Rroxscaffold_3G00235910

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007408
Physical Location & Seq
Forward (+)
23130973 .. 23135185
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235910.1

Sequence Viewer

Length: 1161 bp
ATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAGCAGCGCGAGATTACTGAACGCCTTCGAGACCCTAGGGGCCTCCGCAAGGGAGGAGGAGGAGGCTCGGCGGCTGCCCCGCCCCGCAACTTTGGTGCCAATGGTGCTCGCCCCCGAGGCTTTACTCGACCTGCAGAGAGGGCTGATGTAGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTCGAGGATGGAGAAATTGTTGAGAGTGCTGAAGGAACCAAGGATGCGAAGAAGAAGGATTCTAGTGAGGCGGCGATAGCTGATCACAATGATAGGAGGTTGTCAAATTCCCAACAAGGTAGCTGGTTAAGGAGGGATGGCAGCCATGGAGCAGCCAAGAAGGAATTTGACATTCCAGCGACGGATCATGTTCCAAGGGTATTGCCCAAGCAAGAAGACCCTAGCTTGGTTAATAGGAACAAAAGAATGCTCGGGCAACTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAAACGTGCAACTCTCGGGAACAGAGGCTTTCATGCGGAGATCAAATTCTTTACAGAGAGCTGAAGAAAGAGCACGTGAAGAAAGTGAAAGACTTAGGATACAAGAGCGTGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGGCAGAGCCTCCAATATATTATCTACCCAAGAAACCATTGGATGAGGATGCTACCTTGACTGAGCAGCGAAAAGAAAAGGAATTTCTAGAATGGAAAGCAGCACGGCGGGAGGAACTGAGCGATTATCAGAAGCAGATTGGGGAGCAGTATCTAGCAAATGTTGAAAAGGAGTTGGAGAGGTGGCAAAATGCAAGGAAAGCAAGGAAAGCAAACAATGATACGATGAACTTACAGGAAACAATGGACAAAGAGTTGGACACACATAGGCTTGAGCATGGTCCCAAGAAAAGAAAGATCCCTGATGGAAGCAACAATGAGGATGAGGATGATGTGGAAGATATCAATGTAGTGGAGGATGACATGATGGATGATGTGTTGGATGTTGATGATAACAGTAGAATGGTTGATGAAACAGCTAAATCAGATGCAGGCAATGCCAGTCCAAATGCGGAGAACGTGGATTAG

Protein Analysis

386

Amino Acids

44.01

Weight (kDa)

