RchiOBHm_Chr7g0223331

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007408
Physical Location & Seq
Reverse (-)
45002628 .. 45007748
5121 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19996

Sequence Viewer

Length: 1272 bp
ATGATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAGCAGCGCGAGATTACGGAACGCCTTCGAGACCCTAGGGGACTCCGCAAGGGAGGAGGAGGAGGCTCGGCGGCTGCCCCGCCCCGCAACTTTGGTGCCAACGGTGCTCGCCCCCGAGGCTTTACTCGACCTGCAGATAGGGCTGATGTGGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTCGAGGATGGAGAAATTGTTGAGAGTGCTGAAGGAACCAAGGATGCAAAGAATAAGGAGTCAAGCGAGGCGGCGATAGCTGATCACAGTGATAGGAGGTTGTCAAATTCCCAACAAGGTAGCTGGTTAAGGAGGGATGGCAGCCATGGAGCAGGCAAGAAGGAATTTGACATTCCAGCAACGGATCATGTTCCAAGGGTATTGCCAAAGCAAGAAGACCCTAGCTTGGTCAATAGGAACAAAAGAATGCTCGGGCAACTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAAACGTGCAACTCTCAGGAACAGAAGCTTTCATGCGGAGATCGAATTCTTTACAAAGAGCTGAAGAAAGAGCACGTGAAGAAAGTGAAAGACTTAGGATACAAGAGCGCGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGACTCTGCGGGCACGTGTTTCTGCCAAGACAGAAGAAAAAAAATTGGAACTGCTTTTTCTTCAGTGGAGTGAGCATCACAAGAAACTTAGCAATTTTATAAGGACTAAGGCAGAGCCTCCAATATATTATCTACCCAAGAAACCATTGGATGAGGATGCTACCTTGGCTGAGCAGCGAAAAGAAAAGGAATTCCTAGAATGGAAAGCAGCACGAAGGGAGGAACTGAGCAATTATCAGAAGCAGATTGGGGAGCAGTATCTAGCAAATGTTGAAAAGGAGTTGGAGAGGTGGCAAAATGCAAGGAAAGCAAGGAAAGCAAACAATGATACGATGAACTTACAGGAAACAATGGACAAAGAATTGGACACACATAGGCTTGAGCATGGTCCCAAGAAAAGAAAGATCCCTGATGGAAGCAACAATGAGGATGAGGATGATGTGGAAGATATCAATGTAGTGGAGGATGACCTGATGGATGATGTGTTGGATGTTGATGATAACAGTAGAATGGTTGATGAAACAGCTAAATCAGATGCAGGCAATGCCAGTCCAAATGCGGAGAATGTTGATTAG

Protein Analysis

423

Amino Acids

48.39

Weight (kDa)

