FvH4_5g39080

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005917
Physical Location & Seq
Forward (+)
28843887 .. 28846385
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39080.t1

Sequence Viewer

Length: 1950 bp
ATGGCAAATGCATTCTTCTCGTCCACACCACTTGATTTCGGAATCCCAGGCGGTTTGAGTCCCCCCCAGCTTGAAGAATATGACAAAGAAGATACAGTAATGTTCAATGTTGGCAAGGGAAACCATTTGTTTGGTTTAGATGATGATCAGTTGGGAGAAATGAATACTCTTTCTTCTGAACATGGTTTCTACCAAGACAACAAGGCCAAGAAAGGAGGAGTTGTATCCTATTTGTCAAAAGATCAAAACCAACACCATGATCTTGACCACCAACAGCAGCAACAGCAATCCAAGCCAAACTTTTTGATTTTGGATAACTTCAACTTCGAGACCGTTTCTTCTCAACATGGTTTCTATCATGGCAGCATGGCCGGAATGAATGACCAACAACAACAGCAACAGGTCAAGTTGAGTTCTTCATGTTTGGAGAATTACAGCAGCTTTAATTTTAGCTTCCAGTCAGGACAAGTTACACAACCAGTCAAAGATGGTGCTCAGTTTAGGCAGAACCATTGCTCAGATATTGTTGAAGCTAACAAGCAATTACCGCCTCCTCAATCTTCTATAGCAACACTGGAGCTCCTAAAAAGTTATGGAAGCGCGTTCAAGATGTTAAAGAGGGAAAGACAAAGTAGTAGCAGGACAACTAGAGGCAATGAAATTGATCAAAACAACAACTATGATGTTAATGGTACACAAAAACTGTCAACCGAAGAGGTCATGAGGGTTGCAGGAGCAAGGTATGTGCAATTCTCTAGTCAGGGGTATGAAGACTTTTACATGCCTATGCACCATTTTGGCTATGCGCTTTCAGGTCTTTGCGAAGAAGAAACGAAAGATGTAGAAATTGCTCACTTACTGTTAGCTGCAGCAGAGAAAGTAGGCTACCAACAATTTGAAAGAGCAAGCAGATTGCTTCTGCAGTGTGAAGGGATTGCATCCTTCAAAGGCACTCTTGTTCAAAAAGTTGTGTGCTACTTCGCTGAGGCGCTTCGTGAGAGGATTGACATGGAAACAGGAATGTATTCATCTAAGGGAAATCAAGAAATGTCTCATTATGTACAGGGTTTGGGATCAAACCTTGCATACATCGCATGCCACCAAGAAGTCCCTTTCCATCCAGTACTGCAGTTCGCGGCAATCCAAGCCATGATAGAAGCTGTTGCAAATGAAAGTAGGATCCATGTGATTGATCTTGAAATCAGGTTTGGTGTGCAATGGACAGGGTTTATTAAAGCACTGGCAGAACGAAAAAAATGTCCGGTTGAGCTTCTAAGTATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGAAGAGGCTGGCAAGTTTGGCAAAGTCCTTGAACCTACCCTTCAAATTTAAGGAAGTTATTGTATCAGACATGAAAGACATCAATGGCCATTTTTTCAATGAAATCAAAGATGATGAAGCTCTTGTTGTGTATGCGTCATTTGTACTGAGGACAATGATACCAAGGCCCAGGTGCTTGGAGAATTTGATGAGGGTGATGAGAAATATCAACCCTTCTTTGATGGTGGTCATTGAAGTGGAAGCTAACCATAACTCACCCTCATTTGTGAACCGTTTCATTGATGCATTGTTTTACTATAGTGCATTTTTTGACTGCCTTGAAAATTGTATGAAACATAACAAGTACAGGGTTATCATGGAAGACTTGTTACGCAAGGGAATCCGGAACATAGTGGCTGCAGAAGGGAGGGAAAGGGTTGTTCGGAGTGTGAAGCTGGATGTGTGGAGGGCCTTTTTTGCTAAGTTTGGAATGGTGGAAACGAGCTTTAGTAACTCATCATTGTATCAAGCTAGTCTGGTGGCTAAAAAGGTGTGTTGCACAACACTTGATAGGAATGGGAAAGGTCTCACTGTTGGGTGGAAAGGAACCCCAATCCATTCGCTTTCAGCTTGGGAATTCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

