MD08G1227300.v1.1

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007408
Physical Location & Seq
Forward (+)
29362408 .. 29374853
12446 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1227300.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGGGAAGCGCCGCAGTTGAGAAAACGCAGGAGGAGCTCCGTAAGGAGATCGATGAACTCCAACGCCAACAGCGCGAGATTACGGAACGGCTTCGAGACCCTAGGGGACTCCGGAAGGGAGGCTTGGTGGCCGCTGGTCCTCGCAATTTTGCAGCCAATGGTGCTGGTGTTCGTCCGCGTGGATTTACTCGACCTGCAGATAGAGCTGATGCGGAGGATCAACGTCCTGCAAAACGCCGGCTTTCATCAGCGGTTGTCAAGGTCGAGGATGGAGAAATCACTGAAGCTGGTGAAGAGGTCAAGGATGTGAAGACGACTGATTCAGTTGTAGAAGCTACGGCTGATCAGAGTGATAGGAAGTTGGCAAATTCCCAACAAAGTGGCTGGTCAAGGAGGGAAGGGAATCATGGAGTAGCAAAGAAGGATTTTGAAATTCCAGCAGCAGATCATGTTCCGAGAGTATTGCCAAAAGAACAAGACCCCAGCTTGGTTAATAGGAACAAAAGAATGCTAGGACAACTTTTGGGGACTCTAGAGAAATTCAGGAAAGAGGACTTGCAACTTTCAGGGACAGAGGCATTCATGCGGAGATCAGATTCTTTACAGAGAGCTGAACAAAGAGCACGAGAAGAAAGTGAAAGACTTAGGCTACAAGAGCGTGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGACTCTTCGGGCACGCGTTAATGCCAAGACGGAAGAAAAGAAATTGGAACTTCTTTTTCTTCAGTGGAGTGAGCATAATAAAAGACTTGGGAATTTTATAAGGTATAAGGTTATTTTTAATTTTGGTCCAAAAAAAGTGTCCATATTCACATTTTATTGGGACAAAGATAAGTTTCTATTCATGCAAATATTAGTGTTGAAGCAACCTGATGACTGGATGTTTCCTGCGTTATTGTGGGGTTTTAGGACTAAAGCAGAGCCTCCAATATATTATCTGCCCAAGAAACCATTGGAAGAGGATGCTGCGCTAGCTGAACAGCGAAAAGAAAAGGAGTTTCTAGCATGGAAAGCTGCACGAAGAGAGGAACTGACCGAGTATCAGAAGCAGATTGGGGAGCAGTATATTGCCAATGTTGAAAAGGAGTTGGAGAGGTGGCAAAATGCAAGGAAACCAAGGAAAGCGAACAACGATGTGATGAACTTACAGGAAACAATGGACAAAGAGTTAGACACACACAGGCTTGAGCATGGTCCCAAGAAAAGAACCATCCCTGATGGAAGCAACAACGAAGATGAGGATGATGTGGAAGATATTAACGTAGCAGAGGACTACATGATTGATGATGTGCTTGACGTTGATGATAACAATCGAAGGGGCGATGAAATAGCTAAACCGGATGCAGGTAATACCAGTCCAAACGCAGATACTGTTGAGTAG

Protein Analysis

470

Amino Acids

54.32

Weight (kDa)

