Rh7CG514300

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005917
Physical Location & Seq
Forward (+)
69381712 .. 69383951
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG514300.1

Sequence Viewer

Length: 2010 bp
ATGGCAAATGCATTATTCTCGTTCACACCATTTGATTTCGATATCCAAGGCGGTTTGAGTCCCCCCCAGCTTGAAGATTATGACAAAGAAGAAACAGTAATGTTCAATGTTGGCAAGGGAGACCATTTGTTTGGACTAGAACATCAGTTGGGAGGAATGAATACTCCTTCTTCTGAACATGGTTTCTACCAAGACAACAACGCCAAGACAGTAGTAGTATGCCATTTGTCCAAAGATCAAAACCAACACCGTGATCTTGAACACCAACAGCACCAACAGCAATCCAAGACAGACTTTTTTATTCTGGATAACTTCAACTTCGAGACTGTTTCTTCTCAACATGGTTTTTATCATGCCAATAAGGCGGGGAAGGATCACCAACACCACCATTACCAACAACAGCAACAACAATCCAAGTCAAATTTTTCAATTTTGGAGAATTTCAATAACTTTGCTCTTTACTTCCCCTCGGGACAGGTTACAGAACCAGTTGAGGATGGTGCTCGGTTTACACACAACCAGACCAGAGGCTCAGACATTTTCGAAACGAACAAGCAATTGCCGCCTCCTGAATCTTCTCTAGGAACACTGGAGCTCCTAAACAGTTACGGAAGCGCCTTCAAGAAGTTGAAGGGAGAAAGACAAAGCAGTAGCAGGGCAGTTAAGGGTAATGAAATTGATCAAAACAGCCGGTACGTTGTTAATGGTAAACAGAAATTGTCCCCCGAAGAGGTCATGAGGGTTGCTGGAGCAAGGTATGTGCAATTCTCTAGTCAGGGGTATGAAAACTTTTACTTGTCTATGCACCCTTTTGGCTATGCGCTTTCAGGTCTCTGCGAAGAAGAAACGAAAGATGTAGAACTTGTTCACTTACTGTTAGCTGCAGCAGAGAAAGTAGGGTACCAACAATTTGAAAGAGCAAGCAGATTGCTTCTGCAGTGTGAATGGATTCCATCCTTCAAAGGCACTCCTGTTCAGAAAGTTGTGTGCTACTTCGCAGAGGCACTTCGTGAGAGGATCGAAAAAGAAACAGGACAGTATGCATCTAAGGGAAATGAAGAATTGTCTCAATATGCACATGGTTTGGGGACAAACCTTGCATACATCGCATGCCACCAAGAAGTCCCTTTCCATCCCGTACTGCAGTTCGCGGCAATCCAAGCCATGGTCGAAAATCTTACAAATGAGAGTAGGATCCATTTGATTGATCTGGAAATCAGGAGTGGTGTGCAATGGACAGGGTGGATGGAAGCAATTGCAGAACGAGAGGAATGTCCGGTTGAGCTTCTTACTATTACTGCTGTGGGAGTTAGAGGCAAACAGAATATAAAGGAGACAGGGGAGAGGTTGGCGAGTGTGGCAAAGTTCTTGAACCTACCCTTTAAATTTAAGGAAGTTATTGTATCAGACATGCAAGACATTAATAACCAGTTATTCGAAGAAATTGAAGATGATGAAGCTGTGGTTGTGTACGCGCCATTAATACTGAGGACAATGATAGCAAGGCCTAGGTGCTTGGAGAACTTGATGAGGGTGATGAGAAATATCAACCCTTCTTTGATGGTGGTCATTGAAGTGGAAGCTAACCATAACTCCCCATCATTTGTGAACCGTTTCATTGATACATTGTTTTTTTATAGTGCATTTTTCGACTGCCTTGAAACCTGTATGAAACAGGAGAAGTACAGGGTGATCATGGAAGGCTTGTTGCGTGAGGGAATCCGGAACATAGTGGCGGCGGAAGGGAGTGAAAGGGTTGCTCGAAGTGTGAAGATGGATGTGTGGAGGGCCTTCTTTGCAAGGTTTGGCATGGTGGAAATGAGCTTTAGTAACTCATCATTTTATCAAGCTAGTCTGATGGCTAAAAAGTTCTGTTGCACAACACTTGATAGGAATGGAAAATGTCTCACTGTTGGGTGGAAGGGAACCCCAATTCATTCGCTTTCCGCTTGGAAGTTCCGTGTTGAGATGAAGAGAAAGAGAGATGGTCTTGAAAAAAATGTAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

