RLG00000009477

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013683
Physical Location & Seq
Forward (+)
53392953 .. 53397873
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009477

Sequence Viewer

Length: 1266 bp
ATGGAGAAAGGTTCGGGCTCTGGTGGCGTCGAGGCAGGGGCTAACAATGTTGGTAAGGGAGGTCCTGCATCCCTGCAATCTCTTGGACCATTTGGTTCCAAGATACTGGGAAACCCCATTGTTTTGGATAACGTTTTGACTGAGATCAAGGCTGTGGGATCTATGCAGCTGTTCGGTTTCAGGGGTGAGGGTCATGAGGCATCGTTGGCTTTGGTTCCGGCTTATGTAGTGACTCGGACAGAGGCTGGCCATGTGAAGTTTTCCTCTCTGCAAATGGGGCCAATGGGGAGGAAAAGAAAGGTCATTACCAAGTTGGATCGTTTTGGGAAAAAATACTTGGCTTTCCCTGATTCTACACTTAAGGTTGAAGATAATGTATCACTAATTCTGCGACACAAGAATGGTAGGATGTTGGTTTCACCAAAGAAGAAGAAGGCCGGGCGTCCTAAGGGGAGCAAGAATAGAGTGAAATCTATTGAGGAACATGAAGAGAAAATTATACGTAAGTATAAACATCGGGGAAAAGCTTTGAGTTTTGATCAAAACCTTGAAGAGTCTGTCCCTGACAAGGTTGGTCCACAGGTGGTTGTGATGGAGGGGGTGCCTGGGACTGCTCAAGCGTTGGAGTCTACTGATGTGCAACAGCAAGTTGATGAGGAATGGATCAATGTTGATGACTTGTTCTGTGGTGACAATGTTGATGAGGAACAAGGTAGTGGTGGTGCCCAGCAGCAGCAGATTCCTTCTCCTCATTTTGACAACAATTACAATTGTACTACTGATGATGTAATTGTATCTGTAAATGAAAGTGAAGATGCAGAGTTAGATGATACTAGTTGGCTTGATGTTGCATTGGAGGAAGTAGACCCTAACCCCTCAGAACAACACAGTTCTGCTGGTCATGATCTAATAATGGCTGTAAATGAGTCGACTTATTTACAGTTTGAGGAGGGCGCTGATCAGAATGGCTCTGCATCGGAGGGAGTAGGAGGGGAGACACTACCCCCTGCCCTCTCAGAAAAACATACATCTTCATCACCATTGACGTTGCAGCAGCAGCACCTGTTCTCTCAAGATGATGATCAAACATTTGATTTTGCTTGTGATAGTGATATCATTCCTATGGATGGTGCCGCTTACTTTCAGTCTGATGAATTTAGCAACTTTTACTTCCACTTCGAAAATCACTACTTATCTGGAGAAGCTACACTACCACAGTTAGTACCTCATCTTGGAGCCGACAGTGGGTTACTTTTTGGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

45.81

Weight (kDa)

