968, 3219
BstSFI CTRYAG 3 cut(s) 1533, 2328, 2980
BstSLI GKGCMC 1 cut(s) 1498
BstUI CGCG 2 cut(s) 3655, 3712
BstV1I GCAGC 6 cut(s) 905, 1519, 1643, 2314, 2342, 2610
BstV2I GAAGAC 3 cut(s) 1139, 1395, 1890
BstX2I RGATCY 8 cut(s) 262, 454, 637, 1188, 2110, 2654, 2972, 3166
BstYI RGATCY 8 cut(s) 262, 454, 637, 1188, 2110, 2654, 2972, 3166
BsuI GTATCC 2 cut(s) 1072, 3331
BsuRI GGCC 6 cut(s) 947, 1496, 1890, 1933, 3658, 3715
BtgI CCRYGG 2 cut(s) 2685, 2791
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 4 cut(s) 734, 1776, 3014, 3296
Cac8I GCNNGC 4 cut(s) 94, 1799, 1892, 1994
CaiI CAGNNNCTG 1 cut(s) 238
CfoI GCGC 3 cut(s) 931, 1377, 3712
Cfr10I RCCGGY 2 cut(s) 289, 1460
Cfr13I GGNCC 6 cut(s) 1326, 1494, 1495, 1780, 3001, 3197
CseI GACGC 3 cut(s) 311, 491, 1539
Csp6I GTAC 5 cut(s) 1013, 2407, 2673, 3029, 3436
CspAI ACCGGT 1 cut(s) 289
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CviQI GTAC 5 cut(s) 1013, 2407, 2673, 3029, 3436
DraI TTTAAA 2 cut(s) 2058, 3592
DrdI GACNNNNNNGTC 1 cut(s) 2708
DseDI GACNNNNNNGTC 1 cut(s) 2708
EaeI YGGCCR 2 cut(s) 1888, 1931
Eam1104I CTCTTC 7 cut(s) 173, 553, 656, 816, 2394, 2904, 3275
EarI CTCTTC 7 cut(s) 173, 553, 656, 816, 2394, 2904, 3275
Eco130I CCWWGG 3 cut(s) 2340, 2685, 3259
Eco24I GRGCYC 2 cut(s) 1153, 1498
Eco32I GATATC 4 cut(s) 219, 1681, 2065, 2379
Eco47I GGWCC 4 cut(s) 1326, 1780, 3001, 3197
Eco57I CTGAAG 3 cut(s) 349, 1902, 3588
Eco88I CYCGRG 1 cut(s) 1752
EcoO109I RGGNCCY 4 cut(s) 1326, 1494, 1495, 1780
EcoRI GAATTC 3 cut(s) 77, 612, 1242
EcoRII CCWGG 3 cut(s) 2937, 2968, 3219
EcoRV GATATC 4 cut(s) 219, 1681, 2065, 2379
EcoT14I CCWWGG 3 cut(s) 2340, 2685, 3259
EcoT22I ATGCAT 4 cut(s) 593, 693, 1598, 1839
EcoT38I GRGCYC 2 cut(s) 1153, 1498
ErhI CCWWGG 3 cut(s) 2340, 2685, 3259
Esp3I CGTCTC 3 cut(s) 20, 594, 1557
FaqI GGGAC 4 cut(s) 853, 1339, 2441, 3509
FauI CCCGC 1 cut(s) 1387
FauNDI CATATG 1 cut(s) 3188
FblI GTMKAC 3 cut(s) 300, 963, 1465
Fnu4HI GCNGC 9 cut(s) 894, 1533, 1657, 2328, 2331, 2599, 2602, 3656, 3713
FriOI GRGCYC 2 cut(s) 1153, 1498
Fsp4HI GCNGC 9 cut(s) 894, 1533, 1657, 2328, 2331, 2599, 2602, 3656, 3713
FspBI CTAG 3 cut(s) 702, 1794, 3170
GlaI GCGC 3 cut(s) 930, 1376, 3711
GluI GCNGC 9 cut(s) 894, 1533, 1657, 2328, 2331, 2599, 2602, 3656, 3713
GsaI CCCAGC 1 cut(s) 1115
GsuI CTGGAG 3 cut(s) 2009, 2960, 3649
HaeII RGCGCY 2 cut(s) 932, 1378
HaeIII GGCC 6 cut(s) 947, 1496, 1890, 1933, 3658, 3715
HapII CCGG 5 cut(s) 290, 765, 1185, 1461, 2699
HgaI GACGC 3 cut(s) 311, 491, 1539
HhaI GCGC 3 cut(s) 931, 1377, 3712
Hin1I GRCGYC 4 cut(s) 246, 297, 303, 655
Hin6I GCGC 3 cut(s) 929, 1375, 3710
HinP1I GCGC 3 cut(s) 929, 1375, 3710