669

Amino Acids

76.39

Weight (kDa)

5.97

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 288 - 652 4e-78 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 900
AccB1I GGYRCC 1 cut(s) 900
AccB7I CCANNNNNTGG 1 cut(s) 1165
AccII CGCG 2 cut(s) 1151, 1475
AccIII TCCGGA 1 cut(s) 1722
AciI CCGC 7 cut(s) 51, 365, 563, 1151, 1736, 1739, 1947
AclWI GGATC 4 cut(s) 381, 1025, 1189, 1202
AcsI RAATTY 3 cut(s) 421, 439, 1385
AdeI CACNNNGTG 1 cut(s) 1010
AfaI GTAC 5 cut(s) 695, 902, 1140, 1472, 1685
AfiI CCNNNNNNNGG 2 cut(s) 730, 1165
AluBI AGCT 8 cut(s) 70, 595, 881, 1285, 1460, 1583, 1824, 1850
AluI AGCT 8 cut(s) 70, 595, 881, 1285, 1460, 1583, 1824, 1850
Alw21I GWGCWC 2 cut(s) 505, 597
Alw26I GTCTC 6 cut(s) 114, 317, 836, 1071, 1328, 1910
AlwI GGATC 4 cut(s) 381, 1025, 1189, 1202
Ama87I CYCGRG 1 cut(s) 469
Aor13HI TCCGGA 1 cut(s) 1722
AoxI GGCC 2 cut(s) 1505, 1788
ApeKI GCWGC 2 cut(s) 881, 884
ApoI RAATTY 3 cut(s) 421, 439, 1385
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AseI ATTAAT 2 cut(s) 1422, 1481
Asp700I GAANNNNTTC 3 cut(s) 864, 948, 1613
Asp718I GGTACC 1 cut(s) 900
AspA2I CCTAGG 1 cut(s) 1508
AspLEI GCGC 3 cut(s) 617, 823, 1477
AspS9I GGNCC 1 cut(s) 1788
AsuHPI GGTGA 3 cut(s) 368, 1546, 1702
AsuII TTCGAA 2 cut(s) 543, 1437
AvaI CYCGRG 1 cut(s) 469
AvrII CCTAGG 1 cut(s) 1508
BamHI GGATCC 1 cut(s) 1194
BanI GGYRCC 1 cut(s) 900
BanII GRGCYC 1 cut(s) 597
Bbv12I GWGCWC 2 cut(s) 505, 597
BbvI GCAGC 2 cut(s) 868, 896
BccI CCATC 9 cut(s) 491, 961, 1140, 1240, 1555, 1606, 1768, 1852, 1979
BcgI CGANNNNNNTGC 1 cut(s) 34
BclI TGATCA 2 cut(s) 679, 1692
BcoDI GTCTC 6 cut(s) 114, 317, 836, 1071, 1328, 1910
BfaI CTAG 5 cut(s) 137, 581, 771, 1509, 1851
BfmI CTRYAG 3 cut(s) 882, 935, 1142
BfoI RGCGCY 1 cut(s) 618
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 5 cut(s) 563, 882, 885, 1152, 1737
BlnI CCTAGG 1 cut(s) 1508
BlsI GCNGC 5 cut(s) 564, 883, 886, 1153, 1738
BmeT110I CYCGRG 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 1788
BmiI GGNNCC 3 cut(s) 902, 1196, 1927
BmsI GCATC 1 cut(s) 1052
BpmI CTGGAG 2 cut(s) 611, 768
Bpu14I TTCGAA 2 cut(s) 543, 1437
BsaI GGTCTC 2 cut(s) 114, 836
BsaJI CCNNGG 4 cut(s) 46, 468, 1164, 1508
BsaWI WCCGGW 2 cut(s) 1276, 1722
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 1577, 1607
Bsc4I CCNNNNNNNGG 2 cut(s) 730, 1165
Bse118I RCCGGY 1 cut(s) 690
Bse1I ACTGG 3 cut(s) 488, 594, 1429
Bse3DI GCAATG 1 cut(s) 1238
BseAI TCCGGA 1 cut(s) 1722
BseDI CCNNGG 4 cut(s) 46, 468, 1164, 1508
BseGI GGATG 5 cut(s) 502, 953, 1132, 1251, 1783
BseLI CCNNNNNNNGG 2 cut(s) 730, 1165
BseMI GCAATG 1 cut(s) 1238
BseMII CTCAG 2 cut(s) 546, 1478
BseNI ACTGG 3 cut(s) 488, 594, 1429
BseXI GCAGC 2 cut(s) 868, 896
