FvH4_5g29810

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007408
Physical Location & Seq
Reverse (-)
20696417 .. 20700028
3612 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29810.t3

Sequence Viewer

Length: 861 bp
ATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAACAGCGCGAGATTACGGAACGCCTACGAGACCCTAGGGGACTTCGCAAGGGAGGAGGTGGAGGAGGCTCGGCCGCTGCCGCTCCCCGCAACTTCGCCGCCAATGGCGCTCGCCCGCGAGGCTTCACTCGACCTGCAGATAGGGCTGATGTGGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTGGAGGATGGAGAGATAGTTGAGAGTGCTGAAGGAGCCAAGGATGCGAAGAAGAAGGATTCAAGCGAGGCGGTGAAAGCTGATCGCAGTGATAGGAGGTTGTCAAATTTGCAACAAGGTAGCTGGTTAAGGAGGGATGGCAATCATGGTGCAGCCAAGAAGGACTTTGACATTCCGGCAACGGAGCATGTTCCGAGGGTATTGCCAAAGCAAGAAGACCCCAGCGTGGTTAACAGGAACAAAAGAATGCTCGGGCATGTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAGATATGCAACTCTCAGGAACAGAGGCTTTCATGCGGAGATCAAACTCTTTACAAAGAGCTGAGCAAAGAGCACGTGAAGAAAGTGAAAGACTTCGGATACAAGAGCGAGAACATATTGCGGAAAAGCGGAGAAGAGATCTGACTCTGCGAGCACGTGTTGCTGCCAAGACTGAAGAAAAGAAATTGGAACTGCTTTTTCTTCAGTGGAGTGATCATCATAAGAAACTTAGCAATTTCATAAGGTTATTTTTTATTTTGGTCCAAAAAAGTGTCCATATTCACATTTTATTGAGACGAAGATAA

Protein Analysis

287

Amino Acids

32.72

Weight (kDa)

10.37

Isoelectric Point (pI)

