RLG00000001987

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007408
Physical Location & Seq
Forward (+)
23980572 .. 23984369
3798 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001987

Sequence Viewer

Length: 1275 bp
ATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAGCAGCGAGAGATTACGGAACGCCTTCGAGACCCTAGGGGACTCCGCAAGGGAGGAGGAGGAGGCTCGGCGGCTGCCCCGCCCCGCAACTTTGGTGCCAATGGTGCTCGCCCCCGAGGCTTTACTCGACCTGCAGATAGGGCTGATGTGGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTCGAGGATGGAGAAATTGTTGAGAGTGCTGAAGGAGCCAAGGATGCAAAGAAGAAGGATTCAAGCGAGGCGGCGATAGCTGATCACAGTGATAGTGATAGGAGGTTGTCAAATTCCCAACAAGGTAGCTGGTTAAGGAGGGATGGCAGCCATGGAGCAGGAAAGAAGGAATATGACATTCCAGCAACGGATCATGTTCCAAGGGTATTGCCAAAGCAAGAAGACCCTAGCTTGGTTAATAGGAACAAAAGAATGCTCGGGCAACTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAAACGTGCAACTCTCAGGAACAGAGGCTTTCATGCGGAGATCAAATTCTTTACAGAGAGCTGAAGAAAGAGCACGTGAAGAAAGTGAAAGACTTAGGATACAAGAGCGTGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGACTCTGCGGGCACGTGTTTCTGCCAAGACGGAAGAAAAAAAATTGGAACTGCTTTTTCTTCAGTGGAGTGAGCATCACAAGAAACTTAGCAATTTTATAAGGACCAAGGCAGAGCCTCCAATATATTATCTACCCAAGAAACCATTGGATGAGGATGCTACCTTGGCTGAGCAGCGAAAAGAAAAGGAATTCCTAGAATGGAAAGCAGCACGACGGGAGGAACTGAGCGATTATCAGAAGCAGATTGGGGAGCAGTATCTAGCAAATGTTGAAAAGGAGTTGGAGAGGTGGCAAAATGCAAGGAAAGCAAGGAAAGCAAACAATGATACGATGAACTTACAGGAAACAATGGACAAAGAGTTGGACACACATAGGCTTGAGCATGGTCCCAAGAAAAGAAAGATCCCTGATGGAAGCAACAATGAGGATGAGGATGATGTGGAAGATATCAATGTAGTGGAGGATGACATGATGGATGATGTATTGGATGTTGATGATAACAGTAGAATGGTTGATGAAACAGCTAAATCAGATGCAGGTAATGCCAGTCCAAATGCGGATAACGTTGATTAG

Protein Analysis

425

Amino Acids

48.45

Weight (kDa)

