MD03G1110100.v1.1

Belongs to the universal ribosomal protein uS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004940
Physical Location & Seq
Reverse (-)
9587685 .. 9589546
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1110100.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGGGAAGACACGGGGAATGGGAGCAGCTCGCAAGCTGAAGACCCACCGCAGGAACCAAAGGTGGGCTGACAAATCTTACAAGAAGTCCCATCTTGGAAATGAGTGGAAGAAACCATTTTCTGGATCTTCCCACGCAAAGGGCATTGTTCTTGAGAAAATTGGTATTGAAGCTAAGCAGCCTAACTCTGCCATTAGAAAGTGTGCTCGAGTTCAGCTTATTAAGAACGGAAAGAAGATTGCTGCCTTTGTGCCGAACGATGGTTGCTTGAACTACATTGAGGAAAATGACGAGGTTCTGATTGCTGGATTTGGTCGTAAAGGTCATGCTGTTGGTGATATTCCCGGAGTTAGGTTCAAGGTCGTGAAGGTTTCAGGCGTGTCCCTTCTGGCCCTCTTTAAGGAGAAGAAGGAGAAGCCAAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosom_S12_S23

Protein Analysis

143

Amino Acids

15.74

Weight (kDa)

10.37

Isoelectric Point (pI)

19.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 38 - 141 3.1e-48 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AciI CCGC 1 cut(s) 49
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 59
AfiI CCNNNNNNNGG 7 cut(s) 51, 64, 122, 139, 260, 351, 400
AgsI TTSAA 3 cut(s) 170, 271, 358
AluBI AGCT 4 cut(s) 29, 37, 173, 217
AluI AGCT 4 cut(s) 29, 37, 173, 217
Alw21I GWGCWC 1 cut(s) 208
AlwI GGATC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 26, 178, 242
AspS9I GGNCC 1 cut(s) 391
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 207
BbsI GAAGAC 2 cut(s) 14, 47
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 38, 190, 229
BccI CCATC 2 cut(s) 99, 254
BcnI CCSGG 1 cut(s) 345
BisI GCNGC 3 cut(s) 27, 179, 243
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 28, 180, 244
Bme1390I CCNGG 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 345
BpiI GAAGAC 2 cut(s) 14, 47
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 419
Bsc4I CCNNNNNNNGG 7 cut(s) 51, 64, 122, 139, 260, 351, 400
BseDI CCNNGG 1 cut(s) 419
BseLI CCNNNNNNNGG 7 cut(s) 51, 64, 122, 139, 260, 351, 400
BseXI GCAGC 3 cut(s) 38, 190, 229
BshFI GGCC 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 2 cut(s) 73, 367
BslI CCNNNNNNNGG 7 cut(s) 51, 64, 122, 139, 260, 351, 400
BsmFI GGGAC 2 cut(s) 73, 367
BsnI GGCC 1 cut(s) 392
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 1 cut(s) 125
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 392
BspLI GGNNCC 1 cut(s) 56
BspPI GGATC 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 31, 35
BstDEI CTNAG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 398
BstKTI GATC 1 cut(s) 128
BstMBI GATC 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 343
BstV1I GCAGC 3 cut(s) 38, 190, 229
BstV2I GAAGAC 2 cut(s) 14, 47
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BsuRI GGCC 1 cut(s) 392
Cac8I GCNNGC 2 cut(s) 31, 35
Cfr13I GGNCC 1 cut(s) 391
CviAII CATG 1 cut(s) 326
CviJI RGCY 8 cut(s) 29, 37, 68, 173, 181, 217, 392, 418
CviKI_1 RGCY 8 cut(s) 29, 37, 68, 173, 181, 217, 392, 418
DdeI CTNAG 1 cut(s) 174
DpnI GATC 1 cut(s) 127
DpnII GATC 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 1 cut(s) 329
FaiI YATR 1 cut(s) 327
FaqI GGGAC 2 cut(s) 73, 367
FatI CATG 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 27, 179, 243
Fsp4HI GCNGC 3 cut(s) 27, 179, 243
GluI GCNGC 3 cut(s) 27, 179, 243
HaeIII GGCC 1 cut(s) 392
HapII CCGG 1 cut(s) 345
Hin1II CATG 1 cut(s) 329
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 347
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 3 cut(s) 123, 152, 364
HpyAV CCTTC 3 cut(s) 361, 395, 403
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 1 cut(s) 329
Kzo9I GATC 1 cut(s) 125
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 6 cut(s) 37, 108, 291, 358, 360, 374
Lsp1109I GCAGC 3 cut(s) 38, 190, 229
MalI GATC 1 cut(s) 127
MboI GATC 1 cut(s) 125
MboII GAAGA 6 cut(s) 19, 52, 120, 121, 247, 418
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 1 cut(s) 159
MnlI CCTC 3 cut(s) 274, 286, 404
MseI TTAA 3 cut(s) 222, 399, 427
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 345
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 125
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 1 cut(s) 56
PaeR7I CTCGAG 1 cut(s) 207
PflMI CCANNNNNTGG 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 3 cut(s) 28, 180, 244
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 391
PspXI VCTCGAGB 1 cut(s) 207
PsuI RGATCY 1 cut(s) 125
SaqAI TTAA 3 cut(s) 222, 399, 427
SatI GCNGC 3 cut(s) 27, 179, 243
Sau3AI GATC 1 cut(s) 125
Sau96I GGNCC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 208
Sfr274I CTCGAG 1 cut(s) 207
SlaI CTCGAG 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 152, 207
SmoI CTYRAG 2 cut(s) 152, 207
Sse9I AATT 1 cut(s) 159
SsiI CCGC 1 cut(s) 49
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 1 cut(s) 419
TaqI TCGA 1 cut(s) 208
TasI AATT 1 cut(s) 159
Tru1I TTAA 3 cut(s) 222, 399, 427
Tru9I TTAA 3 cut(s) 222, 399, 427
TseI GCWGC 3 cut(s) 26, 178, 242
TspGWI ACGGA 1 cut(s) 243
Van91I CCANNNNNTGG 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.