MD01G1131500.v1.1

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004940
Physical Location & Seq
Reverse (-)
24190744 .. 24192698
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1131500.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGGGAAAACACGTGGAATGGGAGCTGCTCGTAAGCTCAAGACTCACCGTAGGAATCAGAGGTGGGCCGACAAGTCATACAAGAAGTCCCACCTTGGAAATGAATGGAAAAAGCCATTTTCTGGTTCATCTCATGCCAAAGGCATTGTCCTTGAGAAGATTGGTATTGAGGCTAAGCAGCCTAACTCTGCTATTAGAAAGTGCGCTCGTGTTCAGCTGATCAAAAATGGGAAGAAGATTGCTGCTTTCGTACCCAACGATGGTTGCTTGAACTACATCGAGGAAAATGATGAGGTGTTGATTGCTGGATTCGGACGGAAGGGCCACGCTGTGGGAGATATTCCCGGAGTCAGGTTCAAGGTTGTGAAGGTATCCGGTGTCTCTCTCCTGGCCCTCTTCAAGGAGAAAAAGGAAAAGCCAAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

143

Amino Acids

15.74

Weight (kDa)

10.37

Isoelectric Point (pI)

19.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 38 - 141 3.1e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 122, 331
AcvI CACGTG 1 cut(s) 14
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 1 cut(s) 252
AfiI CCNNNNNNNGG 5 cut(s) 122, 260, 331, 351, 400
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 3 cut(s) 271, 358, 400
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 3 cut(s) 26, 37, 217
AluI AGCT 3 cut(s) 26, 37, 217
Alw26I GTCTC 1 cut(s) 385
AoxI GGCC 3 cut(s) 66, 322, 390
ApeKI GCWGC 3 cut(s) 26, 178, 242
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 3 cut(s) 66, 322, 391
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 38
BauI CACGAG 1 cut(s) 207
BbrPI CACGTG 1 cut(s) 14
BbvI GCAGC 3 cut(s) 13, 190, 229
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 389
BciVI GTATCC 1 cut(s) 382
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 385
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 3 cut(s) 27, 179, 243
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 28, 180, 244
Bme1390I CCNGG 2 cut(s) 345, 389
BmgT120I GGNCC 3 cut(s) 66, 322, 391
BmrFI CCNGG 2 cut(s) 345, 389
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 23, 173
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 94, 419
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 260, 331, 351, 400
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 94, 419
BseLI CCNNNNNNNGG 5 cut(s) 122, 260, 331, 351, 400
BseXI GCAGC 3 cut(s) 13, 190, 229
BshFI GGCC 3 cut(s) 68, 324, 392
BsiSI CCGG 2 cut(s) 345, 375
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 5 cut(s) 122, 260, 331, 351, 400
BsmAI GTCTC 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 3 cut(s) 68, 324, 392
Bsp143I GATC 1 cut(s) 219
Bsp1720I GCTNAGC 1 cut(s) 174
BspANI GGCC 3 cut(s) 68, 324, 392
BssECI CCNNGG 2 cut(s) 94, 419
BssMI GATC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 94, 419
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 50
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 14
BstDEI CTNAG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 398
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 1 cut(s) 219
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 2 cut(s) 343, 387
BstV1I GCAGC 3 cut(s) 13, 190, 229
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 3 cut(s) 68, 324, 392
CfoI GCGC 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 66, 322, 391
Csp6I GTAC 1 cut(s) 251
CviAII CATG 1 cut(s) 134
CviQI GTAC 1 cut(s) 251
DdeI CTNAG 1 cut(s) 174
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
DraIII CACNNNGTG 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 94, 419
Eco72I CACGTG 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 94, 419
ErhI CCWWGG 2 cut(s) 94, 419
FaeI CATG 1 cut(s) 137
FaiI YATR 2 cut(s) 79, 135
FaqI GGGAC 1 cut(s) 73
FatI CATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 27, 179, 243
Fsp4HI GCNGC 3 cut(s) 27, 179, 243
GlaI GCGC 1 cut(s) 205
GluI GCNGC 3 cut(s) 27, 179, 243
HaeIII GGCC 3 cut(s) 68, 324, 392
HapII CCGG 2 cut(s) 345, 375
HhaI GCGC 1 cut(s) 206
Hin1II CATG 1 cut(s) 137
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HinfI GANTC 4 cut(s) 43, 55, 309, 348
HpaII CCGG 2 cut(s) 345, 375
HphI GGTGA 1 cut(s) 38
Hpy188I TCNGA 2 cut(s) 60, 314
Hpy188III TCNNGA 1 cut(s) 40
HpyAV CCTTC 2 cut(s) 313, 361
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 1 cut(s) 137
HspAI GCGC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 7 cut(s) 108, 291, 337, 358, 374, 388, 401
Lsp1109I GCAGC 3 cut(s) 13, 190, 229
MaeII ACGT 1 cut(s) 13
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 4 cut(s) 169, 244, 247, 388
MlyI GAGTC 2 cut(s) 37, 357
MnlI CCTC 5 cut(s) 54, 163, 274, 286, 404
MseI TTAA 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 2 cut(s) 345, 375
MspR9I CCNGG 2 cut(s) 345, 389
MvaI CCWGG 1 cut(s) 389
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 219
NlaIII CATG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 2 cut(s) 55, 309
PflMI CCANNNNNTGG 2 cut(s) 122, 331
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 28, 180, 244
PleI GAGTC 2 cut(s) 37, 356
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 2 cut(s) 37, 356
Ppu21I YACGTR 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 387
PspCI CACGTG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 387
PspPI GGNCC 3 cut(s) 66, 322, 391
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 252
RsaNI GTAC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 427
SatI GCNGC 3 cut(s) 27, 179, 243
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 3 cut(s) 66, 322, 391
SchI GAGTC 2 cut(s) 37, 357
ScrFI CCNGG 2 cut(s) 345, 389
SmlI CTYRAG 2 cut(s) 38, 152
SmoI CTYRAG 2 cut(s) 38, 152
StyD4I CCNGG 2 cut(s) 343, 387
StyI CCWWGG 2 cut(s) 94, 419
TaaI ACNGT 1 cut(s) 50
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 279
TfiI GAWTC 2 cut(s) 55, 309
Tru1I TTAA 1 cut(s) 427
Tru9I TTAA 1 cut(s) 427
TseI GCWGC 3 cut(s) 26, 178, 242
TspDTI ATGAA 2 cut(s) 117, 117
TspGWI ACGGA 1 cut(s) 331
Van91I CCANNNNNTGG 2 cut(s) 122, 331
XagI CCTNNNNNAGG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.