5.36

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 165 - 231 1.3e-10 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 161
AccII CGCG 1 cut(s) 75
AclWI GGATC 3 cut(s) 228, 456, 985
AcsI RAATTY 5 cut(s) 370, 428, 542, 598, 776
AcuI CTGAAG 2 cut(s) 315, 636
AcvI CACGTG 1 cut(s) 629
AfiI CCNNNNNNNGG 2 cut(s) 115, 490
AgsI TTSAA 1 cut(s) 860
AluBI AGCT 7 cut(s) 58, 254, 344, 387, 489, 614, 1112
AluI AGCT 7 cut(s) 58, 254, 344, 387, 489, 614, 1112
Alw21I GWGCWC 4 cut(s) 39, 60, 175, 628
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 3 cut(s) 228, 456, 985
Ama87I CYCGRG 3 cut(s) 180, 515, 568
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 6 cut(s) 70, 140, 405, 416, 760, 794
ApoI RAATTY 5 cut(s) 370, 428, 542, 598, 776
Asp700I GAANNNNTTC 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 2 cut(s) 11, 75
AspS9I GGNCC 2 cut(s) 106, 974
AvaI CYCGRG 3 cut(s) 180, 515, 568
AvaII GGWCC 1 cut(s) 974
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 533
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 60
BbrPI CACGTG 1 cut(s) 629
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 4 cut(s) 39, 60, 175, 628
BbvI GCAGC 6 cut(s) 82, 127, 417, 428, 772, 806
BccI CCATC 4 cut(s) 266, 395, 992, 1054
BceAI ACGGC 1 cut(s) 815
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciVI GTATCC 1 cut(s) 645
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 5 cut(s) 102, 327, 486, 782, 848
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 205
BfuI GTATCC 1 cut(s) 645
BglI GCCNNNNNGGC 1 cut(s) 183
BglII AGATCT 1 cut(s) 691
BisI GCNGC 9 cut(s) 12, 71, 138, 141, 336, 406, 417, 761, 795
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 9 cut(s) 13, 72, 139, 142, 337, 407, 418, 762, 796
Bme18I GGWCC 1 cut(s) 974
BmeT110I CYCGRG 3 cut(s) 180, 515, 568
BmgT120I GGNCC 2 cut(s) 106, 974
BmiI GGNNCC 4 cut(s) 107, 163, 301, 976
BmsI GCATC 3 cut(s) 298, 733, 1111
BpiI GAAGAC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 986
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 101, 181, 303, 409, 458
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 490
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 1134
Bse3DI GCAATG 1 cut(s) 1135
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 5 cut(s) 101, 181, 303, 409, 458
BseLI CCNNNNNNNGG 2 cut(s) 115, 490
BseMI GCAATG 1 cut(s) 1135
BseMII CTCAG 2 cut(s) 747, 803
BseNI ACTGG 1 cut(s) 1134
BseRI GAGGAG 5 cut(s) 44, 135, 138, 141, 700
BseSI GKGCMC 1 cut(s) 533
BseXI GCAGC 6 cut(s) 82, 127, 417, 428, 772, 806
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 108
BshNI GGYRCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 4 cut(s) 39, 60, 175, 628
BsiHKCI CYCGRG 3 cut(s) 180, 515, 568
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 960
BslI CCNNNNNNNGG 2 cut(s) 115, 490
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 960
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 3 cut(s) 180, 515, 568
Bsp1286I GDGCHC 5 cut(s) 39, 60, 175, 533, 628
Bsp143I GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
Bsp19I CCATGG 1 cut(s) 409
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 748, 804
BspDI ATCGAT 1 cut(s) 51
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 4 cut(s) 107, 163, 301, 976
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 3 cut(s) 228, 456, 985
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 1135
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 1134
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 5 cut(s) 101, 181, 303, 409, 458
BssMI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
BssT1I CCWWGG 4 cut(s) 101, 303, 409, 458
Bst4CI ACNGT 1 cut(s) 1091
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 5 cut(s) 7, 68, 175, 242, 1126
BstDEI CTNAG 3 cut(s) 647, 756, 812
BstDSI CCRYGG 1 cut(s) 409
BstENI CCTNNNNNAGG 1 cut(s) 113
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 75
BstKTI GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 993
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
BstMWI GCNNNNNNNGC 9 cut(s) 11, 72, 170, 183, 206, 341, 893, 902, 1130
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 533
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 6 cut(s) 82, 127, 417, 428, 772, 806
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 2 cut(s) 691, 990
BstYI RGATCY 2 cut(s) 691, 990
Bsu15I ATCGAT 1 cut(s) 51
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 409
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 5 cut(s) 7, 68, 175, 242, 1126
CfoI GCGC 2 cut(s) 11, 75
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 106, 974
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 5 cut(s) 410, 452, 586, 971, 1057
DdeI CTNAG 3 cut(s) 647, 756, 812
DpnI GATC 7 cut(s) 50, 222, 348, 450, 595, 693, 992
