Rh5DG426100

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004940
Physical Location & Seq
Forward (+)
68004425 .. 68006871
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG426100.1

Sequence Viewer

Length: 459 bp
ATGCCTTACTTAACACTGCTAGCCAATTTTTACAGGAAAACACGTGGAATGGGAGCAGCTCGCAAGCTGAAGGACCACCGCAGAAGGCAAAGGTGGGCTGACAAGTCATACAAGAAGTCACATCTTGGAAATGAGTGGAAGAAGCCATTTGCTGGATCTTCCCATGCTAAGGGCATTGTTCTTGAGAAGATTGGTATTGAGGCTAAGCAGCCTAACTCTGCCATTAGAAAGTGTGCTAGAGTCCAGCTTATCAAGAATGGGAAGAAGATTGCTGCCTTTGTGCCCAACGATGGTTGCTTGAACTACATTGAAGAGAATGACGAGGTGTTGATTGCTGGGTTTGGACGTAAAGGTCATGCTGTTGGTGATATTCCCGGAGTTAGGTTCAAGGTTGTGAAGGTCTCTGGGGTGTCTCTCTTGGCCTTGTTCAAGGAGAAGAAGGAGAAGCCAAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

152

Amino Acids

17.07

Weight (kDa)

10.34

Isoelectric Point (pI)