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 1151, 1475
BshFI GGCC 2 cut(s) 1507, 1790
BshNI GGYRCC 1 cut(s) 900
BsiHKAI GWGCWC 2 cut(s) 505, 597
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 3 cut(s) 691, 1277, 1723
BslFI GGGAC 5 cut(s) 45, 486, 706, 1102, 1109
BslI CCNNNNNNNGG 2 cut(s) 730, 1165
BsmAI GTCTC 6 cut(s) 114, 317, 836, 1071, 1328, 1910
BsmFI GGGAC 5 cut(s) 45, 486, 706, 1102, 1109
BsnI GGCC 2 cut(s) 1507, 1790
Bso31I GGTCTC 2 cut(s) 114, 836
BsoBI CYCGRG 1 cut(s) 469
Bsp119I TTCGAA 2 cut(s) 543, 1437
Bsp1286I GDGCHC 2 cut(s) 505, 597
Bsp13I TCCGGA 1 cut(s) 1722
Bsp143I GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
Bsp19I CCATGG 1 cut(s) 1164
BspACI CCGC 7 cut(s) 51, 365, 563, 1151, 1736, 1739, 1947
BspANI GGCC 2 cut(s) 1507, 1790
BspCNI CTCAG 2 cut(s) 545, 1479
BspEI TCCGGA 1 cut(s) 1722
BspFNI CGCG 2 cut(s) 1151, 1475
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 3 cut(s) 902, 1196, 1927
BspMAI CTGCAG 3 cut(s) 886, 939, 1146
BspPI GGATC 4 cut(s) 381, 1025, 1189, 1202
BspT104I TTCGAA 2 cut(s) 543, 1437
BspT107I GGYRCC 1 cut(s) 900
BspTNI GGTCTC 2 cut(s) 114, 836
BsrDI GCAATG 1 cut(s) 1238
BsrFI RCCGGY 1 cut(s) 690
BsrI ACTGG 3 cut(s) 488, 594, 1429
BssAI RCCGGY 1 cut(s) 690
BssECI CCNNGG 4 cut(s) 46, 468, 1164, 1508
BssMI GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
BssT1I CCWWGG 3 cut(s) 46, 1164, 1508
Bst4CI ACNGT 9 cut(s) 97, 211, 251, 328, 605, 876, 1038, 1613, 1912
Bst6I CTCTTC 2 cut(s) 723, 1967
BstBI TTCGAA 2 cut(s) 543, 1437
BstC8I GCNNGC 2 cut(s) 922, 1111
BstDEI CTNAG 3 cut(s) 532, 1047, 1487
BstDSI CCRYGG 1 cut(s) 1164
BstF5I GGATG 5 cut(s) 502, 953, 1132, 1251, 1783
BstFNI CGCG 2 cut(s) 1151, 1475
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 3 cut(s) 617, 823, 1477
BstKTI GATC 8 cut(s) 238, 256, 376, 682, 1020, 1197, 1210, 1695
BstMAI GTCTC 6 cut(s) 114, 317, 836, 1071, 1328, 1910
BstMBI GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
BstMWI GCNNNNNNNGC 7 cut(s) 277, 362, 562, 1106, 1160, 1358, 1796
BstNSI RCATGY 2 cut(s) 1113, 1414
BstSFI CTRYAG 3 cut(s) 882, 935, 1142
BstUI CGCG 2 cut(s) 1151, 1475
BstV1I GCAGC 2 cut(s) 868, 896
BstX2I RGATCY 1 cut(s) 1194
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 1194
BsuRI GGCC 2 cut(s) 1507, 1790
BtgI CCRYGG 1 cut(s) 1164
BtgZI GCGATG 1 cut(s) 1090
BtsCI GGATG 5 cut(s) 502, 953, 1132, 1251, 1783
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 3 cut(s) 587, 944, 1908
Cac8I GCNNGC 2 cut(s) 922, 1111
CciI TCATGA 1 cut(s) 735
CfoI GCGC 3 cut(s) 617, 823, 1477
Cfr10I RCCGGY 1 cut(s) 690
Cfr13I GGNCC 1 cut(s) 1788
Csp6I GTAC 5 cut(s) 694, 901, 1139, 1471, 1684
CviQI GTAC 5 cut(s) 694, 901, 1139, 1471, 1684
DdeI CTNAG 3 cut(s) 532, 1047, 1487