4.74

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 601, 722, 1130
AccI GTMKAC 3 cut(s) 629, 864, 929
AciI CCGC 1 cut(s) 1134
AclI AACGTT 1 cut(s) 132
AclWI GGATC 3 cut(s) 166, 324, 671
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 1 cut(s) 1154
AcyI GRCGYC 2 cut(s) 27, 442
AfaI GTAC 2 cut(s) 775, 1224
AfiI CCNNNNNNNGG 4 cut(s) 568, 1127, 1232, 1245
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 368, 551
AhlI ACTAGT 1 cut(s) 833
AjnI CCWGG 1 cut(s) 604
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 3 cut(s) 169, 527, 1205
AluI AGCT 3 cut(s) 169, 527, 1205
Alw26I GTCTC 1 cut(s) 989
AlwI GGATC 3 cut(s) 166, 324, 671
AlwNI CAGNNNCTG 2 cut(s) 245, 1063
AoxI GGCC 3 cut(s) 247, 278, 435
ApeKI GCWGC 6 cut(s) 166, 730, 733, 1051, 1054, 1057
ApoI RAATTY 1 cut(s) 1154
AspLEI GCGC 1 cut(s) 956
AspS9I GGNCC 4 cut(s) 62, 86, 278, 575
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 4 cut(s) 197, 411, 701, 1029
AsuII TTCGAA 1 cut(s) 1179
AvaII GGWCC 3 cut(s) 62, 86, 575
AxyI CCTNAGG 1 cut(s) 447
BaeGI GKGCMC 1 cut(s) 727
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 3 cut(s) 601, 722, 1130
BanII GRGCYC 1 cut(s) 20
BarI GAAGNNNNNNTAC 2 cut(s) 1194, 1226
BbvI GCAGC 6 cut(s) 178, 742, 745, 1063, 1066, 1069
BccI CCATC 2 cut(s) 586, 1121
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 3 cut(s) 538, 958, 1081
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 989
BcuI ACTAGT 1 cut(s) 833
BfaI CTAG 1 cut(s) 834
BfoI RGCGCY 1 cut(s) 957
BfrI CTTAAG 1 cut(s) 359
BisI GCNGC 7 cut(s) 167, 731, 734, 1052, 1055, 1058, 1134
BlsI GCNGC 7 cut(s) 168, 732, 735, 1053, 1056, 1059, 1135
Bme1390I CCNGG 2 cut(s) 439, 606
Bme18I GGWCC 3 cut(s) 62, 86, 575
BmgT120I GGNCC 4 cut(s) 62, 86, 278, 575
BmiI GGNNCC 7 cut(s) 97, 216, 279, 603, 724, 1132, 1237
BmrFI CCNGG 2 cut(s) 439, 606
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 4 cut(s) 77, 209, 805, 983
BmuI ACTGGG 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 1218
Bpu14I TTCGAA 1 cut(s) 1179
BpuEI CTTGAG 2 cut(s) 600, 1056
BpuMI CCSGG 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 1080
BsaHI GRCGYC 2 cut(s) 27, 442
BsaJI CCNNGG 1 cut(s) 605
Bsc4I CCNNNNNNNGG 4 cut(s) 568, 1127, 1232, 1245
Bse1I ACTGG 1 cut(s) 111
Bse21I CCTNAGG 1 cut(s) 447
Bse8I GATNNNNATC 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 605
BseGI GGATG 3 cut(s) 68, 414, 1132
BseJI GATNNNNATC 1 cut(s) 1080
BseLI CCNNNNNNNGG 4 cut(s) 568, 1127, 1232, 1245
BseMII CTCAG 3 cut(s) 132, 891, 1029
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 2 cut(s) 738, 962
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 6 cut(s) 178, 742, 745, 1063, 1066, 1069
BseYI CCCAGC 1 cut(s) 726
BshFI GGCC 3 cut(s) 249, 280, 437
BshNI GGYRCC 3 cut(s) 601, 722, 1130
BsiSI CCGG 2 cut(s) 218, 438
BslFI GGGAC 2 cut(s) 545, 622
BslI CCNNNNNNNGG 4 cut(s) 568, 1127, 1232, 1245
BsmAI GTCTC 1 cut(s) 989
BsmFI GGGAC 2 cut(s) 545, 622
BsnI GGCC 3 cut(s) 249, 280, 437
Bsp119I TTCGAA 1 cut(s) 1179
Bsp1286I GDGCHC 2 cut(s) 20, 727
Bsp143I GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
BspACI CCGC 1 cut(s) 1134
BspANI GGCC 3 cut(s) 249, 280, 437
BspCNI CTCAG 3 cut(s) 133, 890, 1028
BspHI TCATGA 2 cut(s) 193, 901
BspLI GGNNCC 7 cut(s) 97, 216, 279, 603, 724, 1132, 1237
BspPI GGATC 3 cut(s) 166, 324, 671
BspT104I TTCGAA 1 cut(s) 1179
BspT107I GGYRCC 3 cut(s) 601, 722, 1130
BspTI CTTAAG 1 cut(s) 359
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 605