6.04

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 164 - 267 2.4e-16 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
Acc36I ACCTGC 2 cut(s) 202, 1364
AccII CGCG 3 cut(s) 75, 178, 708
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 8 cut(s) 12, 132, 176, 212, 251, 586, 671, 679
AclWI GGATC 1 cut(s) 225
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 4 cut(s) 367, 432, 539, 784
AcuI CTGAAG 2 cut(s) 303, 737
AfiI CCNNNNNNNGG 2 cut(s) 487, 1373
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 3 cut(s) 431, 892, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 9 cut(s) 37, 206, 287, 335, 486, 611, 1004, 1043, 1361
AluI AGCT 9 cut(s) 37, 206, 287, 335, 486, 611, 1004, 1043, 1361
Alw21I GWGCWC 2 cut(s) 39, 625
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 1 cut(s) 225
AlwNI CAGNNNCTG 1 cut(s) 1400
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 4 cut(s) 152, 440, 995, 1043
ApoI RAATTY 4 cut(s) 367, 432, 539, 784
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
Asp700I GAANNNNTTC 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 3 cut(s) 11, 75, 1000
AspS9I GGNCC 3 cut(s) 137, 818, 1223
AsuHPI GGTGA 1 cut(s) 302
AsuNHI GCTAGC 1 cut(s) 1000
AvaII GGWCC 3 cut(s) 137, 818, 1223
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 706
BanII GRGCYC 1 cut(s) 39
BauI CACGAG 1 cut(s) 624
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 2 cut(s) 39, 625
BbvI GCAGC 4 cut(s) 164, 452, 982, 1030
BccI CCATC 3 cut(s) 263, 1241, 1247
BceAI ACGGC 2 cut(s) 104, 354
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 5 cut(s) 102, 512, 533, 1001, 1031
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 2 cut(s) 202, 1364
BglII AGATCT 1 cut(s) 688
BisI GCNGC 6 cut(s) 12, 132, 153, 441, 996, 1044
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 6 cut(s) 13, 133, 154, 442, 997, 1045
Bme18I GGWCC 3 cut(s) 137, 818, 1223
BmgT120I GGNCC 3 cut(s) 137, 818, 1223
BmiI GGNNCC 1 cut(s) 1225
BmsI GCATC 3 cut(s) 199, 982, 1360
BmtI GCTAGC 1 cut(s) 1004
BpiI GAAGAC 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 1235
Bsa29I ATCGAT 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 1338
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 101, 1145
BsaWI WCCGGW 2 cut(s) 111, 1366
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 1373
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 2 cut(s) 911, 1383
Bse8I GATNNNNATC 1 cut(s) 1338
BseAI TCCGGA 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 101, 1145
BseGI GGATG 7 cut(s) 274, 310, 915, 997, 1239, 1276, 1375
BseJI GATNNNNATC 1 cut(s) 1338
BseLI CCNNNNNNNGG 2 cut(s) 487, 1373
BseNI ACTGG 2 cut(s) 911, 1383
BseRI GAGGAG 2 cut(s) 47, 697
BseSI GKGCMC 1 cut(s) 706
BseXI GCAGC 4 cut(s) 164, 452, 982, 1030
BseYI CCCAGC 1 cut(s) 482
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 3 cut(s) 75, 178, 708
BshFI GGCC 1 cut(s) 131
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 39, 625
BsiSI CCGG 3 cut(s) 112, 238, 1367
BslFI GGGAC 5 cut(s) 120, 541, 583, 866, 1209
BslI CCNNNNNNNGG 2 cut(s) 487, 1373
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 5 cut(s) 120, 541, 583, 866, 1209
BsmI GAATGC 2 cut(s) 513, 578
BsnI GGCC 1 cut(s) 131
Bso31I GGTCTC 1 cut(s) 90
Bsp1286I GDGCHC 3 cut(s) 39, 625, 706
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 6 cut(s) 48, 217, 343, 445, 590, 688
BspACI CCGC 8 cut(s) 12, 132, 176, 212, 251, 586, 671, 679
BspANI GGCC 1 cut(s) 131
