FvH4_5g35252

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013683
Physical Location & Seq
Forward (+)
25817921 .. 25819237
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35252.t1

Sequence Viewer

Length: 1317 bp
ATGGCGTCATGTTCTTCTTCTTCTTCTTCTGGGTCTGGGTCTAGGGAGTTGATGAGGTTGGAGGAGCTTCCGTGCGGTGTTGTGCGAACTTTTAAGCCTACCGATGCACAGCTTGTGGGTCATTATCTGTATGGTAAGATCAGGATGATGGGTCAAGTTGGAAATGGCAAACAGGCGTTTCCTGAATTGGATGTGTATGGAATAACTGGAATGGAGCCTTGGCTTATTTGGGATTGCTTAGAGGCAATTAGATTTCCTCACCAGGACTTCTCGTATTTCTTTACAAGAGTGAAGAGGTTGTCTGCCAAGGGCAAGGGAAAGCGGTGCTCTCGGACAGTTGGGTCTGGGGGAAAATGGAGTGAAAACGAGTCTTCCAAGGAGGTATATGTCGATGGTTTTCAAGAGCCCTTTGGGAGAATGAGGAAGCTCCGATACGAAAACAGTGACAAAGAATTTGAGCATCACGGTGCTTGGATGATAGAGGAGTTTTCAATTGAACATCCTGGTGTAGTTTCCTCGGATTTGGTGCTGTGCCGAATGAAAAGGAATGGAAAGAGCAACAACAAAAAGAGGAAGGCTGTAGAGGATGAGGAGGTGAATGCTAAAAGGGCCAAGTGTTTCCAAGCAGAAAAACAAGTAATTTCTGATTCTCATCATGAGCAGCAACAGCAGTGGCAGCTCTCCGACGTTCATGTTCAGCAACAGCAGTGTGGTGACAATGTTGTTGATCGAGCTGGTCTGGAACAAGGTAGTACTAGTGCCCAGCAGCAGCAGCAGATTCCTGCTCCTCATTTCGATAACAACTACAACTGTACTGCCGATGATGTAATTGTTTCTCAAAATGAGAGTGAACATGCAGTGTTAGAGGAAGATACCGGTTGGTGGCTTGAAAACATCATTGCATGGGATGAAGATGTAGACCCTTCCCTCTATGAAAACCACAGTTCTGCTGCTGATGATCTAACAGTCTCTGTAAATGAATCAACTGCTTTACAGTTAGAGGAGGAGGGCACTGATCATAATGGCTCTGTATCGGAGGGAGCAAGAGAGGAGACCCTACCCCCTGCCCTCTTAGAACAACATACATCTTCATCACCATTGATGTCGCAGCAGCAGCACCTGACAGATGATGATCAAGCATTTGCTTTTGCTTGTGATAATGATACCATTCCTATGGATGGTGTCTCCTGCTTTCAATTTGACGACAGTAGCAACTTCGACTTCCACTTTGAAAATCAGTACTTGTCTGAGGAAGCTACTCTACCAGTCAGTACCTCATCAGGAGATGACAATGGCCTACCACGTTTAGGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

439

Amino Acids

49.18

Weight (kDa)

