RLG00000009463

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013683
Physical Location & Seq
Forward (+)
53266510 .. 53270650
4141 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009463

Sequence Viewer

Length: 570 bp
ATGGAAATTGAGAAGGACTTACAGTCTGAACAGGATGATAATACCCAAAACCTGCTAGCTGCTGTTGCTTCTGAAGATCCTGCACCGGTTGAAGTAAGCACTCAAGAAAATAGGGGAGACAAGCGCCAACTACCTCCCTCTCCTGCAAAGTCTCCTCAAAAGCTCAAGATCCTACTGCCAGAGCTCTTTAAATCACTCCTCCCTGATGTTCAGGCAAAGAAACAAAAAATGCCAATGTTCACAAGCTATGGGATAATGGGAAACCCCATTGTTTTGGATAACGTTTTGACTGAGATCAAGGTTATGGGATCTATGCAGCTGTTCAGTTTCAGGGGTGAGGGTCATGAGGCATCGTTGGCTTTGGTTCCGCCTTATGTAGTGACTCAGACAAAGGCTAGCCATGTGAAGCTTTCCTCTCTGCAAATGGGGCCAATGGGGAGGAAAAGAAAGGTTGGTCCACAGGTGGTTGTGATGGAGGGGGAGCCTGAGACTGCTCAAACGTTGGAGTCTACTGATGTGGTGGGCTCGGTGGCATGTGATGGCTCACATTCTGCCAATTTTGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

20.51

Weight (kDa)

