Rw7G030580

pinin/SDK/memA/ protein conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007408
Physical Location & Seq
Reverse (-)
43390785 .. 43395641
4857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030580.1

Sequence Viewer

Length: 882 bp
ATGGCAAGCGCCGCAGTTGAGAAAACAGAGGAGGTGCTCCGCAAGGAGATCGATGAGCTCCAACGCCAGCAGCGCGAGATTACGGAACGCCTTCGAGACCCTAGGGGACTCCGCAAGGGAGGAGGAGGAGGAGGCTCGGCGGCTGCCCCGCCCCGCAACTTTGGTGCCAATGGTGCTCGCCCCCGAGGCTTTACTCGACCTGCAGATAGGGCTGATGTGGAGGATCAACCTCCTGCGAAACGCCGGCTTTCGTCAGCTGTTGTCAAGGTCGAGGATGGAGAAATTGTTGAGAGTGCTGAAGGAACCAAGGATGCGAAGAAGACGGAGTCAAGCGAGGCGGCGATAGCTGATCACAGTGATAGGAGGTTGTCAAATTCCCAACAAGGTAGCTGGTTAAGGAGGGATGGCAACCATGGAGCAGGCAAGAAGGAATTTGACATTCCAGCAACGGATCATGTTCCAAGGGTATTGCCAAAGCAAGAAGACCCTAGCTTGGTTAATAGGAACAAAAGAATGCTTGGGCAACTTTTGGGCACTTTAGAGAAATTCAGGAAAGAAAACGTGCAACTCTCAGGAACAGAAGCTTTCATGCGGAGATCGAATTCTTTACAAAGAGCTGAAGAAAGAGCACGTGAAGAAAGTGAAAGACTTAGGATACAAGAGCGCGAACAAATTGCGGAAAAGCGGAGGAGAGATCTGACTCTGCGGGCACGTGTTTCTGCCAAGACGGAAGAAAAAAAATTGGAACTGCTTTTTCTTCAGTGGAGTGAGCATCACAAGAAACTTAGCAATTTTATAAGGTTAATTTTTATTTTGGTCCAAAAAAAGTGTCCATATTCACATTTTATTGGGACGAAGATAAGTTTCTATTCATGTGAATAG

Protein Analysis

293

Amino Acids

33.33

Weight (kDa)

9.83

Isoelectric Point (pI)