4.69

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 30 - 165 2.8e-15 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 918
AciI CCGC 2 cut(s) 75, 322
AcsI RAATTY 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 754, 814, 1241, 1273
AfiI CCNNNNNNNGG 3 cut(s) 882, 1178, 1307
AgeI ACCGGT 1 cut(s) 875
AgsI TTSAA 6 cut(s) 401, 492, 497, 890, 1196, 1232
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 2 cut(s) 261, 502
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AluBI AGCT 6 cut(s) 67, 112, 427, 679, 734, 1256
AluI AGCT 6 cut(s) 67, 112, 427, 679, 734, 1256
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 3 cut(s) 973, 1046, 1189
AlwNI CAGNNNCTG 2 cut(s) 971, 1120
AoxI GGCC 2 cut(s) 609, 1294
ApeKI GCWGC 9 cut(s) 661, 676, 766, 769, 772, 950, 1108, 1111, 1114
ApoI RAATTY 1 cut(s) 452
ArsI GACNNNNNNTTYG 4 cut(s) 437, 469, 1211, 1243
AsiGI ACCGGT 1 cut(s) 875
AspS9I GGNCC 1 cut(s) 609
AsuHPI GGTGA 4 cut(s) 251, 607, 725, 1086
BaeGI GKGCMC 2 cut(s) 763, 1013
BanII GRGCYC 1 cut(s) 408
BarI GAAGNNNNNNTAC 4 cut(s) 416, 448, 1245, 1277
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 9 cut(s) 673, 688, 778, 781, 784, 937, 1120, 1123, 1126
BccI CCATC 3 cut(s) 142, 386, 1172
BciT130I CCWGG 2 cut(s) 263, 504
BclI TGATCA 2 cut(s) 1015, 1132
BcoDI GTCTC 3 cut(s) 973, 1046, 1189
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 3 cut(s) 42, 756, 1315
BfmI CTRYAG 1 cut(s) 579
BisI GCNGC 9 cut(s) 662, 677, 767, 770, 773, 951, 1109, 1112, 1115
BlsI GCNGC 9 cut(s) 663, 678, 768, 771, 774, 952, 1110, 1113, 1116
BmcAI AGTACT 2 cut(s) 754, 1241
Bme1390I CCNGG 2 cut(s) 263, 504
BmgT120I GGNCC 1 cut(s) 609
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 2 cut(s) 263, 504
BmsI GCATC 2 cut(s) 94, 469
BpiI GAAGAC 1 cut(s) 363
BsaBI GATNNNNATC 2 cut(s) 651, 1131
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1046
BsaJI CCNNGG 4 cut(s) 218, 306, 375, 516
BsaWI WCCGGW 1 cut(s) 875
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 3 cut(s) 882, 1178, 1307
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 2 cut(s) 211, 1265
Bse3DI GCAATG 1 cut(s) 897
Bse8I GATNNNNATC 2 cut(s) 651, 1131
BseBI CCWGG 2 cut(s) 263, 504
BseDI CCNNGG 4 cut(s) 218, 306, 375, 516
BseGI GGATG 7 cut(s) 150, 196, 480, 499, 592, 913, 1183
BseJI GATNNNNATC 2 cut(s) 651, 1131
BseLI CCNNNNNNNGG 3 cut(s) 882, 1178, 1307
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 1 cut(s) 1239
BseNI ACTGG 2 cut(s) 211, 1265
BseRI GAGGAG 7 cut(s) 77, 497, 605, 777, 1016, 1019, 1064
BseSI GKGCMC 2 cut(s) 763, 1013
BseXI GCAGC 9 cut(s) 673, 688, 778, 781, 784, 937, 1120, 1123, 1126
BseYI CCCAGC 1 cut(s) 762
BshFI GGCC 2 cut(s) 611, 1296
BshTI ACCGGT 1 cut(s) 875
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 1 cut(s) 876
BslI CCNNNNNNNGG 3 cut(s) 882, 1178, 1307
BsmAI GTCTC 3 cut(s) 973, 1046, 1189
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 2 cut(s) 611, 1296
Bso31I GGTCTC 1 cut(s) 1046
Bsp1286I GDGCHC 4 cut(s) 329, 408, 763, 1013
Bsp143I GATC 5 cut(s) 138, 727, 958, 1015, 1132
BspACI CCGC 2 cut(s) 75, 322
BspANI GGCC 2 cut(s) 611, 1296
BspCNI CTCAG 1 cut(s) 1240
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 1046