72.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 166 - 294 2.9e-28 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 208
AccBSI CCGCTC 1 cut(s) 149
AccII CGCG 2 cut(s) 75, 184
AclWI GGATC 1 cut(s) 231
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 2 cut(s) 373, 545
AcuI CTGAAG 3 cut(s) 318, 743, 750
AcvI CACGTG 2 cut(s) 632, 713
AfiI CCNNNNNNNGG 1 cut(s) 493
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 1 cut(s) 330
AluBI AGCT 5 cut(s) 58, 257, 347, 390, 617
AluI AGCT 5 cut(s) 58, 257, 347, 390, 617
Alw21I GWGCWC 4 cut(s) 39, 60, 631, 712
Alw26I GTCTC 2 cut(s) 90, 844
AlwI GGATC 1 cut(s) 231
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 3 cut(s) 143, 419, 719
ApoI RAATTY 2 cut(s) 373, 545
Asp700I GAANNNNTTC 1 cut(s) 648
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 3 cut(s) 11, 75, 176
AspS9I GGNCC 1 cut(s) 817
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 817
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 60
BbrPI CACGTG 2 cut(s) 632, 713
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 4 cut(s) 39, 60, 631, 712
BbvI GCAGC 3 cut(s) 130, 431, 706
BccI CCATC 2 cut(s) 269, 398
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 451, 485
BciVI GTATCC 1 cut(s) 648
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 2 cut(s) 90, 844
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 2 cut(s) 12, 177
BfuAI ACCTGC 1 cut(s) 208
BfuI GTATCC 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 186
BglII AGATCT 1 cut(s) 694
BisI GCNGC 7 cut(s) 12, 141, 144, 147, 165, 420, 720
BlnI CCTAGG 1 cut(s) 101
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 7 cut(s) 13, 142, 145, 148, 166, 421, 721
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 1 cut(s) 304
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 618
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 2 cut(s) 632, 713
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 101, 306, 461
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 243
Bse8I GATNNNNATC 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 101, 306, 461
BseGI GGATG 3 cut(s) 280, 316, 409
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMII CTCAG 2 cut(s) 585, 609
BseRI GAGGAG 3 cut(s) 44, 135, 144
BseSI GKGCMC 1 cut(s) 536
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 3 cut(s) 130, 431, 706
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 438
Bsh1236I CGCG 2 cut(s) 75, 184
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 4 cut(s) 39, 60, 631, 712
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 2 cut(s) 244, 443
BslFI GGGAC 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 2 cut(s) 90, 844
BsmBI CGTCTC 1 cut(s) 844
BsmFI GGGAC 1 cut(s) 120
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 5 cut(s) 39, 60, 536, 631, 712
Bsp143I GATC 6 cut(s) 48, 223, 349, 596, 694, 769
Bsp1720I GCTNAGC 1 cut(s) 618
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 2 cut(s) 584, 610
BspDI ATCGAT 1 cut(s) 51
BspFNI CGCG 2 cut(s) 75, 184
BspLI GGNNCC 1 cut(s) 304
BspMAI CTGCAG 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 1 cut(s) 231
BspTNI GGTCTC 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 243
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 3 cut(s) 101, 306, 461
BssMI GATC 6 cut(s) 48, 223, 349, 596, 694, 769
BssT1I CCWWGG 2 cut(s) 101, 306
Bst6I CTCTTC 1 cut(s) 685
BstAPI GCANNNNNTGC 1 cut(s) 716
BstBAI YACGTR 2 cut(s) 632, 713
BstC8I GCNNGC 5 cut(s) 7, 178, 182, 245, 708
BstDEI CTNAG 3 cut(s) 571, 618, 785
BstF5I GGATG 3 cut(s) 280, 316, 409
BstFNI CGCG 2 cut(s) 75, 184
BstH2I RGCGCY 2 cut(s) 12, 177
BstHHI GCGC 3 cut(s) 11, 75, 176
BstKTI GATC 6 cut(s) 51, 226, 352, 599, 697, 772
BstMAI GTCTC 2 cut(s) 90, 844
BstMBI GATC 6 cut(s) 48, 223, 349, 596, 694, 769
BstMCI CGRYCG 1 cut(s) 141
BstNSI RCATGY 2 cut(s) 458, 527
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 536
BstUI CGCG 2 cut(s) 75, 184
BstV1I GCAGC 3 cut(s) 130, 431, 706
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BstZI CGGCCG 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 51
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 1 cut(s) 140
BsuTUI ATCGAT 1 cut(s) 51
BtsCI GGATG 3 cut(s) 280, 316, 409
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 361, 767
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 5 cut(s) 7, 178, 182, 245, 708
CfoI GCGC 3 cut(s) 11, 75, 176
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 817
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 4 cut(s) 413, 455, 524, 589