DpnI GATC 8 cut(s) 237, 255, 375, 681, 1019, 1196, 1209, 1694
DpnII GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
DraI TTTAAA 1 cut(s) 1384
DraIII CACNNNGTG 1 cut(s) 1010
Eam1104I CTCTTC 2 cut(s) 723, 1967
EarI CTCTTC 2 cut(s) 723, 1967
EciI GGCGGA 1 cut(s) 1754
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 3 cut(s) 46, 1164, 1508
Eco147I AGGCCT 1 cut(s) 1507
Eco24I GRGCYC 1 cut(s) 597
Eco31I GGTCTC 2 cut(s) 114, 836
Eco32I GATATC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 595
Eco88I CYCGRG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 595
EcoO109I RGGNCCY 1 cut(s) 1788
EcoRV GATATC 1 cut(s) 43
EcoT14I CCWWGG 3 cut(s) 46, 1164, 1508
EcoT22I ATGCAT 2 cut(s) 13, 1045
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 3 cut(s) 46, 1164, 1508
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 5 cut(s) 45, 486, 706, 1102, 1109
FauI CCCGC 1 cut(s) 358
FbaI TGATCA 2 cut(s) 679, 1692
Fnu4HI GCNGC 5 cut(s) 563, 882, 885, 1152, 1737
FokI GGATG 5 cut(s) 509, 940, 1119, 1258, 1790
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 5 cut(s) 563, 882, 885, 1152, 1737
FspBI CTAG 5 cut(s) 137, 581, 771, 1509, 1851
GlaI GCGC 3 cut(s) 616, 822, 1476
GluI GCNGC 5 cut(s) 563, 882, 885, 1152, 1737
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 611, 768
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 2 cut(s) 1507, 1790
HapII CCGG 3 cut(s) 691, 1277, 1723
HhaI GCGC 3 cut(s) 617, 823, 1477
Hin6I GCGC 3 cut(s) 615, 821, 1475
HinP1I GCGC 3 cut(s) 615, 821, 1475
HinfI GANTC 4 cut(s) 58, 572, 949, 1719
HpaII CCGG 3 cut(s) 691, 1277, 1723
HphI GGTGA 3 cut(s) 368, 1546, 1702
Hpy166II GTNNAC 6 cut(s) 24, 510, 710, 868, 1471, 1609
Hpy188I TCNGA 5 cut(s) 175, 535, 978, 1408, 1857
Hpy8I GTNNAC 6 cut(s) 24, 510, 710, 868, 1471, 1609
HpyCH4III ACNGT 9 cut(s) 97, 211, 251, 328, 605, 876, 1038, 1613, 1912
HpyCH4IV ACGT 1 cut(s) 696
HpyF10VI GCNNNNNNNGC 7 cut(s) 277, 362, 562, 1106, 1160, 1358, 1796
HpyF3I CTNAG 3 cut(s) 532, 1047, 1487
HpySE526I ACGT 1 cut(s) 696
HspAI GCGC 3 cut(s) 615, 821, 1475
Kpn2I TCCGGA 1 cut(s) 1722
KpnI GGTACC 1 cut(s) 904
Ksp22I TGATCA 2 cut(s) 679, 1692
Kzo9I GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
LmnI GCTCC 3 cut(s) 592, 600, 749
Lsp1109I GCAGC 2 cut(s) 868, 896
LweI GCATC 1 cut(s) 1052
MaeI CTAG 5 cut(s) 137, 581, 771, 1509, 1851
MaeII ACGT 1 cut(s) 696
MaeIII GTNAC 4 cut(s) 478, 605, 1829, 2002
MalI GATC 8 cut(s) 237, 255, 375, 681, 1019, 1196, 1209, 1694
MboI GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
MfeI CAATTG 2 cut(s) 557, 1254
MflI RGATCY 1 cut(s) 1194
MhlI GDGCHC 2 cut(s) 505, 597
MlyI GAGTC 1 cut(s) 67
Mph1103I ATGCAT 2 cut(s) 13, 1045
MroI TCCGGA 1 cut(s) 1722