DpnII GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 4 cut(s) 101, 303, 409, 458
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 90
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 1 cut(s) 974
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 315, 636
Eco72I CACGTG 1 cut(s) 629
Eco88I CYCGRG 3 cut(s) 180, 515, 568
EcoICRI GAGCTC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 106
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 4 cut(s) 101, 303, 409, 458
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 4 cut(s) 101, 303, 409, 458
FaeI CATG 5 cut(s) 413, 455, 589, 974, 1060
FaiI YATR 7 cut(s) 411, 453, 587, 712, 960, 972, 1058
FaqI GGGAC 1 cut(s) 960
FatI CATG 5 cut(s) 409, 451, 585, 970, 1056
FauI CCCGC 3 cut(s) 153, 158, 795
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 9 cut(s) 12, 71, 138, 141, 336, 406, 417, 761, 795
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 9 cut(s) 12, 71, 138, 141, 336, 406, 417, 761, 795
FspBI CTAG 5 cut(s) 102, 327, 486, 782, 848
GlaI GCGC 2 cut(s) 10, 74
GluI GCNGC 9 cut(s) 12, 71, 138, 141, 336, 406, 417, 761, 795
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 241
HhaI GCGC 2 cut(s) 11, 75
Hin1II CATG 5 cut(s) 413, 455, 589, 974, 1060
Hin6I GCGC 2 cut(s) 9, 73
HinP1I GCGC 2 cut(s) 9, 73
HinfI GANTC 1 cut(s) 323
HpaII CCGG 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 2 cut(s) 825, 1120
Hpy188III TCNNGA 4 cut(s) 95, 547, 570, 782
Hpy8I GTNNAC 1 cut(s) 665
Hpy99I CGWCG 1 cut(s) 448
HpyAV CCTTC 4 cut(s) 101, 290, 313, 418
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4IV ACGT 3 cut(s) 558, 628, 1152
HpyCH4V TGCA 4 cut(s) 200, 562, 887, 1124
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 72, 170, 183, 206, 341, 893, 902, 1130
HpyF3I CTNAG 3 cut(s) 647, 756, 812
HpySE526I ACGT 3 cut(s) 558, 628, 1152
Hsp92II CATG 5 cut(s) 413, 455, 589, 974, 1060
HspAI GCGC 2 cut(s) 9, 73
KroI GCCGGC 1 cut(s) 240
KroNI GCCGGC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
LmnI GCTCC 4 cut(s) 42, 63, 413, 838
Lsp1109I GCAGC 6 cut(s) 82, 127, 417, 428, 772, 806
LweI GCATC 3 cut(s) 298, 733, 1111
MaeI CTAG 5 cut(s) 102, 327, 486, 782, 848
MaeII ACGT 3 cut(s) 558, 628, 1152
MalI GATC 7 cut(s) 50, 222, 348, 450, 595, 693, 992
MboI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
MboII GAAGA 6 cut(s) 325, 328, 491, 629, 644, 1043
MflI RGATCY 2 cut(s) 691, 990
MhlI GDGCHC 5 cut(s) 39, 60, 175, 533, 628
MluCI AATT 7 cut(s) 279, 370, 428, 542, 598, 669, 776
MmeI TCCRAC 4 cut(s) 85, 849, 930, 1053
MroNI GCCGGC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 2 cut(s) 392, 495
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 516
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 9 cut(s) 11, 72, 170, 183, 206, 341, 893, 902, 1130
NaeI GCCGGC 1 cut(s) 242
NcoI CCATGG 1 cut(s) 409
NdeII GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
NgoMIV GCCGGC 1 cut(s) 240
NlaIII CATG 5 cut(s) 413, 455, 589, 974, 1060
NlaIV GGNNCC 4 cut(s) 107, 163, 301, 976
NmeAIII GCCGAG 1 cut(s) 113
PctI GAATGC 1 cut(s) 516
PdiI GCCGGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 90
PfeI GAWTC 1 cut(s) 323
PkrI GCNGC 9 cut(s) 13, 72, 139, 142, 337, 407, 418, 762, 796
PmaCI CACGTG 1 cut(s) 629
PmlI CACGTG 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 629
Psp124BI GAGCTC 1 cut(s) 60
PspCI CACGTG 1 cut(s) 629
PspN4I GGNNCC 4 cut(s) 107, 163, 301, 976
PspPI GGNCC 2 cut(s) 106, 974
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 691, 990
PvuII CAGCTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 392, 495
SatI GCNGC 9 cut(s) 12, 71, 138, 141, 336, 406, 417, 761, 795
Sau3AI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 990
Sau96I GGNCC 2 cut(s) 106, 974
SduI GDGCHC 5 cut(s) 39, 60, 175, 533, 628
SfaNI GCATC 3 cut(s) 298, 733, 1111
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 974
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
Sse9I AATT 7 cut(s) 279, 370, 428, 542, 598, 669, 776
SspMI CTAG 5 cut(s) 102, 327, 486, 782, 848
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 4 cut(s) 101, 303, 409, 458
TaaI ACNGT 1 cut(s) 1091
TaiI ACGT 3 cut(s) 561, 631, 1155
TaqI TCGA 4 cut(s) 51, 94, 193, 267
TasI AATT 7 cut(s) 279, 370, 428, 542, 598, 669, 776
TauI GCSGC 3 cut(s) 14, 140, 338
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 2 cut(s) 392, 495
Tru9I TTAA 2 cut(s) 392, 495
TseI GCWGC 6 cut(s) 70, 140, 405, 416, 760, 794
TspDTI ATGAA 3 cut(s) 574, 935, 1119
TspGWI ACGGA 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 974
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 5 cut(s) 370, 428, 542, 598, 776
XbaI TCTAGA 1 cut(s) 781
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 5 cut(s) 102, 327, 486, 782, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.