BssMI GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
BssNI GRCGYC 2 cut(s) 27, 442
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 4 cut(s) 890, 942, 1218, 1244
Bst6I CTCTTC 2 cut(s) 483, 546
BstACI GRCGYC 2 cut(s) 27, 442
BstAFI CTTAAG 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 503
BstBI TTCGAA 1 cut(s) 1179
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 4 cut(s) 141, 447, 877, 1015
BstF5I GGATG 3 cut(s) 68, 414, 1132
BstH2I RGCGCY 1 cut(s) 957
BstHHI GCGC 1 cut(s) 956
BstKTI GATC 8 cut(s) 147, 161, 319, 541, 666, 907, 961, 1084
BstMAI GTCTC 1 cut(s) 989
BstMBI GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
BstMWI GCNNNNNNNGC 4 cut(s) 24, 206, 277, 1057
BstNI CCWGG 1 cut(s) 606
BstSCI CCNGG 2 cut(s) 437, 604
BstSLI GKGCMC 1 cut(s) 727
BstSNI TACGTA 1 cut(s) 503
BstV1I GCAGC 6 cut(s) 178, 742, 745, 1063, 1066, 1069
BstX2I RGATCY 1 cut(s) 158
BstXI CCANNNNNNTGG 2 cut(s) 106, 124
BstYI RGATCY 1 cut(s) 158
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 3 cut(s) 249, 280, 437
BtsCI GGATG 3 cut(s) 68, 414, 1132
BtsIMutI CAGTG 1 cut(s) 1249
Cac8I GCNNGC 1 cut(s) 247
CaiI CAGNNNCTG 2 cut(s) 245, 1063
CciI TCATGA 2 cut(s) 193, 901
CfoI GCGC 1 cut(s) 956
Cfr13I GGNCC 4 cut(s) 62, 86, 278, 575
CseI GACGC 2 cut(s) 16, 431
Csp6I GTAC 2 cut(s) 774, 1223
CviAII CATG 4 cut(s) 194, 251, 485, 902
CviQI GTAC 2 cut(s) 774, 1223
DdeI CTNAG 4 cut(s) 141, 447, 877, 1015
DpnI GATC 8 cut(s) 146, 160, 318, 540, 665, 906, 960, 1083
DpnII GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 483, 546
EarI CTCTTC 2 cut(s) 483, 546
Eco105I TACGTA 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 3 cut(s) 62, 86, 575
Eco81I CCTNAGG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 604
EcoRV GATATC 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 20
FaeI CATG 4 cut(s) 197, 254, 488, 905
FaqI GGGAC 2 cut(s) 545, 622
FatI CATG 4 cut(s) 193, 250, 484, 901
FbaI TGATCA 3 cut(s) 538, 958, 1081
FblI GTMKAC 3 cut(s) 629, 864, 929
Fnu4HI GCNGC 7 cut(s) 167, 731, 734, 1052, 1055, 1058, 1134
FokI GGATG 3 cut(s) 55, 421, 1139
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 7 cut(s) 167, 731, 734, 1052, 1055, 1058, 1134
FspBI CTAG 1 cut(s) 834
GlaI GCGC 1 cut(s) 955
GluI GCNGC 7 cut(s) 167, 731, 734, 1052, 1055, 1058, 1134
GsaI CCCAGC 1 cut(s) 730
GsuI CTGGAG 1 cut(s) 1218
HaeII RGCGCY 1 cut(s) 957
HaeIII GGCC 3 cut(s) 249, 280, 437
HapII CCGG 2 cut(s) 218, 438
HgaI GACGC 2 cut(s) 16, 431
HhaI GCGC 1 cut(s) 956
Hin1I GRCGYC 2 cut(s) 27, 442
Hin1II CATG 4 cut(s) 197, 254, 488, 905
Hin6I GCGC 1 cut(s) 954
HinP1I GCGC 1 cut(s) 954
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 6 cut(s) 232, 350, 554, 626, 739, 926
HpaII CCGG 2 cut(s) 218, 438
HphI GGTGA 4 cut(s) 197, 411, 701, 1029
Hpy166II GTNNAC 4 cut(s) 578, 630, 865, 930
Hpy188I TCNGA 6 cut(s) 237, 880, 963, 979, 1018, 1150
Hpy188III TCNNGA 4 cut(s) 194, 902, 1073, 1197
Hpy8I GTNNAC 4 cut(s) 578, 630, 865, 930
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 427, 753
HpyCH4III ACNGT 4 cut(s) 890, 942, 1218, 1244
HpyCH4IV ACGT 3 cut(s) 132, 502, 1046
HpyCH4V TGCA 9 cut(s) 68, 76, 166, 271, 640, 818, 851, 974, 1051
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 206, 277, 1057
HpyF3I CTNAG 4 cut(s) 141, 447, 877, 1015
HpySE526I ACGT 3 cut(s) 132, 502, 1046
Hsp92I GRCGYC 2 cut(s) 27, 442
Hsp92II CATG 4 cut(s) 197, 254, 488, 905
HspAI GCGC 1 cut(s) 954
Ksp22I TGATCA 3 cut(s) 538, 958, 1081