MroXI GAANNNNTTC 3 cut(s) 864, 948, 1613
MseI TTAA 6 cut(s) 663, 702, 1383, 1389, 1422, 1481
MslI CAYNNNNRTG 1 cut(s) 1574
MspI CCGG 3 cut(s) 691, 1277, 1723
MunI CAATTG 2 cut(s) 557, 1254
MvnI CGCG 2 cut(s) 1151, 1475
MwoI GCNNNNNNNGC 7 cut(s) 277, 362, 562, 1106, 1160, 1358, 1796
NcoI CCATGG 1 cut(s) 1164
NdeII GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
NlaIV GGNNCC 3 cut(s) 902, 1196, 1927
NsiI ATGCAT 2 cut(s) 13, 1045
NspI RCATGY 2 cut(s) 1113, 1414
NspV TTCGAA 2 cut(s) 543, 1437
PaeI GCATGC 1 cut(s) 1113
PagI TCATGA 1 cut(s) 735
PceI AGGCCT 1 cut(s) 1507
PdmI GAANNNNTTC 3 cut(s) 864, 948, 1613
PfeI GAWTC 3 cut(s) 572, 949, 1719
PflMI CCANNNNNTGG 1 cut(s) 1165
PkrI GCNGC 5 cut(s) 564, 883, 886, 1153, 1738
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PshBI ATTAAT 2 cut(s) 1422, 1481
Psp124BI GAGCTC 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 902, 1196, 1927
PspPI GGNCC 1 cut(s) 1788
PsrI GAACNNNNNNTAC 2 cut(s) 849, 881
PstI CTGCAG 3 cut(s) 886, 939, 1146
PsuI RGATCY 1 cut(s) 1194
RsaI GTAC 5 cut(s) 695, 902, 1140, 1472, 1685
RsaNI GTAC 5 cut(s) 694, 901, 1139, 1471, 1684
RseI CAYNNNNRTG 1 cut(s) 1574
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 6 cut(s) 663, 702, 1383, 1389, 1422, 1481
SatI GCNGC 5 cut(s) 563, 882, 885, 1152, 1737
Sau3AI GATC 8 cut(s) 235, 253, 373, 679, 1017, 1194, 1207, 1692
Sau96I GGNCC 1 cut(s) 1788
SchI GAGTC 1 cut(s) 67
SduI GDGCHC 2 cut(s) 505, 597
SfaNI GCATC 1 cut(s) 1052
SfcI CTRYAG 3 cut(s) 882, 935, 1142
SfuI TTCGAA 2 cut(s) 543, 1437
SmiMI CAYNNNNRTG 1 cut(s) 1574
SphI GCATGC 1 cut(s) 1113
SseBI AGGCCT 1 cut(s) 1507
SsiI CCGC 7 cut(s) 51, 365, 563, 1151, 1736, 1739, 1947
SspMI CTAG 5 cut(s) 137, 581, 771, 1509, 1851
SstI GAGCTC 1 cut(s) 597
StuI AGGCCT 1 cut(s) 1507
StyI CCWWGG 3 cut(s) 46, 1164, 1508
TaaI ACNGT 9 cut(s) 97, 211, 251, 328, 605, 876, 1038, 1613, 1912
TaiI ACGT 1 cut(s) 699
TaqI TCGA 8 cut(s) 39, 321, 543, 1020, 1170, 1437, 1650, 1762
TatI WGTACW 1 cut(s) 1683
TauI GCSGC 3 cut(s) 565, 1154, 1739
TfiI GAWTC 3 cut(s) 572, 949, 1719
Tru1I TTAA 6 cut(s) 663, 702, 1383, 1389, 1422, 1481
Tru9I TTAA 6 cut(s) 663, 702, 1383, 1389, 1422, 1481
TscAI CASTG 3 cut(s) 594, 944, 1915
TseI GCWGC 2 cut(s) 881, 884
TspDTI ATGAA 9 cut(s) 173, 687, 798, 1071, 1470, 1606, 1685, 1925, 1985
TspGWI ACGGA 2 cut(s) 624, 1949
TspRI CASTG 3 cut(s) 594, 944, 1915
Van91I CCANNNNNTGG 1 cut(s) 1165
VspI ATTAAT 2 cut(s) 1422, 1481
XapI RAATTY 3 cut(s) 421, 439, 1385
XceI RCATGY 2 cut(s) 1113, 1414
XmaJI CCTAGG 1 cut(s) 1508
XmnI GAANNNNTTC 3 cut(s) 864, 948, 1613
XspI CTAG 5 cut(s) 137, 581, 771, 1509, 1851
Zsp2I ATGCAT 2 cut(s) 13, 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.