BspDI ATCGAT 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 3 cut(s) 75, 178, 708
BspLI GGNNCC 1 cut(s) 1225
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 2 cut(s) 202, 1364
BspOI GCTAGC 1 cut(s) 1004
BspPI GGATC 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 2 cut(s) 911, 1383
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 101, 1145
BssMI GATC 6 cut(s) 48, 217, 343, 445, 590, 688
BssSI CACGAG 1 cut(s) 624
BssT1I CCWWGG 2 cut(s) 101, 1145
Bst2BI CACGAG 1 cut(s) 624
Bst4CI ACNGT 1 cut(s) 1402
Bst6I CTCTTC 4 cut(s) 288, 702, 981, 1045
BstC8I GCNNGC 3 cut(s) 239, 706, 1002
BstDEI CTNAG 1 cut(s) 644
BstF5I GGATG 7 cut(s) 274, 310, 915, 997, 1239, 1276, 1375
BstFNI CGCG 3 cut(s) 75, 178, 708
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 3 cut(s) 11, 75, 1000
BstKTI GATC 6 cut(s) 51, 220, 346, 448, 593, 691
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 6 cut(s) 48, 217, 343, 445, 590, 688
BstMWI GCNNNNNNNGC 7 cut(s) 34, 72, 161, 203, 655, 1001, 1040
BstSFI CTRYAG 1 cut(s) 195
BstSLI GKGCMC 1 cut(s) 706
BstUI CGCG 3 cut(s) 75, 178, 708
BstV1I GCAGC 4 cut(s) 164, 452, 982, 1030
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 688
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 51
BsuRI GGCC 1 cut(s) 131
BsuTUI ATCGAT 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 1365
BtsCI GGATG 7 cut(s) 274, 310, 915, 997, 1239, 1276, 1375
BtsIMutI CAGTG 2 cut(s) 279, 761
BveI ACCTGC 2 cut(s) 202, 1364
Cac8I GCNNGC 3 cut(s) 239, 706, 1002
CaiI CAGNNNCTG 1 cut(s) 1400
CfoI GCGC 3 cut(s) 11, 75, 1000
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 3 cut(s) 137, 818, 1223
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 7 cut(s) 407, 449, 583, 874, 1035, 1220, 1306
DdeI CTNAG 1 cut(s) 644
DpnI GATC 6 cut(s) 50, 219, 345, 447, 592, 690
DpnII GATC 6 cut(s) 48, 217, 343, 445, 590, 688
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 4 cut(s) 288, 702, 981, 1045
EarI CTCTTC 4 cut(s) 288, 702, 981, 1045
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 101, 1145
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 3 cut(s) 137, 818, 1223
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 2 cut(s) 303, 737
EcoICRI GAGCTC 1 cut(s) 37
EcoT14I CCWWGG 2 cut(s) 101, 1145
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 101, 1145
FaeI CATG 7 cut(s) 410, 452, 586, 877, 1038, 1223, 1309
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FaqI GGGAC 5 cut(s) 120, 541, 583, 866, 1209
FatI CATG 7 cut(s) 406, 448, 582, 873, 1034, 1219, 1305
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 6 cut(s) 12, 132, 153, 441, 996, 1044
FokI GGATG 7 cut(s) 281, 317, 922, 1004, 1226, 1283, 1382
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 6 cut(s) 12, 132, 153, 441, 996, 1044
FspBI CTAG 5 cut(s) 102, 512, 533, 1001, 1031
GlaI GCGC 3 cut(s) 10, 74, 999
GluI GCNGC 6 cut(s) 12, 132, 153, 441, 996, 1044
GsaI CCCAGC 1 cut(s) 486
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 1 cut(s) 131
HapII CCGG 3 cut(s) 112, 238, 1367
HhaI GCGC 3 cut(s) 11, 75, 1000
Hin1II CATG 7 cut(s) 410, 452, 586, 877, 1038, 1223, 1309
Hin6I GCGC 3 cut(s) 9, 73, 998
HinP1I GCGC 3 cut(s) 9, 73, 998
HinfI GANTC 6 cut(s) 108, 320, 403, 529, 596, 694
HpaII CCGG 3 cut(s) 112, 238, 1367
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 1 cut(s) 662
Hpy188I TCNGA 5 cut(s) 348, 456, 595, 693, 1074
Hpy188III TCNNGA 4 cut(s) 95, 112, 533, 544
Hpy8I GTNNAC 1 cut(s) 662
HpyAV CCTTC 4 cut(s) 109, 392, 415, 1338
HpyCH4III ACNGT 1 cut(s) 1402