5.8

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 167 - 294 5.4e-28 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 800
Acc36I ACCTGC 2 cut(s) 205, 1229
AccB1I GGYRCC 1 cut(s) 161
AclI AACGTT 1 cut(s) 1266
AclWI GGATC 3 cut(s) 228, 462, 1099
AcsI RAATTY 4 cut(s) 376, 548, 604, 890
AcuI CTGAAG 3 cut(s) 315, 642, 746
AcvI CACGTG 2 cut(s) 635, 716
AfiI CCNNNNNNNGG 1 cut(s) 496
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 2 cut(s) 327, 974
AluBI AGCT 7 cut(s) 58, 254, 344, 393, 495, 620, 1226
AluI AGCT 7 cut(s) 58, 254, 344, 393, 495, 620, 1226
Alw21I GWGCWC 4 cut(s) 39, 60, 175, 634
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 3 cut(s) 228, 462, 1099
Ama87I CYCGRG 2 cut(s) 180, 521
ApeKI GCWGC 5 cut(s) 70, 140, 411, 874, 908
ApoI RAATTY 4 cut(s) 376, 548, 604, 890
Asp700I GAANNNNTTC 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 804, 1088
AvaI CYCGRG 2 cut(s) 180, 521
AvaII GGWCC 2 cut(s) 804, 1088
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 2 cut(s) 539, 715
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 60
BbrPI CACGTG 2 cut(s) 635, 716
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 4 cut(s) 39, 60, 175, 634
BbvI GCAGC 5 cut(s) 82, 127, 423, 886, 920
BccI CCATC 4 cut(s) 266, 401, 1106, 1168
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciVI GTATCC 1 cut(s) 651
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 4 cut(s) 102, 492, 896, 962
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 2 cut(s) 205, 1229
BfuI GTATCC 1 cut(s) 651
BglI GCCNNNNNGGC 1 cut(s) 183
BglII AGATCT 1 cut(s) 697
BisI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 412, 875, 909
BlnI CCTAGG 1 cut(s) 101
BlpI GCTNAGC 1 cut(s) 870
BlsI GCNGC 8 cut(s) 13, 72, 139, 142, 337, 413, 876, 910
Bme18I GGWCC 2 cut(s) 804, 1088
BmeT110I CYCGRG 2 cut(s) 180, 521
BmgT120I GGNCC 2 cut(s) 804, 1088
BmiI GGNNCC 3 cut(s) 163, 301, 1090
BmsI GCATC 4 cut(s) 298, 784, 847, 1225
BpiI GAAGAC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 1100
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 2 cut(s) 635, 716
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 7 cut(s) 101, 181, 303, 415, 464, 807, 864
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 1 cut(s) 496
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 1248
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 7 cut(s) 101, 181, 303, 415, 464, 807, 864
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 588, 861, 917
BseNI ACTGG 1 cut(s) 1248
BseRI GAGGAG 5 cut(s) 44, 135, 138, 141, 706
BseSI GKGCMC 2 cut(s) 539, 715
BseXI GCAGC 5 cut(s) 82, 127, 423, 886, 920
BshNI GGYRCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 4 cut(s) 39, 60, 175, 634
BsiHKCI CYCGRG 2 cut(s) 180, 521
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 2 cut(s) 120, 1074
BslI CCNNNNNNNGG 1 cut(s) 496
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 2 cut(s) 120, 1074
BsmI GAATGC 1 cut(s) 522
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 2 cut(s) 180, 521
Bsp1286I GDGCHC 6 cut(s) 39, 60, 175, 539, 634, 715
Bsp143I GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
Bsp1720I GCTNAGC 1 cut(s) 870
Bsp19I CCATGG 1 cut(s) 415
BspCNI CTCAG 3 cut(s) 587, 862, 918
BspDI ATCGAT 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 163, 301, 1090
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 2 cut(s) 205, 1229
BspPI GGATC 3 cut(s) 228, 462, 1099
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 1248
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 7 cut(s) 101, 181, 303, 415, 464, 807, 864
BssMI GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
BssT1I CCWWGG 6 cut(s) 101, 303, 415, 464, 807, 864
Bst4CI ACNGT 2 cut(s) 353, 1205
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstBAI YACGTR 2 cut(s) 635, 716
BstC8I GCNNGC 5 cut(s) 7, 68, 175, 242, 711
BstDEI CTNAG 5 cut(s) 574, 653, 788, 870, 926
BstDSI CCRYGG 1 cut(s) 415
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 7 cut(s) 51, 223, 349, 457, 602, 700, 1107
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 2 cut(s) 539, 715
BstV1I GCAGC 5 cut(s) 82, 127, 423, 886, 920
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 2 cut(s) 697, 1104
BstYI RGATCY 2 cut(s) 697, 1104
Bsu15I ATCGAT 1 cut(s) 51
BsuI GTATCC 1 cut(s) 651
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 415
BtsIMutI CAGTG 2 cut(s) 358, 770
BveI ACCTGC 2 cut(s) 205, 1229
Cac8I GCNNGC 5 cut(s) 7, 68, 175, 242, 711
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 804, 1088
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 5 cut(s) 416, 458, 592, 1085, 1171
DdeI CTNAG 5 cut(s) 574, 653, 788, 870, 926
DpnI GATC 7 cut(s) 50, 222, 348, 456, 601, 699, 1106
DpnII GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 6 cut(s) 101, 303, 415, 464, 807, 864