650

Amino Acids

73.41

Weight (kDa)

6.69

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 284 - 647 1.5e-79 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 602, 1136
AccIII TCCGGA 1 cut(s) 1707
AciI CCGC 3 cut(s) 51, 548, 1136
AclWI GGATC 3 cut(s) 1081, 1174, 1187
AcoI YGGCCR 2 cut(s) 369, 1411
AcsI RAATTY 3 cut(s) 1370, 1507, 1940
AfaI GTAC 5 cut(s) 694, 1062, 1125, 1470, 1670
AjnI CCWGG 2 cut(s) 46, 1493
AjuI GAANNNNNNNTTGG 2 cut(s) 1191, 1223
Alw21I GWGCWC 2 cut(s) 496, 582
Alw26I GTCTC 4 cut(s) 323, 1056, 1313, 1895
AlwI GGATC 3 cut(s) 1081, 1174, 1187
Aor13HI TCCGGA 1 cut(s) 1707
AoxI GGCC 5 cut(s) 204, 369, 1411, 1490, 1773
ApeKI GCWGC 6 cut(s) 277, 363, 438, 866, 869, 1721
ApoI RAATTY 3 cut(s) 1370, 1507, 1940
ArsI GACNNNNNNTTYG 2 cut(s) 1243, 1275
Asp700I GAANNNNTTC 2 cut(s) 1024, 1598
AspLEI GCGC 3 cut(s) 602, 808, 991
AspS9I GGNCC 2 cut(s) 1491, 1773
AsuHPI GGTGA 2 cut(s) 1531, 1572
BalI TGGCCA 1 cut(s) 1413
BamHI GGATCC 1 cut(s) 1179
BanII GRGCYC 1 cut(s) 582
BarI GAAGNNNNNNTAC 2 cut(s) 1677, 1709
BbsI GAAGAC 2 cut(s) 777, 1692
Bbv12I GWGCWC 2 cut(s) 496, 582
BbvCI CCTCAGC 1 cut(s) 984
BbvI GCAGC 6 cut(s) 289, 375, 450, 853, 881, 1708
BccI CCATC 3 cut(s) 482, 1125, 1540
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 48, 1495
BciVI GTATCC 1 cut(s) 235
BclI TGATCA 2 cut(s) 145, 664
BcoDI GTCTC 4 cut(s) 323, 1056, 1313, 1895
BfaI CTAG 3 cut(s) 648, 756, 1836
BfmI CTRYAG 6 cut(s) 564, 867, 920, 1127, 1621, 1722
BfoI RGCGCY 1 cut(s) 992
BfuI GTATCC 1 cut(s) 235
BisI GCNGC 7 cut(s) 278, 364, 439, 867, 870, 1137, 1722
BlsI GCNGC 7 cut(s) 279, 365, 440, 868, 871, 1138, 1723
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 2 cut(s) 48, 1495
BmgT120I GGNCC 2 cut(s) 1491, 1773
BmiI GGNNCC 2 cut(s) 1181, 1912
BmrFI CCNGG 2 cut(s) 48, 1495
BmsI GCATC 2 cut(s) 947, 1597
BpiI GAAGAC 2 cut(s) 777, 1692
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 984
BsaBI GATNNNNATC 1 cut(s) 144
BsaI GGTCTC 2 cut(s) 323, 1895
BsaJI CCNNGG 3 cut(s) 46, 1487, 1493
BsaWI WCCGGW 2 cut(s) 1261, 1707
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 564, 594
Bse1I ACTGG 5 cut(s) 457, 479, 579, 1121, 1245
Bse3DI GCAATG 3 cut(s) 511, 661, 1223
Bse8I GATNNNNATC 1 cut(s) 144
BseAI TCCGGA 1 cut(s) 1707
BseBI CCWGG 2 cut(s) 48, 1495
BseDI CCNNGG 3 cut(s) 46, 1487, 1493
BseGI GGATG 3 cut(s) 938, 1117, 1768
BseJI GATNNNNATC 1 cut(s) 144
BseMI GCAATG 3 cut(s) 511, 661, 1223
BseMII CTCAG 4 cut(s) 509, 531, 975, 1463
BseNI ACTGG 5 cut(s) 457, 479, 579, 1121, 1245
BseRI GAGGAG 2 cut(s) 231, 543
BseXI GCAGC 6 cut(s) 289, 375, 450, 853, 881, 1708