DdeI CTNAG 3 cut(s) 571, 618, 785
DpnI GATC 6 cut(s) 50, 225, 351, 598, 696, 771
DpnII GATC 6 cut(s) 48, 223, 349, 596, 694, 769
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 685
Ecl136II GAGCTC 1 cut(s) 58
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 2 cut(s) 101, 306
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 817
Eco52I CGGCCG 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 318, 743, 750
Eco72I CACGTG 2 cut(s) 632, 713
Eco88I CYCGRG 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 58
EcoT14I CCWWGG 2 cut(s) 101, 306
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 101, 306
Esp3I CGTCTC 1 cut(s) 844
FaeI CATG 4 cut(s) 416, 458, 527, 592
FaiI YATR 9 cut(s) 414, 456, 525, 563, 590, 672, 777, 797, 834
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 120
FatI CATG 4 cut(s) 412, 454, 523, 588
FauI CCCGC 2 cut(s) 161, 189
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 7 cut(s) 12, 141, 144, 147, 165, 420, 720
FokI GGATG 3 cut(s) 287, 323, 416
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 7 cut(s) 12, 141, 144, 147, 165, 420, 720
FspBI CTAG 1 cut(s) 102
GlaI GCGC 3 cut(s) 10, 74, 175
GluI GCNGC 7 cut(s) 12, 141, 144, 147, 165, 420, 720
GsaI CCCAGC 1 cut(s) 492
HaeII RGCGCY 2 cut(s) 12, 177
HaeIII GGCC 1 cut(s) 140
HapII CCGG 2 cut(s) 244, 443
HhaI GCGC 3 cut(s) 11, 75, 176
Hin1II CATG 4 cut(s) 416, 458, 527, 592
Hin6I GCGC 3 cut(s) 9, 73, 174
HinP1I GCGC 3 cut(s) 9, 73, 174
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 2 cut(s) 326, 700
HpaI GTTAAC 1 cut(s) 499
HpaII CCGG 2 cut(s) 244, 443
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 3 cut(s) 462, 654, 699
Hpy188III TCNNGA 2 cut(s) 550, 573
Hpy8I GTNNAC 1 cut(s) 499
HpyAV CCTTC 3 cut(s) 293, 316, 421
HpyCH4IV ACGT 2 cut(s) 631, 712
HpyCH4V TGCA 4 cut(s) 203, 379, 419, 565
HpyF3I CTNAG 3 cut(s) 571, 618, 785
HpySE526I ACGT 2 cut(s) 631, 712
Hsp92II CATG 4 cut(s) 416, 458, 527, 592
HspAI GCGC 3 cut(s) 9, 73, 174
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 769
KspAI GTTAAC 1 cut(s) 499
Kzo9I GATC 6 cut(s) 48, 223, 349, 596, 694, 769
LmnI GCTCC 5 cut(s) 42, 63, 154, 302, 451
LpnPI CCDG 9 cut(s) 213, 246, 257, 376, 456, 487, 502, 535, 558
Lsp1109I GCAGC 3 cut(s) 130, 431, 706
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 102
MaeII ACGT 2 cut(s) 631, 712
MalI GATC 6 cut(s) 50, 225, 351, 598, 696, 771
MbiI CCGCTC 1 cut(s) 149
MboI GATC 6 cut(s) 48, 223, 349, 596, 694, 769
MboII GAAGA 8 cut(s) 328, 331, 494, 569, 647, 702, 743, 749
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 5 cut(s) 39, 60, 536, 631, 712
MluCI AATT 4 cut(s) 373, 545, 740, 790
MlyI GAGTC 1 cut(s) 694
MmeI TCCRAC 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 2 cut(s) 395, 498
MspA1I CMGCKG 2 cut(s) 143, 257
MspI CCGG 2 cut(s) 244, 443
Mva1269I GAATGC 1 cut(s) 519
MvnI CGCG 2 cut(s) 75, 184
NaeI GCCGGC 1 cut(s) 245
NdeII GATC 6 cut(s) 48, 223, 349, 596, 694, 769
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 4 cut(s) 416, 458, 527, 592
NlaIV GGNNCC 1 cut(s) 304
NmeAIII GCCGAG 1 cut(s) 116
NspI RCATGY 2 cut(s) 458, 527
PctI GAATGC 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 7 cut(s) 13, 142, 145, 148, 166, 421, 721
PleI GAGTC 1 cut(s) 694
PmaCI CACGTG 2 cut(s) 632, 713
PmlI CACGTG 2 cut(s) 632, 713
PpsI GAGTC 1 cut(s) 694
Ppu21I YACGTR 2 cut(s) 632, 713
Psp124BI GAGCTC 1 cut(s) 60
PspCI CACGTG 2 cut(s) 632, 713
PspFI CCCAGC 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 817
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 395, 498
SatI GCNGC 7 cut(s) 12, 141, 144, 147, 165, 420, 720
Sau3AI GATC 6 cut(s) 48, 223, 349, 596, 694, 769
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 1 cut(s) 694
SduI GDGCHC 5 cut(s) 39, 60, 536, 631, 712
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 817
Sse9I AATT 4 cut(s) 373, 545, 740, 790
SspMI CTAG 1 cut(s) 102
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 2 cut(s) 101, 306
TaiI ACGT 2 cut(s) 634, 715
TaqI TCGA 2 cut(s) 51, 196
TasI AATT 4 cut(s) 373, 545, 740, 790
TauI GCSGC 4 cut(s) 14, 143, 149, 167
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 395, 498
Tru9I TTAA 2 cut(s) 395, 498
TscAI CASTG 2 cut(s) 361, 767
TseI GCWGC 3 cut(s) 143, 419, 719
TspDTI ATGAA 2 cut(s) 577, 784
TspGWI ACGGA 2 cut(s) 98, 464
TspRI CASTG 2 cut(s) 361, 767
VpaK11BI GGWCC 1 cut(s) 817
XapI RAATTY 2 cut(s) 373, 545
XceI RCATGY 2 cut(s) 458, 527
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.