MD03G1293100.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005917
Physical Location & Seq
Forward (+)
36993235 .. 36993747
513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1293100.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTACAGCCAAATCGGAGGATCAGACATTGCGGAAACCAACAAGCAAATGCCCCATCAGTCTTCCTTAGCTGCGTTGGACCTCCTGAACAACTACGGCAGTGCGTTCAAGAAGCTGAGGGGAGACAGATTAAGCAACAGATGCAATGAAGCTGAAGCCTCCTCATATTCGATCCAACAAAAACTGTCAACTGAGGAGATCATGAGGGTTGCCGGAGCAAGGTATGTGCAAATCTCTGATCAAGGATATGGCGATTTTTTCATGCCTTTGCACCCGTTTGGGTACGCGCTTTCAGGTCTATCTGAAGAAGAAACGAAAGATGTGGAGCTTGCTCACATCCTTTTAGCTGCAGCAGAGAAGGTAGGCTACCAACAATTTGAGCGCGCAAACCGCCTGCTTCTTCATTGCGAAGTTGATGCCTCCTTCAAGGTCAACGGTGTTCAGAGGGTTGTTTTCTATTTCACCGAGGCGCTTCGCGAGAGAATAAAACAGGAAACGGGGAACAGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

171

Amino Acids

19.1

Weight (kDa)