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 90
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 2 cut(s) 804, 1088
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 315, 642, 746
Eco72I CACGTG 2 cut(s) 635, 716
Eco88I CYCGRG 2 cut(s) 180, 521
EcoICRI GAGCTC 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 890
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 6 cut(s) 101, 303, 415, 464, 807, 864
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 6 cut(s) 101, 303, 415, 464, 807, 864
FaeI CATG 5 cut(s) 419, 461, 595, 1088, 1174
FaiI YATR 9 cut(s) 417, 438, 459, 593, 800, 826, 1074, 1086, 1172
FaqI GGGAC 2 cut(s) 120, 1074
FatI CATG 5 cut(s) 415, 457, 591, 1084, 1170
FauI CCCGC 3 cut(s) 153, 158, 702
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 412, 875, 909
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 412, 875, 909
FspBI CTAG 4 cut(s) 102, 492, 896, 962
GlaI GCGC 1 cut(s) 10
GluI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 412, 875, 909
HaeII RGCGCY 1 cut(s) 12
HapII CCGG 1 cut(s) 241
HhaI GCGC 1 cut(s) 11
Hin1II CATG 5 cut(s) 419, 461, 595, 1088, 1174
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 3 cut(s) 108, 323, 703
HpaII CCGG 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 671
Hpy188I TCNGA 3 cut(s) 702, 939, 1234
Hpy188III TCNNGA 3 cut(s) 95, 553, 576
Hpy8I GTNNAC 1 cut(s) 671
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 4 cut(s) 101, 290, 313, 424
HpyCH4III ACNGT 2 cut(s) 353, 1205
HpyCH4IV ACGT 4 cut(s) 564, 634, 715, 1266
HpyCH4V TGCA 5 cut(s) 200, 311, 568, 1001, 1238
HpyF3I CTNAG 5 cut(s) 574, 653, 788, 870, 926
HpySE526I ACGT 4 cut(s) 564, 634, 715, 1266
Hsp92II CATG 5 cut(s) 419, 461, 595, 1088, 1174
HspAI GCGC 1 cut(s) 9
KroI GCCGGC 1 cut(s) 240
KroNI GCCGGC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
LmnI GCTCC 5 cut(s) 42, 63, 299, 419, 952
Lsp1109I GCAGC 5 cut(s) 82, 127, 423, 886, 920
LweI GCATC 4 cut(s) 298, 784, 847, 1225
MaeI CTAG 4 cut(s) 102, 492, 896, 962
MaeII ACGT 4 cut(s) 564, 634, 715, 1266
MalI GATC 7 cut(s) 50, 222, 348, 456, 601, 699, 1106
MboI GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
MboII GAAGA 7 cut(s) 328, 497, 635, 650, 746, 752, 1157
MflI RGATCY 2 cut(s) 697, 1104
MhlI GDGCHC 6 cut(s) 39, 60, 175, 539, 634, 715
MluCI AATT 8 cut(s) 279, 376, 548, 604, 675, 743, 793, 890
MlyI GAGTC 2 cut(s) 102, 697
MmeI TCCRAC 3 cut(s) 85, 963, 1044
MroNI GCCGGC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 2 cut(s) 398, 501
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 522
NaeI GCCGGC 1 cut(s) 242
NcoI CCATGG 1 cut(s) 415
NdeII GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
NgoMIV GCCGGC 1 cut(s) 240
NlaIII CATG 5 cut(s) 419, 461, 595, 1088, 1174
NlaIV GGNNCC 3 cut(s) 163, 301, 1090
NmeAIII GCCGAG 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 70
PctI GAATGC 1 cut(s) 522
PdiI GCCGGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 90
PfeI GAWTC 1 cut(s) 323
PkrI GCNGC 8 cut(s) 13, 72, 139, 142, 337, 413, 876, 910
PleI GAGTC 2 cut(s) 102, 697
PmaCI CACGTG 2 cut(s) 635, 716
PmlI CACGTG 2 cut(s) 635, 716
PpsI GAGTC 2 cut(s) 102, 697
Ppu21I YACGTR 2 cut(s) 635, 716
PsiI TTATAA 1 cut(s) 800
Psp124BI GAGCTC 1 cut(s) 60
Psp1406I AACGTT 1 cut(s) 1266
PspCI CACGTG 2 cut(s) 635, 716
PspN4I GGNNCC 3 cut(s) 163, 301, 1090
PspPI GGNCC 2 cut(s) 804, 1088
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 697, 1104
PvuII CAGCTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 398, 501
SatI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 412, 875, 909
Sau3AI GATC 7 cut(s) 48, 220, 346, 454, 599, 697, 1104
Sau96I GGNCC 2 cut(s) 804, 1088
SchI GAGTC 2 cut(s) 102, 697
SduI GDGCHC 6 cut(s) 39, 60, 175, 539, 634, 715
SfaNI GCATC 4 cut(s) 298, 784, 847, 1225
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 2 cut(s) 804, 1088
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
Sse9I AATT 8 cut(s) 279, 376, 548, 604, 675, 743, 793, 890
SspMI CTAG 4 cut(s) 102, 492, 896, 962
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 6 cut(s) 101, 303, 415, 464, 807, 864
TaaI ACNGT 2 cut(s) 353, 1205
TaiI ACGT 4 cut(s) 567, 637, 718, 1269
TaqI TCGA 4 cut(s) 51, 94, 193, 267
TasI AATT 8 cut(s) 279, 376, 548, 604, 675, 743, 793, 890
TauI GCSGC 3 cut(s) 14, 140, 338
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 2 cut(s) 398, 501
Tru9I TTAA 2 cut(s) 398, 501
TscAI CASTG 2 cut(s) 358, 770
TseI GCWGC 5 cut(s) 70, 140, 411, 874, 908
TspDTI ATGAA 3 cut(s) 580, 1049, 1233
TspGWI ACGGA 3 cut(s) 98, 467, 746
TspRI CASTG 2 cut(s) 358, 770
VpaK11BI GGWCC 2 cut(s) 804, 1088
XapI RAATTY 4 cut(s) 376, 548, 604, 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 4 cut(s) 102, 492, 896, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.