Rh7CG378200

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0007408
Physical Location & Seq
Reverse (-)
47587592 .. 47592321
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG378200.1

Sequence Viewer

Length: 1269 bp
ATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAGCAGCGCGAGATTACGGAACGCCTTCGAGACCCTAGGGGACTCCGCAAGGGAGGAGGAGGAGGCTCGGCGGCTGCCCCGCCCCGCAACTTTGGTGCCAATGGTGCTCGCCCCCGAGGCTTTACTCGACCTGCAGATAGGGCTGATGTGGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTCGAGGATGGAGAAATTGTTGAGAGTGCTGAAGGAACCAAGGATGCGAAGAACAAGGAGTCAAGCGAGGCAGCGATAGCTGATCACAGTGATAGGAGGTTGTCAAATTCCCAACAAGGTAGCTGGTTAAAGAGGGATGGCAGCCATGGAGCAGGCAAGAAGGAATTTGACATTCCAGCAACGGATCATGTTCCAAGGGTATTGCCAAAGCAAGAAGACCCTAGCTTGGTTAATAGGAACAAAAGAATGCTCGGGCAACTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAAACGTGCAACTCTCAGGAACAGAAGCTTTCATGCGGAGATCGAATTCTTTACAAAGAGCTGAAGAAAGAGCACGTGAAGAAAGTGAAAGACTTAGGATACAAGAGCGCGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGACTCTGCGGGCACGTGTTTCTGCCAAGACAGAAGAAAAAAAATTGGAACTGCTTTTTCTTCAGTGGAGTGAGCATCACAAGAAGCTTAGCAATTTTATAAGGACTAAGGCAGAGCCTCCAATATATTATCTACCCAAGAAACCATTGGATGAGGATGCTACCTTGGCTGAGCAGCGAAAAGAAAAGGAATTCCTAGAATGGAAAGCAGCACGAAGGGAGGAACTGAGCAATTATCAGAAGCAGATTGGGGAGCAGTATCTAGCAAATGTTGAAAAGGAGTTGGAGAGGTGGCAAAATGCAAGGAAAGCAAGGAAAGCAAACAATGATACGATGAACTTACAGGAAACAATGGACAAAGAATTGGACACACATAGGCTTGAGCATGGTCCCAAGAAAAGAAAGATCCCTGATGGAAGCAACAATGAGGATGAGGATGATGTGGAAGATATCAATGTAGTGGAGGATGACCTGATGGATGATGTGTTGGATGTTGATGATAACAGCAGAATGGTTGATGAAACAGCTAAATCAGATGCAGGCAATGCCAGTCCAAATGCGGAGAACATTGATTAG

Protein Analysis

422

Amino Acids

48.24

Weight (kDa)