BsrDI GCAATG 1 cut(s) 897
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 2 cut(s) 211, 1265
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 4 cut(s) 218, 306, 375, 516
BssMI GATC 5 cut(s) 138, 727, 958, 1015, 1132
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 218, 306, 375
Bst2UI CCWGG 2 cut(s) 263, 504
Bst4CI ACNGT 8 cut(s) 337, 443, 467, 812, 944, 967, 996, 1208
Bst6I CTCTTC 1 cut(s) 287
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 3 cut(s) 238, 1072, 1248
BstF5I GGATG 7 cut(s) 150, 196, 480, 499, 592, 913, 1183
BstKTI GATC 5 cut(s) 141, 730, 961, 1018, 1135
BstMAI GTCTC 3 cut(s) 973, 1046, 1189
BstMBI GATC 5 cut(s) 138, 727, 958, 1015, 1132
BstMWI GCNNNNNNNGC 5 cut(s) 608, 667, 676, 772, 1114
BstNI CCWGG 2 cut(s) 263, 504
BstNSI RCATGY 1 cut(s) 857
BstSCI CCNGG 2 cut(s) 261, 502
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 2 cut(s) 763, 1013
BstV1I GCAGC 9 cut(s) 673, 688, 778, 781, 784, 937, 1120, 1123, 1126
BstV2I GAAGAC 1 cut(s) 363
BstXI CCANNNNNNTGG 1 cut(s) 1174
BsuRI GGCC 2 cut(s) 611, 1296
BtsCI GGATG 7 cut(s) 150, 196, 480, 499, 592, 913, 1183
BtsI GCAGTG 3 cut(s) 677, 713, 864
BtsIMutI CAGTG 5 cut(s) 448, 677, 713, 864, 1011
CaiI CAGNNNCTG 2 cut(s) 971, 1120
CciI TCATGA 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 1 cut(s) 609
Csp6I GTAC 4 cut(s) 753, 813, 1240, 1272
CspAI ACCGGT 1 cut(s) 875
CspCI CAANNNNNGTGG 2 cut(s) 653, 688
CviAII CATG 5 cut(s) 9, 656, 692, 854, 903
CviQI GTAC 4 cut(s) 753, 813, 1240, 1272
DdeI CTNAG 3 cut(s) 238, 1072, 1248
DpnI GATC 5 cut(s) 140, 729, 960, 1017, 1134
DpnII GATC 5 cut(s) 138, 727, 958, 1015, 1132
DrdI GACNNNNNNGTC 1 cut(s) 340
DseDI GACNNNNNNGTC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
Eco130I CCWWGG 3 cut(s) 218, 306, 375
Eco24I GRGCYC 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 1046
EcoRII CCWGG 2 cut(s) 261, 502
EcoT14I CCWWGG 3 cut(s) 218, 306, 375
EcoT38I GRGCYC 1 cut(s) 408
ErhI CCWWGG 3 cut(s) 218, 306, 375
FaeI CATG 5 cut(s) 12, 659, 695, 857, 906
FatI CATG 5 cut(s) 8, 655, 691, 853, 902
FbaI TGATCA 2 cut(s) 1015, 1132
FblI GTMKAC 1 cut(s) 918
Fnu4HI GCNGC 9 cut(s) 662, 677, 767, 770, 773, 951, 1109, 1112, 1115
FokI GGATG 7 cut(s) 157, 203, 486, 487, 599, 920, 1190
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 9 cut(s) 662, 677, 767, 770, 773, 951, 1109, 1112, 1115
FspBI CTAG 3 cut(s) 42, 756, 1315
GluI GCNGC 9 cut(s) 662, 677, 767, 770, 773, 951, 1109, 1112, 1115
GsaI CCCAGC 1 cut(s) 766
HaeIII GGCC 2 cut(s) 611, 1296
HapII CCGG 1 cut(s) 876
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 12, 659, 695, 857, 906
HinfI GANTC 5 cut(s) 368, 647, 778, 980, 1311
HpaII CCGG 1 cut(s) 876
HphI GGTGA 4 cut(s) 251, 607, 725, 1086
Hpy166II GTNNAC 2 cut(s) 851, 919
Hpy188I TCNGA 7 cut(s) 333, 431, 520, 646, 685, 1036, 1249
Hpy188III TCNNGA 6 cut(s) 142, 182, 401, 656, 740, 1281
Hpy8I GTNNAC 2 cut(s) 851, 919
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 2 cut(s) 568, 933
HpyCH4III ACNGT 8 cut(s) 337, 443, 467, 812, 944, 967, 996, 1208
HpyCH4IV ACGT 2 cut(s) 687, 1303
HpyCH4V TGCA 3 cut(s) 107, 857, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 667, 676, 772, 1114
HpyF3I CTNAG 3 cut(s) 238, 1072, 1248