Kzo9I GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
LmnI GCTCC 2 cut(s) 453, 1235
Lsp1109I GCAGC 6 cut(s) 178, 742, 745, 1063, 1066, 1069
LweI GCATC 4 cut(s) 77, 209, 805, 983
MaeI CTAG 1 cut(s) 834
MaeII ACGT 3 cut(s) 132, 502, 1046
MaeIII GTNAC 3 cut(s) 229, 689, 1248
MalI GATC 8 cut(s) 146, 160, 318, 540, 665, 906, 960, 1083
MboI GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
MboII GAAGA 7 cut(s) 380, 439, 442, 500, 563, 824, 1023
MfeI CAATTG 1 cut(s) 769
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 2 cut(s) 20, 727
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 6 cut(s) 384, 495, 763, 769, 789, 1154
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 4 cut(s) 226, 563, 635, 935
MmeI TCCRAC 2 cut(s) 294, 603
Mox20I TGGCCA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 2 cut(s) 399, 1121
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 169
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 2 cut(s) 218, 438
MspR9I CCNGG 2 cut(s) 439, 606
MunI CAATTG 1 cut(s) 769
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 4 cut(s) 24, 206, 277, 1057
NciI CCSGG 1 cut(s) 439
NdeII GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
NlaIII CATG 4 cut(s) 197, 254, 488, 905
NlaIV GGNNCC 7 cut(s) 97, 216, 279, 603, 724, 1132, 1237
NmuCI GTSAC 2 cut(s) 229, 689
NspV TTCGAA 1 cut(s) 1179
PagI TCATGA 2 cut(s) 193, 901
PfeI GAWTC 2 cut(s) 350, 739
PkrI GCNGC 7 cut(s) 168, 732, 735, 1053, 1056, 1059, 1135
PleI GAGTC 4 cut(s) 226, 562, 634, 934
PpsI GAGTC 4 cut(s) 226, 562, 634, 934
Ppu21I YACGTR 1 cut(s) 503
PpuMI RGGWCCY 1 cut(s) 62
Psp1406I AACGTT 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 604
PspFI CCCAGC 1 cut(s) 726
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 7 cut(s) 97, 216, 279, 603, 724, 1132, 1237
PspPI GGNCC 4 cut(s) 62, 86, 278, 575
PspPPI RGGWCCY 1 cut(s) 62
PstNI CAGNNNCTG 2 cut(s) 245, 1063
PsuI RGATCY 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 169
RsaI GTAC 2 cut(s) 775, 1224
RsaNI GTAC 2 cut(s) 774, 1223
RseI CAYNNNNRTG 2 cut(s) 399, 1121
SalI GTCGAC 1 cut(s) 928
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 7 cut(s) 167, 731, 734, 1052, 1055, 1058, 1134
Sau3AI GATC 8 cut(s) 144, 158, 316, 538, 663, 904, 958, 1081
Sau96I GGNCC 4 cut(s) 62, 86, 278, 575
SchI GAGTC 4 cut(s) 226, 563, 635, 935
ScrFI CCNGG 2 cut(s) 439, 606
SduI GDGCHC 2 cut(s) 20, 727
SfaNI GCATC 4 cut(s) 77, 209, 805, 983
SfuI TTCGAA 1 cut(s) 1179
SinI GGWCC 3 cut(s) 62, 86, 575
SmiMI CAYNNNNRTG 2 cut(s) 399, 1121
SmlI CTYRAG 3 cut(s) 359, 615, 1071
SmoI CTYRAG 3 cut(s) 359, 615, 1071
SnaBI TACGTA 1 cut(s) 503
SpeI ACTAGT 1 cut(s) 833
Sse9I AATT 6 cut(s) 384, 495, 763, 769, 789, 1154
SsiI CCGC 1 cut(s) 1134
SspMI CTAG 1 cut(s) 834
StyD4I CCNGG 2 cut(s) 437, 604
TaaI ACNGT 4 cut(s) 890, 942, 1218, 1244
TaiI ACGT 3 cut(s) 135, 505, 1049
TaqI TCGA 3 cut(s) 30, 929, 1179
TasI AATT 6 cut(s) 384, 495, 763, 769, 789, 1154
TatI WGTACW 1 cut(s) 773
TauI GCSGC 1 cut(s) 1136
TfiI GAWTC 2 cut(s) 350, 739
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 1249
TseFI GTSAC 2 cut(s) 229, 689
TseI GCWGC 6 cut(s) 166, 730, 733, 1051, 1054, 1057
Tsp45I GTSAC 2 cut(s) 229, 689
TspDTI ATGAA 4 cut(s) 501, 819, 1023, 1167
TspRI CASTG 1 cut(s) 1249
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 3 cut(s) 62, 86, 575
XapI RAATTY 1 cut(s) 1154
XmiI GTMKAC 3 cut(s) 629, 864, 929
XspI CTAG 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.