Rh7BG349700

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0007408
Physical Location & Seq
Reverse (-)
36460753 .. 36464352
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG349700.1

Sequence Viewer

Length: 1269 bp
ATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAGCAGCGCGAGATTACGGAACGCCTTCGAGACCCTAGGGGCCTCCGCAAGGGAGGAGGAGGAGGCTCGGCGGCTGCCCCGCCCCGCAACTTTGGTGCCAATGGTGCTCGCCCCCGAGGCTTTACTCGACCTGCAGATAGGGCTGATGTAGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTCGAGGATGGAGAAATTGTTGAGAGTGCTGAAGGAACCAAGGATGCGAAGAAGAAGGAGTCAAGCGAGGCGGCGATAGCTGATCACAGTGATAGGAGGTTGTCAAATTCCCAACAAGGTAGCTGGTTAAGGAGGGATGGCAGCCATGGAGCAGGCAAGAAGGAATTTGACATTCCAGCAACGGGTCATGTTCCAAGGGTATTGCCAAAGCAAGAAGACCCTAGCTTGGTTAATAGGAACAAAAGAATGCTCGGGCAACTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAAACGTGCAACTCTCGGGAACAGAGGCTTTCATGCGGAGATCAAATTCTTTACAGAGAGCTGAAGAAAGAGCACGTGAAGAAAGTGAAAGACTTAGGATACAAGAGCGTGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGACTCTGCGGGCACGTGTTTCTGCGAAGACGGAAGAAAAAAAATTGGAACTGCTTTTTCTTCAGTGGAGTGAGCATCAGAAGAAACTTAGCAATTTTATAAGGACTAAGGCAGAGCCTCCAATATATTATCTACCCAAGAAACCATTGGATGAGGATGCTACCTTGACTGAGCAGCGAAAAGAAAAGGAATTCCTAGAATGGAAAGCAGCACGACGGGAGGAACTGAGCGATTATCAGAAGCAGATTGGGGAGCAGTATCTAGCAAATGTTGAAAAGGAGTTGGAGAGGTGGCAAAATGCAAGGAAAGCAAGGAAAGCAAACAATGATACGATGAACTTACAGGAAACAATGGACAAAGAGTTGGACACACATAGGCTTGAGCATGGTCCCAAGAAAAGAAAGATCCCTGATGGAAGCAACAATGAGGATGAGGATGATGCGGAAGATATCAATGTAGTGGAGGATGACATGATGGATGATGTGTTGGATGTTGATGATAACAGTAGAATGGTTGATGAAACAGCTAAATCAGATGCAGGCAATGCCAGTCCAAATGCGGAGAACATTGATTAG

Protein Analysis

422

Amino Acids

48.24

Weight (kDa)