HpySE526I ACGT 2 cut(s) 687, 1303
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 12, 659, 695, 857, 906
Ksp22I TGATCA 2 cut(s) 1015, 1132
Kzo9I GATC 5 cut(s) 138, 727, 958, 1015, 1132
LmnI GCTCC 5 cut(s) 64, 214, 432, 790, 1040
Lsp1109I GCAGC 9 cut(s) 673, 688, 778, 781, 784, 937, 1120, 1123, 1126
LweI GCATC 2 cut(s) 94, 469
MaeI CTAG 3 cut(s) 42, 756, 1315
MaeII ACGT 2 cut(s) 687, 1303
MaeIII GTNAC 2 cut(s) 443, 713
MalI GATC 5 cut(s) 140, 729, 960, 1017, 1134
MboI GATC 5 cut(s) 138, 727, 958, 1015, 1132
MfeI CAATTG 1 cut(s) 492
MhlI GDGCHC 4 cut(s) 329, 408, 763, 1013
MluCI AATT 7 cut(s) 185, 246, 452, 492, 639, 828, 1196
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 3 cut(s) 39, 139, 708
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 3 cut(s) 465, 504, 1172
MspI CCGG 1 cut(s) 876
MspR9I CCNGG 2 cut(s) 263, 504
MunI CAATTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 263, 504
MwoI GCNNNNNNNGC 5 cut(s) 608, 667, 676, 772, 1114
NdeII GATC 5 cut(s) 138, 727, 958, 1015, 1132
NlaIII CATG 5 cut(s) 12, 659, 695, 857, 906
NlaIV GGNNCC 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 443, 713
NspI RCATGY 1 cut(s) 857
PagI TCATGA 1 cut(s) 655
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 4 cut(s) 647, 778, 980, 1311
PinAI ACCGGT 1 cut(s) 875
PkrI GCNGC 9 cut(s) 663, 678, 768, 771, 774, 952, 1110, 1113, 1116
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 2 cut(s) 261, 502
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 2 cut(s) 261, 502
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 1 cut(s) 609
PstNI CAGNNNCTG 2 cut(s) 971, 1120
RsaI GTAC 4 cut(s) 754, 814, 1241, 1273
RsaNI GTAC 4 cut(s) 753, 813, 1240, 1272
RseI CAYNNNNRTG 3 cut(s) 465, 504, 1172
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 9 cut(s) 662, 677, 767, 770, 773, 951, 1109, 1112, 1115
Sau3AI GATC 5 cut(s) 138, 727, 958, 1015, 1132
Sau96I GGNCC 1 cut(s) 609
ScaI AGTACT 2 cut(s) 754, 1241
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 263, 504
SduI GDGCHC 4 cut(s) 329, 408, 763, 1013
SfaNI GCATC 2 cut(s) 94, 469
SfcI CTRYAG 1 cut(s) 579
SmiMI CAYNNNNRTG 3 cut(s) 465, 504, 1172
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 7 cut(s) 185, 246, 452, 492, 639, 828, 1196
SsiI CCGC 2 cut(s) 75, 322
SspMI CTAG 3 cut(s) 42, 756, 1315
StyD4I CCNGG 2 cut(s) 261, 502
StyI CCWWGG 3 cut(s) 218, 306, 375
TaaI ACNGT 8 cut(s) 337, 443, 467, 812, 944, 967, 996, 1208
TaiI ACGT 2 cut(s) 690, 1306
TaqI TCGA 4 cut(s) 390, 730, 795, 1218
TasI AATT 7 cut(s) 185, 246, 452, 492, 639, 828, 1196
TatI WGTACW 3 cut(s) 752, 812, 1239
TfiI GAWTC 4 cut(s) 647, 778, 980, 1311
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 5 cut(s) 448, 677, 713, 864, 1018
TseFI GTSAC 2 cut(s) 443, 713
TseI GCWGC 9 cut(s) 661, 676, 766, 769, 772, 950, 1108, 1111, 1114
Tsp45I GTSAC 2 cut(s) 443, 713
TspDTI ATGAA 6 cut(s) 554, 680, 924, 948, 993, 1080
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 5 cut(s) 448, 677, 713, 864, 1018
XapI RAATTY 1 cut(s) 452
XceI RCATGY 1 cut(s) 857
XmiI GTMKAC 1 cut(s) 918
XspI CTAG 3 cut(s) 42, 756, 1315
ZrmI AGTACT 2 cut(s) 754, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.