62.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pinin_SDK_memA PF04696 166 - 267 2e-16 pinin/SDK/memA/ protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 2 cut(s) 75, 666
AclWI GGATC 2 cut(s) 231, 459
AcsI RAATTY 4 cut(s) 373, 431, 545, 601
AcuI CTGAAG 3 cut(s) 318, 639, 743
AcvI CACGTG 2 cut(s) 632, 713
AfiI CCNNNNNNNGG 1 cut(s) 493
AflIII ACRYGT 1 cut(s) 712
AluBI AGCT 7 cut(s) 58, 257, 347, 390, 492, 584, 617
AluI AGCT 7 cut(s) 58, 257, 347, 390, 492, 584, 617
Alw21I GWGCWC 4 cut(s) 39, 60, 178, 631
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 231, 459
Ama87I CYCGRG 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 70, 143
ApoI RAATTY 4 cut(s) 373, 431, 545, 601
ArsI GACNNNNNNTTYG 2 cut(s) 814, 846
Asp700I GAANNNNTTC 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 101
AspLEI GCGC 3 cut(s) 11, 75, 666
AspS9I GGNCC 1 cut(s) 817
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 1 cut(s) 817
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 2 cut(s) 536, 712
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 60
BbrPI CACGTG 2 cut(s) 632, 713
BbsI GAAGAC 2 cut(s) 326, 489
Bbv12I GWGCWC 4 cut(s) 39, 60, 178, 631
BbvI GCAGC 2 cut(s) 82, 130
BccI CCATC 2 cut(s) 269, 398
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 656, 690
BciVI GTATCC 1 cut(s) 648
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 2 cut(s) 102, 489
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 208
BfuI GTATCC 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 186
BglII AGATCT 1 cut(s) 694
BisI GCNGC 5 cut(s) 12, 71, 141, 144, 339
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 5 cut(s) 13, 72, 142, 145, 340
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 2 cut(s) 166, 304
BmsI GCATC 2 cut(s) 301, 781
BpiI GAAGAC 2 cut(s) 326, 489
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 2 cut(s) 632, 713
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 101, 184, 306, 412, 461
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 243
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 5 cut(s) 101, 184, 306, 412, 461
BseGI GGATG 3 cut(s) 280, 316, 409
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMII CTCAG 1 cut(s) 585
BseRI GAGGAG 6 cut(s) 44, 135, 138, 141, 144, 703
BseSI GKGCMC 2 cut(s) 536, 712
BseXI GCAGC 2 cut(s) 82, 130
Bsh1236I CGCG 2 cut(s) 75, 666
BshNI GGYRCC 1 cut(s) 164
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 4 cut(s) 39, 60, 178, 631
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 2 cut(s) 120, 865
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 2 cut(s) 120, 865
BsmI GAATGC 1 cut(s) 519
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 6 cut(s) 39, 60, 178, 536, 631, 712
Bsp143I GATC 6 cut(s) 48, 223, 349, 451, 596, 694
Bsp19I CCATGG 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 584
BspDI ATCGAT 1 cut(s) 51
BspFNI CGCG 2 cut(s) 75, 666
BspLI GGNNCC 2 cut(s) 166, 304
BspMAI CTGCAG 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 2 cut(s) 231, 459
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 243
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 5 cut(s) 101, 184, 306, 412, 461
BssMI GATC 6 cut(s) 48, 223, 349, 451, 596, 694
BssT1I CCWWGG 4 cut(s) 101, 306, 412, 461
Bst4CI ACNGT 1 cut(s) 356
BstBAI YACGTR 2 cut(s) 632, 713
BstC8I GCNNGC 6 cut(s) 7, 68, 178, 245, 421, 708
BstDEI CTNAG 3 cut(s) 571, 650, 785
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 3 cut(s) 280, 316, 409
BstFNI CGCG 2 cut(s) 75, 666
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 3 cut(s) 11, 75, 666
BstKTI GATC 6 cut(s) 51, 226, 352, 454, 599, 697
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 6 cut(s) 48, 223, 349, 451, 596, 694
BstMWI GCNNNNNNNGC 6 cut(s) 11, 72, 173, 186, 209, 344
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 2 cut(s) 536, 712
BstUI CGCG 2 cut(s) 75, 666
BstV1I GCAGC 2 cut(s) 82, 130
BstV2I GAAGAC 2 cut(s) 326, 489
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
Bsu15I ATCGAT 1 cut(s) 51
BsuI GTATCC 1 cut(s) 648
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 3 cut(s) 280, 316, 409
BtsIMutI CAGTG 2 cut(s) 361, 767
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 6 cut(s) 7, 68, 178, 245, 421, 708
CfoI GCGC 3 cut(s) 11, 75, 666
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 817
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 4 cut(s) 413, 455, 589, 873
DdeI CTNAG 3 cut(s) 571, 650, 785
DpnI GATC 6 cut(s) 50, 225, 351, 453, 598, 696
DpnII GATC 6 cut(s) 48, 223, 349, 451, 596, 694
