Rroxscaffold_1G00019570

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004940
Physical Location & Seq
Reverse (-)
23921253 .. 23922851
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019570.1

Sequence Viewer

Length: 429 bp
ATGGGGAAAACACGTGGAATGGGAGCAGCTCGCAAGCTGAAGGACCACCGCAGAAGGCAAAGGTGGGCTGACAAGTCATACAAGAAGTCACATCTTGGAAATGAGTGGAAGAAGCCATTTGCTGGATCTTCCCATGCTAAGGGCATTGTTCTTGAGAAGATTGGTATTGAGGCTAAGCAGCCTAACTCTGCCATTAGAAAGTGTGCTAGAGTCCAGCTTATCAAGAATGGGAAGAAGATTGCTGCCTTTGTGCCCAACGATGGTTGCTTGAACTACATTGAAGAGAATGACGAGGTGTTGATTGCTGGGTTTGGACGTAAAGGTCATGCTGTTGGTGATATTCCCGGAGTTAGGTTCAAGGTTGTGAAGGTCTCTGGGGTGTCTCTCTTGGCCTTGTTCAAGGAGAAGAAGGAGAAGCCAAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

142

Amino Acids

15.78

Weight (kDa)

10.37

Isoelectric Point (pI)

24.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 38 - 141 3.7e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 321
AccB7I CCANNNNNTGG 1 cut(s) 122
AciI CCGC 1 cut(s) 49
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 14
AfiI CCNNNNNNNGG 4 cut(s) 122, 139, 260, 351
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 4 cut(s) 271, 281, 358, 400
AluBI AGCT 3 cut(s) 29, 37, 217
AluI AGCT 3 cut(s) 29, 37, 217
Alw26I GTCTC 2 cut(s) 376, 387
AlwI GGATC 1 cut(s) 133
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 26, 178, 242
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 347
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 255
BbrPI CACGTG 1 cut(s) 14
BbvI GCAGC 3 cut(s) 38, 190, 229
BccI CCATC 1 cut(s) 254
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 376, 387
BfaI CTAG 1 cut(s) 207
BglII AGATCT 1 cut(s) 422
BisI GCNGC 3 cut(s) 27, 179, 243
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 28, 180, 244
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 376
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 139, 260, 351
BseLI CCNNNNNNNGG 4 cut(s) 122, 139, 260, 351
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 3 cut(s) 38, 190, 229
BseYI CCCAGC 1 cut(s) 305
BshFI GGCC 1 cut(s) 392
BsiSI CCGG 1 cut(s) 345
BslI CCNNNNNNNGG 4 cut(s) 122, 139, 260, 351
BsmAI GTCTC 2 cut(s) 376, 387
BsnI GGCC 1 cut(s) 392
Bso31I GGTCTC 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 125, 422
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 392
BspPI GGATC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 376
BssMI GATC 2 cut(s) 125, 422
Bst6I CTCTTC 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 31, 35
BstDEI CTNAG 2 cut(s) 138, 174
BstKTI GATC 2 cut(s) 128, 425
BstMAI GTCTC 2 cut(s) 376, 387
BstMBI GATC 2 cut(s) 125, 422
BstSCI CCNGG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 255
BstV1I GCAGC 3 cut(s) 38, 190, 229
BstX2I RGATCY 2 cut(s) 125, 422
BstYI RGATCY 2 cut(s) 125, 422
BsuRI GGCC 1 cut(s) 392
Cac8I GCNNGC 2 cut(s) 31, 35
Cfr13I GGNCC 1 cut(s) 43
CviAII CATG 2 cut(s) 134, 326
CviJI RGCY 9 cut(s) 29, 37, 68, 115, 173, 181, 217, 392, 418
CviKI_1 RGCY 9 cut(s) 29, 37, 68, 115, 173, 181, 217, 392, 418
DdeI CTNAG 2 cut(s) 138, 174
DpnI GATC 2 cut(s) 127, 424
DpnII GATC 2 cut(s) 125, 422
DrdI GACNNNNNNGTC 1 cut(s) 321
DseDI GACNNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 14
FaeI CATG 2 cut(s) 137, 329
FaiI YATR 3 cut(s) 79, 135, 327
FatI CATG 2 cut(s) 133, 325
Fnu4HI GCNGC 3 cut(s) 27, 179, 243
Fsp4HI GCNGC 3 cut(s) 27, 179, 243
FspBI CTAG 1 cut(s) 207
GluI GCNGC 3 cut(s) 27, 179, 243
GsaI CCCAGC 1 cut(s) 309
HaeIII GGCC 1 cut(s) 392
HapII CCGG 1 cut(s) 345
Hin1II CATG 2 cut(s) 137, 329
HinfI GANTC 1 cut(s) 210
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 347
Hpy188III TCNNGA 2 cut(s) 152, 223
HpyAV CCTTC 4 cut(s) 34, 48, 361, 403
HpyCH4IV ACGT 2 cut(s) 13, 316
HpyF3I CTNAG 2 cut(s) 138, 174
HpySE526I ACGT 2 cut(s) 13, 316
Hsp92II CATG 2 cut(s) 137, 329
Kzo9I GATC 2 cut(s) 125, 422
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 5 cut(s) 108, 227, 291, 358, 360
Lsp1109I GCAGC 3 cut(s) 38, 190, 229
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 13, 316
MaeIII GTNAC 1 cut(s) 87
MalI GATC 2 cut(s) 127, 424
MboI GATC 2 cut(s) 125, 422
MboII GAAGA 7 cut(s) 120, 121, 169, 244, 247, 293, 418
MflI RGATCY 2 cut(s) 125, 422
MhlI GDGCHC 1 cut(s) 255
MlyI GAGTC 1 cut(s) 219
MnlI CCTC 2 cut(s) 163, 286
MseI TTAA 1 cut(s) 427
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 345
NciI CCSGG 1 cut(s) 345
NdeII GATC 2 cut(s) 125, 422
NlaIII CATG 2 cut(s) 137, 329
NmuCI GTSAC 1 cut(s) 87
PflMI CCANNNNNTGG 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 28, 180, 244
PleI GAGTC 1 cut(s) 218
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 1 cut(s) 218
Ppu21I YACGTR 1 cut(s) 14
PspCI CACGTG 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 305
PspPI GGNCC 1 cut(s) 43
PsuI RGATCY 2 cut(s) 125, 422
SaqAI TTAA 1 cut(s) 427
SatI GCNGC 3 cut(s) 27, 179, 243
Sau3AI GATC 2 cut(s) 125, 422
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 255
SinI GGWCC 1 cut(s) 43
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SsiI CCGC 1 cut(s) 49
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 343
TaiI ACGT 2 cut(s) 16, 319
Tru1I TTAA 1 cut(s) 427
Tru9I TTAA 1 cut(s) 427
TseFI GTSAC 1 cut(s) 87
TseI GCWGC 3 cut(s) 26, 178, 242
Tsp45I GTSAC 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 43
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.