MD11G1123500.v1.1

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004940
Physical Location & Seq
Reverse (-)
11354355 .. 11356239
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1123500.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGGGAAGACACGTGGAATGGGAGCAGCTCGCAAGCTGAAGACCCACCGTAGGAACCAAAGGTGGGCTGACAAGTCCTACAAGAAGTCCCATCTTGGAAATGAGTGGAAGAAACCCTTTTCTGGATCTTCCCACGCAAAAGGCATCGTTCTTGAGAAAATTGGTATTGAAGCTAAGCAGCCTAACTCTGCCATTAGAAAGTGTGCTCGAGTTCAGCTTATTAAGAATGGAAAGAAGATTGCTGCCTTTGTGCCAAATGATGGTTGCTTGAACTACATTGAAGAGAATGACGAGGTTCTTATTGCTGGATTTGGTCGTAAGGGCCATGCTGTTGGTGATATTCCCGGAGTTAGGTTCAAGGTCGTCAAGGTTTCAGGTGTGTCACTTCTTGCCCTCTTCAAGGAGAAGAAGGAGAAGCCAAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

143

Amino Acids

15.74

Weight (kDa)

10.37

Isoelectric Point (pI)

19.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 38 - 141 3.1e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 260
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 14
AfiI CCNNNNNNNGG 6 cut(s) 51, 64, 122, 260, 351, 400
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 5 cut(s) 170, 271, 281, 358, 400
AluBI AGCT 4 cut(s) 29, 37, 173, 217
AluI AGCT 4 cut(s) 29, 37, 173, 217
Alw21I GWGCWC 1 cut(s) 208
AlwI GGATC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 3 cut(s) 26, 178, 242
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 207
BbrPI CACGTG 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 14, 47
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 38, 190, 229
BccI CCATC 2 cut(s) 99, 254
BcnI CCSGG 1 cut(s) 345
BglII AGATCT 1 cut(s) 422
BisI GCNGC 3 cut(s) 27, 179, 243
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 28, 180, 244
Bme1390I CCNGG 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 153
BpiI GAAGAC 2 cut(s) 14, 47
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 14
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 64, 122, 260, 351, 400
BseLI CCNNNNNNNGG 6 cut(s) 51, 64, 122, 260, 351, 400
BseXI GCAGC 3 cut(s) 38, 190, 229
BshFI GGCC 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 6 cut(s) 51, 64, 122, 260, 351, 400
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 125, 422
Bsp1720I GCTNAGC 1 cut(s) 174
BspANI GGCC 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 56
BspPI GGATC 1 cut(s) 133
BssMI GATC 2 cut(s) 125, 422
Bst4CI ACNGT 1 cut(s) 50
Bst6I CTCTTC 2 cut(s) 276, 401
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 31, 35
BstDEI CTNAG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 398
BstKTI GATC 2 cut(s) 128, 425
BstMBI GATC 2 cut(s) 125, 422
BstSCI CCNGG 1 cut(s) 343
BstV1I GCAGC 3 cut(s) 38, 190, 229
BstV2I GAAGAC 2 cut(s) 14, 47
BstX2I RGATCY 2 cut(s) 125, 422
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 2 cut(s) 125, 422
BsuRI GGCC 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 31, 35
Cfr13I GGNCC 1 cut(s) 322
CviAII CATG 1 cut(s) 326
CviJI RGCY 8 cut(s) 29, 37, 68, 173, 181, 217, 324, 418
CviKI_1 RGCY 8 cut(s) 29, 37, 68, 173, 181, 217, 324, 418
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 127, 424
DpnII GATC 2 cut(s) 125, 422
Eam1104I CTCTTC 2 cut(s) 276, 401
EarI CTCTTC 2 cut(s) 276, 401
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 398
FaeI CATG 1 cut(s) 329
FaiI YATR 1 cut(s) 327
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 1 cut(s) 73
FatI CATG 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 27, 179, 243
Fsp4HI GCNGC 3 cut(s) 27, 179, 243
GluI GCNGC 3 cut(s) 27, 179, 243
HaeIII GGCC 1 cut(s) 324
HapII CCGG 1 cut(s) 345
Hin1II CATG 1 cut(s) 329
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 347
Hpy188III TCNNGA 2 cut(s) 123, 152
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 1 cut(s) 329
Kzo9I GATC 2 cut(s) 125, 422
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 4 cut(s) 108, 291, 358, 360
Lsp1109I GCAGC 3 cut(s) 38, 190, 229
LweI GCATC 1 cut(s) 153
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 381
MalI GATC 2 cut(s) 127, 424
MboI GATC 2 cut(s) 125, 422
MboII GAAGA 8 cut(s) 19, 52, 120, 121, 247, 293, 388, 418
MflI RGATCY 2 cut(s) 125, 422
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 1 cut(s) 159
MnlI CCTC 2 cut(s) 286, 404
MseI TTAA 2 cut(s) 222, 427
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 345
NciI CCSGG 1 cut(s) 345
NdeII GATC 2 cut(s) 125, 422
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 381
PaeR7I CTCGAG 1 cut(s) 207
PflMI CCANNNNNTGG 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 28, 180, 244
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
Ppu21I YACGTR 1 cut(s) 14
PspCI CACGTG 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 322
PspXI VCTCGAGB 1 cut(s) 207
PsuI RGATCY 2 cut(s) 125, 422
SaqAI TTAA 2 cut(s) 222, 427
SatI GCNGC 3 cut(s) 27, 179, 243
Sau3AI GATC 2 cut(s) 125, 422
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 207
SlaI CTCGAG 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 152, 207
SmoI CTYRAG 2 cut(s) 152, 207
Sse9I AATT 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 1 cut(s) 50
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 208
TasI AATT 1 cut(s) 159
Tru1I TTAA 2 cut(s) 222, 427
Tru9I TTAA 2 cut(s) 222, 427
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 3 cut(s) 26, 178, 242
Tsp45I GTSAC 1 cut(s) 381
Van91I CCANNNNNTGG 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.