5.43

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 509
AciI CCGC 1 cut(s) 368
AclI AACGTT 2 cut(s) 282, 500
AclWI GGATC 3 cut(s) 71, 163, 316
AcuI CTGAAG 1 cut(s) 93
AgeI ACCGGT 1 cut(s) 85
AgsI TTSAA 1 cut(s) 92
AhdI GACNNNNNGTC 1 cut(s) 22
AluBI AGCT 6 cut(s) 59, 163, 184, 246, 319, 409
AluI AGCT 6 cut(s) 59, 163, 184, 246, 319, 409
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 3 cut(s) 111, 156, 482
AlwI GGATC 3 cut(s) 71, 163, 316
AoxI GGCC 1 cut(s) 428
ApeKI GCWGC 2 cut(s) 59, 316
AsiGI ACCGGT 1 cut(s) 85
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 2 cut(s) 428, 455
AsuHPI GGTGA 1 cut(s) 347
AsuNHI GCTAGC 2 cut(s) 55, 395
AvaII GGWCC 1 cut(s) 455
BanII GRGCYC 2 cut(s) 186, 527
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 46, 328
BccI CCATC 2 cut(s) 466, 533
BcoDI GTCTC 3 cut(s) 111, 156, 482
BfaI CTAG 2 cut(s) 56, 396
BfoI RGCGCY 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 60
BisI GCNGC 2 cut(s) 60, 317
BlsI GCNGC 2 cut(s) 61, 318
Bme18I GGWCC 1 cut(s) 455
BmeRI GACNNNNNGTC 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 428, 455
BmiI GGNNCC 3 cut(s) 366, 429, 483
BmsI GCATC 1 cut(s) 359
BmtI GCTAGC 2 cut(s) 59, 399
BpuEI CTTGAG 2 cut(s) 87, 149
BsaWI WCCGGW 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 85
BseGI GGATG 1 cut(s) 40
BseMII CTCAG 3 cut(s) 282, 398, 477
BseRI GAGGAG 2 cut(s) 144, 188
BseXI GCAGC 2 cut(s) 46, 328
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 1 cut(s) 430
BshTI ACCGGT 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 86
BsmAI GTCTC 3 cut(s) 111, 156, 482
BsnI GGCC 1 cut(s) 430
Bsp1286I GDGCHC 2 cut(s) 186, 527
Bsp143I GATC 4 cut(s) 76, 168, 294, 308
BspACI CCGC 1 cut(s) 368
BspANI GGCC 1 cut(s) 430
BspCNI CTCAG 3 cut(s) 283, 397, 478
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 3 cut(s) 366, 429, 483
BspMI ACCTGC 1 cut(s) 60
BspOI GCTAGC 2 cut(s) 59, 399
BspPI GGATC 3 cut(s) 71, 163, 316
BsrFI RCCGGY 1 cut(s) 85
BssAI RCCGGY 1 cut(s) 85
BssMI GATC 4 cut(s) 76, 168, 294, 308
Bst4CI ACNGT 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 57, 397
BstDEI CTNAG 3 cut(s) 291, 384, 486
BstF5I GGATG 1 cut(s) 40
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 4 cut(s) 79, 171, 297, 311
BstMAI GTCTC 3 cut(s) 111, 156, 482
BstMBI GATC 4 cut(s) 76, 168, 294, 308
BstMWI GCNNNNNNNGC 3 cut(s) 65, 356, 427
BstNSI RCATGY 1 cut(s) 537
BstV1I GCAGC 2 cut(s) 46, 328
BstX2I RGATCY 3 cut(s) 76, 168, 308
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 3 cut(s) 76, 168, 308
BsuRI GGCC 1 cut(s) 430
BtsCI GGATG 1 cut(s) 40
BveI ACCTGC 1 cut(s) 60
Cac8I GCNNGC 2 cut(s) 57, 397
CciI TCATGA 1 cut(s) 343
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 2 cut(s) 428, 455
CspAI ACCGGT 1 cut(s) 85
CviAII CATG 3 cut(s) 344, 401, 534
DdeI CTNAG 3 cut(s) 291, 384, 486
DpnI GATC 4 cut(s) 78, 170, 296, 310
DpnII GATC 4 cut(s) 76, 168, 294, 308
DraI TTTAAA 1 cut(s) 190
DriI GACNNNNNGTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 22
EciI GGCGGA 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 2 cut(s) 186, 527
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 184
EcoT38I GRGCYC 2 cut(s) 186, 527
FaeI CATG 3 cut(s) 347, 404, 537
FaiI YATR 7 cut(s) 249, 305, 314, 345, 375, 402, 535
FatI CATG 3 cut(s) 343, 400, 533
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 2 cut(s) 60, 317
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 2 cut(s) 186, 527
Fsp4HI GCNGC 2 cut(s) 60, 317
FspBI CTAG 2 cut(s) 56, 396
GlaI GCGC 1 cut(s) 125
GluI GCNGC 2 cut(s) 60, 317
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 1 cut(s) 430
HapII CCGG 1 cut(s) 86
HhaI GCGC 1 cut(s) 126
Hin1II CATG 3 cut(s) 347, 404, 537
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 2 cut(s) 382, 506
HpaII CCGG 1 cut(s) 86
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 3 cut(s) 240, 458, 510
Hpy188I TCNGA 3 cut(s) 28, 73, 387
Hpy188III TCNNGA 3 cut(s) 104, 166, 344
Hpy8I GTNNAC 3 cut(s) 240, 458, 510
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4IV ACGT 2 cut(s) 282, 500
HpyCH4V TGCA 4 cut(s) 83, 146, 316, 421
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 356, 427
HpyF3I CTNAG 3 cut(s) 291, 384, 486
HpySE526I ACGT 2 cut(s) 282, 500
Hsp92II CATG 3 cut(s) 347, 404, 537
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 4 cut(s) 76, 168, 294, 308
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 2 cut(s) 46, 328
LweI GCATC 1 cut(s) 359
MaeI CTAG 2 cut(s) 56, 396
MaeII ACGT 2 cut(s) 282, 500
MaeIII GTNAC 1 cut(s) 379
MalI GATC 4 cut(s) 78, 170, 296, 310
MboI GATC 4 cut(s) 76, 168, 294, 308
MboII GAAGA 1 cut(s) 86
MflI RGATCY 3 cut(s) 76, 168, 308
MhlI GDGCHC 2 cut(s) 186, 527
MluCI AATT 2 cut(s) 6, 556
MlyI GAGTC 2 cut(s) 376, 515
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 9 cut(s) 144, 148, 165, 209, 331, 340, 424, 432, 469
MseI TTAA 2 cut(s) 189, 568
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 1 cut(s) 86
MwoI GCNNNNNNNGC 3 cut(s) 65, 356, 427
NdeII GATC 4 cut(s) 76, 168, 294, 308
NheI GCTAGC 2 cut(s) 55, 395
NlaIII CATG 3 cut(s) 347, 404, 537
NlaIV GGNNCC 3 cut(s) 366, 429, 483
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 537
PagI TCATGA 1 cut(s) 343
PinAI ACCGGT 1 cut(s) 85
PkrI GCNGC 2 cut(s) 61, 318
PleI GAGTC 2 cut(s) 376, 514
PpsI GAGTC 2 cut(s) 376, 514
Psp124BI GAGCTC 1 cut(s) 186
Psp1406I AACGTT 2 cut(s) 282, 500
PspN4I GGNNCC 3 cut(s) 366, 429, 483
PspPI GGNCC 2 cut(s) 428, 455
PsuI RGATCY 3 cut(s) 76, 168, 308
PvuII CAGCTG 1 cut(s) 319
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 189, 568
SatI GCNGC 2 cut(s) 60, 317
Sau3AI GATC 4 cut(s) 76, 168, 294, 308
Sau96I GGNCC 2 cut(s) 428, 455
SchI GAGTC 2 cut(s) 376, 515
SduI GDGCHC 2 cut(s) 186, 527
SfaNI GCATC 1 cut(s) 359
SinI GGWCC 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 102, 164
SmoI CTYRAG 2 cut(s) 102, 164
Sse9I AATT 2 cut(s) 6, 556
SsiI CCGC 1 cut(s) 368
SspMI CTAG 2 cut(s) 56, 396
SstI GAGCTC 1 cut(s) 186
TaaI ACNGT 1 cut(s) 24
TaiI ACGT 2 cut(s) 285, 503
TasI AATT 2 cut(s) 6, 556
Tru1I TTAA 2 cut(s) 189, 568
Tru9I TTAA 2 cut(s) 189, 568
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 59, 316
Tsp45I GTSAC 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 455
XceI RCATGY 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 509
XspI CTAG 2 cut(s) 56, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.