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 602, 1136
BshFI GGCC 5 cut(s) 206, 371, 1413, 1492, 1775
BsiHKAI GWGCWC 2 cut(s) 496, 582
BsiSI CCGG 3 cut(s) 372, 1262, 1708
BslFI GGGAC 2 cut(s) 45, 1094
BsmAI GTCTC 4 cut(s) 323, 1056, 1313, 1895
BsmFI GGGAC 2 cut(s) 45, 1094
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 5 cut(s) 206, 371, 1413, 1492, 1775
Bso31I GGTCTC 2 cut(s) 323, 1895
Bsp1286I GDGCHC 2 cut(s) 496, 582
Bsp13I TCCGGA 1 cut(s) 1707
Bsp1407I TGTACA 1 cut(s) 1060
Bsp143I GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
BspACI CCGC 3 cut(s) 51, 548, 1136
BspANI GGCC 5 cut(s) 206, 371, 1413, 1492, 1775
BspCNI CTCAG 4 cut(s) 508, 530, 976, 1464
BspEI TCCGGA 1 cut(s) 1707
BspFNI CGCG 2 cut(s) 602, 1136
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 2 cut(s) 1181, 1912
BspMAI CTGCAG 4 cut(s) 871, 924, 1131, 1726
BspPI GGATC 3 cut(s) 1081, 1174, 1187
BspTNI GGTCTC 2 cut(s) 323, 1895
BsrDI GCAATG 3 cut(s) 511, 661, 1223
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 5 cut(s) 457, 479, 579, 1121, 1245
BssECI CCNNGG 3 cut(s) 46, 1487, 1493
BssMI GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
BssT1I CCWWGG 1 cut(s) 1487
Bst2UI CCWGG 2 cut(s) 48, 1495
Bst4CI ACNGT 6 cut(s) 97, 334, 705, 861, 1598, 1897
Bst6I CTCTTC 2 cut(s) 708, 1322
BstAUI TGTACA 1 cut(s) 1060
BstC8I GCNNGC 3 cut(s) 907, 1096, 1335
BstDEI CTNAG 7 cut(s) 495, 517, 984, 1032, 1274, 1472, 1785
BstF5I GGATG 3 cut(s) 938, 1117, 1768
BstFNI CGCG 2 cut(s) 602, 1136
BstH2I RGCGCY 1 cut(s) 992
BstHHI GCGC 3 cut(s) 602, 808, 991
BstKTI GATC 7 cut(s) 148, 244, 262, 667, 1076, 1182, 1195
BstMAI GTCTC 4 cut(s) 323, 1056, 1313, 1895
BstMBI GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
BstMWI GCNNNNNNNGC 7 cut(s) 283, 292, 547, 1091, 1145, 1343, 1781
BstNI CCWGG 2 cut(s) 48, 1495
BstNSI RCATGY 2 cut(s) 784, 1098
BstSCI CCNGG 2 cut(s) 46, 1493
BstSFI CTRYAG 6 cut(s) 564, 867, 920, 1127, 1621, 1722
BstUI CGCG 2 cut(s) 602, 1136
BstV1I GCAGC 6 cut(s) 289, 375, 450, 853, 881, 1708
BstV2I GAAGAC 2 cut(s) 777, 1692
BstX2I RGATCY 1 cut(s) 1179
BstXI CCANNNNNNTGG 2 cut(s) 131, 1501
BstYI RGATCY 1 cut(s) 1179
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 5 cut(s) 206, 371, 1413, 1492, 1775
BtgZI GCGATG 1 cut(s) 1075
BtsCI GGATG 3 cut(s) 938, 1117, 1768
BtsI GCAGTG 1 cut(s) 929
BtsIMutI CAGTG 4 cut(s) 572, 929, 1238, 1893
Cac8I GCNNGC 3 cut(s) 907, 1096, 1335
CciI TCATGA 1 cut(s) 720
CfoI GCGC 3 cut(s) 602, 808, 991
Cfr13I GGNCC 2 cut(s) 1491, 1773
CseI GACGC 1 cut(s) 1449
Csp6I GTAC 5 cut(s) 693, 1061, 1124, 1469, 1669
CspCI CAANNNNNGTGG 2 cut(s) 13, 48