5.9

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 166 - 293 5.3e-28 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 2 cut(s) 78, 666
AclWI GGATC 3 cut(s) 231, 459, 1096
AcsI RAATTY 5 cut(s) 373, 431, 545, 601, 887
AcuI CTGAAG 3 cut(s) 318, 639, 743
AcvI CACGTG 2 cut(s) 632, 713
AfiI CCNNNNNNNGG 1 cut(s) 493
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 1 cut(s) 971
AluBI AGCT 8 cut(s) 61, 257, 347, 390, 492, 584, 617, 1223
AluI AGCT 8 cut(s) 61, 257, 347, 390, 492, 584, 617, 1223
Alw21I GWGCWC 4 cut(s) 42, 63, 178, 631
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 3 cut(s) 231, 459, 1096
Ama87I CYCGRG 2 cut(s) 183, 518
ApeKI GCWGC 5 cut(s) 73, 143, 408, 871, 905
ApoI RAATTY 5 cut(s) 373, 431, 545, 601, 887
Asp700I GAANNNNTTC 1 cut(s) 93
AspA2I CCTAGG 1 cut(s) 104
AspLEI GCGC 3 cut(s) 14, 78, 666
AspS9I GGNCC 1 cut(s) 1085
AvaI CYCGRG 2 cut(s) 183, 518
AvaII GGWCC 1 cut(s) 1085
AvrII CCTAGG 1 cut(s) 104
BaeGI GKGCMC 2 cut(s) 536, 712
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 63
BbrPI CACGTG 2 cut(s) 632, 713
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 4 cut(s) 42, 63, 178, 631
BbvI GCAGC 5 cut(s) 85, 130, 420, 883, 917
BccI CCATC 4 cut(s) 269, 398, 1103, 1165
BcgI CGANNNNNNTGC 4 cut(s) 34, 68, 656, 690
BciVI GTATCC 1 cut(s) 648
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 4 cut(s) 105, 489, 893, 959
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 208
BfuI GTATCC 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 186
BglII AGATCT 1 cut(s) 694
BisI GCNGC 8 cut(s) 15, 74, 141, 144, 339, 409, 872, 906
BlnI CCTAGG 1 cut(s) 104
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 8 cut(s) 16, 75, 142, 145, 340, 410, 873, 907
Bme18I GGWCC 1 cut(s) 1085
BmeT110I CYCGRG 2 cut(s) 183, 518
BmgT120I GGNCC 1 cut(s) 1085
BmiI GGNNCC 3 cut(s) 166, 304, 1087
BmsI GCATC 4 cut(s) 301, 781, 844, 1222
BpiI GAAGAC 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 1097
Bsa29I ATCGAT 1 cut(s) 54
BsaAI YACGTR 2 cut(s) 632, 713
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 6 cut(s) 104, 184, 306, 412, 461, 861
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 1245
Bse3DI GCAATG 1 cut(s) 1246
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 6 cut(s) 104, 184, 306, 412, 461, 861
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 1 cut(s) 1246
BseMII CTCAG 3 cut(s) 585, 858, 914
BseNI ACTGG 1 cut(s) 1245
BseRI GAGGAG 5 cut(s) 47, 138, 141, 144, 703
BseSI GKGCMC 2 cut(s) 536, 712
BseXI GCAGC 5 cut(s) 85, 130, 420, 883, 917
Bsh1236I CGCG 2 cut(s) 78, 666
BshNI GGYRCC 1 cut(s) 164
BshVI ATCGAT 1 cut(s) 54
BsiHKAI GWGCWC 4 cut(s) 42, 63, 178, 631
BsiHKCI CYCGRG 2 cut(s) 183, 518
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 2 cut(s) 123, 1071
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 123, 1071
BsmI GAATGC 1 cut(s) 519
Bso31I GGTCTC 1 cut(s) 93
BsoBI CYCGRG 2 cut(s) 183, 518
Bsp1286I GDGCHC 6 cut(s) 42, 63, 178, 536, 631, 712
Bsp143I GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
Bsp1720I GCTNAGC 1 cut(s) 867
Bsp19I CCATGG 1 cut(s) 412
BspCNI CTCAG 3 cut(s) 584, 859, 915
BspDI ATCGAT 1 cut(s) 54
BspFNI CGCG 2 cut(s) 78, 666
BspLI GGNNCC 3 cut(s) 166, 304, 1087
BspMAI CTGCAG 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 3 cut(s) 231, 459, 1096
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 1246
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 1245
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 6 cut(s) 104, 184, 306, 412, 461, 861
BssMI GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
BssT1I CCWWGG 5 cut(s) 104, 306, 412, 461, 861
Bst4CI ACNGT 3 cut(s) 173, 356, 1202
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstBAI YACGTR 2 cut(s) 632, 713
BstC8I GCNNGC 7 cut(s) 10, 71, 178, 245, 421, 708, 1237
BstDEI CTNAG 6 cut(s) 571, 650, 785, 804, 867, 923
BstDSI CCRYGG 1 cut(s) 412
BstFNI CGCG 2 cut(s) 78, 666
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 3 cut(s) 14, 78, 666
BstKTI GATC 7 cut(s) 54, 226, 352, 454, 599, 697, 1104
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 2 cut(s) 536, 712
BstUI CGCG 2 cut(s) 78, 666
BstV1I GCAGC 5 cut(s) 85, 130, 420, 883, 917
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 2 cut(s) 694, 1101
BstYI RGATCY 2 cut(s) 694, 1101
Bsu15I ATCGAT 1 cut(s) 54
BsuI GTATCC 1 cut(s) 648
BsuTUI ATCGAT 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 361, 767
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 7 cut(s) 10, 71, 178, 245, 421, 708, 1237
CfoI GCGC 3 cut(s) 14, 78, 666
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 1085