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 4 cut(s) 101, 306, 412, 461
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 817
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 318, 639, 743
Eco72I CACGTG 2 cut(s) 632, 713
Eco88I CYCGRG 1 cut(s) 183
EcoICRI GAGCTC 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 601
EcoT14I CCWWGG 4 cut(s) 101, 306, 412, 461
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 4 cut(s) 101, 306, 412, 461
FaeI CATG 4 cut(s) 416, 458, 592, 876
FaiI YATR 6 cut(s) 414, 456, 590, 797, 835, 874
FaqI GGGAC 2 cut(s) 120, 865
FatI CATG 4 cut(s) 412, 454, 588, 872
FauI CCCGC 3 cut(s) 156, 161, 699
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 5 cut(s) 12, 71, 141, 144, 339
FokI GGATG 3 cut(s) 287, 323, 416
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 5 cut(s) 12, 71, 141, 144, 339
FspBI CTAG 2 cut(s) 102, 489
GlaI GCGC 3 cut(s) 10, 74, 665
GluI GCNGC 5 cut(s) 12, 71, 141, 144, 339
HaeII RGCGCY 1 cut(s) 12
HapII CCGG 1 cut(s) 244
HhaI GCGC 3 cut(s) 11, 75, 666
Hin1II CATG 4 cut(s) 416, 458, 592, 876
Hin6I GCGC 3 cut(s) 9, 73, 664
HinP1I GCGC 3 cut(s) 9, 73, 664
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 3 cut(s) 108, 326, 700
HpaII CCGG 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 699
Hpy188III TCNNGA 3 cut(s) 95, 550, 573
HpyAV CCTTC 3 cut(s) 101, 293, 421
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4IV ACGT 3 cut(s) 561, 631, 712
HpyCH4V TGCA 2 cut(s) 203, 565
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 72, 173, 186, 209, 344
HpyF3I CTNAG 3 cut(s) 571, 650, 785
HpySE526I ACGT 3 cut(s) 561, 631, 712
Hsp92II CATG 4 cut(s) 416, 458, 592, 876
HspAI GCGC 3 cut(s) 9, 73, 664
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 6 cut(s) 48, 223, 349, 451, 596, 694
LmnI GCTCC 3 cut(s) 42, 63, 416
LpnPI CCDG 9 cut(s) 80, 213, 246, 257, 376, 405, 456, 535, 558
Lsp1109I GCAGC 2 cut(s) 82, 130
LweI GCATC 2 cut(s) 301, 781
MaeI CTAG 2 cut(s) 102, 489
MaeII ACGT 3 cut(s) 561, 631, 712
MalI GATC 6 cut(s) 50, 225, 351, 453, 598, 696
MboI GATC 6 cut(s) 48, 223, 349, 451, 596, 694
MboII GAAGA 8 cut(s) 328, 331, 494, 632, 647, 743, 749, 868
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 6 cut(s) 39, 60, 178, 536, 631, 712
MluCI AATT 9 cut(s) 282, 373, 431, 545, 601, 672, 740, 790, 804
MlyI GAGTC 3 cut(s) 102, 335, 694
MmeI TCCRAC 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 3 cut(s) 395, 498, 803
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 244
Mva1269I GAATGC 1 cut(s) 519
MvnI CGCG 2 cut(s) 75, 666
MwoI GCNNNNNNNGC 6 cut(s) 11, 72, 173, 186, 209, 344
NaeI GCCGGC 1 cut(s) 245
NcoI CCATGG 1 cut(s) 412
NdeII GATC 6 cut(s) 48, 223, 349, 451, 596, 694
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 4 cut(s) 416, 458, 592, 876
NlaIV GGNNCC 2 cut(s) 166, 304
NmeAIII GCCGAG 1 cut(s) 116
PctI GAATGC 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 90
PflFI GACNNNGTC 1 cut(s) 325
PkrI GCNGC 5 cut(s) 13, 72, 142, 145, 340
PleI GAGTC 3 cut(s) 102, 334, 694
PmaCI CACGTG 2 cut(s) 632, 713
PmlI CACGTG 2 cut(s) 632, 713
PpsI GAGTC 3 cut(s) 102, 334, 694
Ppu21I YACGTR 2 cut(s) 632, 713
PsiI TTATAA 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 60
PspCI CACGTG 2 cut(s) 632, 713
PspN4I GGNNCC 2 cut(s) 166, 304
PspPI GGNCC 1 cut(s) 817
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 694
PsyI GACNNNGTC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 3 cut(s) 395, 498, 803
SatI GCNGC 5 cut(s) 12, 71, 141, 144, 339
Sau3AI GATC 6 cut(s) 48, 223, 349, 451, 596, 694
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 3 cut(s) 102, 335, 694
SduI GDGCHC 6 cut(s) 39, 60, 178, 536, 631, 712
SfaNI GCATC 2 cut(s) 301, 781
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 817
Sse9I AATT 9 cut(s) 282, 373, 431, 545, 601, 672, 740, 790, 804
SspMI CTAG 2 cut(s) 102, 489
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 4 cut(s) 101, 306, 412, 461
TaaI ACNGT 1 cut(s) 356
TaiI ACGT 3 cut(s) 564, 634, 715
TaqI TCGA 5 cut(s) 51, 94, 196, 270, 599
TasI AATT 9 cut(s) 282, 373, 431, 545, 601, 672, 740, 790, 804
TauI GCSGC 3 cut(s) 14, 143, 341
Tru1I TTAA 3 cut(s) 395, 498, 803
Tru9I TTAA 3 cut(s) 395, 498, 803
TscAI CASTG 2 cut(s) 361, 767
TseI GCWGC 2 cut(s) 70, 143
TspDTI ATGAA 2 cut(s) 577, 861
TspGWI ACGGA 4 cut(s) 98, 338, 464, 743
TspRI CASTG 2 cut(s) 361, 767
Tth111I GACNNNGTC 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 817
XapI RAATTY 4 cut(s) 373, 431, 545, 601
XmaJI CCTAGG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 2 cut(s) 102, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.