HpyCH4IV ACGT 3 cut(s) 223, 1290, 1326
HpyCH4V TGCA 8 cut(s) 152, 197, 230, 559, 877, 1046, 1136, 1373
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 72, 161, 203, 655, 1001, 1040
HpyF3I CTNAG 1 cut(s) 644
HpySE526I ACGT 3 cut(s) 223, 1290, 1326
Hsp92II CATG 7 cut(s) 410, 452, 586, 877, 1038, 1223, 1309
HspAI GCGC 3 cut(s) 9, 73, 998
Kpn2I TCCGGA 1 cut(s) 111
KroI GCCGGC 1 cut(s) 237
KroNI GCCGGC 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 6 cut(s) 48, 217, 343, 445, 590, 688
LmnI GCTCC 3 cut(s) 34, 42, 1087
Lsp1109I GCAGC 4 cut(s) 164, 452, 982, 1030
LweI GCATC 3 cut(s) 199, 982, 1360
MaeI CTAG 5 cut(s) 102, 512, 533, 1001, 1031
MaeII ACGT 3 cut(s) 223, 1290, 1326
MalI GATC 6 cut(s) 50, 219, 345, 447, 592, 690
MboI GATC 6 cut(s) 48, 217, 343, 445, 590, 688
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 3 cut(s) 39, 625, 706
MluCI AATT 8 cut(s) 145, 367, 432, 539, 666, 734, 784, 811
MluI ACGCGT 1 cut(s) 706
MlyI GAGTC 3 cut(s) 102, 523, 688
MmeI TCCRAC 2 cut(s) 85, 1098
MroI TCCGGA 1 cut(s) 111
MroNI GCCGGC 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 4 cut(s) 492, 711, 810, 1287
MspA1I CMGCKG 2 cut(s) 134, 251
MspI CCGG 3 cut(s) 112, 238, 1367
Mva1269I GAATGC 2 cut(s) 513, 578
MvnI CGCG 3 cut(s) 75, 178, 708
MwoI GCNNNNNNNGC 7 cut(s) 34, 72, 161, 203, 655, 1001, 1040
NaeI GCCGGC 1 cut(s) 239
NdeII GATC 6 cut(s) 48, 217, 343, 445, 590, 688
NgoMIV GCCGGC 1 cut(s) 237
NheI GCTAGC 1 cut(s) 1000
NlaIII CATG 7 cut(s) 410, 452, 586, 877, 1038, 1223, 1309
NlaIV GGNNCC 1 cut(s) 1225
PctI GAATGC 2 cut(s) 513, 578
PdiI GCCGGC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 90
PfeI GAWTC 3 cut(s) 320, 403, 596
PkrI GCNGC 6 cut(s) 13, 133, 154, 442, 997, 1045
PleI GAGTC 3 cut(s) 102, 523, 688
PpsI GAGTC 3 cut(s) 102, 523, 688
PsiI TTATAA 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 482
PspN4I GGNNCC 1 cut(s) 1225
PspPI GGNCC 3 cut(s) 137, 818, 1223
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 1400
PsuI RGATCY 1 cut(s) 688
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 4 cut(s) 492, 711, 810, 1287
SatI GCNGC 6 cut(s) 12, 132, 153, 441, 996, 1044
Sau3AI GATC 6 cut(s) 48, 217, 343, 445, 590, 688
Sau96I GGNCC 3 cut(s) 137, 818, 1223
SchI GAGTC 3 cut(s) 102, 523, 688
SduI GDGCHC 3 cut(s) 39, 625, 706
SfaNI GCATC 3 cut(s) 199, 982, 1360
SfcI CTRYAG 1 cut(s) 195
SinI GGWCC 3 cut(s) 137, 818, 1223
SmlI CTYRAG 1 cut(s) 1214
SmoI CTYRAG 1 cut(s) 1214
Sse9I AATT 8 cut(s) 145, 367, 432, 539, 666, 734, 784, 811
SsiI CCGC 8 cut(s) 12, 132, 176, 212, 251, 586, 671, 679
SspI AATATT 1 cut(s) 882
SspMI CTAG 5 cut(s) 102, 512, 533, 1001, 1031
SstI GAGCTC 1 cut(s) 39
StyI CCWWGG 2 cut(s) 101, 1145
TaaI ACNGT 1 cut(s) 1402
TaiI ACGT 3 cut(s) 226, 1293, 1329
TaqI TCGA 5 cut(s) 51, 94, 190, 264, 1342
TaqII GACCGA 1 cut(s) 1079
TasI AATT 8 cut(s) 145, 367, 432, 539, 666, 734, 784, 811
TauI GCSGC 2 cut(s) 14, 134
TfiI GAWTC 3 cut(s) 320, 403, 596
Tru1I TTAA 4 cut(s) 492, 711, 810, 1287
Tru9I TTAA 4 cut(s) 492, 711, 810, 1287
TscAI CASTG 2 cut(s) 286, 761
TseI GCWGC 4 cut(s) 152, 440, 995, 1043
TspDTI ATGAA 6 cut(s) 69, 234, 571, 862, 1184, 1368
TspGWI ACGGA 3 cut(s) 29, 98, 737
TspRI CASTG 2 cut(s) 286, 761
VpaK11BI GGWCC 3 cut(s) 137, 818, 1223
XapI RAATTY 4 cut(s) 367, 432, 539, 784
XbaI TCTAGA 1 cut(s) 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 979
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 5 cut(s) 102, 512, 533, 1001, 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.