ClaI ATCGAT 1 cut(s) 54
CviAII CATG 4 cut(s) 413, 455, 589, 1082
DdeI CTNAG 6 cut(s) 571, 650, 785, 804, 867, 923
DpnI GATC 7 cut(s) 53, 225, 351, 453, 598, 696, 1103
DpnII GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 5 cut(s) 104, 306, 412, 461, 861
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 1147
Eco47I GGWCC 1 cut(s) 1085
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 3 cut(s) 318, 639, 743
Eco72I CACGTG 2 cut(s) 632, 713
Eco88I CYCGRG 2 cut(s) 183, 518
EcoICRI GAGCTC 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 601, 887
EcoRV GATATC 1 cut(s) 1147
EcoT14I CCWWGG 5 cut(s) 104, 306, 412, 461, 861
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 5 cut(s) 104, 306, 412, 461, 861
FaeI CATG 4 cut(s) 416, 458, 592, 1085
FaiI YATR 7 cut(s) 414, 456, 590, 797, 823, 1071, 1083
FaqI GGGAC 2 cut(s) 123, 1071
FatI CATG 4 cut(s) 412, 454, 588, 1081
FauI CCCGC 3 cut(s) 156, 161, 699
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 8 cut(s) 15, 74, 141, 144, 339, 409, 872, 906
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 8 cut(s) 15, 74, 141, 144, 339, 409, 872, 906
FspBI CTAG 4 cut(s) 105, 489, 893, 959
GlaI GCGC 3 cut(s) 13, 77, 665
GluI GCNGC 8 cut(s) 15, 74, 141, 144, 339, 409, 872, 906
HaeII RGCGCY 1 cut(s) 15
HapII CCGG 1 cut(s) 244
HhaI GCGC 3 cut(s) 14, 78, 666
Hin1II CATG 4 cut(s) 416, 458, 592, 1085
Hin6I GCGC 3 cut(s) 12, 76, 664
HinP1I GCGC 3 cut(s) 12, 76, 664
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 3 cut(s) 111, 326, 700
HpaII CCGG 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 699, 936, 1231
Hpy188III TCNNGA 3 cut(s) 98, 550, 573
HpyAV CCTTC 4 cut(s) 104, 293, 421, 906
HpyCH4III ACNGT 3 cut(s) 173, 356, 1202
HpyCH4IV ACGT 3 cut(s) 561, 631, 712
HpyCH4V TGCA 5 cut(s) 203, 314, 565, 998, 1235
HpyF3I CTNAG 6 cut(s) 571, 650, 785, 804, 867, 923
HpySE526I ACGT 3 cut(s) 561, 631, 712
Hsp92II CATG 4 cut(s) 416, 458, 592, 1085
HspAI GCGC 3 cut(s) 12, 76, 664
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
LmnI GCTCC 4 cut(s) 45, 66, 416, 949
Lsp1109I GCAGC 5 cut(s) 85, 130, 420, 883, 917
LweI GCATC 4 cut(s) 301, 781, 844, 1222
MaeI CTAG 4 cut(s) 105, 489, 893, 959
MaeII ACGT 3 cut(s) 561, 631, 712
MalI GATC 7 cut(s) 53, 225, 351, 453, 598, 696, 1103
MboI GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
MboII GAAGA 6 cut(s) 494, 632, 647, 743, 749, 1154
MflI RGATCY 2 cut(s) 694, 1101
MhlI GDGCHC 6 cut(s) 42, 63, 178, 536, 631, 712
MlyI GAGTC 3 cut(s) 105, 335, 694
MmeI TCCRAC 3 cut(s) 88, 960, 1164
MroNI GCCGGC 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 244
Mva1269I GAATGC 1 cut(s) 519
MvnI CGCG 2 cut(s) 78, 666
NaeI GCCGGC 1 cut(s) 245
NcoI CCATGG 1 cut(s) 412
NdeII GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 4 cut(s) 416, 458, 592, 1085
NlaIV GGNNCC 3 cut(s) 166, 304, 1087
NmeAIII GCCGAG 1 cut(s) 116
PctI GAATGC 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 93
PkrI GCNGC 8 cut(s) 16, 75, 142, 145, 340, 410, 873, 907
PleI GAGTC 3 cut(s) 105, 334, 694
PmaCI CACGTG 2 cut(s) 632, 713
PmlI CACGTG 2 cut(s) 632, 713
PpsI GAGTC 3 cut(s) 105, 334, 694
Ppu21I YACGTR 2 cut(s) 632, 713
PsiI TTATAA 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 63
PspCI CACGTG 2 cut(s) 632, 713
PspN4I GGNNCC 3 cut(s) 166, 304, 1087
PspPI GGNCC 1 cut(s) 1085
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 2 cut(s) 694, 1101
PvuII CAGCTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 8 cut(s) 15, 74, 141, 144, 339, 409, 872, 906
Sau3AI GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
Sau96I GGNCC 1 cut(s) 1085
SchI GAGTC 3 cut(s) 105, 335, 694
SduI GDGCHC 6 cut(s) 42, 63, 178, 536, 631, 712
SfaNI GCATC 4 cut(s) 301, 781, 844, 1222
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 1085
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
SspMI CTAG 4 cut(s) 105, 489, 893, 959
SstI GAGCTC 1 cut(s) 63
StyI CCWWGG 5 cut(s) 104, 306, 412, 461, 861
TaaI ACNGT 3 cut(s) 173, 356, 1202
TaiI ACGT 3 cut(s) 564, 634, 715
TaqI TCGA 5 cut(s) 54, 97, 196, 270, 599
TauI GCSGC 3 cut(s) 17, 143, 341
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 2 cut(s) 361, 767
TseI GCWGC 5 cut(s) 73, 143, 408, 871, 905
TspDTI ATGAA 3 cut(s) 577, 1046, 1230
TspGWI ACGGA 2 cut(s) 101, 464
TspRI CASTG 2 cut(s) 361, 767
VpaK11BI GGWCC 1 cut(s) 1085
XapI RAATTY 5 cut(s) 373, 431, 545, 601, 887
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XmaJI CCTAGG 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 4 cut(s) 105, 489, 893, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.