FvH4_5g32110

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004940
Physical Location & Seq
Forward (+)
23197417 .. 23199026
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32110.t1

Sequence Viewer

Length: 429 bp
ATGGGGAAGACACGTGGTATGGGAGCTGCCCGCAAGCTGAAGAACCACCGCAGGAGGCAAAGATGGGCTGACAAGTCATACAAGAAGTCTCATCTCGGAAACGAGTGGAAGAAGCCATTTGCTGGATCTTCCCATGCTAAAGGCATTGTTCTTGAGAAAATTGGTATTGAAGCTAAGCAGCCTAACTCTGCCATTAGAAAGTGTGCTCGAGTTCAGCTTATTAAGAATGGAAAGAAGATTGCTGCTTTTGTGCCAAATGATGGTTGCCTGAACTACATTGAAGAGAATGACGAAGTGCTTATTGCCGGATTTGGTCGTAAAGGACATGCTGTCGGTGATATTCCTGGAGTCCGGTTCAAGGTTGTGAAGGTTTCAGGCGTGTCACTTCTGGCTCTCTTCAAGGAGAAGAAGGAAAAGCCAAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

143

Amino Acids

15.78

Weight (kDa)

10.45

Isoelectric Point (pI)

24.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 38 - 141 3.7e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 122, 260
AciI CCGC 2 cut(s) 31, 49
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 14
AfiI CCNNNNNNNGG 3 cut(s) 122, 260, 358
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 4 cut(s) 170, 281, 358, 400
AhdI GACNNNNNGTC 1 cut(s) 329
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 4 cut(s) 26, 37, 173, 217
AluI AGCT 4 cut(s) 26, 37, 173, 217
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 207
ApeKI GCWGC 3 cut(s) 26, 178, 242
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 207
BbrPI CACGTG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 13, 190, 229
BccI CCATC 2 cut(s) 57, 254
BciT130I CCWGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 93
BglII AGATCT 1 cut(s) 422
BisI GCNGC 3 cut(s) 27, 179, 243
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 28, 180, 244
Bme1390I CCNGG 1 cut(s) 345
BmeRI GACNNNNNGTC 1 cut(s) 329
BmeT110I CYCGRG 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 14
BsaWI WCCGGW 1 cut(s) 351
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 260, 358
BseBI CCWGG 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 122, 260, 358
BseXI GCAGC 3 cut(s) 13, 190, 229
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 2 cut(s) 306, 352
BslI CCNNNNNNNGG 3 cut(s) 122, 260, 358
BsmAI GTCTC 1 cut(s) 93
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 125, 422
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 2 cut(s) 31, 49
BspPI GGATC 1 cut(s) 133
BssMI GATC 2 cut(s) 125, 422
Bst2UI CCWGG 1 cut(s) 345
Bst6I CTCTTC 2 cut(s) 276, 401
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 31, 35
BstDEI CTNAG 1 cut(s) 174
BstKTI GATC 2 cut(s) 128, 425
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 2 cut(s) 125, 422
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 343
BstV1I GCAGC 3 cut(s) 13, 190, 229
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 2 cut(s) 125, 422
BstYI RGATCY 2 cut(s) 125, 422
Cac8I GCNNGC 2 cut(s) 31, 35
CviAII CATG 2 cut(s) 134, 326
CviJI RGCY 9 cut(s) 26, 37, 68, 115, 173, 181, 217, 392, 418
CviKI_1 RGCY 9 cut(s) 26, 37, 68, 115, 173, 181, 217, 392, 418
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 127, 424
DpnII GATC 2 cut(s) 125, 422
DriI GACNNNNNGTC 1 cut(s) 329
Eam1104I CTCTTC 2 cut(s) 276, 401
Eam1105I GACNNNNNGTC 1 cut(s) 329
EarI CTCTTC 2 cut(s) 276, 401
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 343
FaeI CATG 2 cut(s) 137, 329
FaiI YATR 4 cut(s) 20, 79, 135, 327
FatI CATG 2 cut(s) 133, 325
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 3 cut(s) 27, 179, 243
Fsp4HI GCNGC 3 cut(s) 27, 179, 243
GluI GCNGC 3 cut(s) 27, 179, 243
GsuI CTGGAG 1 cut(s) 366
HapII CCGG 2 cut(s) 306, 352
Hin1II CATG 2 cut(s) 137, 329
HinfI GANTC 1 cut(s) 348
HpaII CCGG 2 cut(s) 306, 352
HphI GGTGA 1 cut(s) 347
Hpy188I TCNGA 1 cut(s) 98
Hpy188III TCNNGA 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 361, 403
HpyCH4IV ACGT 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 137, 329
Kzo9I GATC 2 cut(s) 125, 422
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 9 cut(s) 37, 108, 281, 319, 330, 357, 360, 365, 374
Lsp1109I GCAGC 3 cut(s) 13, 190, 229
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 381
MalI GATC 2 cut(s) 127, 424
MboI GATC 2 cut(s) 125, 422
MboII GAAGA 8 cut(s) 19, 52, 120, 121, 247, 293, 388, 418
MflI RGATCY 2 cut(s) 125, 422
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 1 cut(s) 159
MlyI GAGTC 1 cut(s) 357
MnlI CCTC 1 cut(s) 48
MseI TTAA 2 cut(s) 222, 427
MspI CCGG 2 cut(s) 306, 352
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
NdeII GATC 2 cut(s) 125, 422
NlaIII CATG 2 cut(s) 137, 329
NmuCI GTSAC 1 cut(s) 381
NspI RCATGY 1 cut(s) 329
PaeR7I CTCGAG 1 cut(s) 207
PflMI CCANNNNNTGG 2 cut(s) 122, 260
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 28, 180, 244
PleI GAGTC 1 cut(s) 356
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 1 cut(s) 356
Ppu21I YACGTR 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 343
PspCI CACGTG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 343
PspXI VCTCGAGB 1 cut(s) 207
PsuI RGATCY 2 cut(s) 125, 422
SaqAI TTAA 2 cut(s) 222, 427
SatI GCNGC 3 cut(s) 27, 179, 243
Sau3AI GATC 2 cut(s) 125, 422
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 208
SetI ASST 7 cut(s) 16, 28, 39, 175, 219, 363, 372
Sfr274I CTCGAG 1 cut(s) 207
SlaI CTCGAG 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 152, 207
SmoI CTYRAG 2 cut(s) 152, 207
Sse9I AATT 1 cut(s) 159
SsiI CCGC 2 cut(s) 31, 49
StyD4I CCNGG 1 cut(s) 343
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 208
TasI AATT 1 cut(s) 159
Tru1I TTAA 2 cut(s) 222, 427
Tru9I TTAA 2 cut(s) 222, 427
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 3 cut(s) 26, 178, 242
Tsp45I GTSAC 1 cut(s) 381
Van91I CCANNNNNTGG 2 cut(s) 122, 260
XceI RCATGY 1 cut(s) 329
XhoI CTCGAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.