26.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 48 - 151 4.6e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 152
AciI CCGC 1 cut(s) 79
AclWI GGATC 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 89
AcvI CACGTG 1 cut(s) 44
AfiI CCNNNNNNNGG 4 cut(s) 152, 169, 290, 381
AflIII ACRYGT 1 cut(s) 41
AgsI TTSAA 4 cut(s) 301, 311, 388, 430
AluBI AGCT 3 cut(s) 59, 67, 247
AluI AGCT 3 cut(s) 59, 67, 247
Alw26I GTCTC 2 cut(s) 406, 417
AlwI GGATC 1 cut(s) 163
AoxI GGCC 1 cut(s) 420
ApeKI GCWGC 3 cut(s) 56, 208, 272
AspS9I GGNCC 1 cut(s) 73
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 377
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 44
BbvI GCAGC 3 cut(s) 68, 220, 259
BccI CCATC 1 cut(s) 284
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 406, 417
BfaI CTAG 2 cut(s) 20, 237
BglII AGATCT 1 cut(s) 452
BisI GCNGC 3 cut(s) 57, 209, 273
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 3 cut(s) 58, 210, 274
Bme1390I CCNGG 1 cut(s) 375
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 375
BmtI GCTAGC 1 cut(s) 23
Bpu10I CCTNAGC 1 cut(s) 168
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 406
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 169, 290, 381
BseLI CCNNNNNNNGG 4 cut(s) 152, 169, 290, 381
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 3 cut(s) 68, 220, 259
BseYI CCCAGC 1 cut(s) 335
BshFI GGCC 1 cut(s) 422
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 4 cut(s) 152, 169, 290, 381
BsmAI GTCTC 2 cut(s) 406, 417
BsnI GGCC 1 cut(s) 422
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 2 cut(s) 155, 452
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 1 cut(s) 79
BspANI GGCC 1 cut(s) 422
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 163
BspTNI GGTCTC 1 cut(s) 406
BssMI GATC 2 cut(s) 155, 452
Bst6I CTCTTC 1 cut(s) 306
BstBAI YACGTR 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 21, 61, 65
BstDEI CTNAG 2 cut(s) 168, 204
BstKTI GATC 2 cut(s) 158, 455
BstMAI GTCTC 2 cut(s) 406, 417
BstMBI GATC 2 cut(s) 155, 452
BstSCI CCNGG 1 cut(s) 373
BstSLI GKGCMC 1 cut(s) 285
BstV1I GCAGC 3 cut(s) 68, 220, 259
BstX2I RGATCY 2 cut(s) 155, 452
BstYI RGATCY 2 cut(s) 155, 452
BsuRI GGCC 1 cut(s) 422
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 3 cut(s) 21, 61, 65
Cfr13I GGNCC 1 cut(s) 73
CviAII CATG 2 cut(s) 164, 356
DdeI CTNAG 2 cut(s) 168, 204
DpnI GATC 2 cut(s) 157, 454
DpnII GATC 2 cut(s) 155, 452
DrdI GACNNNNNNGTC 1 cut(s) 351
DseDI GACNNNNNNGTC 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 89
Eco72I CACGTG 1 cut(s) 44
FaeI CATG 2 cut(s) 167, 359
FaiI YATR 3 cut(s) 109, 165, 357
FatI CATG 2 cut(s) 163, 355
Fnu4HI GCNGC 3 cut(s) 57, 209, 273
Fsp4HI GCNGC 3 cut(s) 57, 209, 273
FspBI CTAG 2 cut(s) 20, 237
GluI GCNGC 3 cut(s) 57, 209, 273
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 1 cut(s) 422
HapII CCGG 1 cut(s) 375
Hin1II CATG 2 cut(s) 167, 359
HinfI GANTC 1 cut(s) 240
HpaII CCGG 1 cut(s) 375
HphI GGTGA 1 cut(s) 377
Hpy188III TCNNGA 2 cut(s) 182, 253
HpyAV CCTTC 4 cut(s) 64, 78, 391, 433
HpyCH4IV ACGT 2 cut(s) 43, 346
HpyF3I CTNAG 2 cut(s) 168, 204
HpySE526I ACGT 2 cut(s) 43, 346
Hsp92II CATG 2 cut(s) 167, 359
Kzo9I GATC 2 cut(s) 155, 452
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 6 cut(s) 19, 138, 257, 321, 388, 390
Lsp1109I GCAGC 3 cut(s) 68, 220, 259
MaeI CTAG 2 cut(s) 20, 237
MaeII ACGT 2 cut(s) 43, 346
MaeIII GTNAC 1 cut(s) 117
MalI GATC 2 cut(s) 157, 454
MboI GATC 2 cut(s) 155, 452
MboII GAAGA 7 cut(s) 150, 151, 199, 274, 277, 323, 448
MflI RGATCY 2 cut(s) 155, 452
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 1 cut(s) 25
MlyI GAGTC 1 cut(s) 249
MnlI CCTC 2 cut(s) 193, 316
MseI TTAA 2 cut(s) 11, 457
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 375
NciI CCSGG 1 cut(s) 375
NdeII GATC 2 cut(s) 155, 452
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 2 cut(s) 167, 359
NmuCI GTSAC 1 cut(s) 117
PflMI CCANNNNNTGG 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 373
PkrI GCNGC 3 cut(s) 58, 210, 274
PleI GAGTC 1 cut(s) 248
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 1 cut(s) 248
Ppu21I YACGTR 1 cut(s) 44
PspCI CACGTG 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 335
PspPI GGNCC 1 cut(s) 73
PsuI RGATCY 2 cut(s) 155, 452
SaqAI TTAA 2 cut(s) 11, 457
SatI GCNGC 3 cut(s) 57, 209, 273
Sau3AI GATC 2 cut(s) 155, 452
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 1 cut(s) 249
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 1 cut(s) 285
SinI GGWCC 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 1 cut(s) 25
SsiI CCGC 1 cut(s) 79
SspMI CTAG 2 cut(s) 20, 237
StyD4I CCNGG 1 cut(s) 373
TaiI ACGT 2 cut(s) 46, 349
TasI AATT 1 cut(s) 25
Tru1I TTAA 2 cut(s) 11, 457
Tru9I TTAA 2 cut(s) 11, 457
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 3 cut(s) 56, 208, 272
Tsp45I GTSAC 1 cut(s) 117
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 73
XspI CTAG 2 cut(s) 20, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.