5.96

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 165 - 292 5.3e-28 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
Acc36I ACCTGC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 161
AccII CGCG 1 cut(s) 75
AclWI GGATC 2 cut(s) 228, 1093
AcsI RAATTY 5 cut(s) 370, 428, 542, 598, 884
AcuI CTGAAG 3 cut(s) 315, 636, 740
AcvI CACGTG 2 cut(s) 629, 710
AfiI CCNNNNNNNGG 3 cut(s) 115, 446, 490
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 1 cut(s) 968
AluBI AGCT 7 cut(s) 58, 254, 344, 387, 489, 614, 1220
AluI AGCT 7 cut(s) 58, 254, 344, 387, 489, 614, 1220
Alw21I GWGCWC 4 cut(s) 39, 60, 175, 628
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 228, 1093
Ama87I CYCGRG 3 cut(s) 180, 515, 568
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 5 cut(s) 70, 140, 405, 868, 902
ApoI RAATTY 5 cut(s) 370, 428, 542, 598, 884
Asp700I GAANNNNTTC 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 2 cut(s) 11, 75
AspS9I GGNCC 2 cut(s) 106, 1082
AvaI CYCGRG 3 cut(s) 180, 515, 568
AvaII GGWCC 1 cut(s) 1082
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 2 cut(s) 533, 709
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 60
BbrPI CACGTG 2 cut(s) 629, 710
BbsI GAAGAC 2 cut(s) 486, 728
Bbv12I GWGCWC 4 cut(s) 39, 60, 175, 628
BbvI GCAGC 5 cut(s) 82, 127, 417, 880, 914
BccI CCATC 4 cut(s) 266, 395, 1100, 1162
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciVI GTATCC 1 cut(s) 645
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 4 cut(s) 102, 486, 890, 956
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 205
BfuI GTATCC 1 cut(s) 645
BglI GCCNNNNNGGC 1 cut(s) 183
BglII AGATCT 1 cut(s) 691
BisI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 8 cut(s) 13, 72, 139, 142, 337, 407, 870, 904
Bme18I GGWCC 1 cut(s) 1082
BmeT110I CYCGRG 3 cut(s) 180, 515, 568
BmgT120I GGNCC 2 cut(s) 106, 1082
BmiI GGNNCC 4 cut(s) 107, 163, 301, 1084
BmsI GCATC 5 cut(s) 298, 778, 841, 1123, 1219
BpiI GAAGAC 2 cut(s) 486, 728
BpuEI CTTGAG 1 cut(s) 1094
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 2 cut(s) 629, 710
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 101, 181, 303, 409, 458
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 446, 490
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 1242
Bse3DI GCAATG 1 cut(s) 1243
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 5 cut(s) 101, 181, 303, 409, 458
BseLI CCNNNNNNNGG 3 cut(s) 115, 446, 490
BseMI GCAATG 1 cut(s) 1243
BseMII CTCAG 2 cut(s) 855, 911
BseNI ACTGG 1 cut(s) 1242
BseRI GAGGAG 5 cut(s) 44, 135, 138, 141, 700
BseSI GKGCMC 2 cut(s) 533, 709
BseXI GCAGC 5 cut(s) 82, 127, 417, 880, 914
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 108
BshNI GGYRCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 4 cut(s) 39, 60, 175, 628
BsiHKCI CYCGRG 3 cut(s) 180, 515, 568
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 1068
BslI CCNNNNNNNGG 3 cut(s) 115, 446, 490
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 1068
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 3 cut(s) 180, 515, 568
Bsp1286I GDGCHC 6 cut(s) 39, 60, 175, 533, 628, 709
Bsp143I GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
Bsp19I CCATGG 1 cut(s) 409
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 856, 912
BspDI ATCGAT 1 cut(s) 51
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 4 cut(s) 107, 163, 301, 1084
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 2 cut(s) 228, 1093
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 1243
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 1242
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 5 cut(s) 101, 181, 303, 409, 458
BssMI GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
BssT1I CCWWGG 4 cut(s) 101, 303, 409, 458
Bst4CI ACNGT 2 cut(s) 353, 1199
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstBAI YACGTR 2 cut(s) 629, 710
BstC8I GCNNGC 7 cut(s) 7, 68, 175, 242, 418, 705, 1234
BstDEI CTNAG 5 cut(s) 647, 782, 801, 864, 920
BstDSI CCRYGG 1 cut(s) 409
BstENI CCTNNNNNAGG 1 cut(s) 113
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 75
BstKTI GATC 6 cut(s) 51, 223, 349, 596, 694, 1101
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
BstMWI GCNNNNNNNGC 9 cut(s) 11, 72, 170, 183, 206, 341, 1001, 1010, 1238
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 2 cut(s) 533, 709
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 5 cut(s) 82, 127, 417, 880, 914
BstV2I GAAGAC 2 cut(s) 486, 728
BstX2I RGATCY 2 cut(s) 691, 1098
BstYI RGATCY 2 cut(s) 691, 1098
Bsu15I ATCGAT 1 cut(s) 51
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 358, 764
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 7 cut(s) 7, 68, 175, 242, 418, 705, 1234
CfoI GCGC 2 cut(s) 11, 75
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 106, 1082