5.59

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 111 - 164 5.4e-07 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 287, 384, 477
AciI CCGC 2 cut(s) 32, 391
AclWI GGATC 2 cut(s) 28, 166
AcuI CTGAAG 2 cut(s) 174, 324
AfaI GTAC 2 cut(s) 5, 284
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 2 cut(s) 109, 427
AluBI AGCT 5 cut(s) 71, 115, 152, 328, 347
AluI AGCT 5 cut(s) 71, 115, 152, 328, 347
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 2 cut(s) 28, 166
ApeKI GCWGC 3 cut(s) 71, 347, 350
AspLEI GCGC 4 cut(s) 289, 384, 386, 472
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 454
AvaII GGWCC 1 cut(s) 79
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
BbsI GAAGAC 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 58, 334, 362
BccI CCATC 1 cut(s) 63
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 473
BisI GCNGC 3 cut(s) 72, 348, 351
BlsI GCNGC 3 cut(s) 73, 349, 352
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 79
BmsI GCATC 2 cut(s) 131, 406
BpiI GAAGAC 1 cut(s) 54
Bpu10I CCTNAGC 2 cut(s) 67, 116
BsaJI CCNNGG 1 cut(s) 465
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse3DI GCAATG 3 cut(s) 27, 151, 403
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMI GCAATG 3 cut(s) 27, 151, 403
BseMII CTCAG 2 cut(s) 107, 183
BsePI GCGCGC 1 cut(s) 382
BseRI GAGGAG 2 cut(s) 151, 209
BseXI GCAGC 3 cut(s) 58, 334, 362
Bsh1236I CGCG 3 cut(s) 287, 384, 477
BsiSI CCGG 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 117
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 20, 171, 198, 238
Bsp68I TCGCGA 1 cut(s) 477
BspACI CCGC 2 cut(s) 32, 391
BspCNI CTCAG 2 cut(s) 108, 184
BspFNI CGCG 3 cut(s) 287, 384, 477
BspHI TCATGA 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 2 cut(s) 28, 166
BsrDI GCAATG 3 cut(s) 27, 151, 403
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 1 cut(s) 465
BssHII GCGCGC 1 cut(s) 382
BssMI GATC 4 cut(s) 20, 171, 198, 238
Bst4CI ACNGT 2 cut(s) 186, 437
BstAPI GCANNNNNTGC 1 cut(s) 141
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 330, 384, 395
BstDEI CTNAG 3 cut(s) 67, 116, 192
BstF5I GGATG 1 cut(s) 336
BstFNI CGCG 3 cut(s) 287, 384, 477
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 4 cut(s) 289, 384, 386, 472
BstKTI GATC 4 cut(s) 23, 174, 201, 241
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 4 cut(s) 20, 171, 198, 238
BstMWI GCNNNNNNNGC 2 cut(s) 141, 390
BstNSI RCATGY 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 348
BstUI CGCG 3 cut(s) 287, 384, 477
BstV1I GCAGC 3 cut(s) 58, 334, 362
BstV2I GAAGAC 1 cut(s) 54
BtsCI GGATG 1 cut(s) 336
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 106
BtuMI TCGCGA 1 cut(s) 477
Cac8I GCNNGC 3 cut(s) 330, 384, 395
CciI TCATGA 1 cut(s) 201
CfoI GCGC 4 cut(s) 289, 384, 386, 472
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 4, 283
CviAII CATG 3 cut(s) 202, 262, 508
CviJI RGCY 8 cut(s) 9, 71, 115, 152, 158, 328, 347, 366
CviKI_1 RGCY 8 cut(s) 9, 71, 115, 152, 158, 328, 347, 366
CviQI GTAC 2 cut(s) 4, 283
DdeI CTNAG 3 cut(s) 67, 116, 192
DpnI GATC 4 cut(s) 22, 173, 200, 240
DpnII GATC 4 cut(s) 20, 171, 198, 238
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 174, 324
FaeI CATG 3 cut(s) 205, 265, 511
FaiI YATR 6 cut(s) 166, 203, 225, 249, 263, 509
FatI CATG 3 cut(s) 201, 261, 507
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 3 cut(s) 72, 348, 351
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 3 cut(s) 72, 348, 351
GlaI GCGC 4 cut(s) 288, 383, 385, 471
GluI GCNGC 3 cut(s) 72, 348, 351
HaeII RGCGCY 1 cut(s) 473
HapII CCGG 1 cut(s) 213
HhaI GCGC 4 cut(s) 289, 384, 386, 472
Hin1II CATG 3 cut(s) 205, 265, 511
Hin6I GCGC 4 cut(s) 287, 382, 384, 470
HinP1I GCGC 4 cut(s) 287, 382, 384, 470
HincII GTYRAC 2 cut(s) 189, 433
HindII GTYRAC 2 cut(s) 189, 433
HpaII CCGG 1 cut(s) 213
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 2 cut(s) 189, 433
Hpy188I TCNGA 5 cut(s) 17, 25, 238, 304, 444
Hpy188III TCNNGA 4 cut(s) 85, 109, 202, 476
Hpy8I GTNNAC 2 cut(s) 189, 433
HpyAV CCTTC 2 cut(s) 352, 433
HpyCH4III ACNGT 2 cut(s) 186, 437
HpyCH4V TGCA 4 cut(s) 144, 229, 271, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 390
HpyF3I CTNAG 3 cut(s) 67, 116, 192
Hsp92II CATG 3 cut(s) 205, 265, 511
HspAI GCGC 4 cut(s) 287, 382, 384, 470
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 4 cut(s) 20, 171, 198, 238
LmnI GCTCC 2 cut(s) 215, 325
LpnPI CCDG 5 cut(s) 98, 226, 279, 407, 476
Lsp1109I GCAGC 3 cut(s) 58, 334, 362
LweI GCATC 2 cut(s) 131, 406
MalI GATC 4 cut(s) 22, 173, 200, 240
MboI GATC 4 cut(s) 20, 171, 198, 238
MboII GAAGA 4 cut(s) 54, 317, 320, 392
MluCI AATT 1 cut(s) 374
MmeI TCCRAC 2 cut(s) 57, 199
MseI TTAA 1 cut(s) 131
MspI CCGG 1 cut(s) 213
MvnI CGCG 3 cut(s) 287, 384, 477
MwoI GCNNNNNNNGC 2 cut(s) 141, 390
NdeII GATC 4 cut(s) 20, 171, 198, 238
NlaIII CATG 3 cut(s) 205, 265, 511
NruI TCGCGA 1 cut(s) 477
NspI RCATGY 1 cut(s) 511
PagI TCATGA 1 cut(s) 201
PauI GCGCGC 1 cut(s) 382
PkrI GCNGC 3 cut(s) 73, 349, 352
PspPI GGNCC 1 cut(s) 79
PstI CTGCAG 1 cut(s) 352
PteI GCGCGC 1 cut(s) 382
RruI TCGCGA 1 cut(s) 477
RsaI GTAC 2 cut(s) 5, 284
RsaNI GTAC 2 cut(s) 4, 283
SaqAI TTAA 1 cut(s) 131
SatI GCNGC 3 cut(s) 72, 348, 351
Sau3AI GATC 4 cut(s) 20, 171, 198, 238
Sau96I GGNCC 1 cut(s) 79
SfaNI GCATC 2 cut(s) 131, 406
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 1 cut(s) 79
Sse9I AATT 1 cut(s) 374
SsiI CCGC 2 cut(s) 32, 391
TaaI ACNGT 2 cut(s) 186, 437
TaqI TCGA 1 cut(s) 170
TasI AATT 1 cut(s) 374
TatI WGTACW 1 cut(s) 3
Tru1I TTAA 1 cut(s) 131
Tru9I TTAA 1 cut(s) 131
TscAI CASTG 1 cut(s) 106
TseI GCWGC 3 cut(s) 71, 347, 350
TspDTI ATGAA 3 cut(s) 162, 250, 392
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 79
XceI RCATGY 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.