CviQI GTAC 5 cut(s) 693, 1061, 1124, 1469, 1669
DdeI CTNAG 7 cut(s) 495, 517, 984, 1032, 1274, 1472, 1785
DpnI GATC 7 cut(s) 147, 243, 261, 666, 1075, 1181, 1194
DpnII GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
EaeI YGGCCR 2 cut(s) 369, 1411
Eam1104I CTCTTC 2 cut(s) 708, 1322
EarI CTCTTC 2 cut(s) 708, 1322
Ecl136II GAGCTC 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 1487
Eco24I GRGCYC 1 cut(s) 582
Eco31I GGTCTC 2 cut(s) 323, 1895
Eco53kI GAGCTC 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 580
EcoO109I RGGNCCY 1 cut(s) 1773
EcoRI GAATTC 1 cut(s) 1940
EcoRII CCWGG 2 cut(s) 46, 1493
EcoT14I CCWWGG 1 cut(s) 1487
EcoT22I ATGCAT 2 cut(s) 13, 1612
EcoT38I GRGCYC 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 1487
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 939, 971
FaqI GGGAC 2 cut(s) 45, 1094
FbaI TGATCA 2 cut(s) 145, 664
Fnu4HI GCNGC 7 cut(s) 278, 364, 439, 867, 870, 1137, 1722
FokI GGATG 3 cut(s) 925, 1104, 1775
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 7 cut(s) 278, 364, 439, 867, 870, 1137, 1722
FspBI CTAG 3 cut(s) 648, 756, 1836
GlaI GCGC 3 cut(s) 601, 807, 990
GluI GCNGC 7 cut(s) 278, 364, 439, 867, 870, 1137, 1722
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 992
HaeIII GGCC 5 cut(s) 206, 371, 1413, 1492, 1775
HapII CCGG 3 cut(s) 372, 1262, 1708
HgaI GACGC 1 cut(s) 1449
HhaI GCGC 3 cut(s) 602, 808, 991
Hin6I GCGC 3 cut(s) 600, 806, 989
HinP1I GCGC 3 cut(s) 600, 806, 989
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 3 cut(s) 42, 58, 1704
HpaII CCGG 3 cut(s) 372, 1262, 1708
HphI GGTGA 2 cut(s) 1531, 1572
Hpy166II GTNNAC 4 cut(s) 24, 695, 708, 1594
Hpy188I TCNGA 5 cut(s) 41, 178, 520, 1393, 1749
Hpy188III TCNNGA 9 cut(s) 263, 328, 462, 607, 721, 995, 1043, 1196, 1708
Hpy8I GTNNAC 4 cut(s) 24, 695, 708, 1594
HpyAV CCTTC 5 cut(s) 923, 952, 1375, 1548, 1721
HpyCH4III ACNGT 6 cut(s) 97, 334, 705, 861, 1598, 1897
HpyF10VI GCNNNNNNNGC 7 cut(s) 283, 292, 547, 1091, 1145, 1343, 1781
HpyF3I CTNAG 7 cut(s) 495, 517, 984, 1032, 1274, 1472, 1785
HspAI GCGC 3 cut(s) 600, 806, 989
Kpn2I TCCGGA 1 cut(s) 1707
Ksp22I TGATCA 2 cut(s) 145, 664
Kzo9I GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
LmnI GCTCC 3 cut(s) 577, 585, 734
Lsp1109I GCAGC 6 cut(s) 289, 375, 450, 853, 881, 1708
LweI GCATC 2 cut(s) 947, 1597
MaeI CTAG 3 cut(s) 648, 756, 1836
MaeIII GTNAC 3 cut(s) 469, 1692, 1814
MalI GATC 7 cut(s) 147, 243, 261, 666, 1075, 1181, 1194
MboI GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
MflI RGATCY 1 cut(s) 1179
MhlI GDGCHC 2 cut(s) 496, 582
MlsI TGGCCA 1 cut(s) 1413