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 5 cut(s) 410, 452, 586, 1079, 1165
DdeI CTNAG 5 cut(s) 647, 782, 801, 864, 920
DpnI GATC 6 cut(s) 50, 222, 348, 595, 693, 1100
DpnII GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 4 cut(s) 101, 303, 409, 458
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 90
Eco32I GATATC 1 cut(s) 1144
Eco47I GGWCC 1 cut(s) 1082
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 315, 636, 740
Eco72I CACGTG 2 cut(s) 629, 710
Eco88I CYCGRG 3 cut(s) 180, 515, 568
EcoICRI GAGCTC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 106
EcoRI GAATTC 1 cut(s) 884
EcoRV GATATC 1 cut(s) 1144
EcoT14I CCWWGG 4 cut(s) 101, 303, 409, 458
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 4 cut(s) 101, 303, 409, 458
FaeI CATG 5 cut(s) 413, 455, 589, 1082, 1168
FaiI YATR 8 cut(s) 411, 453, 587, 794, 820, 1068, 1080, 1166
FaqI GGGAC 1 cut(s) 1068
FatI CATG 5 cut(s) 409, 451, 585, 1078, 1164
FauI CCCGC 3 cut(s) 153, 158, 696
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
FspBI CTAG 4 cut(s) 102, 486, 890, 956
GlaI GCGC 2 cut(s) 10, 74
GluI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 241
HhaI GCGC 2 cut(s) 11, 75
Hin1II CATG 5 cut(s) 413, 455, 589, 1082, 1168
Hin6I GCGC 2 cut(s) 9, 73
HinP1I GCGC 2 cut(s) 9, 73
HinfI GANTC 2 cut(s) 323, 697
HpaII CCGG 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 4 cut(s) 696, 774, 933, 1228
Hpy188III TCNNGA 3 cut(s) 95, 547, 570
Hpy8I GTNNAC 1 cut(s) 665
Hpy99I CGWCG 1 cut(s) 912
HpyAV CCTTC 4 cut(s) 101, 290, 313, 418
HpyCH4III ACNGT 2 cut(s) 353, 1199
HpyCH4IV ACGT 3 cut(s) 558, 628, 709
HpyCH4V TGCA 4 cut(s) 200, 562, 995, 1232
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 72, 170, 183, 206, 341, 1001, 1010, 1238
HpyF3I CTNAG 5 cut(s) 647, 782, 801, 864, 920
HpySE526I ACGT 3 cut(s) 558, 628, 709
Hsp92II CATG 5 cut(s) 413, 455, 589, 1082, 1168
HspAI GCGC 2 cut(s) 9, 73
KroI GCCGGC 1 cut(s) 240
KroNI GCCGGC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
LmnI GCTCC 4 cut(s) 42, 63, 413, 946
Lsp1109I GCAGC 5 cut(s) 82, 127, 417, 880, 914
LweI GCATC 5 cut(s) 298, 778, 841, 1123, 1219
MaeI CTAG 4 cut(s) 102, 486, 890, 956
MaeII ACGT 3 cut(s) 558, 628, 709
MalI GATC 6 cut(s) 50, 222, 348, 595, 693, 1100
MboI GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
MflI RGATCY 2 cut(s) 691, 1098
MhlI GDGCHC 6 cut(s) 39, 60, 175, 533, 628, 709
MluCI AATT 9 cut(s) 279, 370, 428, 542, 598, 669, 737, 787, 884
MlyI GAGTC 2 cut(s) 332, 691
MmeI TCCRAC 4 cut(s) 85, 957, 1038, 1161
MroNI GCCGGC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 2 cut(s) 392, 495
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 516
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 9 cut(s) 11, 72, 170, 183, 206, 341, 1001, 1010, 1238
NaeI GCCGGC 1 cut(s) 242
NcoI CCATGG 1 cut(s) 409
NdeII GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
NgoMIV GCCGGC 1 cut(s) 240
NlaIII CATG 5 cut(s) 413, 455, 589, 1082, 1168
NlaIV GGNNCC 4 cut(s) 107, 163, 301, 1084
NmeAIII GCCGAG 1 cut(s) 113
PctI GAATGC 1 cut(s) 516
PdiI GCCGGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 90
PkrI GCNGC 8 cut(s) 13, 72, 139, 142, 337, 407, 870, 904
PleI GAGTC 2 cut(s) 331, 691
PmaCI CACGTG 2 cut(s) 629, 710
PmlI CACGTG 2 cut(s) 629, 710
PpsI GAGTC 2 cut(s) 331, 691
Ppu21I YACGTR 2 cut(s) 629, 710
PsiI TTATAA 1 cut(s) 794
Psp124BI GAGCTC 1 cut(s) 60
PspCI CACGTG 2 cut(s) 629, 710
PspN4I GGNNCC 4 cut(s) 107, 163, 301, 1084
PspPI GGNCC 2 cut(s) 106, 1082
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 691, 1098
PvuII CAGCTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 392, 495
SatI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
Sau3AI GATC 6 cut(s) 48, 220, 346, 593, 691, 1098
Sau96I GGNCC 2 cut(s) 106, 1082
SchI GAGTC 2 cut(s) 332, 691
SduI GDGCHC 6 cut(s) 39, 60, 175, 533, 628, 709
SfaNI GCATC 5 cut(s) 298, 778, 841, 1123, 1219
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
Sse9I AATT 9 cut(s) 279, 370, 428, 542, 598, 669, 737, 787, 884
SspMI CTAG 4 cut(s) 102, 486, 890, 956
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 4 cut(s) 101, 303, 409, 458
TaaI ACNGT 2 cut(s) 353, 1199
TaiI ACGT 3 cut(s) 561, 631, 712
TaqI TCGA 4 cut(s) 51, 94, 193, 267
TasI AATT 9 cut(s) 279, 370, 428, 542, 598, 669, 737, 787, 884
TauI GCSGC 3 cut(s) 14, 140, 338
Tru1I TTAA 2 cut(s) 392, 495
Tru9I TTAA 2 cut(s) 392, 495
TscAI CASTG 2 cut(s) 358, 764
TseI GCWGC 5 cut(s) 70, 140, 405, 868, 902
TspDTI ATGAA 3 cut(s) 574, 1043, 1227
TspGWI ACGGA 2 cut(s) 98, 740
TspRI CASTG 2 cut(s) 358, 764
VpaK11BI GGWCC 1 cut(s) 1082
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 5 cut(s) 370, 428, 542, 598, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 4 cut(s) 102, 486, 890, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.