HincII GTYRAC 5 cut(s) 244, 295, 301, 1761, 2713
HindII GTYRAC 5 cut(s) 244, 295, 301, 1761, 2713
HindIII AAGCTT 3 cut(s) 1031, 2172, 2234
HpaII CCGG 5 cut(s) 290, 765, 1185, 1461, 2699
HphI GGTGA 7 cut(s) 145, 318, 733, 953, 1009, 1915, 2969
Hpy99I CGWCG 3 cut(s) 251, 302, 305
HpyCH4III ACNGT 7 cut(s) 199, 2792, 2834, 3033, 3083, 3388, 3454
HpyCH4IV ACGT 5 cut(s) 150, 246, 297, 338, 655
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 551, 975, 1481, 2410
HpySE526I ACGT 5 cut(s) 150, 246, 297, 338, 655
Hsp92I GRCGYC 4 cut(s) 246, 297, 303, 655
HspAI GCGC 3 cut(s) 929, 1375, 3710
KflI GGGWCCC 1 cut(s) 1326
Kpn2I TCCGGA 1 cut(s) 2698
LguI GCTCTTC 2 cut(s) 656, 2394
LmnI GCTCC 3 cut(s) 337, 3208, 3668
Lsp1109I GCAGC 6 cut(s) 905, 1519, 1643, 2314, 2342, 2610
MaeI CTAG 3 cut(s) 702, 1794, 3170
MaeII ACGT 5 cut(s) 150, 246, 297, 338, 655
MaeIII GTNAC 6 cut(s) 139, 191, 742, 2768, 2961, 3014
MfeI CAATTG 6 cut(s) 519, 1470, 1686, 3233, 3247, 3302
MflI RGATCY 8 cut(s) 262, 454, 637, 1188, 2110, 2654, 2972, 3166
MhlI GDGCHC 2 cut(s) 1153, 1498
MlsI TGGCCA 1 cut(s) 1933
MluNI TGGCCA 1 cut(s) 1933
MlyI GAGTC 5 cut(s) 969, 1766, 2112, 2671, 3583
MmeI TCCRAC 1 cut(s) 961
Mox20I TGGCCA 1 cut(s) 1933
Mph1103I ATGCAT 4 cut(s) 593, 693, 1598, 1839
MroI TCCGGA 1 cut(s) 2698
MroXI GAANNNNTTC 1 cut(s) 119
MscI TGGCCA 1 cut(s) 1933
MslI CAYNNNNRTG 5 cut(s) 128, 582, 1008, 2684, 2777
Msp20I TGGCCA 1 cut(s) 1933
MspA1I CMGCKG 3 cut(s) 320, 1894, 2601
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 5 cut(s) 290, 765, 1185, 1461, 2699
MspR9I CCNGG 5 cut(s) 766, 1186, 2939, 2970, 3221
MunI CAATTG 6 cut(s) 519, 1470, 1686, 3233, 3247, 3302
Mva1269I GAATGC 6 cut(s) 593, 772, 2053, 2759, 2863, 3332
MvaI CCWGG 3 cut(s) 2939, 2970, 3221
MvnI CGCG 2 cut(s) 3655, 3712
MwoI GCNNNNNNNGC 5 cut(s) 317, 551, 975, 1481, 2410
NciI CCSGG 2 cut(s) 766, 1186
NcoI CCATGG 1 cut(s) 2685
NdeI CATATG 1 cut(s) 3188
NlaIV GGNNCC 7 cut(s) 639, 1190, 1327, 1328, 1496, 2692, 3554
NmuCI GTSAC 1 cut(s) 139
NsiI ATGCAT 4 cut(s) 593, 693, 1598, 1839
NspI RCATGY 3 cut(s) 1007, 2413, 2871
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 1 cut(s) 582
PciI ACATGT 2 cut(s) 1003, 2867
PciSI GCTCTTC 2 cut(s) 656, 2394
PctI GAATGC 6 cut(s) 593, 772, 2053, 2759, 2863, 3332
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 6 cut(s) 323, 429, 1367, 1790, 2242, 2619
PinAI ACCGGT 1 cut(s) 289
PkrI GCNGC 9 cut(s) 895, 1534, 1658, 2329, 2332, 2600, 2603, 3657, 3714
PleI GAGTC 5 cut(s) 968, 1765, 2111, 2670, 3582
PpsI GAGTC 5 cut(s) 968, 1765, 2111, 2670, 3582
Ppu21I YACGTR 1 cut(s) 339
PpuMI RGGWCCY 2 cut(s) 1326, 1780
PscI ACATGT 2 cut(s) 1003, 2867