MluNI TGGCCA 1 cut(s) 1413
MlyI GAGTC 1 cut(s) 67
Mox20I TGGCCA 1 cut(s) 1413
Mph1103I ATGCAT 2 cut(s) 13, 1612
MroI TCCGGA 1 cut(s) 1707
MroXI GAANNNNTTC 2 cut(s) 1024, 1598
MscI TGGCCA 1 cut(s) 1413
MseI TTAA 5 cut(s) 444, 614, 687, 1233, 1374
MslI CAYNNNNRTG 2 cut(s) 785, 1559
Msp20I TGGCCA 1 cut(s) 1413
MspI CCGG 3 cut(s) 372, 1262, 1708
MspR9I CCNGG 2 cut(s) 48, 1495
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 2 cut(s) 48, 1495
MvnI CGCG 2 cut(s) 602, 1136
MwoI GCNNNNNNNGC 7 cut(s) 283, 292, 547, 1091, 1145, 1343, 1781
NdeII GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
NlaIV GGNNCC 2 cut(s) 1181, 1912
NsiI ATGCAT 2 cut(s) 13, 1612
NspI RCATGY 2 cut(s) 784, 1098
PaeI GCATGC 1 cut(s) 1098
PagI TCATGA 1 cut(s) 720
PctI GAATGC 1 cut(s) 11
PdmI GAANNNNTTC 2 cut(s) 1024, 1598
PfeI GAWTC 2 cut(s) 42, 1704
PkrI GCNGC 7 cut(s) 279, 365, 440, 868, 871, 1138, 1723
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 46, 1493
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 2 cut(s) 46, 1493
PspN4I GGNNCC 2 cut(s) 1181, 1912
PspPI GGNCC 2 cut(s) 1491, 1773
PstI CTGCAG 4 cut(s) 871, 924, 1131, 1726
PsuI RGATCY 1 cut(s) 1179
RsaI GTAC 5 cut(s) 694, 1062, 1125, 1470, 1670
RsaNI GTAC 5 cut(s) 693, 1061, 1124, 1469, 1669
RseI CAYNNNNRTG 2 cut(s) 785, 1559
SacI GAGCTC 1 cut(s) 582
SaqAI TTAA 5 cut(s) 444, 614, 687, 1233, 1374
SatI GCNGC 7 cut(s) 278, 364, 439, 867, 870, 1137, 1722
Sau3AI GATC 7 cut(s) 145, 241, 259, 664, 1073, 1179, 1192
Sau96I GGNCC 2 cut(s) 1491, 1773
ScaI AGTACT 1 cut(s) 1125
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 48, 1495
SduI GDGCHC 2 cut(s) 496, 582
SfaNI GCATC 2 cut(s) 947, 1597
SfcI CTRYAG 6 cut(s) 564, 867, 920, 1127, 1621, 1722
SmiMI CAYNNNNRTG 2 cut(s) 785, 1559
SphI GCATGC 1 cut(s) 1098
SsiI CCGC 3 cut(s) 51, 548, 1136
SspMI CTAG 3 cut(s) 648, 756, 1836
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 2 cut(s) 46, 1493
StyI CCWWGG 1 cut(s) 1487
TaaI ACNGT 6 cut(s) 97, 334, 705, 861, 1598, 1897
TaqI TCGA 1 cut(s) 327
TatI WGTACW 4 cut(s) 1060, 1123, 1468, 1668
TauI GCSGC 1 cut(s) 1139
TfiI GAWTC 2 cut(s) 42, 1704
Tru1I TTAA 5 cut(s) 444, 614, 687, 1233, 1374
Tru9I TTAA 5 cut(s) 444, 614, 687, 1233, 1374
TscAI CASTG 4 cut(s) 579, 929, 1245, 1900
TseI GCWGC 6 cut(s) 277, 363, 438, 866, 869, 1721
TspGWI ACGGA 1 cut(s) 1934
TspRI CASTG 4 cut(s) 579, 929, 1245, 1900
XapI RAATTY 3 cut(s) 1370, 1507, 1940
XceI RCATGY 2 cut(s) 784, 1098
XmnI GAANNNNTTC 2 cut(s) 1024, 1598
XspI CTAG 3 cut(s) 648, 756, 1836
ZrmI AGTACT 1 cut(s) 1125
Zsp2I ATGCAT 2 cut(s) 13, 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.