5.9

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 165 - 292 5.3e-28 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
Acc36I ACCTGC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 161
AccII CGCG 2 cut(s) 75, 663
AclWI GGATC 3 cut(s) 228, 456, 1093
AcsI RAATTY 5 cut(s) 370, 428, 542, 598, 884
AcuI CTGAAG 3 cut(s) 315, 636, 740
AcvI CACGTG 2 cut(s) 629, 710
AfiI CCNNNNNNNGG 1 cut(s) 490
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 1 cut(s) 968
AluBI AGCT 9 cut(s) 58, 254, 344, 387, 489, 581, 614, 781, 1220
AluI AGCT 9 cut(s) 58, 254, 344, 387, 489, 581, 614, 781, 1220
Alw21I GWGCWC 4 cut(s) 39, 60, 175, 628
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 3 cut(s) 228, 456, 1093
Ama87I CYCGRG 2 cut(s) 180, 515
ApeKI GCWGC 6 cut(s) 70, 140, 335, 405, 868, 902
ApoI RAATTY 5 cut(s) 370, 428, 542, 598, 884
Asp700I GAANNNNTTC 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 3 cut(s) 11, 75, 663
AspS9I GGNCC 1 cut(s) 1082
AvaI CYCGRG 2 cut(s) 180, 515
AvaII GGWCC 1 cut(s) 1082
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 2 cut(s) 533, 709
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 60
BbrPI CACGTG 2 cut(s) 629, 710
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 4 cut(s) 39, 60, 175, 628
BbvI GCAGC 6 cut(s) 82, 127, 347, 417, 880, 914
BccI CCATC 4 cut(s) 266, 395, 1100, 1162
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 653, 687
BciVI GTATCC 1 cut(s) 645
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 4 cut(s) 102, 486, 890, 956
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 205
BfuI GTATCC 1 cut(s) 645
BglI GCCNNNNNGGC 1 cut(s) 183
BglII AGATCT 1 cut(s) 691
BisI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
BlnI CCTAGG 1 cut(s) 101
BlpI GCTNAGC 2 cut(s) 782, 864
BlsI GCNGC 8 cut(s) 13, 72, 139, 142, 337, 407, 870, 904
Bme18I GGWCC 1 cut(s) 1082
BmeT110I CYCGRG 2 cut(s) 180, 515
BmgT120I GGNCC 1 cut(s) 1082
BmiI GGNNCC 3 cut(s) 163, 301, 1084
BmsI GCATC 4 cut(s) 298, 778, 841, 1219
BpiI GAAGAC 1 cut(s) 486
Bpu1102I GCTNAGC 2 cut(s) 782, 864
BpuEI CTTGAG 1 cut(s) 1094
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 2 cut(s) 629, 710
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 6 cut(s) 101, 181, 303, 409, 458, 858
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 1242
Bse3DI GCAATG 1 cut(s) 1243
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 6 cut(s) 101, 181, 303, 409, 458, 858
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMI GCAATG 1 cut(s) 1243
BseMII CTCAG 3 cut(s) 582, 855, 911
BseNI ACTGG 1 cut(s) 1242
BseRI GAGGAG 5 cut(s) 44, 135, 138, 141, 700
BseSI GKGCMC 2 cut(s) 533, 709
BseXI GCAGC 6 cut(s) 82, 127, 347, 417, 880, 914
Bsh1236I CGCG 2 cut(s) 75, 663
BshNI GGYRCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 4 cut(s) 39, 60, 175, 628
BsiHKCI CYCGRG 2 cut(s) 180, 515
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 2 cut(s) 120, 1068
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 2 cut(s) 120, 1068
BsmI GAATGC 1 cut(s) 516
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 2 cut(s) 180, 515
Bsp1286I GDGCHC 6 cut(s) 39, 60, 175, 533, 628, 709
Bsp143I GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
Bsp1720I GCTNAGC 2 cut(s) 782, 864
Bsp19I CCATGG 1 cut(s) 409
BspCNI CTCAG 3 cut(s) 581, 856, 912
BspDI ATCGAT 1 cut(s) 51
BspFNI CGCG 2 cut(s) 75, 663
BspLI GGNNCC 3 cut(s) 163, 301, 1084
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 3 cut(s) 228, 456, 1093
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 1243
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 1242
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 6 cut(s) 101, 181, 303, 409, 458, 858
BssMI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
BssT1I CCWWGG 5 cut(s) 101, 303, 409, 458, 858
Bst4CI ACNGT 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstBAI YACGTR 2 cut(s) 629, 710
BstC8I GCNNGC 7 cut(s) 7, 68, 175, 242, 418, 705, 1234
BstDEI CTNAG 6 cut(s) 568, 647, 782, 801, 864, 920
BstDSI CCRYGG 1 cut(s) 409
BstFNI CGCG 2 cut(s) 75, 663
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 3 cut(s) 11, 75, 663
BstKTI GATC 7 cut(s) 51, 223, 349, 451, 596, 694, 1101
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 2 cut(s) 533, 709
BstUI CGCG 2 cut(s) 75, 663
BstV1I GCAGC 6 cut(s) 82, 127, 347, 417, 880, 914
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 2 cut(s) 691, 1098
BstYI RGATCY 2 cut(s) 691, 1098
Bsu15I ATCGAT 1 cut(s) 51
BsuI GTATCC 1 cut(s) 645
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 358, 764
