MD07G1179600.v1.1

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004940
Physical Location & Seq
Reverse (-)
25904912 .. 25906865
1954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1179600.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGGGAAAACACGTGGAATGGGAGCTGCTCGTAAGCTCAAGACCCACCGCAGGAATCAGAGGTGGGCTGACAAGTCGTACAAGAAGTCCCACCTTGGGAATGAATGGAAAAAGCCATTTTCTGGTTCATCTCATGCCAAAGGCATTGTCCTAGAAAAGATTGGTATTGAGGCTAAGCAGCCTAACTCTGCTATTAGAAAGTGTGCTCGTGTTCAGCTGATCAAAAATGGAAAGAAGATTGCTGCTTTTGTACCCAACGATGGTTGCTTAAACTACATCGAGGAAAATGATGAGGTGTTGATTGCTGGATTCGGACGGAAGGGGCACGCTGTGGGAGATATTCCCGGAGTCAGGTTCAAGGTTGTGAAGGTGTCTGGTGTCTCTTTGTTGGCCCTCTTCAAGGAGAAGAAGGAAAAGCCAAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

143

Amino Acids

15.74

Weight (kDa)

10.37

Isoelectric Point (pI)

19.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 38 - 141 3.1e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AciI CCGC 1 cut(s) 49
AcvI CACGTG 1 cut(s) 14
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 2 cut(s) 80, 252
AfiI CCNNNNNNNGG 6 cut(s) 51, 96, 122, 260, 351, 400
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 2 cut(s) 358, 400
AluBI AGCT 3 cut(s) 26, 37, 217
AluI AGCT 3 cut(s) 26, 37, 217
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 385
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 26, 178, 242
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AspS9I GGNCC 1 cut(s) 391
AsuC2I CCSGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 327
BauI CACGAG 1 cut(s) 207
BbrPI CACGTG 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 13, 190, 229
BccI CCATC 1 cut(s) 254
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 385
BfaI CTAG 1 cut(s) 152
BisI GCNGC 3 cut(s) 27, 179, 243
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 28, 180, 244
Bme1390I CCNGG 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 391
BmrFI CCNGG 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 94, 419
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 96, 122, 260, 351, 400
BseDI CCNNGG 2 cut(s) 94, 419
BseLI CCNNNNNNNGG 6 cut(s) 51, 96, 122, 260, 351, 400
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 3 cut(s) 13, 190, 229
BshFI GGCC 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 6 cut(s) 51, 96, 122, 260, 351, 400
BsmAI GTCTC 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 208, 327
Bsp143I GATC 1 cut(s) 219
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 94, 419
BssMI GATC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 94, 419
Bst2BI CACGAG 1 cut(s) 207
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 398
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 327
BstV1I GCAGC 3 cut(s) 13, 190, 229
BsuRI GGCC 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 391
Csp6I GTAC 2 cut(s) 79, 251
CviAII CATG 1 cut(s) 134
CviJI RGCY 9 cut(s) 26, 37, 68, 115, 173, 181, 217, 392, 418
CviKI_1 RGCY 9 cut(s) 26, 37, 68, 115, 173, 181, 217, 392, 418
CviQI GTAC 2 cut(s) 79, 251
DdeI CTNAG 1 cut(s) 174
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
DraIII CACNNNGTG 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 94, 419
Eco72I CACGTG 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoT14I CCWWGG 2 cut(s) 94, 419
ErhI CCWWGG 2 cut(s) 94, 419
FaeI CATG 1 cut(s) 137
FaiI YATR 1 cut(s) 135
FaqI GGGAC 1 cut(s) 73
FatI CATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 27, 179, 243
Fsp4HI GCNGC 3 cut(s) 27, 179, 243
FspBI CTAG 1 cut(s) 152
GluI GCNGC 3 cut(s) 27, 179, 243
HaeIII GGCC 1 cut(s) 392
HapII CCGG 1 cut(s) 345
Hin1II CATG 1 cut(s) 137
HinfI GANTC 3 cut(s) 55, 309, 348
HpaII CCGG 1 cut(s) 345
Hpy188I TCNGA 2 cut(s) 60, 314
Hpy188III TCNNGA 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 313, 361, 403
HpyCH4IV ACGT 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 6 cut(s) 37, 108, 291, 337, 358, 360
Lsp1109I GCAGC 3 cut(s) 13, 190, 229
MaeI CTAG 1 cut(s) 152
MaeII ACGT 1 cut(s) 13
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 3 cut(s) 247, 388, 418
MhlI GDGCHC 2 cut(s) 208, 327
MlyI GAGTC 1 cut(s) 357
MnlI CCTC 5 cut(s) 54, 163, 274, 286, 404
MseI TTAA 2 cut(s) 269, 427
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 345
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 219
NlaIII CATG 1 cut(s) 137
PfeI GAWTC 2 cut(s) 55, 309
PflMI CCANNNNNTGG 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 28, 180, 244
PleI GAGTC 1 cut(s) 356
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 1 cut(s) 356
Ppu21I YACGTR 1 cut(s) 14
PspCI CACGTG 1 cut(s) 14
PspPI GGNCC 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 80, 252
RsaNI GTAC 2 cut(s) 79, 251
SaqAI TTAA 2 cut(s) 269, 427
SatI GCNGC 3 cut(s) 27, 179, 243
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 391
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 208, 327
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
SsiI CCGC 1 cut(s) 49
SspMI CTAG 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 94, 419
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 279
TfiI GAWTC 2 cut(s) 55, 309
Tru1I TTAA 2 cut(s) 269, 427
Tru9I TTAA 2 cut(s) 269, 427
TseI GCWGC 3 cut(s) 26, 178, 242
TspDTI ATGAA 2 cut(s) 117, 117
TspGWI ACGGA 1 cut(s) 331
Van91I CCANNNNNTGG 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 398
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.