PshBI ATTAAT 1 cut(s) 1865
PsiI TTATAA 2 cut(s) 630, 2748
Psp5II RGGWCCY 2 cut(s) 1326, 1780
Psp6I CCWGG 3 cut(s) 2937, 2968, 3219
PspFI CCCAGC 1 cut(s) 1111
PspGI CCWGG 3 cut(s) 2937, 2968, 3219
PspN4I GGNNCC 7 cut(s) 639, 1190, 1327, 1328, 1496, 2692, 3554
PspOMI GGGCCC 1 cut(s) 1494
PspPI GGNCC 6 cut(s) 1326, 1494, 1495, 1780, 3001, 3197
PspPPI RGGWCCY 2 cut(s) 1326, 1780
PstI CTGCAG 3 cut(s) 1537, 2332, 2984
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 8 cut(s) 262, 454, 637, 1188, 2110, 2654, 2972, 3166
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 5 cut(s) 1014, 2408, 2674, 3030, 3437
RsaNI GTAC 5 cut(s) 1013, 2407, 2673, 3029, 3436
RseI CAYNNNNRTG 5 cut(s) 128, 582, 1008, 2684, 2777
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 2 cut(s) 656, 2394
SatI GCNGC 9 cut(s) 894, 1533, 1657, 2328, 2331, 2599, 2602, 3656, 3713
Sau96I GGNCC 6 cut(s) 1326, 1494, 1495, 1780, 3001, 3197
ScaI AGTACT 2 cut(s) 2674, 3030
SchI GAGTC 5 cut(s) 969, 1766, 2112, 2671, 3583
ScrFI CCNGG 5 cut(s) 766, 1186, 2939, 2970, 3221
SduI GDGCHC 2 cut(s) 1153, 1498
SfcI CTRYAG 3 cut(s) 1533, 2328, 2980
SfuI TTCGAA 1 cut(s) 75
SgrDI CGTCGACG 1 cut(s) 299
SinI GGWCC 4 cut(s) 1326, 1780, 3001, 3197
SmiMI CAYNNNNRTG 5 cut(s) 128, 582, 1008, 2684, 2777
SmlI CTYRAG 4 cut(s) 98, 1124, 2237, 2659
SmoI CTYRAG 4 cut(s) 98, 1124, 2237, 2659
SsiI CCGC 8 cut(s) 56, 552, 1394, 1809, 1894, 2601, 3655, 3712
SspI AATATT 2 cut(s) 1863, 2470
SspMI CTAG 3 cut(s) 702, 1794, 3170
StyD4I CCNGG 5 cut(s) 764, 1184, 2937, 2968, 3219
StyI CCWWGG 3 cut(s) 2340, 2685, 3259
TaaI ACNGT 7 cut(s) 199, 2792, 2834, 3033, 3083, 3388, 3454
TaiI ACGT 5 cut(s) 153, 249, 300, 341, 658
TaqI TCGA 8 cut(s) 75, 300, 1501, 1729, 2316, 2562, 2635, 3351
TaqII GACCGA 2 cut(s) 465, 859
TatI WGTACW 5 cut(s) 1012, 2406, 2672, 3028, 3435
TauI GCSGC 3 cut(s) 2604, 3658, 3715
TfiI GAWTC 6 cut(s) 323, 429, 1367, 1790, 2242, 2619
TscAI CASTG 4 cut(s) 734, 1776, 3014, 3296
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 6 cut(s) 893, 1532, 1656, 2327, 2330, 2598
Tsp45I GTSAC 1 cut(s) 139
TspGWI ACGGA 4 cut(s) 726, 1151, 1556, 1836
TspRI CASTG 4 cut(s) 734, 1776, 3014, 3296
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 4 cut(s) 1326, 1780, 3001, 3197
VspI ATTAAT 1 cut(s) 1865
XbaI TCTAGA 1 cut(s) 3169
XceI RCATGY 3 cut(s) 1007, 2413, 2871
XcmI CCANNNNNNNNNTGG 1 cut(s) 336
XmiI GTMKAC 3 cut(s) 300, 963, 1465
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 3 cut(s) 702, 1794, 3170
ZraI GACGTC 3 cut(s) 247, 298, 656
ZrmI AGTACT 2 cut(s) 2674, 3030
Zsp2I ATGCAT 4 cut(s) 593, 693, 1598, 1839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.