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 7 cut(s) 7, 68, 175, 242, 418, 705, 1234
CfoI GCGC 3 cut(s) 11, 75, 663
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 1082
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 4 cut(s) 410, 452, 586, 1079
DdeI CTNAG 6 cut(s) 568, 647, 782, 801, 864, 920
DpnI GATC 7 cut(s) 50, 222, 348, 450, 595, 693, 1100
DpnII GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 5 cut(s) 101, 303, 409, 458, 858
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 90
Eco32I GATATC 1 cut(s) 1144
Eco47I GGWCC 1 cut(s) 1082
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 315, 636, 740
Eco72I CACGTG 2 cut(s) 629, 710
Eco88I CYCGRG 2 cut(s) 180, 515
EcoICRI GAGCTC 1 cut(s) 58
EcoRI GAATTC 2 cut(s) 598, 884
EcoRV GATATC 1 cut(s) 1144
EcoT14I CCWWGG 5 cut(s) 101, 303, 409, 458, 858
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 5 cut(s) 101, 303, 409, 458, 858
FaeI CATG 4 cut(s) 413, 455, 589, 1082
FaiI YATR 7 cut(s) 411, 453, 587, 794, 820, 1068, 1080
FaqI GGGAC 2 cut(s) 120, 1068
FatI CATG 4 cut(s) 409, 451, 585, 1078
FauI CCCGC 3 cut(s) 153, 158, 696
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
FspBI CTAG 4 cut(s) 102, 486, 890, 956
GlaI GCGC 3 cut(s) 10, 74, 662
GluI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
HaeII RGCGCY 1 cut(s) 12
HapII CCGG 1 cut(s) 241
HhaI GCGC 3 cut(s) 11, 75, 663
Hin1II CATG 4 cut(s) 413, 455, 589, 1082
Hin6I GCGC 3 cut(s) 9, 73, 661
HinP1I GCGC 3 cut(s) 9, 73, 661
HindIII AAGCTT 2 cut(s) 579, 779
HinfI GANTC 3 cut(s) 108, 323, 697
HpaII CCGG 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 696, 933, 1228
Hpy188III TCNNGA 3 cut(s) 95, 547, 570
HpyAV CCTTC 4 cut(s) 101, 290, 418, 903
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 3 cut(s) 558, 628, 709
HpyCH4V TGCA 4 cut(s) 200, 562, 995, 1232
HpyF3I CTNAG 6 cut(s) 568, 647, 782, 801, 864, 920
HpySE526I ACGT 3 cut(s) 558, 628, 709
Hsp92II CATG 4 cut(s) 413, 455, 589, 1082
HspAI GCGC 3 cut(s) 9, 73, 661
KroI GCCGGC 1 cut(s) 240
KroNI GCCGGC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
LmnI GCTCC 4 cut(s) 42, 63, 413, 946
Lsp1109I GCAGC 6 cut(s) 82, 127, 347, 417, 880, 914
LweI GCATC 4 cut(s) 298, 778, 841, 1219
MaeI CTAG 4 cut(s) 102, 486, 890, 956
MaeII ACGT 3 cut(s) 558, 628, 709
MalI GATC 7 cut(s) 50, 222, 348, 450, 595, 693, 1100
MboI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
MboII GAAGA 7 cut(s) 325, 491, 629, 644, 740, 746, 1151
MflI RGATCY 2 cut(s) 691, 1098
MhlI GDGCHC 6 cut(s) 39, 60, 175, 533, 628, 709
MlyI GAGTC 3 cut(s) 102, 332, 691
MmeI TCCRAC 3 cut(s) 85, 957, 1161
MroNI GCCGGC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 2 cut(s) 392, 495
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 516
MvnI CGCG 2 cut(s) 75, 663
NaeI GCCGGC 1 cut(s) 242
NcoI CCATGG 1 cut(s) 409
NdeII GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
NgoMIV GCCGGC 1 cut(s) 240
NlaIII CATG 4 cut(s) 413, 455, 589, 1082
NlaIV GGNNCC 3 cut(s) 163, 301, 1084
NmeAIII GCCGAG 1 cut(s) 113
PctI GAATGC 1 cut(s) 516
PdiI GCCGGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 90
PkrI GCNGC 8 cut(s) 13, 72, 139, 142, 337, 407, 870, 904
PleI GAGTC 3 cut(s) 102, 331, 691
PmaCI CACGTG 2 cut(s) 629, 710
PmlI CACGTG 2 cut(s) 629, 710
PpsI GAGTC 3 cut(s) 102, 331, 691
Ppu21I YACGTR 2 cut(s) 629, 710
PsiI TTATAA 1 cut(s) 794
Psp124BI GAGCTC 1 cut(s) 60
PspCI CACGTG 2 cut(s) 629, 710
PspN4I GGNNCC 3 cut(s) 163, 301, 1084
PspPI GGNCC 1 cut(s) 1082
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 691, 1098
PvuII CAGCTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 392, 495
SatI GCNGC 8 cut(s) 12, 71, 138, 141, 336, 406, 869, 903
Sau3AI GATC 7 cut(s) 48, 220, 346, 448, 593, 691, 1098
Sau96I GGNCC 1 cut(s) 1082
SchI GAGTC 3 cut(s) 102, 332, 691
SduI GDGCHC 6 cut(s) 39, 60, 175, 533, 628, 709
SfaNI GCATC 4 cut(s) 298, 778, 841, 1219
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
SspMI CTAG 4 cut(s) 102, 486, 890, 956
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 5 cut(s) 101, 303, 409, 458, 858
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 3 cut(s) 561, 631, 712
TaqI TCGA 5 cut(s) 51, 94, 193, 267, 596
TauI GCSGC 2 cut(s) 14, 140
Tru1I TTAA 2 cut(s) 392, 495
Tru9I TTAA 2 cut(s) 392, 495
TscAI CASTG 2 cut(s) 358, 764
TseI GCWGC 6 cut(s) 70, 140, 335, 405, 868, 902
TspDTI ATGAA 3 cut(s) 574, 1043, 1227
TspGWI ACGGA 2 cut(s) 98, 461
TspRI CASTG 2 cut(s) 358, 764
VpaK11BI GGWCC 1 cut(s) 1082
XapI RAATTY 5 cut(s) 370, 428, 542, 598, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 4 cut(s) 102, 486, 890, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.